| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,198,000 | T→C | 40.5% | I173I (ATT→ATC) | degS → | serine endoprotease, periplasmic |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,198,000 | 0 | T | C | 40.5% | 27.1 / 52.2 | 74 | I173I (ATT→ATC) | degS | serine endoprotease, periplasmic |
| Reads supporting (aligned to +/- strand): ref base T (20/24); new base C (24/6); total (44/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.80e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.24e-01 | |||||||||||
Only 100 of 111 total aligned reads displayed.
ATTGGCGACGTAGTACTGGCGATCGGTAACCCGTACAACCTCGGGCAGACCATTACCCAGGGGATTATTAGTGCCACGGGTCGAATCGGTCTGAACCCGACCGGGCGGCAAAACTTCCTCCAAACCGATGCTTC > minE/2197932‑2198065 | aTTGGCGACGTAGTACTGGCGATCGGTAACCCGTACAACCTCGGGCAGACCATTACCCAGGGTattatt < 1:179/69‑1 (MQ=255)aTTGGCGACGTAGTACTGGCGATCGGTAACCCGTACAACCTCGGGCAGACCATTACCCAGGGGattatt < 1:1966793/69‑1 (MQ=255)aTTGGCGACGTAGTACTGGCGATCGGTAACCCGTACAACCTCGGGCAGACCATTACCCAGGGGattatt < 1:2382658/69‑1 (MQ=255)aTTGGCGACGTAGTACTGGCGATCGGTAACCCGTACAACCTCGGGCAGACCATTACCCAGGGGattatt < 1:1253792/69‑1 (MQ=255)aTTGGCGACGTAGTACTGGCGATCGGTAACCCGTACAACCTCGGGCAGACCATTACCCAGGGGattatt < 1:834280/69‑1 (MQ=255)aTTGGCGACGTAGTACTGGCGATCGGTAACCCGTACAACCTCGGGCAGACCATTACCCAGGGGattatt < 1:174706/69‑1 (MQ=255) ggCGACGTAGTACTGGCGATCGGTAACCCGTACAACCTCGGGCAGACCATTACCCAGGGGATTATTAGt > 1:1535400/1‑69 (MQ=255) ggCGACGTAGTACTGGCGATCGGTAACCCGTACAACCTCGGGCAGACCATTACCCAGGGGATTATTAGt > 1:1110421/1‑69 (MQ=255) ggCGACGTAGTACTGGCGATCGGTAACCCGTACAACCTCGGGCAGACCATTACCCAGGGGATTATTAGt > 1:73120/1‑69 (MQ=255) cTGGCGATCGGTAACCCGTACAACCTCGGGCAGACCATTACCCAGGGGattatt < 1:3242785/54‑1 (MQ=255) cTGGCGATCGGTAACCCGTACAACCTCGGGCAGACCATTACCCAGGGGattatt < 1:1473397/54‑1 (MQ=255) cTGGCGATCGGTAACCCGTACAACCTCGGGCAGACCATTACCCAGGGGattatt < 1:3112975/54‑1 (MQ=255) cTGGCGATCGGTAACCCGTACAACCTCGGGCAGACCATTACCCAGGGGATTATTAGTGCCACGGGTCGa > 1:1643532/1‑69 (MQ=255) cTGGCGATCGGTAACCCGTACAACCTCGGGCAGACCATTACCCAGGGGATTATTAGTGCCACGGGTCGa > 1:673289/1‑69 (MQ=255) cTGGCGATCGGTAACCCGTACAACCTCGGGCAGACCATTACCCAGGGGATTATTAGTGCCACGGGTCGa > 1:2514891/1‑69 (MQ=255) cTGGCGATCGGTAA‑CCGTACAACCTCGGGCAGACCATTACCCAGGGGattatt < 1:1427077/53‑1 (MQ=255) ggCGATCGGTAACCCGTACAACCTCGGGCAGACCATTACCCAGGGGattatt < 1:1895266/52‑1 (MQ=255) ggCGATCGGTAACCCGTACAACCTCGGGCAGACCATTACCCAGGGGattatt < 1:2515452/52‑1 (MQ=255) cGGTAACCCGTACAACCTCGGGCAGACCATTACCCAGGGGattatt < 1:1841831/46‑1 (MQ=255) cGGTAACCCGTACAACCTCGGGCAGACCATTACCCAGGGGattatt < 1:1793516/46‑1 (MQ=255) cGGTAACCCGTACAACCTCGGGCAGACCATTACCCAGGGGattatt < 1:3079592/46‑1 (MQ=255) cGGTAACCCGTACAACCTCGGGCAGACCATTACCCAGGGGattatt < 1:2006425/46‑1 (MQ=255) cGGTAACCCGTACAACCTCGGGCAGACCATTACCCAGGGGattatt < 1:25539/46‑1 (MQ=255) cGGTAACCCGTACAACCTCGGGCAGACCATTACCCAGGGGattatt < 1:17404/46‑1 (MQ=255) cGGTAACCCGTACAACCTCGGGCAGACCATTACCCAGGGGattatt < 1:379174/46‑1 (MQ=255) cGGTAACCCGTACAACCTCGGGCAGACCATTACCCAGGGGattatt < 1:123319/46‑1 (MQ=255) cGGTAACCCGTACAACCTCGGGCAGACCATTACCCAGGGGattatt < 1:2538957/46‑1 (MQ=255) cGGTAACCCGTACAACCTCGGGCAGACCATTACCCAGGGGattatt < 1:1628720/46‑1 (MQ=255) cGGTAACCCGTACAACCTCGGGCAGACCATTACCCAGGGGattatt < 1:1620686/46‑1 (MQ=255) cGGTAACCCGTACAACCTCGGGCAGACCATTACCCAGGGGattatt < 1:3386204/46‑1 (MQ=255) cGGTAACCCGTACAACCTCGGGCAGACCATTACCCAGGGGattatt < 1:2326805/46‑1 (MQ=255) cGGTAACCCGTACAACCTCGGGCAGACCATTACCCAGGGGattatt < 1:735825/46‑1 (MQ=255) cGGTAACCCGTACAACCTCGGGCAGACCATTACCCAGGGGattatt < 1:3083626/46‑1 (MQ=255) cGGTAACCCGTACAACCTCGGGCAGACCATTACCCAGGGGattatt < 1:119330/46‑1 (MQ=255) cGGTAACCCGTACAACCTCGGGCAGACCATTACCCAGGGGATTATTAGTGCCACGGGTCGAATCGGTc > 1:272403/1‑68 (MQ=255) cGGTAACCCGTACAACCTCGGGCAGACCATTACCCAGGGGATTATTAGTGCCACGGGTCGAATCGGTc > 1:2970130/1‑68 (MQ=255) cGGTAACCCGTACAACCTCGGGCAGACCATTACCCAGGGGATTATTAGTGCCACGGGTCGAATCGGTc > 1:2131028/1‑68 (MQ=255) cGGTAACCCGTACAACCTCGGGCAGACCATTACCCAGGGGATTATTAGTGCCACGGGTCGAATCGGTc > 1:185854/1‑68 (MQ=255) cGGTAACCCGTACAACCTCGGGCAGACCATTACCCAGGGGATTATTAGTGCCACGGGTCGAATCGGTc > 1:1186531/1‑68 (MQ=255) cGGTAACCCGTACAACCTCGGGCAGACCATTACCCAGGGGATTATTAGTGCCACGGGTCGAATCGGTc > 1:1215016/1‑68 (MQ=255) cGGTAACCCGTACAACCTCGGGCAGACCATTACCCAGGGGATTATTAGTGCCACGGGTCGAATCGGTc > 1:1220811/1‑68 (MQ=255) cGGTAACCCGTACAACCTCGGGCAGACCATTACCCAGGGGATTATTAGTGCCACGGGTCGAATCGGTc > 1:1046533/1‑68 (MQ=255) cGGTAACCCGTACAACCTCGGGCAGACCATTACCCAGGGGATTATCAGTGCCACGGGTCGAATCGGTCt < 1:1096012/69‑1 (MQ=255) cGGTAACCCGTACAACCTCGGGCAGACCATTACCCAGGGGATTATCAGTGCCACGGGTCGAATCGGTCt < 1:2074125/69‑1 (MQ=255) cGGTAACCCGTACAACCTCGGGCAGACCATTACCCAGGGGATTATCAGTGCCACGGGTCGAATCGGTCt < 1:3275446/69‑1 (MQ=255) cGGTAACCCGTACAACCTCGGGCAGACCATTACCCAGGGGATTATCAGTGCCACGGGTCGAATCGGTCt < 1:2760663/69‑1 (MQ=255) ggTAACCCGTACAACCTCGGGCAGACCATTACCCAGGGGattatt < 1:1592260/45‑1 (MQ=255) tAACCCGTACAACCTCGGGCAGACCATTACCCAGGGGATTATTAGTGCCACGGGTCGAATCGGTCTGa < 1:880829/68‑1 (MQ=255) tAACCCGTACAACCTCGGGCAGACCATTACCCAGGGGATTATTAGTGCCACGGGTCGAATCGGTCTGa < 1:2079132/68‑1 (MQ=255) tAACCCGTACAACCTCGGGCAGACCATTACCCAGGGGATTATTAGTGCCACGGGTCGAATCGGTCTGa < 1:2512971/68‑1 (MQ=255) gCAGACCATTACCCAGGGGATTATTAGTGCCACGGGTCGAATCGGTCTGAACCCGACCGGGCGGCaa < 1:2174969/67‑1 (MQ=255) gCAGACCATTACCCAGGGGATTATTAGTGCCACGGGTCGAATCGGTCTGAACCCGACCGGGCGGCaa < 1:1265875/67‑1 (MQ=255) gCAGACCATTACCCAGGGGATTATTAGTGCCACGGGTCGAATCGGTCTGAACCCGACCGGGCGGCaa < 1:729011/67‑1 (MQ=255) gCAGACCATTACCCAGGGGATTATTAGTGCCACGGGTCGAATCGGTCTGAACCCGACCGGGCGGCaa < 1:1552975/67‑1 (MQ=255) gCAGACCATTACCCAGGGGATTATTAGTGCCACGGGTCGAATCGGTCTGAACCCGACCGGGCGGCaa < 1:2171112/67‑1 (MQ=255) gCAGACCATTACCCAGGGGATTATTAGTGCCACGGGTCGAATCGGTCTGAACCCGACCGGGCGGCaa < 1:659826/67‑1 (MQ=255) gCAGACCATTACCCAGGGGATTATTAGTGCCACGGGTCGAATCGGTCTGAACCCGACCGGGCGGCaa < 1:553477/67‑1 (MQ=255) gCAGACCATTACCCAGGGGATTATTAGTGCCACGGGTCGAATCGGTCTGAACCCGACCGGGCGGCaa < 1:395004/67‑1 (MQ=255) gCAGACCATTACCCAGGGGATTATTAGTGCCACGGGTCGAATCGGTCTGAACCCGACCGGGCGGCaa < 1:1997663/67‑1 (MQ=255) gCAGACCATTACCCAGGGGATTATTAGTGCCACGGGTCGAATCGGTCTGAACCCGACCGGGCGGCaa < 1:1975004/67‑1 (MQ=255) gCAGACCATTACCCAGGGGATTATTAGTGCCACGGGTCGAATCGGTCTGAACCCGACCGGGCGGCaa < 1:385977/67‑1 (MQ=255) cAGACCATTACCCAGGGGATTATTAGTGCCACGGGTCGAATCGGTCTGAACCCGACCGGGCGGCAAAAc < 1:1910023/69‑1 (MQ=255) cAGACCATTACCCAGGGGATTATTAGTGCCACGGGTCGAATCGGTCTGAACCCGACCGGGCGGCAAAAc < 1:1512214/69‑1 (MQ=255) aGACCATTACCCAGGGGATTATTAGTGCCACGGGTCGAATCGGTCTGAACCCGACCGGGCGGCAAAAc < 1:2355372/68‑1 (MQ=255) cATTACCCAGGGGATTATTAGTGCCACGGGTCGAATCGGTCTGAACCCGACCGGGCGGCAAAACTtcc > 1:894686/1‑68 (MQ=255) cATTACCCAGGGGATTATTAGTGCCACGGGTCGAATCGGTCTGAACCCGACCGGGCGGCAAAACTtcc > 1:3002931/1‑68 (MQ=255) cATTACCCAGGGGATTATTAGTGCCACGGGTCGAATCGGTCTGAACCCGACCGGGCGGCAAAACTtcc > 1:2310947/1‑68 (MQ=255) cATTACCCAGGGGATTATTAGTGCCACGGGTCGAATCGGTCTGAACCCGACCGGGCGGCAAAACTtcc > 1:1284323/1‑68 (MQ=255) cATTACCAAGGGGATTATTAGTGCCACGGGTCGAATCGGTCTGAACCCGACCGGGCGGCAAAACTtcc > 1:2737458/1‑68 (MQ=255) ttACCCAGGGGATTATCAGTGCCACTGGTCGAATCGGt > 1:2966829/1‑38 (MQ=38) ttACCCAGGGGATTATCAGTGCCACGGGTCGAATc > 1:178167/1‑35 (MQ=255) ttACCCAGGGGATTATCAGTGCCACGGGTCGAATCgg > 1:2336580/1‑37 (MQ=255) ttACCCAGGGGATTATCAGTGCCACGGGTCGAATCGGt > 1:2700503/1‑38 (MQ=255) ttACCCAGGGGATTATCAGTGCCACGGGTCGAATCGGt > 1:938106/1‑38 (MQ=255) ttACCCAGGGGATTATCAGTGCCACGGGTCGAATCGGt > 1:1072050/1‑38 (MQ=255) ttACCCAGGGGATTATCAGTGCCACGGGTCGAATCGGt > 1:855677/1‑38 (MQ=255) ttACCCAGGGGATTATCAGTGCCACGGGTCGAATCGGt > 1:765869/1‑38 (MQ=255) ttACCCAGGGGATTATCAGTGCCACGGGTCGAATCGGt > 1:1109954/1‑38 (MQ=255) ttACCCAGGGGATTATCAGTGCCACGGGTCGAATCGGt > 1:1310667/1‑38 (MQ=255) ttACCCAGGGGATTATCAGTGCCACGGGTCGAATCGGt > 1:1314609/1‑38 (MQ=255) ttACCCAGGGGATTATCAGTGCCACGGGTCGAATCGGt > 1:1645826/1‑38 (MQ=255) ttACCCAGGGGATTATCAGTGCCACGGGTCGAATCGGt > 1:25187/1‑38 (MQ=255) ttACCCAGGGGATTATCAGTGCCACGGGTCGAATCGGt > 1:2783375/1‑38 (MQ=255) ttACCCAGGGGATTATCAGTGCCACGGGTCGAATCGGt > 1:2926066/1‑38 (MQ=255) ttACCCAGGGGATTATCAGTGCCACGGGTCGAATCGGt > 1:2651389/1‑38 (MQ=255) ttACCCAGGGGATTATCAGTGCCACGGGTCGAATCGGt > 1:263246/1‑38 (MQ=255) ttACCCAGGGGATTATCAGTGCCACGGGTCGAATCGGt > 1:304424/1‑38 (MQ=255) ttACCCAGGGGATTATCAGTGCCACGGGTCGAATCGGt > 1:252372/1‑38 (MQ=255) ttACCCAGGGGATTATCAGTGCCACGGGTCGAATCGGt > 1:3378017/1‑38 (MQ=255) ttACCCAGGGGATTATCAGTGCCACGGGTCGAATCGGt > 1:1032042/1‑38 (MQ=255) ttACCCAGGGGATTATCAGTGCCACGGGTCGAATCGGt > 1:333300/1‑38 (MQ=255) ccAGGGGATTATTAGTGCCACGGGTCGAATCGGTCTGAACCCGACCGGGCGGCAAAACTTCCTCCaaa < 1:1671637/68‑1 (MQ=255) ccAGGGGATTATTAGTGCCACGGGTCGAATCGGTCTGAACCCGACCGGGCGGCAAAACTTCCTCCaaa < 1:2586842/68‑1 (MQ=255) ccAGGGGATTATTAGTGCCACGGGTCGAATCGGTCTGAACCCGACCGGGCGGCAAAACTTCCTCCaaa < 1:403631/68‑1 (MQ=255) ccAGGGGATTATTAGTGCCACGGGTCGAATCGGTCTGAACCCGACCGGGCGGCAAAACTTCCTCCaaa < 1:1103180/68‑1 (MQ=255) ccAGGGGATTATTAGTGCCACGGGTCGAATCGGTCTGAACCCGACCGGGCGGCAAAACTTCCTCCaaa < 1:1889442/68‑1 (MQ=255) ccAGGGGATTATTAGTGCCACGGGTCGAATCGGTCTGAACCCGACCGGGCGGCAAAACTTCCTCCaaa < 1:1098990/68‑1 (MQ=255) ttattaGTGCCACGGGTCGAATCGGTCTGAACCCGACC‑GGCGGCAAAACTTCCTCCAAACCGATGCTTc < 1:2138509/69‑1 (MQ=255) tattaGTGCCACGGGTCGAATCGGTCTGAACCCGACCGGGCGGCAAAACTTCCTCCAAACCGATGCTTc < 1:480920/69‑1 (MQ=255) tattaGTGCCACGGGTCGAATCGGTCTGAACCCGACCGGGCGGCAAAACTTCCTCCAAACCGATGCTTc < 1:2033377/69‑1 (MQ=255) | ATTGGCGACGTAGTACTGGCGATCGGTAACCCGTACAACCTCGGGCAGACCATTACCCAGGGGATTATTAGTGCCACGGGTCGAATCGGTCTGAACCCGACCGGGCGGCAAAACTTCCTCCAAACCGATGCTTC > minE/2197932‑2198065 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |