| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,189,346 | A→G | 32.3% | H142H (CAT→CAC) | nanR ← | DNA‑binding transcriptional dual regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,189,346 | 0 | A | G | 32.3% | 87.8 / 57.3 | 108 | H142H (CAT→CAC) | nanR | DNA‑binding transcriptional dual regulator |
| Reads supporting (aligned to +/- strand): ref base A (20/53); new base G (16/19); total (36/72) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.08e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01 | |||||||||||
Only 100 of 109 total aligned reads displayed.
GTTATCCAGCGACTGACTGTTGATTTCCAGTGCTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGTAATTGTTCGAAATGGGCAAT > minE/2189280‑2189411 | gttATCCAGCGACTGACTGTTGATTTCCAGTGCTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCGTg < 1:1004281/69‑1 (MQ=255)gttATCCAGCGACTGACTGTTGATTTCCAGTGCTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCGTg < 1:2121537/69‑1 (MQ=255)gttATCCAGCGACTGACTGTTGATTTCCAGTGCTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCGTg < 1:2621566/69‑1 (MQ=255)gttATCCAGCGACTGACTGTTGATTTCCAGTGCTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCGTg < 1:2636421/69‑1 (MQ=255)gttATCCAGCGACTGACTGTTGATTTCCAGTGCTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCGTg < 1:2786914/69‑1 (MQ=255)gttATCCAGCGACTGACTGTTGATTTCCAGTGCTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCGTg < 1:1825374/69‑1 (MQ=255)gttATCCAGCGACTGACTGTTGATTTCCAGTGCTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCGTg < 1:3047510/69‑1 (MQ=255)gttATCCAGCGACTGACTGTTGATTTCCAGTGCTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCGTg < 1:340271/69‑1 (MQ=255)gttATCCAGCGACTGACTGTTGATTTCCAGTGCTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCGTg < 1:470169/69‑1 (MQ=255)gttATCCAGCGACTGACTGTTGATTTCCAGTGCTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCGTg < 1:609459/69‑1 (MQ=255)gttATCCAGCGACTGACTGTTGATTTCCAGTGCTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCGTg < 1:637746/69‑1 (MQ=255)gttATCCAGCGACTGACTGTTGATTTCCAGTGCTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCGTg < 1:1190956/69‑1 (MQ=255)gttATCCAGCGACTGACTGTTGATTTCCAGTGCTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCGTg < 1:827469/69‑1 (MQ=255) ttATCCAGCGACTGACTGTTGATTTCCAGTGCTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCGTg < 1:2071511/68‑1 (MQ=255) ttATCCAGCGACTGACTGTTGATTTCCAGTGCTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCGTg < 1:951301/68‑1 (MQ=255) cAGCGACTGACTGTTGATTTCCAGTGCTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGc > 1:263513/1‑69 (MQ=255) gCGACTGACTGTTGATTTCCAGTGCTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCg < 1:2554459/69‑1 (MQ=255) gactgTTGATTTCCAGTGCTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCGTg < 1:658129/55‑1 (MQ=255) tgTTGATTTCCAGTGCTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAgcgc < 1:1644689/68‑1 (MQ=255) tgTTGATTTCCAGTGCTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAgcgc < 1:1647682/68‑1 (MQ=255) gATTTCCAGTGCTTTTGCCAGCAAATCGATTTTCTCATCGGTGGCATGTTCAGCCg > 1:3286519/1‑56 (MQ=255) gATTTCCAGTGCTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGcc > 1:3100707/1‑55 (MQ=255) gATTTCCAGTGCTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCg > 1:701595/1‑56 (MQ=255) gATTTCCAGTGCTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCg > 1:2604447/1‑56 (MQ=255) gATTTCCAGTGCTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCg > 1:187915/1‑56 (MQ=255) gATTTCCAGTGCTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCg > 1:1877253/1‑56 (MQ=255) gATTTCCAGTGCTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCg > 1:790590/1‑56 (MQ=255) gATTTCCAGTGCTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCg > 1:2794065/1‑56 (MQ=255) gATTTCCAGTGCTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCg > 1:178439/1‑56 (MQ=255) gATTTCCAGTGCTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCg > 1:759928/1‑56 (MQ=255) gATTTCCAGTGCTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCg > 1:362378/1‑56 (MQ=255) gATTTCCAGTGCTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCg > 1:411399/1‑56 (MQ=255) gATTTCCAGTGCTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCg > 1:1280539/1‑56 (MQ=255) gATTTCCAGTGCTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCGCa > 1:1140108/1‑58 (MQ=255) gATTTCCAGTGCTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCa < 1:271570/68‑1 (MQ=255) gATTTCCAGTGCTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCa < 1:182316/68‑1 (MQ=255) gATTTCCAGTGCTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAg < 1:1431316/69‑1 (MQ=255) gATTTCCAGTGCTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAg < 1:2406824/69‑1 (MQ=255) gATTTCCAGTGCTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAg < 1:311113/69‑1 (MQ=255) gATTTCCAGTGCTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAg < 1:2759280/69‑1 (MQ=255) gATTTCCAGTGCTTTTGCCAGCAAATCGATTTGCTCAACGGTGGCGTGTTCAGCCGCATAGCGCACCa < 1:65945/68‑1 (MQ=255) ttCCAGTGCTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGAc > 1:156115/1‑68 (MQ=255) ttCCAGTGCTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGAc > 1:3183760/1‑68 (MQ=255) ttCCAGTGCTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGAc > 1:2042111/1‑68 (MQ=255) cAGTGCTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTgg < 1:2132654/68‑1 (MQ=255) cAGTGCTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTgg < 1:1465435/68‑1 (MQ=255) cTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCGTGTTCAGCCGCATAg > 1:89318/1‑50 (MQ=255) cTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCGTGTTCAGCCGCATAGCGCACCAGGCTGGa > 1:356733/1‑64 (MQ=255) cTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCGTGTTCAGCCGCATAGCGCACCAGGCTGGAt > 1:820929/1‑65 (MQ=255) cTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCGTGTTCAGCCGCATAGCGCACCAGGCTGGAt > 1:3410441/1‑65 (MQ=255) cTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCGTGTTCAGCCGCATAGCGCACCAGGCTGGAt > 1:981550/1‑65 (MQ=255) cTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCGTGTTCAGCCGCATAGCGCACCAGGCTGGAt > 1:364820/1‑65 (MQ=255) cTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCGTGTTCAGCCGCATAGCGCACCAGGCTGGAt > 1:2230445/1‑65 (MQ=255) cTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCGTGTTCAGCCGCATAGCGCACCAGGCTGGAt > 1:3171321/1‑65 (MQ=255) cTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCGTGTTCAGCCGCATAGCGCACCAGGCTGGAt > 1:3115246/1‑65 (MQ=255) cTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCGTGTTCAGCCGCATAGCGCACCAGGCTGGAt > 1:904576/1‑65 (MQ=255) cTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCGTGTTCAGCCGCATAGCGCACCAGGCTGGAt > 1:661873/1‑65 (MQ=255) cTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCGTGTTCAGCCGCATAGCGCACCAGGCTGGAt > 1:1831001/1‑65 (MQ=255) cTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCGTGTTCAGCCGCATAGCGCACCAGGCTGGAt > 1:869982/1‑65 (MQ=255) cTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCGTGTTCAGCCGCATAGCGCACCAGGCTGGAt > 1:1002669/1‑65 (MQ=255) tttGCCAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAg < 1:3124598/69‑1 (MQ=255) gCCAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAg < 1:2648101/66‑1 (MQ=255) gCCAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGaa < 1:2923435/68‑1 (MQ=255) gCCAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGaa < 1:2413819/68‑1 (MQ=255) gCCAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGaa < 1:2028037/68‑1 (MQ=255) gCCAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGaa < 1:755865/68‑1 (MQ=255) gCCAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGaa < 1:1226582/68‑1 (MQ=255) aGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAg < 1:2933674/68‑1 (MQ=255) aGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAg < 1:2929973/68‑1 (MQ=255) aaTCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGTa < 1:2140587/69‑1 (MQ=255) aaTCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGTa < 1:101216/69‑1 (MQ=255) aaTCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGTa < 1:1423674/69‑1 (MQ=255) aaTCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGTa < 1:755203/69‑1 (MQ=255) aaTCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGTa < 1:1407009/69‑1 (MQ=255) aaTCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGTa < 1:2224951/69‑1 (MQ=255) aaTCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGTa < 1:1767446/69‑1 (MQ=255) aaTCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGTa < 1:2005175/69‑1 (MQ=255) aaTCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGTa < 1:1993265/69‑1 (MQ=255) tCGATTTGCTCATCGGTGGCATGTTCAGGCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGTAAt < 1:1379771/69‑1 (MQ=255) tCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGTa < 1:1367082/67‑1 (MQ=255) tCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGTAAt < 1:21633/69‑1 (MQ=255) tCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGTAAt < 1:3332798/69‑1 (MQ=255) tCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGTAAt < 1:944748/69‑1 (MQ=255) tCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGTAAt < 1:2509234/69‑1 (MQ=255) tCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGTAAt < 1:2049839/69‑1 (MQ=255) tCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGTAAt < 1:1123279/69‑1 (MQ=255) tCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGTAAt < 1:1315033/69‑1 (MQ=255) tCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGTAAt < 1:1375831/69‑1 (MQ=255) tCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGTAAt < 1:3281477/69‑1 (MQ=255) tCGATTTGCTCATCGGTGGCATGCTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGTAAt < 1:767534/69‑1 (MQ=255) ggTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAg < 1:1568194/43‑1 (MQ=255) ggTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAg < 1:958954/43‑1 (MQ=255) ggTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGTAATTGTTCGAAATggg > 1:2722101/1‑68 (MQ=255) ggTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGTAATTGTTCGAAATggg > 1:1157186/1‑68 (MQ=255) ggTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGTAATTGTTCGAAATGGGc < 1:2738566/69‑1 (MQ=255) ggTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGTAATTGTTCGAAATGGGc < 1:753931/69‑1 (MQ=255) ggTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGTAATTGTTCGAAATGGGc < 1:2623732/69‑1 (MQ=255) ggTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGTAATTGTTCGAAATGGGc < 1:926496/69‑1 (MQ=255) ggTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGTAATTGTTCGAAATGGGc < 1:371443/69‑1 (MQ=255) ggCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGTAATTGTTCGAAATGGGCAAt < 1:303644/69‑1 (MQ=255) | GTTATCCAGCGACTGACTGTTGATTTCCAGTGCTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGTAATTGTTCGAAATGGGCAAT > minE/2189280‑2189411 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |