| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 189,628 | T→G | 42.8% | A375A (GCT→GCG) | ldcC → | lysine decarboxylase 2, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 189,628 | 0 | T | G | 42.8% | 10.5 / 56.4 | 56 | A375A (GCT→GCG) | ldcC | lysine decarboxylase 2, constitutive |
| Reads supporting (aligned to +/- strand): ref base T (10/22); new base G (14/10); total (24/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.81e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATACCAC > minE/189566‑189696 | gCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTg < 1:2247072/69‑1 (MQ=255)gCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTg < 1:951077/69‑1 (MQ=255)gCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTg < 1:3082972/69‑1 (MQ=255)gCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTg < 1:1707793/69‑1 (MQ=255)gCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTg < 1:2872832/69‑1 (MQ=255) aggAAAAGTGATCTTCGAAACGCAGTCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCGTCGCTGa > 1:2907357/2‑68 (MQ=255) aggAAAAGTGATCTTCGAAACGCAGTCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCGTCGCTGa > 1:2192034/2‑68 (MQ=255) aggAAAAGTGATCTTCGAAACGCAGTCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCGTCGCTGa > 1:2230900/2‑68 (MQ=255) aggAAAAGTGATCTTCGAAACGCAGTCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCGTCGCTGa > 1:2366258/2‑68 (MQ=255) aggAAAAGTGATCTTCGAAACGCAGTCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCGTCGCTGa > 1:2527156/2‑68 (MQ=255) aggAAAAGTGATCTTCGAAACGCAGTCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCGTCGCTGa > 1:63278/2‑68 (MQ=255) aggAAAAGTGATCTTCGAAACGCAGTCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCGTCGCTGa > 1:2369701/2‑68 (MQ=255) aggAAAAGTGATCTTCGAAACGCAGTCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCGTCGCTGa > 1:3084641/2‑68 (MQ=255) aggAAAAGTGATCTTCGAAACGCAGTCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCGTCGCTGa > 1:3182008/2‑68 (MQ=255) aggAAAAGTGATCTTCGAAACGCAGTCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCGTCGCTGa > 1:3309554/2‑68 (MQ=255) aggAAAAGTGATCTTCGAAACGCAGTCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCGTCGCTGa > 1:357579/2‑68 (MQ=255) aggAAAAGTGATCTTCGAAACGCAGTCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCGTCGCTGa > 1:536430/2‑68 (MQ=255) aggAAAAGTGATCTTCGAAACGCAGTCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCGTCGCTGa > 1:1823945/2‑68 (MQ=255) aggAAAAGTGATCTTCGAAACGCAGTCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCGTCGCTGa > 1:1711881/2‑68 (MQ=255) aaaaGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCca < 1:214569/69‑1 (MQ=255) aaaaGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCca < 1:1480924/69‑1 (MQ=255) gTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCG‑AGGCTTCGCTGATCCACATTa < 1:2105721/69‑1 (MQ=255) gATCTTCGAAACGCAGTCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCGTCTCTGATCCACATTaa < 1:1236701/69‑1 (MQ=255) gATCTTCGAAACGCAGTCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCGTCGCTGATCCACATTaa < 1:2843994/69‑1 (MQ=255) gATCTTCGAAACGCAGTCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCGTCGCTGATCCACATTaa < 1:35972/69‑1 (MQ=255) gATCTTCGAAACGCAGTCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCGTCGCTGATCCACATTaa < 1:1076258/69‑1 (MQ=255) gATCTTCGAAACGCAGTCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCGTCGCTGATCCACATTaa < 1:3085861/69‑1 (MQ=255) gATCTTCGAAACGCAGTCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCGTCGCTGATCCACATTaa < 1:858484/69‑1 (MQ=255) gATCTTCGAAACGCAGTCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCGTCGCTGATCCACATTaa < 1:2914234/69‑1 (MQ=255) gATCTTCGAAACGCAGTCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCGTCGCTGATCCACATTaa < 1:550792/69‑1 (MQ=255) gATCTTCGAAACGCAGTCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCGTCGCTGATCCACATTaa < 1:2319149/69‑1 (MQ=255) tCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGaa < 1:77306/69‑1 (MQ=255) tCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGaa < 1:1027204/69‑1 (MQ=255) tCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGaa < 1:3369657/69‑1 (MQ=255) tCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGaa < 1:1110660/69‑1 (MQ=255) tCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGaa < 1:2703795/69‑1 (MQ=255) tCGACCCACAAAATCCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGaa < 1:1825723/69‑1 (MQ=255) ggcggcGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGAAGAGGCCTTTAACGAAGc < 1:3247339/69‑1 (MQ=255) ggcggcGTTATCGCAGGCGTCGCTGATCCACATTaa < 1:2886920/36‑1 (MQ=255) gTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGAAGAGGCCTTTAACGAAGCCTTTat < 1:2280492/69‑1 (MQ=255) aTCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGaa > 1:2957148/1‑43 (MQ=255) aTCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGaa > 1:2867877/1‑43 (MQ=255) aTCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGaa > 1:2468252/1‑43 (MQ=255) cGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGAAGAGGCCTTTAACGAAGCCTTTatgatg > 1:1469601/1‑68 (MQ=255) cGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGAAGAGGCCTTTAACGAAGCCTTTatgatg > 1:1420048/1‑68 (MQ=255) cGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGAAGAGGCCTTTAACGAAGCCTTTatgatg > 1:2620747/1‑68 (MQ=255) cGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGAAGAGGCCTTTAACGAAGCCTTTatgatg > 1:2319810/1‑68 (MQ=255) cGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGAAGAGGCCTTTAACGAAGCCTTTatgatg > 1:722172/1‑68 (MQ=255) cGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGAAGAGGCCTTTAACGAAGCCTTTatgatg > 1:2492393/1‑68 (MQ=255) cGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGAAGAGGCCTTTAACGAAGCCTTTatgat > 1:1074023/1‑67 (MQ=255) aGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCAt < 1:540195/68‑1 (MQ=255) aGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCAt < 1:2625063/68‑1 (MQ=255) aGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCAt < 1:1351244/68‑1 (MQ=255) aGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCAt < 1:1305529/68‑1 (MQ=255) aGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCAt < 1:941116/68‑1 (MQ=255) aGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCAt < 1:1283640/68‑1 (MQ=255) ttCGCTGATCCACATTAAAGGCGAGTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATaccac < 1:2580684/69‑1 (MQ=255) ttCGCTGATCCACATTAAAGGCGAGTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATaccac < 1:2075475/69‑1 (MQ=255) | GCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATACCAC > minE/189566‑189696 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |