| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,188,658 | G→A | 76.0% | A67A (GCC→GCT) | nanA ← | N‑acetylneuraminate lyase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,188,658 | 0 | G | A | 76.0% | 106.2 / 29.2 | 87 | A67A (GCC→GCT) | nanA | N‑acetylneuraminate lyase |
| Reads supporting (aligned to +/- strand): ref base G (5/16); new base A (16/50); total (21/66) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
GGCTTTCGGCGGTGCTGACGCAACCGACGTGGGCGATGAGTTTAATCTTACCTTTCGCCTCTTCGGCGACGATTTCCAGTACCTGTTCACGCTCGGAAAGGCTTTGTACAA > minE/2188594‑2188704 | ggCTTTCGGCGGTGCTGACGCAACCGACGTGGGCGATGAGTTTAATCTTACCTTTCGCCTCTTCGGcg > 1:3240806/1‑68 (MQ=255) ggcggTGCTGACGCAACCGACGTGGGCGATGAGTTTAATCTTACCTTTCGCCTCTTCAGCGACGAtt < 1:2958118/67‑1 (MQ=255) ggcggTGCTGACGCAACCGACGTGGGCGATGAGTTTAATCTTACCTTTCGCCTCTTCAGCGACGAtt < 1:1858030/67‑1 (MQ=255) ggcggTGCTGACGCAACCGACGTGGGCGATGAGTTTAATCTTACCTTTCGCCTCTTCAGCGACGAtt < 1:1874101/67‑1 (MQ=255) ggcggTGCTGACGCAACCGACGTGGGCGATGAGTTTAATCTTACCTTTCGCCTCTTCAGCGACGAtt < 1:3325512/67‑1 (MQ=255) ggcggTGCTGACGCAACCGACGTGGGCGATGAGTTTAATCTTACCTTTCGCCTCTTCAGCGACGAtt < 1:3164245/67‑1 (MQ=255) ggcggTGCTGACGCAACCGACGTGGGCGATGAGTTTAATCTTACCTTTCGCCTCTTCAGCGACGAtt < 1:3160121/67‑1 (MQ=255) ggcggTGCTGACGCAACCGACGTGGGCGATGAGTTTAATCTTACCTTTCGCCTCTTCAGCGACGAtt < 1:3128660/67‑1 (MQ=255) ggcggTGCTGACGCAACCGACGTGGGCGATGAGTTTAATCTTACCTTTCGCCTCTTCAGCGACGAtt < 1:2191818/67‑1 (MQ=255) ggcggTGCTGACGCAACCGACGTGGGCGATGAGTTTAATCTTACCTTTCGCCTCTTCAGCGACGAtt < 1:2201400/67‑1 (MQ=255) ggcggTGCTGACGCAACCGACGTGGGCGATGAGTTTAATCTTACCTTTCGCCTCTTCAGCGACGAtt < 1:2028501/67‑1 (MQ=255) ggcggTGCTGACGCAACCGACGTGGGCGATGAGTTTAATCTTACCTTTCGCCTCTTCAGCGACGAtt < 1:2938942/67‑1 (MQ=255) ggcggTGCTGACGCAACCGACGTGGGCGATGAGTTTAATCTTACCTTTCGCCTCTTCAGCGACGAtt < 1:2362181/67‑1 (MQ=255) ggcggTGCTGACGCAACCGACGTGGGCGATGAGTTTAATCTTACCTTTCGCCTCTTCAGCGACGAtt < 1:2420755/67‑1 (MQ=255) ggcggTGCTGACGCAACCGACGTGGGCGATGAGTTTAATCTTACCTTTCGCCTCTTCAGCGACGAtt < 1:2422627/67‑1 (MQ=255) ggcggTGCTGACGCAACCGACGTGGGCGATGAGTTTAATCTTACCTTTCGCCTCTTCAGCGACGAtt < 1:284619/67‑1 (MQ=255) ggcggTGCTGACGCAACCGACGTGGGCGATGAGTTTAATCTTACCTTTCGCCTCTTCAGCGACGAtt < 1:2474555/67‑1 (MQ=255) ggcggTGCTGACGCAACCGACGTGGGCGATGAGTTTAATCTTACCTTTCGCCTCTTCAGCGACGAtt < 1:2479301/67‑1 (MQ=255) ggcggTGCTGACGCAACCGACGTGGGCGATGAGTTTAATCTTACCTTTCGCCTCTTCAGCGACGAtt < 1:2572171/67‑1 (MQ=255) ggcggTGCTGACGCAACCGACGTGGGCGATGAGTTTAATCTTACCTTTCGCCTCTTCAGCGACGAtt < 1:1164859/67‑1 (MQ=255) ggcggTGCTGACGCAACCGACGTGGGCGATGAGTTTAATCTTACCTTTCGCCTCTTCAGCGACGAtt < 1:1186278/67‑1 (MQ=255) ggcggTGCTGACGCAACCGACGTGGGCGATGAGTTTAATCTTACCTTTCGCCTCTTCAGCGACGAtt < 1:893464/67‑1 (MQ=255) ggcggTGCTGACGCAACCGACGTGGGCGATGAGTTTAATCTTACCTTTCGCCTCTTCAGCGACGAtt < 1:841111/67‑1 (MQ=255) ggcggTGCTGACGCAACCGACGTGGGCGATGAGTTTAATCTTACCTTTCGCCTCTTCAGCGACGAtt < 1:1561711/67‑1 (MQ=255) ggcggTGCTGACGCAACCGACGTGGGCGATGAGTTTAATCTTACCTTTCGCCTCTTCAGCGACGAtt < 1:1566407/67‑1 (MQ=255) ggcggTGCTGACGCAACCGACGTGGGCGATGAGTTTAATCTTACCTTTCGCCTCTTCAGCGACGAtt < 1:1659998/67‑1 (MQ=255) ggcggTGCTGACGCAACCGACGTGGGCGATGAGTTTAATCTTACCTTTCGCCTCTTCAGCGACGAtt < 1:1768034/67‑1 (MQ=255) ggcggTGCTGACGCAACCGACGTGGGCGATGAGTTTAATCTTACCTTTCGCCTCTTCAGCGACGAtt < 1:1707048/67‑1 (MQ=255) gcggTGCTGACGCAACCGATGTGGGCGATGAGTTTAATCTTACCTTTCGCCTCTTCGGCGACGATTTc < 1:2978013/68‑1 (MQ=255) gcggTGCTGACGCAACCGACGTGGGCGATGAGTTTAATCTTACCTTTCGCCTCTTCGGCGACGATTTc < 1:332560/68‑1 (MQ=255) gcggTGCTGACGCAACCGACGTGGGCGATGAGTTTAATCTTACCTTTCGCCTCTTCGGCGACGATTTc < 1:1642384/68‑1 (MQ=255) gcggTGCTGACGCAACCGACGTGGGCGATGAGTTTAATCTTACCTTTCGCCTCTTCGGCGACGATTTc < 1:2827792/68‑1 (MQ=255) gcggTGCTGACGCAACCGACGTGGGCGATGAGTTTAATCTTACCTTTCGCCTCTTCGGCGACGATTTc < 1:348936/68‑1 (MQ=255) gcggTGCTGACGCAACCGACGTGGGCGATGAGTTTAATCTTACCTTTCGCCTCTTCGGCGACGATTTc < 1:1281838/68‑1 (MQ=255) gcggTGCTGACGCAACCGACGTGGGCGATGAGTTTAATCTTACCTTTCGCCTCTTCGGCGACGATTTc < 1:677120/68‑1 (MQ=255) gcggTGCTGACGCAACCGACGTGGGCGATGAGTTTAATCTTACCTTTCGCCTCTTCGGCGACGATTTc < 1:2411595/68‑1 (MQ=255) gcggTGCTGACGCAACCGACGTGGGCGATGAGTTTAATCTTACCTTTCGCCTCTTCGGCGACGATTTc < 1:2474245/68‑1 (MQ=255) cggTGCTGACGCAACCGACGTGGGCGATGAGTTTAATCTTACCTTTCGCCTCTTCGGCGACGATTTcc < 1:1508592/68‑1 (MQ=255) cggTGCTGACGCAACCGACGTGGGCGATGAGTTTAATCTTACCTTTCGCCTCTTCGGCGACGATTTc < 1:3309827/67‑1 (MQ=255) cggTGCTGACGCAACCGACGTGGGCGATGAGTTTAATCTTACCTTTCGCCTCTTCGGCGACGATTTc < 1:781889/67‑1 (MQ=255) cggTGCTGACGCAACCGACGTGGGCGATGAGTTTAATCTTACCTTTCGCCTCTTCAGCGACGAtt < 1:1795718/65‑1 (MQ=255) cggTGCTGACCCAACCGACGTGGGCGATGAGTTTAATCTTACCTTTCGCCTCTTCGGCGACGATTTc < 1:3373227/67‑1 (MQ=255) cTGACGCAACCGACGTGGTCGATGAGTTTAATCTTACCTTTCGCCTCTTCAGCGACTAtt < 1:783615/60‑1 (MQ=255) gACGCAACCGACGTGGGCGATGAGTTTAATCTTACCTTTCGCCTCTTCGGCGACGATTTCCAGTACCTg > 1:2009726/1‑69 (MQ=255) cGCAACCGACGTGGGCGATGAGTTTAATCTTACCTTTCGCCTCTTCGGCGACGATTTc < 1:2717127/58‑1 (MQ=255) gCAACCGCCGTGGGCGATGAGTTTAATCTTACCTTTCGCCTCTTCAGCGACGATTTCCAGTTCCTGtt > 1:2510672/1‑68 (MQ=255) gCAACCGACGTGGGCGATGAGTTTAATCTTACCTTTCGCCTCTTCGGCGACGATTTCCAGTACCTGtt < 1:93718/68‑1 (MQ=255) gCAACCGACGTGGGCGATGAGTTTAATCTTACCTTTCGCCTCTTCGGCGACGATTTCCAGTACCTGtt < 1:2557303/68‑1 (MQ=255) gCAACCGACGTGGGCGATGAGTTTAATCTTACCTTTCGCCTCTTCAGCGACGATTTCCAGTACCTGTTc > 1:2679150/1‑69 (MQ=255) gCAACCGACGTGGGCGATGAGTTTAATCTTACCTTTCGCCTCTTCAGCGACGATTTCCAGTACCTGTTc > 1:1378441/1‑69 (MQ=255) gCAACCGACGTGGGCGATGAGTTTAATCTTACCTTTCGCCTCTTCAGCGACGATTTCCAGTACCTGTTc > 1:823378/1‑69 (MQ=255) gCAACCGACGTGGGCGATGAGTTTAATCTTACCTTTCGCCTCTTCAGCGACGATTTCCAGTACCTGTTc > 1:526339/1‑69 (MQ=255) gCAACCGACGTGGGCGATGAGTTTAATCTTACCTTTCGCCTCTTCAGCGACGATTTCCAGTACCTGTTc > 1:3396485/1‑69 (MQ=255) gCAACCGACGTGGGCGATGAGTTTAATCTTACCTTTCGCCTCTTCAGCGACGATTTCCAGTACCTGTTc > 1:3327143/1‑69 (MQ=255) gCAACCGACGTGGGCGATGAGTTTAATCTTACCTTTCGCCTCTTCAGCGACGATTTCCAGTACCTGTTc > 1:1863601/1‑69 (MQ=255) gCAACCGACGTGGGCGATGAGTTTAATCTTACCTTTCGCCTCTTCAGCGACGATTTCCAGTACCTGTTc > 1:3313399/1‑69 (MQ=255) gCAACCGACGTGGGCGATGAGTTTAATCTTACCTTTCGCCTCTTCAGCGACGATTTCCAGTACCTGTTc > 1:2169171/1‑69 (MQ=255) gCAACCGACGTGGGCGATGAGTTTAATCTTACCTTTCGCCTCTTCAGCGACGATTTCCAGTACCTGTTc > 1:2623357/1‑69 (MQ=255) gCAACCGACGTGGGCGATGAGTTTAATCTTACCTTTCGCCTCTTCAGCGACGATTTCCAGTACCTGTTc > 1:2991773/1‑69 (MQ=255) gCAACCGACGTGGGCGATGAGTTTAATCTTACCTTTCGCCTCTTCAGCGACGATTTCCAGTACCTGTTc > 1:3061237/1‑69 (MQ=255) gCAACCGACGTGGGCGATGAGTTTAATCTTACCTTTCGCCTCTTCAGCGACGATTTCCAGTACCTGTTc > 1:2282637/1‑69 (MQ=255) gCAACCGACGTGGGCGATGAGTTTAATCTTACCTTTCGCCTCTTCAGCGACGAGTTCCAGTACCTGTTc > 1:1817876/1‑69 (MQ=255) gCAACCGACGTGGGCGATGAGTATAATCTTACCTTTCGCCTCTTCAGCGACGATTTCCAGTACCTGTTc > 1:245053/1‑69 (MQ=255) aaCCGACGTGGGCGATGAGTTTAATCTTACCTTTCGCCTCTTCAGCGACGAtt < 1:476505/53‑1 (MQ=255) cGACGTGGGCGATGAGTTTAATCTTACCTTTCGCCTCTTCAGCGACGAtt < 1:1798140/50‑1 (MQ=255) ggCGATGAGTTTAATCTTACCTTTCGCCTCTTCGGCGACGATTTCCAGTACCTGTTCACGCTCGGAAAg > 1:2140372/1‑69 (MQ=255) ggCGATGAGTTTAATCTTACCTTTCGCCTCTTCGGCGACGATTTCCAGTACCTGTTCACGCTCGGAAAg > 1:2165622/1‑69 (MQ=255) gATGAGTTTAATCTTACCTTTCGCCTCTTCAGCGACGATTTCCAGTACCTGtt < 1:217779/53‑1 (MQ=255) gATGAGTTTAATCTTACCTTTCGCCTCTTCAGCGACGATTTCCAGTACCTGtt < 1:3302097/53‑1 (MQ=255) gATGAGTTTAATCTTACCTTTCGCCTCTTCAGCGACGATTTCCAGTACCTGtt < 1:2699268/53‑1 (MQ=255) gATGAGTTTAATCTTACCTTTCGCCTCTTCAGCGACGATTTCCAGTACCTGtt < 1:885662/53‑1 (MQ=255) gATGAGTTTAATCTTACCTTTCGCCTCTTCAGCGACGATTTCCAGTACCTGtt < 1:1411548/53‑1 (MQ=255) gATGAGTTTAATCTTACCTTTCGCCTCTTCAGCGACGATTTCCAGTACCTGtt < 1:1006278/53‑1 (MQ=255) gATGAGTTTAATCTTACCTTTCGCCTCTTCAGCGACGATTTCCAGTACCTGtt < 1:1623597/53‑1 (MQ=255) gATGAGTTTAATCTTACCTTTCGCCTCTTCAGCGACGATTTCCAGTACCTGtt < 1:1643006/53‑1 (MQ=255) gATGAGTTTAATCTTACCTTTCGCCTCTTCAGCGACGATTTCCAGTACCTGtt < 1:703397/53‑1 (MQ=255) gATGAGTTTAATCTTACCTTTCGCCTCTTCAGCGACGATTTCCAGTACCTGtt < 1:563517/53‑1 (MQ=255) gATGAGTTTAATCTTACCTTTCGCCTCTTCAGCGACGATTTCCAGTACCTGtt < 1:358011/53‑1 (MQ=255) gATGAGTTTAATCTTACCTTTCGCCTCTTCAGCGACGATTTCCAGTACCTGtt < 1:2285036/53‑1 (MQ=255) gATGAGTTTAATCTTACCTTTCGCCTCTTCAGCGACGATTTCCAGTACCTGtt < 1:1843936/53‑1 (MQ=255) gATGAGTTTAATCTTACCTTTCGCCTCTTCAGCGACGATTTCCAGTACCTGtt < 1:3361673/53‑1 (MQ=255) gATGAGTTTAATCTTACCTTTCGCCTCTTCAGCGACGATTTCCAGTACCTGtt < 1:2151340/53‑1 (MQ=255) gATGAGTTTAATCTTACCTTTCGCCTCTTCAGCGACGATTTCCAGTACCTGtt < 1:2959642/53‑1 (MQ=255) gATGAGTTTAATCTTACCTTTCGCCTCTTCAGCGACGATTTCCAGTACCTGtt < 1:3325390/53‑1 (MQ=255) gATGAGTTTAATCTTACCTTTCGCCTCTTCAGCGACCATTTCCAGTACCTGtt < 1:64295/53‑1 (MQ=39) gATGACTTTAATCTTACCTTTCGCCTCTTCAGCGACGATTTCCAGTACCTGtt < 1:2140391/53‑1 (MQ=39) tAATCTTACCTTTCGCCTCTTCGGCGACGATTTCCAGTACCTGTTCACGCTCGGAAAGGCTTTGTACaa > 1:2258280/1‑69 (MQ=255) | GGCTTTCGGCGGTGCTGACGCAACCGACGTGGGCGATGAGTTTAATCTTACCTTTCGCCTCTTCGGCGACGATTTCCAGTACCTGTTCACGCTCGGAAAGGCTTTGTACAA > minE/2188594‑2188704 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |