Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,198,240 C→T 34.8% R253R (CGC→CGT degS → serine endoprotease, periplasmic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,198,2400CT34.8% 63.8 / 47.4 86R253R (CGC→CGTdegSserine endoprotease, periplasmic
Reads supporting (aligned to +/- strand):  ref base C (44/12);  new base T (20/10);  total (64/22)
Fisher's exact test for biased strand distribution p-value = 3.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.38e-01

GTATCGGCTTTGCGATTCCTTTCCAGTTAGCAACCAAAATTATGGATAAGCTGATCCGCGATGGTCGCGTGATCCGCGGCTACATTGGTATCGGCGGACGTGAGATCGCACCACTG  >  minE/2198173‑2198288
                                                                   |                                                
gTATCGGCTTTGCGATTCCTTTCCAGTTAGCAACCAAAATTATGGATAAGCTGATCCGCGATGGTcgc                                                  >  1:2346750/1‑68 (MQ=255)
gTATCGGCTTTGCGATTCCTTTCCAGTTAGCAACCAAAATTATGGATAAGCTGATCCGCGATGGTcgc                                                  >  1:2685100/1‑68 (MQ=255)
gTATCGGCTTTGCGATTCCTTTCCAGTTAGCAACCAAAATTATGGATAAGCTGATCCGCGATGGTcgc                                                  >  1:3165716/1‑68 (MQ=255)
gTATCGGCTTTGCGATTCCTTTCCAGTTAGCAACCAAAATTATGGATAAGCTGATCCGCGATGGTcgc                                                  >  1:3167188/1‑68 (MQ=255)
gTATCGGCTTTGCGATTCCTTTCCAGTTAGCAACCAAAATTATGGATAAGCTGATCCGCGATGGTcgc                                                  >  1:798341/1‑68 (MQ=255)
gTATCGGCTTTGCGATTCCTTTCCAGTTAGCAACCAAAATTATGGATAAGCTGATCCGCGATGGTcgc                                                  >  1:2095039/1‑68 (MQ=255)
gTATCGGCTTTGCGATTCCTTTCCAGTTAGCAACCAAAATTATGGATAAGCTGATCCGCGATGGTcgc                                                  >  1:1348301/1‑68 (MQ=255)
gTATCGGCTTTGCGATTCCTTTCCAGTTAGCAACCAAAATTATGGATAAGCTGATCCGCGATGGTcgc                                                  >  1:393474/1‑68 (MQ=255)
gTATCGGCTTTGCGATTCCTTTCCAGTTAGCAACCAAAATTATGGATAAGCTGATCCGCGATGGTcgc                                                  >  1:2026923/1‑68 (MQ=255)
  aTCGGCTTTGCGATTCCTTTCCAGTTAGCAACCAAAATTATGGATAAGCTGATCCGCGATGGTCGCGt                                                >  1:3426427/1‑68 (MQ=255)
  aTCGGCTTTGCGATTCCTTTCCAGTTAGCAACCAAAATTATGGATAAGCTGATCCGCGATGGTCGCGt                                                >  1:1556228/1‑68 (MQ=255)
  aTCGGCTTTGCGATTCCTTTCCAGTTAGCAACCAAAATTATGGATAAGCTGATCCGCGATGGTCGCGTg                                               >  1:452110/1‑69 (MQ=255)
  aTCGGCTTTGCGATTCCTTTCCAGTTAGCAACCAAAATTATGGATAAGCTGATCCGCGATGGTCGCGTg                                               <  1:3213058/69‑1 (MQ=255)
  aTCGGCTTTGCGATTCCTTTCCAGTTAGCAACCAAAATTATGGATAAGCTGATCCGCGATGGTCGCGTg                                               >  1:2103737/1‑69 (MQ=255)
  aTCGGCTTTGCGATTCCTTTCCAGTTAGCAACCAAAATTATGGATAAGCTGATCCGCGATGGTCGCGTg                                               <  1:2103773/69‑1 (MQ=255)
  aTCGGCTTTGCGATTCCTTTCCAGTTAGCAACCAAAATTATGGATAAGCTGATCCGCGATGGTCGCGTg                                               >  1:2811613/1‑69 (MQ=255)
  aTCGGCTTTGCGATTCCTTTCCAGTTAGCAACCAAAATTATGGATAAGCTGATCCGCGATGGTCGCGTg                                               <  1:240445/69‑1 (MQ=255)
  aTCGGCTTTGCGATTCCTTTCCAGTTAGCAACCAAAATTATGGATAAGCTGATCCGCGATGGTCGCGTg                                               >  1:2797449/1‑69 (MQ=255)
  aTCGGCTTTGCGATTCCTTTCCAGTTAGCAACCAAAATTATGGATAAGCTGATCCGCGATGGTCGCGTg                                               <  1:1930188/69‑1 (MQ=255)
  aTCGGCTTTGCGATTCCTTTCCAGTTAGCAACCAAAATTATGGATAAGCTGATCCGCGATGGTCGCGTg                                               >  1:863455/1‑69 (MQ=255)
  aTCGGCTTTGCGATTCCTTTCCAGTTAGCAACCAAAATTATGGATAAGCTGATCCGCGATGGTCGCGTg                                               <  1:1669862/69‑1 (MQ=255)
  aTCGGCTTTGCGATTCCTTTCCAGTTAGCAACCAAAATTATGGATAAGCTGATCCGCGATGGTCGCGTg                                               >  1:1725709/1‑69 (MQ=255)
  aTCGGCTTTGCGATTCCTTTCCAGTTAGCAACCAAAATTATGGATAAGCTGATCAGCGATGGTCGCGTg                                               >  1:1074454/1‑69 (MQ=255)
              aTTCCTTTCCAGTTAGCAACCAAAATTATGGATAAGCTGATCCGCGATGGTCGCGTGATCCGCGGCTa                                    <  1:26263/68‑1 (MQ=255)
               ttCCTTTCCAGTTAGCAACCAAAATTATGGATAAGCTGATCCGCGATGGTCGCGTGATCCGCGGCTa                                    <  1:77644/67‑1 (MQ=255)
                          ttAGCGACCAAAATTATGGATAAACTGATCCGTGATGGCCGTGTGATCCGTGGCTAcattggcattgg                        >  1:2709895/1‑68 (MQ=255)
                          ttAGCGACCAAAATTATGGATAAACTGATCCGTGATGGCCGTGTGATCCGTGGCTAcattggcattgg                        >  1:2737701/1‑68 (MQ=255)
                          ttAGCGACCAAAATTATGGATAAACTGATCCGTGATGGCCGTGTGATCCGTGGCTAcattggcattgg                        >  1:3061288/1‑68 (MQ=255)
                          ttAGCGACCAAAATTATGGATAAACTGATCCGTGATGGCCGTGTGATCCGTGGCTAcattggcattgg                        >  1:37240/1‑68 (MQ=255)
                          ttAGCGACCAAAATTATGGATAAACTGATCCGTGATGGCCGTGTGATCCGTGGCTAcattggcattgg                        >  1:422234/1‑68 (MQ=255)
                          ttAGCGACCAAAATTATGGATAAACTGATCCGTGATGGCCGTGTGATCCGTGGCTAcattggcattgg                        >  1:2494395/1‑68 (MQ=255)
                          ttAGCGACCAAAATTATGGATAAACTGATCCGTGATGGCCGTGTGATCCGTGGCTAcattggcattgg                        >  1:2425672/1‑68 (MQ=255)
                          ttAGCGACCAAAATTATGGATAAACTGATCCGTGATGGCCGTGTGATCCGTGGCTAcattggcattgg                        >  1:438323/1‑68 (MQ=255)
                          ttAGCGACCAAAATTATGGATAAACTGATCCGTGATGGCCGTGTGATCCGTGGCTAcattggcattgg                        >  1:549232/1‑68 (MQ=255)
                          ttAGCGACCAAAATTATGGATAAACTGATCCGTGATGGCCGTGTGATCCGTGGCTAcattggcattgg                        >  1:752967/1‑68 (MQ=255)
                          ttAGCGACCAAAATTATGGATAAACTGATCCGTGATGGCCGTGTGATCCGTGGCTAcattggcattgg                        >  1:757694/1‑68 (MQ=255)
                          ttAGCGACCAAAATTATGGATAAACTGATCCGTGATGGCCGTGTGATCCGTGGCTAcattggcattgg                        >  1:1012405/1‑68 (MQ=255)
                          ttAGCGACCAAAATTATGGATAAACTGATCCGTGATGGCCGTGTGATCCGTGGCTAcattggcattgg                        >  1:87457/1‑68 (MQ=255)
                          ttAGCGACCAAAATTATGGATAAACTGATCCGTGATGGCCGTGTGATCCGTGGCTAcattggcattgg                        >  1:113637/1‑68 (MQ=255)
                          ttAGCGACCAAAATTATGGATAAACTGATCCGTGATGGCCGTGTGATCCGTGGCTAcattggcattgg                        >  1:11703/1‑68 (MQ=255)
                          ttAGCGACCAAAATTATGGATAAACTGATCCGTGATGGCCGTGTGATCCGTGGCTAcattggcattgg                        >  1:2082366/1‑68 (MQ=255)
                          ttAGCGACCAAAATTATGGATAAACTGATCCGTGATGGCCGTGTGATCCGTGGCTAcattggcattgg                        >  1:1275114/1‑68 (MQ=255)
                          ttAGCGACCAAAATTATGGATAAACTGATCCGTGATGGCCGTGTGATCCGTGGCTAcattggcattgg                        >  1:1348589/1‑68 (MQ=255)
                          ttAGCGACCAAAATTATGGATAAACTGATCCGTGATGGCCGTGTGATCCGTGGCTAcattggcattgg                        >  1:1493182/1‑68 (MQ=255)
                          ttAGCGACCAAAATTATGGATAAACTGATCCGTGATGGCCGTGTGATCCGTGGCTAcattggcattgg                        >  1:1622527/1‑68 (MQ=255)
                           tAGCAACCAAAATTATGGATAAGCTGATCCGCGATGGTCGCGTGATCCGCGGCTACATTGGTATcggcg                      >  1:2184773/1‑69 (MQ=255)
                           tAGCAACCAAAATTATGGATAAGCTGATCCGCGATGGTCGCGTGATCCGCGGCTACATTGGTATcggcg                      >  1:2486039/1‑69 (MQ=255)
                            aGCAACCAAAATTATGGATAAGCTGATCCGCGATGGTCGCGTGATCCGCGGCTACATTGGTATcggcgg                     >  1:2507529/1‑69 (MQ=255)
                            aGCAACCAAAATTATGGATAAGCTGATCCGCGATGGTCGCGTGATCCGCGGCTACATTGGTATcggcgg                     >  1:2671102/1‑69 (MQ=255)
                            aGCAACCAAAATTATGGATAAGCTGATCCGCGATGGTCGCGTGATCCGCGGCTACATTGGTATcggcgg                     >  1:3009184/1‑69 (MQ=255)
                            aGCAACCAAAATTATGGATAAGCTGATCCGCGATGGTCGCGTGATCCGCGGCTACATTGGTATcggcgg                     >  1:1800168/1‑69 (MQ=255)
                            aGCAACCAAAATTATGGATAAGCTGATCCGCGATGGTCGCGTGATCCGCGGCTACATTGGTATcggcgg                     >  1:1645328/1‑69 (MQ=255)
                            aGCAACCAAAATTATGGATAAGCTGATCCGCGATGGTCGCGTGATCCGCGGCTACATTGGTATcggcg                      >  1:2428023/1‑68 (MQ=255)
                               aaCCAAAATTATGGATAAGCTGATCCGCGATGGTCGCGTGATCCGCGGCTACATTGGTATCGGCGGAc                   >  1:3083623/1‑68 (MQ=255)
                                  cAAAATTATGGATAAGCTGATCCGCGATGGTCGCGTGATCCGCGGCTACATTGGTATCGGCGGAc                   >  1:2768212/1‑65 (MQ=255)
                                  cAAAATTATGGATAAGCTGATCCGCGATGGTCGCGTGATCCGCGGCTACATTGGTATCGGCGGAc                   >  1:736118/1‑65 (MQ=255)
                                      aTTATGGATAAGCTGATCCGCGATGGTCGCGTGATCCGCGGCTACATTGGTATcg                         >  1:1329095/1‑55 (MQ=255)
                                      aTTATGGATAAGCTGATCCGCGATGGTCGCGTGATCCGCGGCTACATTGGTATCGGCGGACGTgag              >  1:783800/1‑66 (MQ=255)
                                      aTTATGGATAAGCTGATCCGCGATGGTCGCGTGATCCGCGGCTACATTGGTATCGGCGGACGTgag              >  1:1126991/1‑66 (MQ=255)
                                      aTTATGGATAAGCTGATCCGCGATGGTCGCGTGATCCGCGGCTACATTGGTATCGGCGGACGTgag              >  1:466885/1‑66 (MQ=255)
                                      aTTATGGATAAGCTGATCCGCGATGGTCGCGTGATCCGCGGCTACATTGGTATCGGCGGACGTgag              >  1:204287/1‑66 (MQ=255)
                                      aTTATGGATAAGCTGATCCGCGATGGTCGCGTGATCCGCGGCTACATTGGTATCGGCGGACGTgag              >  1:2551247/1‑66 (MQ=255)
                                      aTTATGGATAAGCTGATCCGCGATGGTCGCGTGATCCGCGGCTACATTGGTATCGGCGGACGTgag              >  1:3237292/1‑66 (MQ=255)
                                      aTTATGGATAAGCTGATCCGCGATGGTCGCGTGATCCGCGGCTACATTGGTATCGGCGGACGTgag              >  1:3231873/1‑66 (MQ=255)
                                      aTTATGGATAAGCTGATCCGCGATGGTCGCGTGATCCGCGGCTACATTGGTATCGGCGGACGTgag              >  1:1432305/1‑66 (MQ=255)
                                      aTTATGGATAAGCTGATCCGCGATGGTCGCGTGATCCGCGGCTACATTGGTATCGGCGGACGTgag              >  1:2285079/1‑66 (MQ=255)
                                      aTTATGGATAAGCTGATCCGCGATGGTCGCGTGATCCGCGGCTACATTGGTATCGGCGGACGTgag              >  1:91353/1‑66 (MQ=255)
                                      aTTATGGATAAGCTGATCCGCGATGGTCGCGTGATCCGCGGCTACATTGGTATCGGCGGACGTgag              >  1:1581708/1‑66 (MQ=255)
                                      aTTATGGATAAGCTGATCCGCGATGGTCGCGTGATCCGCGGCTACATTGGTATCGGCGGACGTga               >  1:1473696/1‑65 (MQ=255)
                                      aTTATGGATAAGCTGATCCGCGATGGTCGCGTGATCCGCGGCTACATTGGTATCGGCGGACGTga               >  1:894326/1‑65 (MQ=255)
                                      aTTATGGATAAGCTGATCCGCGATGGTCGCGTGATCCGCGGCTACATTGGTATCGGCGGACGTga               >  1:103645/1‑65 (MQ=255)
                                      aTTATGGATAAGCTGATACGCGAAGGTCGCGTGATCCGCGGCTACATTgg                              >  1:21370/1‑50 (MQ=255)
                                           ggATAAGCTGATCCGCGATGGTCGCGTGATCCGCGGCTACATTGGTATCGGCGGACGTGAGATCGcac       <  1:1102579/68‑1 (MQ=255)
                                           ggATAAGCTGATCCGCGATGGTCGCGTGATCCGCGGCTACATTGGTATCGGCGGACGTGAGATCGcac       <  1:3127900/68‑1 (MQ=255)
                                           ggATAAGCTGATCCGCGATGGTCGCGTGATCCGCGGCTACATTGGTATCGGCGGACGTGAGATCGcac       <  1:819860/68‑1 (MQ=255)
                                           ggATAAGCTGATCCGCGATGGTCGCGTGATCCGCGGCTACATTGGTATCGGCGGACGTGAGATCGcac       <  1:962678/68‑1 (MQ=255)
                                           ggATAAGCTGATCCGCGATGGTCGCGTGATCCGCGGCTACATTGGTATCGGCGGACGTGAGATCGcac       <  1:2349419/68‑1 (MQ=255)
                                            gATAAGCTGATCCGCGATGGTCGCGTGATCCGCGGCTACATTGGTATCGGCGGACGTGAGATCGcacca     >  1:202966/1‑69 (MQ=255)
                                            gATAAGCTGATCCGCGATGGTCGCGTGATCCGCGGCTACATTGGTATCGGCGGACGTGAGATCGcacca     >  1:3340877/1‑69 (MQ=255)
                                               aaGCTGATCCGCGATGGTCGCGTGATCCGCGGCTACATTGGTATcggc                       >  1:680751/1‑48 (MQ=255)
                                               aaGCTGATCCGCGATGGTCGCGTGATCCGCGGCTACATTGGTATCGGCGGACGTGAGATCGCACCACTg  >  1:3336146/1‑69 (MQ=255)
                                               aaGCTGATCCGCGATGGTCGCGTGATCCGCGGCTACATTGGTATCGGCGGACGTGAGATCGCACCACTg  >  1:2706315/1‑69 (MQ=255)
                                               aaGCTGATCCGCGATGGTCGCGTGATCCGCGGCTACATTGGTATCGGCGGACGTGAGATCGCACCACTg  >  1:1432970/1‑69 (MQ=255)
                                               aaGCTGATCCGCGATGGTCGCGTGATCCGCGGCTACATTGGTATCGGCGGACGTGAGATCGCACCACTg  >  1:1481799/1‑69 (MQ=255)
                                               aaGCTGATCCGCGATGGTCGCGTGATCCGCGGCTACATTGGTATCGGCGGACGTGAGATCGCACCACTg  >  1:2819922/1‑69 (MQ=255)
                                                   tGATCCGTGATGGCCGTGTGATCCGTGGCTACATTGGCATTGGTGGACGTGAGATCg          <  1:1032583/57‑1 (MQ=255)
                                                   tGATCCGTGATGGCCGTGTGATCCGTGGCTACATTGGCATTGGTGGACGTGAGATCg          <  1:641597/57‑1 (MQ=255)
                                                   tGATCCGTGATGGCCGTGTGATCCGTGGCTACATTGGCATTGGTGGACGTGAGATCg          <  1:3213427/57‑1 (MQ=255)
                                                   tGATCCGTGATGGCCGTGTGATCCGTGGCTACATTGGCATTGGTGGACGTGAGATCg          <  1:3045935/57‑1 (MQ=255)
                                                   tGATCCGTGATGGCCGTGTGATCCGTGGCTACATTGGCATTGGTGGACGTGAGATCg          <  1:2715422/57‑1 (MQ=255)
                                                   tGATCCGTGATGGCCGTGTGATCCGTGGCTACATTGGCATTGGTGGACGTGAGATCg          <  1:2325445/57‑1 (MQ=255)
                                                   tGATCCGTGATGGCCGTGTGATCCGTGGCTACATTGGCATTGGTGGACGTGAGATCg          <  1:1934950/57‑1 (MQ=255)
                                                   tGATCCGTGATGGCCGTGTGATCCGTGGCTACATTGGCATTGGTGGACGTGAGATCg          <  1:1532048/57‑1 (MQ=255)
                                                   tGATCCGTGATGGCCGTGTGATCCGTGGCTACATTGGCATTGGTGGACGTGAGATCg          <  1:1318796/57‑1 (MQ=255)
                                                   tGATACGTGATGGCCGTGTGATCCGTGGCTACATTGGCATTGGTGGACGTGAGATCg          <  1:982044/57‑1 (MQ=255)
                                                                   |                                                
GTATCGGCTTTGCGATTCCTTTCCAGTTAGCAACCAAAATTATGGATAAGCTGATCCGCGATGGTCGCGTGATCCGCGGCTACATTGGTATCGGCGGACGTGAGATCGCACCACTG  >  minE/2198173‑2198288

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: