| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 189,592 | A→G | 40.8% | Q363Q (CAA→CAG) | ldcC → | lysine decarboxylase 2, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 189,592 | 0 | A | G | 40.8% | 27.5 / 53.6 | 76 | Q363Q (CAA→CAG) | ldcC | lysine decarboxylase 2, constitutive |
| Reads supporting (aligned to +/- strand): ref base A (15/30); new base G (15/16); total (30/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.35e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAA > minE/189534‑189645 | aGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCcac > 1:2278314/1‑68 (MQ=255)aGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCcac > 1:2950196/1‑68 (MQ=255) gggTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACaa < 1:1206077/69‑1 (MQ=255) gTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACaaaa > 1:1970603/1‑69 (MQ=255) gTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACaaaa > 1:2556654/1‑69 (MQ=255) gTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACaaaa > 1:2711653/1‑69 (MQ=255) gTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACaaaa > 1:1796988/1‑69 (MQ=255) gTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACaaaa > 1:1662716/1‑69 (MQ=255) gTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACaaaa > 1:2533226/1‑69 (MQ=255) gTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACaaaa > 1:585194/1‑69 (MQ=255) gTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACaaaa > 1:2315135/1‑69 (MQ=255) gTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACaaaa > 1:1249848/1‑69 (MQ=255) gTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACaaaa > 1:712502/1‑69 (MQ=255) gTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACaaa > 1:2103265/1‑68 (MQ=255) aGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGAcc < 1:2115927/48‑1 (MQ=255) aGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGAcc < 1:2366415/48‑1 (MQ=255) aGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGAcc < 1:2041397/48‑1 (MQ=255) aGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGAcc < 1:834198/48‑1 (MQ=255) aGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGAcc < 1:2933852/48‑1 (MQ=255) aGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGAcc < 1:3193605/48‑1 (MQ=255) aGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGAcc < 1:1464669/48‑1 (MQ=255) aGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGAcc < 1:701176/48‑1 (MQ=255) aGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTa > 1:574182/1‑69 (MQ=255) aGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTa > 1:1347004/1‑69 (MQ=255) ggcgAGCGTGTTGCAGGAAAAGTGATCTTCGAAACGCAGTCGACCCACAAAATGCTGGCGGCGTTATCg < 1:2295949/69‑1 (MQ=255) ggcgAGCGTGTTGCAGGAAAAGTGATCTTCGAAACGCAGTCGACCCACAAAATGCTGGCGGCGTTATCg < 1:109310/69‑1 (MQ=255) ggcgAGCGTGTTGCAGGAAAAGTGATCTTCGAAACGCAGTCGACCCACAAAATGCTGGCGGCGTTATCg < 1:2221391/69‑1 (MQ=255) ggcgAGCGTGTTGCAGGAAAAGTGATCTTCGAAACGCAGTCGACCCACAAAATGCTGGCGGCGTTATCg < 1:2190002/69‑1 (MQ=255) ggcgAGCGTGTTGCAGGAAAAGTGATCTTCGAAACGCAGTCGACCCACAAAATGCTGGCGGCGTTATCg < 1:1338841/69‑1 (MQ=255) gcgAGCGTGTTGCAGGAAAAGTGATCTTCGTAACGCAGTCGACCCACAAAATGCTGGCGGCGTTATct < 1:1281597/68‑2 (MQ=255) gcgAGCGTGTTGCAGGAAAAGTGATCTTCGAAACGCAGTCGACCCACAAAATGCTGGCGGCGTTATCg < 1:2710542/68‑1 (MQ=255) gCGGGAAATGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCg < 1:3239273/57‑1 (MQ=255) gCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCg < 1:2335422/57‑1 (MQ=255) gCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCg < 1:2860337/57‑1 (MQ=255) gCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCg < 1:2660847/57‑1 (MQ=255) gCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCg < 1:1767990/57‑1 (MQ=255) gCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCg < 1:214393/57‑1 (MQ=255) gCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCg < 1:2319434/57‑1 (MQ=255) gCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCg < 1:3306359/57‑1 (MQ=255) gCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCg < 1:2263803/57‑1 (MQ=255) gCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCg < 1:758558/57‑1 (MQ=255) gCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCg < 1:2201670/57‑1 (MQ=255) gCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTg < 1:951077/69‑1 (MQ=255) gCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTg < 1:1707793/69‑1 (MQ=255) gCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTg < 1:3082972/69‑1 (MQ=255) gCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTg < 1:2872832/69‑1 (MQ=255) gCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTg < 1:2247072/69‑1 (MQ=255) cGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCg < 1:1951951/56‑1 (MQ=255) aggAAAAGTGATCTTCGAAACGCAGTCGACCCACAAAATGCTGGCGGCGTTATc > 1:346900/2‑54 (MQ=255) aggAAAAGTGATCTTCGAAACGCAGTCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCGTCGCTGa > 1:2230900/2‑68 (MQ=255) aggAAAAGTGATCTTCGAAACGCAGTCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCGTCGCTGa > 1:2192034/2‑68 (MQ=255) aggAAAAGTGATCTTCGAAACGCAGTCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCGTCGCTGa > 1:63278/2‑68 (MQ=255) aggAAAAGTGATCTTCGAAACGCAGTCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCGTCGCTGa > 1:536430/2‑68 (MQ=255) aggAAAAGTGATCTTCGAAACGCAGTCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCGTCGCTGa > 1:357579/2‑68 (MQ=255) aggAAAAGTGATCTTCGAAACGCAGTCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCGTCGCTGa > 1:3309554/2‑68 (MQ=255) aggAAAAGTGATCTTCGAAACGCAGTCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCGTCGCTGa > 1:2366258/2‑68 (MQ=255) aggAAAAGTGATCTTCGAAACGCAGTCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCGTCGCTGa > 1:2369701/2‑68 (MQ=255) aggAAAAGTGATCTTCGAAACGCAGTCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCGTCGCTGa > 1:3182008/2‑68 (MQ=255) aggAAAAGTGATCTTCGAAACGCAGTCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCGTCGCTGa > 1:3084641/2‑68 (MQ=255) aggAAAAGTGATCTTCGAAACGCAGTCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCGTCGCTGa > 1:2527156/2‑68 (MQ=255) aggAAAAGTGATCTTCGAAACGCAGTCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCGTCGCTGa > 1:1711881/2‑68 (MQ=255) aggAAAAGTGATCTTCGAAACGCAGTCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCGTCGCTGa > 1:1823945/2‑68 (MQ=255) aggAAAAGTGATCTTCGAAACGCAGTCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCGTCGCTGa > 1:2907357/2‑68 (MQ=255) aaaaGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCca < 1:1480924/69‑1 (MQ=255) aaaaGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCca < 1:214569/69‑1 (MQ=255) gTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCG‑AGGCTTCGCTGATCCACATTa < 1:2105721/69‑1 (MQ=255) gATCTTCGAAACGCAGTCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCGTCTCTGATCCACATTaa < 1:1236701/69‑1 (MQ=255) gATCTTCGAAACGCAGTCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCGTCGCTGATCCACATTaa < 1:2843994/69‑1 (MQ=255) gATCTTCGAAACGCAGTCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCGTCGCTGATCCACATTaa < 1:858484/69‑1 (MQ=255) gATCTTCGAAACGCAGTCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCGTCGCTGATCCACATTaa < 1:1076258/69‑1 (MQ=255) gATCTTCGAAACGCAGTCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCGTCGCTGATCCACATTaa < 1:2914234/69‑1 (MQ=255) gATCTTCGAAACGCAGTCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCGTCGCTGATCCACATTaa < 1:550792/69‑1 (MQ=255) gATCTTCGAAACGCAGTCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCGTCGCTGATCCACATTaa < 1:35972/69‑1 (MQ=255) gATCTTCGAAACGCAGTCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCGTCGCTGATCCACATTaa < 1:2319149/69‑1 (MQ=255) gATCTTCGAAACGCAGTCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCGTCGCTGATCCACATTaa < 1:3085861/69‑1 (MQ=255) cTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCg < 1:65019/43‑1 (MQ=255) | AGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAA > minE/189534‑189645 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |