| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,185,556 | C→T | 48.4% | I422I (ATC→ATT) | gltD → | glutamate synthase, 4Fe‑4S protein, small subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,185,556 | 0 | C | T | 48.4% | ‑3.9 / 24.5 | 31 | I422I (ATC→ATT) | gltD | glutamate synthase, 4Fe‑4S protein, small subunit |
| Reads supporting (aligned to +/- strand): ref base C (6/10); new base T (8/7); total (14/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.79e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.41e-01 | |||||||||||
CCGTCCACACAACATGGAATGGCTGGCAAAACACAGCGTCGAGCTGGATTCACAAGGCCGCATCATCGCCCCGGAAGGCAGCGACAACGCCTTCCAGACCAGCAACCCGAAAATCTTTGCTGGCG > minE/2185493‑2185617 | ccGTCCACACCACATGGAATGGCTGGCAAAACACAGCGTCGAGCTGGATTCGCAGGGGCGCATTATCGc > 1:2572014/1‑69 (MQ=255)ccGTCCACACAACATGGAATGGCTGGCAAAACACAGCGTCGAGCTGGATTCGCAGGGGCGCATTATCg > 1:1375509/1‑68 (MQ=255)ccGTCCACACAACATGGAATGGCTGGCAAAACACAGCGTCGAGCTGGATTCGCAGGGGCGCATTATCGc > 1:834342/1‑69 (MQ=255)ccGTCCACACAACATGGAATGGCTGGCAAAACACAGCGTCGAGCTGGATTCGCAGGGGCGCATTATCGc > 1:795272/1‑69 (MQ=255)ccGTCCACACAACATGGAATGGCTGGCAAAACACAGCGTCGAGCTGGATTCGCAGGGGCGCATTATCGc > 1:395934/1‑69 (MQ=255)ccGTCCACACAACATGGAATGGCTGGCAAAACACAGCGTCGAGCTGGATTCGCAGGGGCGCATTATCGc > 1:2809230/1‑69 (MQ=255)ccGTCCACACAACATGGAATGGCTGGCAAAACACAGCGTCGAGCTGGATTCGCAGGGGCGCATTATCGc > 1:2067842/1‑69 (MQ=255)ccGTCCACACAACATGGAATGGCTGGCAAAACACAGCGTCGAGCTGGATTCGCAGGGGCGCATTATCGc > 1:1953180/1‑69 (MQ=255) aTGGAATGGCTGGCAAAACACAGCGTCGAGCTGGATTCACAAGGCCGCATCATCGCCCCGGAAGGCAGc > 1:1961855/1‑69 (MQ=255) gAATGGCTGGCAAAACACAGCGTCGAGCTGGATTCACAAGGCCGCATCATCGCCCCGGAAGGCAGCGa > 1:1762203/1‑68 (MQ=255) gAATGGCTGGCAAAACACAGCGTCGAGCTGGATTCACAAGGCCGCATCATCGCCCCGGAAGGCAGCGAc < 1:1167568/69‑1 (MQ=255) gAATGGCTGGCAAAACACAGCGTCGAGCTGGATTCACAAGGCCGCATCATCGCCCCGGAAGGCAGCGAc < 1:3212938/69‑1 (MQ=255) gAATGGCTGGCAAAACACAGCGTCGAGCTGGATTCACAAGGCCGCATCATCGCCCCGGAAGGCAGCGAc < 1:1889820/69‑1 (MQ=255) gAATGGCTGGCAAAACACAGCGTCGAGCTGGATTCACAAGGCCGCATCATCGCCCCGGAAGGCAGCGAc > 1:1603559/1‑69 (MQ=255) gAATGGCTGGCAAAACACAGCGTCGAGCTGGATTCACAAGGCCGCATCATCGCCCCGGAAGGCAGCGAc < 1:1860244/69‑1 (MQ=255) ggctggcAAAACACAGCGTCGAGCTGGATTCACAAGGCCGCATCATCGCCCCGGAAGGCAGCGACAACg > 1:1722978/1‑69 (MQ=255) aGCGTCGAGCTGGATTCACAAGGCCGCATCATCGCCCCGGAAGGCAGCGACAACGCCTTCCAGACCAGc < 1:519660/69‑1 (MQ=255) gAGCTGGATTCACAAGGCCGCATCATCGCCCCGGAAGGCAGCGACAACGCCTTCCAGACCAGCAAccc > 1:2860866/1‑68 (MQ=255) cacaAGGCCGCATCATCGCCCCGGAAGGCAGCGACAACGCCTTCCAGACCAGCAACCCGAAAATCTTTg > 1:3157557/1‑69 (MQ=255) acaAGGCCGCATCATCGCCCCGGAAGGCAGCGACAACGCCTTCCAGACCAGCAACCCGAAAATCTTTg < 1:2089602/68‑1 (MQ=255) aaGGCCGCATCATCGCCCCGGAAGGCAGCGACAACGCCTTCCAGACCAGCAACCCGAAAATCTTTGCt < 1:1687920/68‑1 (MQ=255) aaGGCCGCATCATCGCCCCGGAAGGCAGCGACAACGCCTTCCAGACCAGCAACCCGAAAATCTTTGCt < 1:451007/68‑1 (MQ=255) aaGGCCGCATCATCGCCCCGGAAGGCAGCGACAACGCCTTCCAGACCAGCAACCCGAAAATCTTTGCt < 1:260541/68‑1 (MQ=255) gggcGCATTATCGCCCCGGAAGACAACGACAACGCTTTCCAGACCAGCAACCCGAAAATCTTTGCTggc < 1:1764921/66‑1 (MQ=255) gggcGCATTATCGCCCCGGAAGACAACGACAACGCTTTCCAGACCAGCAACCCGAAAATCTTTGCTggc < 1:417481/66‑1 (MQ=255) gggcGCATTATCGCCCCGGAAGACAACGACAACGCTTTCCAGACCAGCAACCCGAAAATCTTTGCTggc < 1:601082/66‑1 (MQ=255) gggcGCATTATCGCCCCGGAAGACAACGACAACGCTTTCCAGACCAGCAACCCGAAAATCTTTGCTggc < 1:773810/66‑1 (MQ=255) gggcGCATTATCGCCCCGGAAGACAACGACAACGCTTTCCAGACCAGCAACCCGAAAATCTTTGCTggc < 1:2231906/66‑1 (MQ=255) gggcGCATTATCGCCCCGGAAGACAACGACAACGCTTTCCAGACCAGCAACCCGAAAATCTTTGCTggc < 1:1061369/66‑1 (MQ=255) gggcGCATTATCGCCCCGGAAGACAACGACAACGCTTTCCAGACCAGCAACCCGAAAATCTTTGCTggc < 1:931477/66‑1 (MQ=255) gCCGCATCATCGCCCCGGAAGGCAGCGACAACGCCTTCCAGACCAGCAACCCGAAAATCTTTGCTggcg < 1:2628020/69‑1 (MQ=255) | CCGTCCACACAACATGGAATGGCTGGCAAAACACAGCGTCGAGCTGGATTCACAAGGCCGCATCATCGCCCCGGAAGGCAGCGACAACGCCTTCCAGACCAGCAACCCGAAAATCTTTGCTGGCG > minE/2185493‑2185617 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |