| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,188,733 | T→C | 46.2% | L42L (TTA→TTG) | nanA ← | N‑acetylneuraminate lyase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,188,733 | 0 | T | C | 46.2% | 0.1 / 30.4 | 39 | L42L (TTA→TTG) | nanA | N‑acetylneuraminate lyase |
| Reads supporting (aligned to +/- strand): ref base T (9/12); new base C (9/9); total (18/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.52e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01 | |||||||||||
TACCTGTTCACGCTCGGAAAGGCTTTGTACAAAGGCCTCGCCGGTCGAACCACCCACGTATAAACCGTCGATGCCCTGCTGAATATTGAACTGAACCAGGCGACGCAGACTCGCTTTATCCAGTGCT > minE/2188673‑2188799 | tACCTGTTCACGCTCGGAAAGGCTTTGTACAAAGGCCTCGCCGGTCGAACCACCCACGTATAAACCGta < 1:1064843/69‑2 (MQ=255)tACCTGTTCACGCTCGGAAAGGCTTTGTACAAAGGCCTCGCCGGTCGAACCACCCACGTATAAACCGTc < 1:1570276/69‑1 (MQ=255) cACGCTCGGAAAGGCTTTGTACAAAGGCCTCGCCGGTCGAACCACGCACGTATAAACCGTCGATGccc < 1:197291/68‑1 (MQ=255) cACGCTCGGAAAGGCTTTGTACAAAGGCCTCGCCGGTCGAACCACCCACGTATAAACCGTCGATGccc < 1:1961253/68‑1 (MQ=255) cACGCTCGGAAAGGCTTTGTACAAAGGCCTCGCCGGTCGAACCACCCACGTATAAACCGTCGATGccc < 1:3387605/68‑1 (MQ=255) cACGCTCGGAAAGGCTTTGTACAAAGGCCTCGCCGGTCGAACCACCCACGTATAAACCGTCGATGccc < 1:2564508/68‑1 (MQ=255) cACGCTCGGAAAGGCTTTGTACAAAGGCCTCGCCGGTCGAACCACCCACGTATAAACCGTCGATGccc < 1:656908/68‑1 (MQ=255) cACGCTCGGAAAGGCTTTGTACAAAGGCCTCGCCGGTCGAACCACCCACGTATAAACCGTCGATGccc < 1:1954099/68‑1 (MQ=255) cACGCTCGGAAAGGCTTTGTACAAAGGCCTCGCCGGTCGAACCACCCACGTATAAACCGTCGATGccc < 1:1869254/68‑1 (MQ=255) cACGCTCGGAAAGGCTTTGTACAAAGGCCTCGCCGGTCGAACCACCCACGTATAAACCGTCGATGccc < 1:1761690/68‑1 (MQ=255) cACGCTCGGAAAGGCTTTGTACAAAGGCCTCGCCGGTCGAACCACCCACGTATAAACCGTCGATGccc < 1:1664990/68‑1 (MQ=255) cACGCTCGGAAAGGCTTTGTACAAAGGCCTCGCCGGTCGAACCACCCACGTATAAACCGTCGATGccc < 1:1374054/68‑1 (MQ=255) cACGCTCGGAAAGGCTTTGTACAAAGGCCTCGCCGGTCGAACCACCCACGTATAAACCGTCGATGCCct > 1:1932953/1‑69 (MQ=255) cACGCTCGGAAAGGCTTTGTACAAAGGCCTCGCCGGTCGAACCACCCACGTATAAACCGTCGATGCCct > 1:1940893/1‑69 (MQ=255) cACGCTCGGAAAGGCTTTGTACAAAGGCCTCGCCGGTCGAACCACCCACGTATAAACCGTCGATGCCct > 1:2775173/1‑69 (MQ=255) cACGCTCGGAAAGGCTTTGTACAAAGGCCTCGCCGGTCGAACCACCCACGTATAAACCGTCGATGCCct > 1:2739998/1‑69 (MQ=255) gCTCTGAAAGGCTCTGTACAAAGGCTTCGCCTGTCGAACCCCCCACGTACAAACCGTCGATGCCctgct < 1:3110317/69‑1 (MQ=255) gCTCTGAAAGGCTCTGTACAAAGGCTTCGCCTGTCGAACCCCCCACGTACAAACCGTCGATGCCctgct < 1:1769255/69‑1 (MQ=255) gCTCTGAAAGGCTCTGTACAAAGGCTTCGCCTGTCGAACCCCCCACGTACAAACCGTCGATGCCctgct < 1:2860584/69‑1 (MQ=255) gCTCTGAAAGGCTCTGTACAAAGGCTTCGCCTGTCGAACCCCCCACGTACAAACCGTCGATGCCctgct < 1:1133991/69‑1 (MQ=255) gCTCTGAAAGGCTCTGTACAAAGGCTTCGCCTGTCGAACCCCCCACGTACAAACCGTCGATGCCctgct < 1:2319035/69‑1 (MQ=255) gCTCTGAAAGGCTCTGTACAAAGGCTTCGCCTGTCGAACCCCCCACGTACAAACCGTCGATGCCctgct < 1:1267334/69‑1 (MQ=255) gCTCTGAAAGGCTCTGTACAAAGGCTTCGCCTGTCGAACCCCCCACGTACAAACCGTCGATGCCctgct < 1:2070608/69‑1 (MQ=255) gCTCTGAAAGGCTCTGTACAAAGGCTTCGCCTGTCGAACCCCCCACGTACAAACCGTCGATGCCctgct < 1:206424/69‑1 (MQ=255) gCTCTGAAAGGCTCTGTACAAAGGCTTCGCCTGTCGAACCCCCCACGTACAAACCGTCGATGCCctgct < 1:1055692/69‑1 (MQ=255) ggCTTTGTACAAAGGCATCGCCGGTCGAACCACCCACGTATAAACCGTCGATg > 1:2659932/1‑53 (MQ=255) cAAAGGCCTCGCCGGTCGAACCACCCACGTATAAACCGTCGATGCCCTGCTGaa > 1:1127828/1‑54 (MQ=255) cAAAGGCCTCGCCGGTCGAACCACCCACGTATAAACCGTCGATGCCCTGCTGaa > 1:3007947/1‑54 (MQ=255) cAAAGGCCTCGCCGGTCGAACCACCCACGTATAAACCGTCGATGCCCTGCTGaa > 1:3343028/1‑54 (MQ=255) cAAAGGCCTCGCCGGTCGAACCACACACGTATAAACCGTCGATGCCCTGCTGa > 1:2600904/1‑53 (MQ=255) ccacGTACAAACCGTCGATGCCCTGCTGAATATTGAACTGAACCAGGCGGCGCAGa > 1:3404817/1‑56 (MQ=255) ccacGTACAAACCGTCGATGCCCTGCTGAATATTGAACTGAACCAGGCGGCGCAGa > 1:789998/1‑56 (MQ=255) ccacGTACAAACCGTCGATGCCCTGCTGAATATTGAACTGAACCAGGCGGCGCAGa > 1:756946/1‑56 (MQ=255) ccacGTACAAACCGTCGATGCCCTGCTGAATATTGAACTGAACCAGGCGGCGCAGa > 1:3083644/1‑56 (MQ=255) ccacGTACAAACCGTCGATGCCCTGCTGAATATTGAACTGAACCAGGCGGCGCAGa > 1:2870645/1‑56 (MQ=255) ccacGTACAAACCGTCGATGCCCTGCTGAATATTGAACTGAACCAGGCGGCGCAGa > 1:2366887/1‑56 (MQ=255) ccacGTACAAACCGTCGATGCCCTGCTGAATATTGAACTGAACCAGGCGGCGCAGa > 1:2132783/1‑56 (MQ=255) ccacGTACAAACCGTCGATGCCCTGCTGAATATTGAACTGAACCAGGCGGCGCAGa > 1:1911746/1‑56 (MQ=255) ccacGTACAAACCGTCGATGCCCTGCTGAATATTGAACTGAACCAGGCGGCGCAGa > 1:189022/1‑56 (MQ=255) tataAACCGTCGATGCCCTGCTGAATATTGAACTGAACCAGGCGACGTAGACTCGCTTTATCCAGTGCt > 1:692610/1‑69 (MQ=255) tataAACCGTCGATGCCCTGCTGAATATTGAACTGAACCAGGCGACGCAGACTCGCTTTATCCAGTGCt > 1:527941/1‑69 (MQ=255) | TACCTGTTCACGCTCGGAAAGGCTTTGTACAAAGGCCTCGCCGGTCGAACCACCCACGTATAAACCGTCGATGCCCTGCTGAATATTGAACTGAACCAGGCGACGCAGACTCGCTTTATCCAGTGCT > minE/2188673‑2188799 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |