| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,196,732 | C→A | 32.4% | G236G (GGC→GGA) | degQ → | serine endoprotease, periplasmic |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,196,732 | 0 | C | A | 32.4% | 88.0 / 46.4 | 74 | G236G (GGC→GGA) | degQ | serine endoprotease, periplasmic |
| Reads supporting (aligned to +/- strand): ref base C (29/21); new base A (13/11); total (42/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.05e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.96e-01 | |||||||||||
CACTATTAAACCTTAACGGTGAGTTAATTGGCATCAACACTGCAATCCTTGCGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAGTAATATGGCGCGAACACTGG > minE/2196674‑2196787 | cACTATTAAACCTTAACGGTGAGTTAATTGGCATCAACACTGCAATCCTTGCGCCTGGCGGCGGGAGc > 1:2567509/1‑68 (MQ=255) ttAAACCTTAACGGTGAGTTAATTGGCATCAACACTGCAATCCTTGCGCCTGGAGGCGGTAGCGTCggg > 1:2386400/1‑69 (MQ=255) ttAAACCTTAACGGTGAGTTAATTGGCATCAACACTGCAATCCTTGCGCCTGGAGGCGGTAGCGTCggg > 1:250226/1‑69 (MQ=255) ttAAACCTTAACGGTGAGTTAATTGGCATCAACACTGCAATCCTTGCGCCTGGAGGCGGTAGCGTCggg > 1:781797/1‑69 (MQ=255) ttAAACCTTAACGGTGAGTTAATTGGCATCAACACTGCAATCCTTGCGCCTGGAGGCGGTAGCGTCggg > 1:548455/1‑69 (MQ=255) ttAAACCTTAACGGTGAGTTAATTGGCATCAACACTGCAATCCTTGCGCCTGGAGGCGGTAGCGTCggg > 1:2064589/1‑69 (MQ=255) ttAAACCTTAACGGTGAGTTAATTGGCATCAACACTGCAATCCTTGCGCCTGGAGGCGGTAGCGTCggg > 1:2821365/1‑69 (MQ=255) ttAAACCTTAACGGTGAGTTAATTGGCATCAACACTGCAATCCTTGCGCCTGGAGGCGGTAGCGTCggg > 1:3202322/1‑69 (MQ=255) ttAAACCTTAACGGTGAGTTAATTGGCATCAACACTGCAATCCTTGCGCCTGGAGGCGGTAGCGTCggg > 1:2022614/1‑69 (MQ=255) ttAAACCTTAACGGTGAGTTAATTGGCATCAACACTGCAATCCTTGCGCCTGGAGGCGGTAGCGTCggg > 1:2873612/1‑69 (MQ=255) ttAAACCTTAACGGTGAGTTAATTGGCATCAACACTGCAATCCTTGCGCCTGGAGGCGGTAGCGTCggg > 1:1620094/1‑69 (MQ=255) ttAAACCTTAACGGTGAGTTAATTGGCATCAACACTGCAATCCTTGCGCCTGGAGGCGGTAGCGTCggg > 1:3091647/1‑69 (MQ=255) ttAAACCTTAACGGTGAGTTAATTGGCATCAACACTGCAATCCTTGCGCCTGGAGGCGGTAGCGTCggg > 1:1711737/1‑69 (MQ=255) ttAAACCTTAACGGTGAGTTAATTGGCATCAACACTGCAATCCTTGCGCCTGGAGGCGGTAGCGTCggg > 1:1744775/1‑69 (MQ=255) ccTTAACGGTGAGTTAATTGGCATCAACACTGCAATCCTTGCGCCTGGCGGCGGGAGCGTCGggattgg > 1:2288996/1‑69 (MQ=255) tAACGGTGAGTTAATTGGCATCAACACTGCAATCCTTGCGCCTGGCGGCGGGAGCGTCGggattggatt > 1:2908533/1‑69 (MQ=255) tAACGGTGAGTTAATTGGCATCAACACTGCAATCCTTGCGCCTGGCGGCGGGAGCGTCGggattggat > 1:1371695/1‑68 (MQ=255) tAACGGTGAGTTAATTGGCAGCAACACTGCAATCCTTGCGCCTGGCGGCGGGAGCGTCGggattggatt > 1:3052392/1‑69 (MQ=255) cGGTGAGTTAATTGGCATCAACACTGCAATCCTTGCGCCTGGCGGCGGGAGCGTCGGGATTGGAttt < 1:501677/67‑1 (MQ=255) ggTGAGTTAATTGGCATCAACACTGCAATCCTTGCGCCTGGCGGCGGGAGCGTCGGGATTGGAttt < 1:1353307/66‑1 (MQ=255) ggTGAGTTAATTGGCATCAACACTGCAATCCTTGCGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGcc < 1:3128764/69‑1 (MQ=255) ggTGAGTTAATTGGCATCAACACTGCAATCCTTGCGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGcc < 1:1395610/69‑1 (MQ=255) ggTGAGTTAATTGGCATCAACACTGCAATCCTTGCGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGcc < 1:1358447/69‑1 (MQ=255) ggTGAGTTAATTGGCATCAACACTGCAATCCTTGCGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGcc < 1:1236261/69‑1 (MQ=255) ggTGAGTTAATTGGCATCAACACTGCAATCCTTGCGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGcc < 1:2353887/69‑1 (MQ=255) ggTGAGTTAATTGGCATCAACACTGCAATCCTTGAGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGcc < 1:238179/69‑1 (MQ=255) gAGTTAATTGGCATCAACACTGCAATCCTTGCGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGCCATc < 1:857958/69‑1 (MQ=255) gAGTTAATTGGCATCAACACTGCAATCCTTGCGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGCCATc < 1:2582798/69‑1 (MQ=255) gAGTTAATTGGCATCAACACTGCAATCCTTGCGCCTGGAGGCGGTAGCGTCGGGATTGGATTTGCCAtt < 1:2233128/69‑2 (MQ=255) gAGTTAATTGGCATCAACACTGCAATCCTTGCGCCTGGAGGCGGTAGCGTCGGGATTGGATTTGCCAtt < 1:2173058/69‑2 (MQ=255) gAGTTAATTGGCATCAACACTGCAATCCTTGCGCCTGGAGGCGGTAGCGTCGGGATTGGATTTGCCAtt < 1:1863498/69‑2 (MQ=255) gAGTTAATTGGCATCAACACTGCAATCCTTGCGCCTGGAGGCGGTAGCGTCGGGATTGGATTTGCCAtt < 1:2448924/69‑2 (MQ=255) gAGTTAATTGGCATCAACACTGCAATCCTTGCGCCTGGAGGCGGTAGCGTCGGGATTGGATTTGCCAtt < 1:2561375/69‑2 (MQ=255) gAGTTAATTGGCATCAACACTGCAATCCTTGCGCCTGGAGGCGGTAGCGTCGGGATTGGATTTGCCAtt < 1:1361101/69‑2 (MQ=255) gAGTTAATTGGCATCAACACTGCAATCCTTGCGCCTGGAGGCGGTAGCGTCGGGATTGGATTTGCCAtt < 1:332380/69‑2 (MQ=255) gAGTTAATTGGCATCAACACTGCAATCCTTGCGCCTGGAGGCGGTAGCGTCGGGATTGGATTTGCCAtt < 1:2921845/69‑2 (MQ=255) gAGTTAATTGGCATCAACACTGCAATCCTTGCGCCTGGAGGCGGTAGCGTCGGGATTGGATTTGCCAtt < 1:2791998/69‑2 (MQ=255) gAGTTAATTGGCATCAACACTGCAATCCTTGCGCCTGGAGGCGGTAGCGTCGGGATTGGATTTGCCAtt < 1:202096/69‑2 (MQ=255) gAGTTAATTGGCATCAACACTGCAATCCTTGCGCCTGGAGGCGGTAGCGTCGGGATTGGATTTGCCAtt < 1:3297913/69‑2 (MQ=255) tGGCATCAACACTGCAATCCTTGCGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAGTaa < 1:3195417/69‑1 (MQ=255) tGGCATCAACACTGCAATCCTTGCGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAGTaa < 1:653968/69‑1 (MQ=255) tGGCATCAACACTGCAATCCTTGCGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAGTaa < 1:2588073/69‑1 (MQ=255) tGGCATCAACACTGCAATCCTTGCGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAGTaa < 1:203164/69‑1 (MQ=255) aaCACTGCAATCCTTGCGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGc > 1:1711120/1‑50 (MQ=255) aaCACTGCAATCCTTGCGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGc > 1:289846/1‑50 (MQ=255) aaCACTGCAATCCTTGCGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGc > 1:2770656/1‑50 (MQ=255) aaCACTGCAATCCTTGCGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGc > 1:802172/1‑50 (MQ=255) aaCACTGCAATCCTTGCGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGc > 1:80237/1‑50 (MQ=255) aaCACTGCAATCCTTGCGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAGTAATATg > 1:3416800/1‑66 (MQ=255) aaCACTGCAATCCTTGCGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAGTAATATg > 1:3186949/1‑66 (MQ=255) aaCACTGCAATCCTTGCGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAGTAATATGgc > 1:2207167/1‑68 (MQ=255) aaCACTGCAATCCTTGCGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAGTAATATGgc > 1:2955960/1‑68 (MQ=255) aaCACTGCAATCCTTGCGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAGTAATATGgc > 1:1259986/1‑68 (MQ=255) aaCACTGCAATCCTTGCGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAGTAATATGgc > 1:3359550/1‑68 (MQ=255) aaCACTGCAATCCTTGCGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAGTAATATGgc > 1:2140669/1‑68 (MQ=255) aaCACTGCAATCCTTGCGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAGTAATATGgc > 1:376986/1‑68 (MQ=255) aaCACTGCAATCCTTGCGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAGTAATATGgc > 1:384932/1‑68 (MQ=255) aaCACTGCAATCCTTGCGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAGTAATATGgc > 1:459812/1‑68 (MQ=255) aaCACTGCAATCCTTGCGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAGTAATATGgc > 1:921555/1‑68 (MQ=255) aaCACTGCAATCCTTGCGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAGTAATATGgc > 1:1329867/1‑68 (MQ=255) cTGCAATCCTTGCGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAGTAATATGGCGCGaa < 1:1094063/69‑1 (MQ=255) aTCCTTTCGCCTGGCGGCGGGAGCGTCGGGATTGGAttt < 1:2152894/39‑1 (MQ=255) cTTGCGCCTGGCGGCGTGAGCGTCGGGATTGGATTTGCCATCCCCAGTAATATGGcgcg < 1:180876/59‑1 (MQ=255) cTTGCGCCTGGCGGCGGGCGCGTCGGGATTGGAttt > 1:234864/1‑36 (MQ=255) cTTGCGCCTGGCGGCGGGAGCGTCGGGATTGGATTTg > 1:2430140/1‑37 (MQ=255) cTTGCGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAGTAATATGGcgcg < 1:744283/59‑1 (MQ=255) cTTGCGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAGTAATATGGcgcg < 1:3296262/59‑1 (MQ=255) cTTGCGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAGTAATATGGcgcg < 1:818483/59‑1 (MQ=255) cTTGCGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAGTAATATGGcgcg < 1:854298/59‑1 (MQ=255) cTTGCGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAGTAATATGGCGCGGACACTgg > 1:3120671/1‑67 (MQ=255) cTTGCGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAGTAATATGGCGCGAACACTgg > 1:9778/1‑67 (MQ=255) cTTGCGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAGTAATATGGCGCGAACACTgg > 1:1282674/1‑67 (MQ=255) cTTGCGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAGTAATATGGCGCGAACACTgg > 1:1347769/1‑67 (MQ=255) cTTGCGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAGTAATATGGCGCGAACACTgg > 1:1870436/1‑67 (MQ=255) | CACTATTAAACCTTAACGGTGAGTTAATTGGCATCAACACTGCAATCCTTGCGCCTGGCGGCGGGAGCGTCGGGATTGGATTTGCCATCCCCAGTAATATGGCGCGAACACTGG > minE/2196674‑2196787 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |