| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,183,993 | T→C | 35.9% | G1423G (GGT→GGC) | gltB → | glutamate synthase, large subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,183,993 | 0 | T | C | 35.9% | 46.3 / 44.6 | 75 | G1423G (GGT→GGC) | gltB | glutamate synthase, large subunit |
| Reads supporting (aligned to +/- strand): ref base T (36/12); new base C (23/4); total (59/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.86e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.37e-01 | |||||||||||
GTGGTATCGTCTGCATTCTGGGTAAAACCGGCGTTAACTTCGGTGCGGGCATGACCGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAG > minE/2183932‑2184056 | gtggtATCGTCTGCATTCTGGGTAAAACCGGCGTTAACTTCGGTGCGGGCATGACCGGCGGTTTCGCtt > 1:1462166/1‑69 (MQ=255) tggtATCGTCTGCATTCTGGGTAAAAGCGGCGTTAACTACGGTGCGGGCATGACCGGCGGTTTCGCTTa > 1:2004502/1‑69 (MQ=255) tggtATCGTCTGCATTCTGGGTAAAACCGGCGTTAACTTCGGTGCGGGCATGACCGGCGGTTTCGCTTa > 1:668938/1‑69 (MQ=255) tCGTCTGCATTCTGGGTAAAACCGGCGTTAACTTCGGTGCGGGCATGACCGGCGGTTTCGCTTACGtt > 1:1377704/1‑68 (MQ=255) tCGTCTGCATTCTGGGTAAAACCGGCGTTAACTTCGGTGCGGGCATGACCGGCGGTTTCGCTTACGTtc > 1:539861/1‑69 (MQ=255) tCGTCTGCATTCTGGGTAAAACCGGCGTTAACTTCGGTGCGGGCATGACCGGCGGTTTCGCTTACGTtc > 1:1116383/1‑69 (MQ=255) tCGTCTGCATTCTGGGTAAAACCGGCGTTAACTTCGGTGCGGGCATGACCGGCGGTTTCGCTTACGTtc > 1:18809/1‑69 (MQ=255) gTCTGCATTCTGGGTAAAACCGGCGTTAACTTCGGTGCGGGCATGACCGGCGGTTTCGCTTACGTtctc > 1:1093396/1‑69 (MQ=255) gTCTGCATTCTGGGTAAAACCGGCGTTAACTTCGGTGCGGGCATGACCGGCGGTTTCGCTTACGTtctc > 1:408952/1‑69 (MQ=255) gTCTGCATTCTGGGTAAAACCGGCGTTAACTTCGGTGCGGGCATGACCGGCGGTTTCGCTTACGTtctc > 1:3277690/1‑69 (MQ=255) gTCTGCATTCTGGGTAAAACCGGCGTTAACTTCGGTGCGGGCATGACCGGCGGTTTCGCTTACGTtctc > 1:2713262/1‑69 (MQ=255) gTCTGCATTCTGGGTAAAACCGGCGTTAACTTCGGTGCGGGCATGACCGGCGGTTTCGCTTACGTtctc > 1:2559771/1‑69 (MQ=255) gTCTGCATTCTGGGTAAAACCGGCGTTAACTTCGGTGCGGGCATGACCGGCGGTTTCGCTTACGTtctc > 1:2454631/1‑69 (MQ=255) gTCTGCATTCTGGGTAAAACCGGCGTTAACTTCGGTGCGGGCATGACCGGCGGTTTCGCTTACGTtctc > 1:1950041/1‑69 (MQ=255) gTCTGCATTCTGGGTAAAACCGGCGTTAACTTCGGTGCGGGCATGACCGGCGGTTTCGCTTACGTtctc > 1:151409/1‑69 (MQ=255) gTCTGCATTCTGGGTAAAACCGGAGTTAACTTCGGTGCGGGCATGACCGGCGGTTTCGCTTACGTtctc > 1:1594196/1‑69 (MQ=255) tGCATTCTGGGTAAAACCGGCGTTAACTTCGGTGCGGGCATGACCGGCGGTTTCGCTTACGTTCTCGAt < 1:11433/69‑1 (MQ=255) tGCATTCTGGGTAAAACCGGCGTTAACTTCGGTGCGGGCATGACCGGCGGTTTCGCTTACGTTCTCGAt < 1:63892/69‑1 (MQ=255) cTGGGTAAAACCGGCGTTAACTTCGGTGCGGGCATGACCGGCGGTTTCGCTTACGTTCTCGATGAAAgc > 1:1389896/1‑69 (MQ=255) cTGGGTAAAACCGGCGTTAACTTCGGTGCGGGCATGACCGGCGGTTTCGCTTACGTTCTCGATGAAAgc > 1:812544/1‑69 (MQ=255) cTGGGTAAAACCGGCGTTAACTTCGGTGCGGGCATGACCGGCGGTTTCGCTTACGTTCTCGATGAAAgc > 1:528176/1‑69 (MQ=255) cTGGGTAAAACCGGCGTTAACTTCGGTGCGGGCATGACCGGCGGTTTCGCTTACGTTCTCGATGAAAg > 1:2502503/1‑68 (MQ=255) cTGGGTAAAACCGGCGTTAACTTCGGCGCGGGCATGACCGGTGGCTTCGCTTACGTTCTCGATGAAAgc < 1:1082862/69‑1 (MQ=255) cTGGGTAAAACCGGCGTTAACTTCGGCGCGGGCATGACCGGTGGCTTCGCTTACGTTCTCGATGAAAgc < 1:3138120/69‑1 (MQ=255) cTGGGTAAAACCGGCGTTAACTTCGGCGCGGGCATGACCGGTGGCTTCGCTTACGTTCTCGATGAAAgc < 1:786047/69‑1 (MQ=255) tGGGTAAAACCGGCGTTAACTTCGGCGCGGGCATGACCGGTGGCTTCGCTTACGTTCTCGATGAAAgc < 1:1411166/68‑1 (MQ=255) aaaaCCGGCGTTAACTTCGGTGCGGGCATGACCGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGAt > 1:1976468/1‑69 (MQ=255) aaaaCCGGCGTTAACTTCGGTGCGGGCATGACCGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGAt > 1:1711929/1‑69 (MQ=255) tAACTTCGGTGCGGGCATGACCGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAAcg < 1:1573930/69‑1 (MQ=255) aaCTTCGGTGCGGGCATGACCGGCGGTTTCGCTTACGTTCTCGATGAAAgc > 1:2113963/1‑51 (MQ=255) aaCTTCGGTGCGGGCATGACCGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAAcgc > 1:2289564/1‑69 (MQ=255) aaCTTCGGTGCGGGCATGACCGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAAcgc > 1:2625508/1‑69 (MQ=255) aaCTTCGGTGCGGGCATGACCGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAAcgc > 1:467835/1‑69 (MQ=255) aaCTTCGGTGCGGGCATGACCGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAAcgc > 1:3127318/1‑69 (MQ=255) aaCTTCGGTGCGGGCATGACCGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAAcgc > 1:2252162/1‑69 (MQ=255) aaCTTCGGTGCGGGCATGACCGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAAcgc > 1:2153543/1‑69 (MQ=255) aaCTTCGGTGCGGGCATGACCGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAAcgc > 1:93903/1‑69 (MQ=255) aaCTTCGGTGCGGGCATGACCGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAAcgc > 1:944283/1‑69 (MQ=255) aaCTTCGGTGCGGGCATCACCGGCGGTTTCGCTTACGtt > 1:3176537/1‑39 (MQ=255) cGGCGCGGGCATGACCTGTGGCTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTaa > 1:175930/1‑69 (MQ=255) cGGCGCGGGCATGACCGGTGGCTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTaa > 1:241118/1‑69 (MQ=255) cGGCGCGGGCATGACCGGTGGCTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTaa > 1:2457583/1‑69 (MQ=255) cGGCGCGGGCATGACCGGTGGCTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTaa > 1:681821/1‑69 (MQ=255) cGGCGCGGGCATGACCGGTGGCTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTaa > 1:1262471/1‑69 (MQ=255) cGGCGCGGGCATGACCGGTGGCTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTaa > 1:1903137/1‑69 (MQ=255) cGGCGCGGGCATGACCGGTGGCTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTaa > 1:3424001/1‑69 (MQ=255) cGGCGCGGGCATGACCGGTGGCTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTaa > 1:1192096/1‑69 (MQ=255) cGGCGCGGGCATGACCGGTGGCTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTaa > 1:3188512/1‑69 (MQ=255) cGGGCATGACCGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCgg < 1:947451/69‑1 (MQ=255) gACCGGTGGCTTCGCTTACGTTCTCGATGAAAGGGGCGATTTCCGCAAACGCGTTAACCCGGAACTgg > 1:2753300/1‑68 (MQ=255) gACCGGTGGCTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTa > 1:1289872/1‑56 (MQ=255) gACCGGTGGCTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTgg > 1:1567722/1‑68 (MQ=255) gACCGGTGGCTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTgg > 1:1548050/1‑68 (MQ=255) gACCGGTGGCTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTgg > 1:1904398/1‑68 (MQ=255) gACCGGTGGCTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTgg > 1:901198/1‑68 (MQ=255) gACCGGTGGCTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTgg > 1:1934879/1‑68 (MQ=255) gACCGGTGGCTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTgg > 1:791957/1‑68 (MQ=255) gACCGGTGGCTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTgg > 1:2325533/1‑68 (MQ=255) gACCGGTGGCTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTgg > 1:612398/1‑68 (MQ=255) gACCGGTGGCTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTgg > 1:2727397/1‑68 (MQ=255) gACCGGTGGCTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTgg > 1:2771311/1‑68 (MQ=255) gACCGGTGGCTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTgg > 1:33060/1‑68 (MQ=255) gACCGGTGGCTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTg > 1:360329/1‑67 (MQ=255) aCCGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCa > 1:390184/1‑46 (MQ=255) aCCGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGa > 1:778827/1‑62 (MQ=255) aCCGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTgg > 1:2639088/1‑67 (MQ=255) aCCGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTgg > 1:3204979/1‑67 (MQ=255) ggcggTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCTAg < 1:111562/69‑1 (MQ=255) ggcggTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAg < 1:361075/69‑1 (MQ=255) ggcggTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAg < 1:2264328/69‑1 (MQ=255) ggcggTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAg < 1:747419/69‑1 (MQ=255) ggcggTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAg < 1:2091619/69‑1 (MQ=255) ggcggTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAg < 1:350340/69‑1 (MQ=255) ggcggTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAg < 1:829408/69‑1 (MQ=255) gcggTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAg < 1:2865398/68‑1 (MQ=255) | GTGGTATCGTCTGCATTCTGGGTAAAACCGGCGTTAACTTCGGTGCGGGCATGACCGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAG > minE/2183932‑2184056 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |