| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,188,466 | A→C | 44.8% | G131G (GGT→GGG) | nanA ← | N‑acetylneuraminate lyase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,188,466 | 0 | A | C | 44.8% | ‑1.5 / 117.8 | 103 | G131G (GGT→GGG) | nanA | N‑acetylneuraminate lyase |
| Reads supporting (aligned to +/- strand): ref base A (23/34); new base C (13/33); total (36/67) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.19e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.66e-02 | |||||||||||
Only 100 of 106 total aligned reads displayed.
TTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTTCAAAGCTGAAAGGATAGTAG > minE/2188401‑2188532 | ttGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAAcc < 1:2277797/68‑1 (MQ=255) ccAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGaa > 1:485107/1‑68 (MQ=255) ccAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGaa > 1:759570/1‑68 (MQ=255) ccAGGGTCAGTTTTACCCCACTCAGGGATGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGaa > 1:1241352/1‑68 (MQ=255) gTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCaata > 1:3207905/1‑69 (MQ=255) gTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCaata > 1:2130791/1‑69 (MQ=255) gTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCaata > 1:3348664/1‑69 (MQ=255) ttACCCCGCTCAGGGCTGGAATGTTGTACACCACCATCGGCAACCCATCCGCCGAATCAATAATTGccc > 1:2246997/1‑69 (MQ=255) ttACCCCGCTCAGGGCTGGAATGTTGTACACCACCATCGGCAACCCATCCGCCGAATCAATAATTGccc > 1:1462338/1‑69 (MQ=255) ttACCCCGCTCAGGGCTGGAATGTTGTACACCACCATCGGCAACCCATCCGCCGAATCAATAATTGccc > 1:3026298/1‑69 (MQ=255) ttACCCCGCTCAGGGCTGGAATGTTGTACACCACCATCGGCAACCCATCCGCCGAATCAATAATTGccc > 1:1864123/1‑69 (MQ=255) ttACCCCGCTCAGGGCTGGAATGTTGTACACCACCATCGGCAACCCATCCGCCGAATCAATAATTGccc > 1:515358/1‑69 (MQ=255) ttACCCCGCTCAGGGCTGGAATGTTGTACACCACCATCGGCAACCCATCCGCCGAATCAATAATTGccc > 1:524341/1‑69 (MQ=255) ttACCCCGCTCAGGGCTGGAATGTTGTACACCACCATCGGCAACCCATCCGCCGAATCAATAATTGccc > 1:698378/1‑69 (MQ=255) ttACCCCGCTCAGGGCTGGAATGTTGTACACCACCATCGGCAACCCATCCGCCGAATCAATAATTGccc > 1:1092132/1‑69 (MQ=255) ttACCCCGCTCAGGGCTGGAATGTTGTACACCACCATCGGCAACCCATACGCCGAATCAATAATTGccc > 1:53421/1‑69 (MQ=255) ttACCCCGCTCAGGGCTGGAATGTTGTACACCACCATCGGCAACCCATACGCCGAATCAATAATTGccc > 1:1442237/1‑69 (MQ=255) ttACCCAGCTCAGGGCTGGAATGTTGTACACCACCATCGGCAACCCATCCGCCGAATCAATAATTGccc > 1:3467/1‑69 (MQ=255) cccACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGAt < 1:993123/68‑1 (MQ=255) cccACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGAt < 1:2457971/68‑1 (MQ=255) cccACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGAt < 1:1778001/68‑1 (MQ=255) cccACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGAt < 1:2106412/68‑1 (MQ=255) cccACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGAt < 1:2794730/68‑1 (MQ=255) cccACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGAt < 1:1727271/68‑1 (MQ=255) cccACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGAt < 1:1571536/68‑1 (MQ=255) cccACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGAt < 1:3227598/68‑1 (MQ=255) cccACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGAt < 1:1407332/68‑1 (MQ=255) cccACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGAt < 1:550657/68‑1 (MQ=255) cccACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGAt < 1:764262/68‑1 (MQ=255) cccACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGAt < 1:863671/68‑1 (MQ=255) cccACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGAt < 1:977157/68‑1 (MQ=255) ccACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGAt < 1:164996/67‑1 (MQ=255) cAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGAt > 1:3373712/1‑68 (MQ=255) cAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGAt > 1:239471/1‑68 (MQ=255) gCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTg < 1:3202843/62‑1 (MQ=255) gCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTg < 1:2999319/62‑1 (MQ=255) gCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTg < 1:3389771/62‑1 (MQ=255) gCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTg < 1:2930626/62‑1 (MQ=255) gCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTg < 1:824288/62‑1 (MQ=255) gCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTg < 1:1036347/62‑1 (MQ=255) gCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTg < 1:1051878/62‑1 (MQ=255) gCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTg < 1:1070882/62‑1 (MQ=255) gCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTg < 1:1174105/62‑1 (MQ=255) gCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTg < 1:135499/62‑1 (MQ=255) gCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTg < 1:1419431/62‑1 (MQ=255) cTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTg < 1:2430030/61‑1 (MQ=255) cTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAgt > 1:959374/1‑69 (MQ=255) cTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAgt > 1:2875188/1‑69 (MQ=255) cTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAgt > 1:2506399/1‑69 (MQ=255) tGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAgtg < 1:2735774/69‑1 (MQ=255) tGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAgtg < 1:2982448/69‑1 (MQ=255) ggAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCaat > 1:807515/1‑44 (MQ=255) ggAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCaat > 1:251797/1‑44 (MQ=255) ggAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCaat > 1:731071/1‑44 (MQ=255) ggAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCaat > 1:686740/1‑44 (MQ=255) gtACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGc > 1:106665/1‑56 (MQ=255) gtACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGc > 1:1638568/1‑56 (MQ=255) gtACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGc > 1:315074/1‑56 (MQ=255) gtACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGc > 1:305040/1‑56 (MQ=255) gtACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTTCaa < 1:1470787/68‑1 (MQ=255) caccaTCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTTCAAAGCTGat < 1:569110/69‑2 (MQ=255) tCGGCAACCCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTTCAAAGCTGAAAGGAt < 1:1218913/69‑1 (MQ=255) tCGGCAACCCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTTCAAAGCTGAAAGGAt < 1:13022/69‑1 (MQ=255) tCGGCAACCCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTTCAAAGCTGAAAGGAt < 1:424502/69‑1 (MQ=255) tCGGCAACCCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTTCAAAGCTGAAAGGAt < 1:2463029/69‑1 (MQ=255) tCGGCAACCCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTTCAAAGCTGAAAGGAt < 1:423784/69‑1 (MQ=255) tCGGCAACCCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTTCAAAGCTGAAAGGAt < 1:1158154/69‑1 (MQ=255) tCGGCAACCCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTTCAAAGCTGAAAGGAt < 1:653706/69‑1 (MQ=255) tCGGCAACCCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTTCAAAGCTGAAAGGAt < 1:67243/69‑1 (MQ=255) tCGGCAACCCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTTCAAAGCTGAAAGGAt < 1:1334725/69‑1 (MQ=255) tCGGCAACCCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTTCAAAGCTGAAAGGAt < 1:744736/69‑1 (MQ=255) tCGGCAACCCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTTCAAAGCTGAAAGGAt < 1:1153779/69‑1 (MQ=255) tCGGCAACCCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTTCAAAGCTGAAAGGAt < 1:1145309/69‑1 (MQ=255) tCGGCAACCCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTTCAAAGCTGAAAGGAt < 1:2117226/69‑1 (MQ=255) tCGGCAACCCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTTCAAAGCTGAAAGGAt < 1:1025464/69‑1 (MQ=255) tCGGCAACCCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTTCAAAGCTGAAAGGAt < 1:2697104/69‑1 (MQ=255) tCGGCAACCCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTTCAAAGCTGAAAGGAt < 1:2985054/69‑1 (MQ=255) tCGGCAACCCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTTCAAAGCTGAAAGGAt < 1:1803718/69‑1 (MQ=255) tCGGCAACCCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTTCAAAGCTGAAAGGAt < 1:1760007/69‑1 (MQ=255) tCGGCAACCCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTTCAAAGCTGAAAGGAt < 1:3057669/69‑1 (MQ=255) tCGGCAACCCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTTCAAAGCTGAAAGGAt < 1:3066472/69‑1 (MQ=255) tCGGCAACCCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTTCAAAGCTGAAAGGAt < 1:374298/69‑1 (MQ=255) tCGGCAACCCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTTCAAAGCTGAAAGGAt < 1:3205166/69‑1 (MQ=255) tCGGCAACCCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTTCAAAGCTGAAAGGAt < 1:2946734/69‑1 (MQ=255) tCGGCAACCCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTTCAAAGCTGAAAGGAt < 1:3337915/69‑1 (MQ=255) tCGGCAACCCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTTCAAAGCTGAAAGGAt < 1:2931248/69‑1 (MQ=255) tCGGCAACCCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTTCAAAGCTGAAAGGAt < 1:2841355/69‑1 (MQ=255) tCGGCAACCCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTTCAAAGCTGAAAGGAt < 1:3355748/69‑1 (MQ=255) tCGGCAACCCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTTCAAAGCTGAAAGGAt < 1:2113810/69‑1 (MQ=255) tCGGCAACCCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTTCAAAGCTGAAAGGAt < 1:3390538/69‑1 (MQ=255) tCGGCAACCCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTTCAAAGCTGAAAGGAt < 1:2544724/69‑1 (MQ=255) tCGGCAACCCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTTCAAACCTGAAAGGAt < 1:604466/69‑1 (MQ=255) tCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTTCAAAGCTGAAAGGAt < 1:677499/69‑1 (MQ=255) cGGCAACCCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTTCAAAGCTGAAAGGAt < 1:2358475/68‑1 (MQ=255) cGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAgtg < 1:36086/48‑1 (MQ=255) ggCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTTCAAAGCTGAAAGGAt > 1:1983668/1‑67 (MQ=255) ggCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTTCAAAGCTGAAAGGAt > 1:1214085/1‑67 (MQ=255) aaaCCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTTCAAAGCTGAAAGGAtagtag < 1:1468880/69‑1 (MQ=255) aaaCCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTTCAAAGCTGAAAGGAtagtag < 1:1452565/69‑1 (MQ=255) aaaCCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTTCAAAGCTGAAAGGAtagtag < 1:1271043/69‑1 (MQ=255) | TTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTTCAAAGCTGAAAGGATAGTAG > minE/2188401‑2188532 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |