| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,198,249 | C→T | 41.6% | R256R (CGC→CGT) | degS → | serine endoprotease, periplasmic |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,198,249 | 0 | C | T | 41.6% | 27.8 / 49.8 | 72 | R256R (CGC→CGT) | degS | serine endoprotease, periplasmic |
| Reads supporting (aligned to +/- strand): ref base C (35/7); new base T (20/10); total (55/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.58e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.16e-01 | |||||||||||
ATTCCTTTCCAGTTAGCAACCAAAATTATGGATAAGCTGATCCGCGATGGTCGCGTGATCCGCGGCTACATTGGTATCGGCGGACGTGAGATCGCACCACTGCACGCGCAGGGCGGTGGTATAGATCAA > minE/2198187‑2198315 | aTTCCTTTCCAGTTAGCAACCAAAATTATGGATAAGCTGATCCGCGATGGTCGCGTGATCCGCGGCTa < 1:26263/68‑1 (MQ=255) ttCCTTTCCAGTTAGCAACCAAAATTATGGATAAGCTGATCCGCGATGGTCGCGTGATCCGCGGCTa < 1:77644/67‑1 (MQ=255) ttAGCGACCAAAATTATGGATAAACTGATCCGTGATGGCCGTGTGATCCGTGGCTAcattggcattgg > 1:2737701/1‑68 (MQ=255) ttAGCGACCAAAATTATGGATAAACTGATCCGTGATGGCCGTGTGATCCGTGGCTAcattggcattgg > 1:1622527/1‑68 (MQ=255) ttAGCGACCAAAATTATGGATAAACTGATCCGTGATGGCCGTGTGATCCGTGGCTAcattggcattgg > 1:87457/1‑68 (MQ=255) ttAGCGACCAAAATTATGGATAAACTGATCCGTGATGGCCGTGTGATCCGTGGCTAcattggcattgg > 1:2082366/1‑68 (MQ=255) ttAGCGACCAAAATTATGGATAAACTGATCCGTGATGGCCGTGTGATCCGTGGCTAcattggcattgg > 1:2425672/1‑68 (MQ=255) ttAGCGACCAAAATTATGGATAAACTGATCCGTGATGGCCGTGTGATCCGTGGCTAcattggcattgg > 1:2494395/1‑68 (MQ=255) ttAGCGACCAAAATTATGGATAAACTGATCCGTGATGGCCGTGTGATCCGTGGCTAcattggcattgg > 1:2709895/1‑68 (MQ=255) ttAGCGACCAAAATTATGGATAAACTGATCCGTGATGGCCGTGTGATCCGTGGCTAcattggcattgg > 1:1012405/1‑68 (MQ=255) ttAGCGACCAAAATTATGGATAAACTGATCCGTGATGGCCGTGTGATCCGTGGCTAcattggcattgg > 1:757694/1‑68 (MQ=255) ttAGCGACCAAAATTATGGATAAACTGATCCGTGATGGCCGTGTGATCCGTGGCTAcattggcattgg > 1:3061288/1‑68 (MQ=255) ttAGCGACCAAAATTATGGATAAACTGATCCGTGATGGCCGTGTGATCCGTGGCTAcattggcattgg > 1:752967/1‑68 (MQ=255) ttAGCGACCAAAATTATGGATAAACTGATCCGTGATGGCCGTGTGATCCGTGGCTAcattggcattgg > 1:37240/1‑68 (MQ=255) ttAGCGACCAAAATTATGGATAAACTGATCCGTGATGGCCGTGTGATCCGTGGCTAcattggcattgg > 1:422234/1‑68 (MQ=255) ttAGCGACCAAAATTATGGATAAACTGATCCGTGATGGCCGTGTGATCCGTGGCTAcattggcattgg > 1:438323/1‑68 (MQ=255) ttAGCGACCAAAATTATGGATAAACTGATCCGTGATGGCCGTGTGATCCGTGGCTAcattggcattgg > 1:549232/1‑68 (MQ=255) ttAGCGACCAAAATTATGGATAAACTGATCCGTGATGGCCGTGTGATCCGTGGCTAcattggcattgg > 1:1348589/1‑68 (MQ=255) ttAGCGACCAAAATTATGGATAAACTGATCCGTGATGGCCGTGTGATCCGTGGCTAcattggcattgg > 1:1493182/1‑68 (MQ=255) ttAGCGACCAAAATTATGGATAAACTGATCCGTGATGGCCGTGTGATCCGTGGCTAcattggcattgg > 1:1275114/1‑68 (MQ=255) ttAGCGACCAAAATTATGGATAAACTGATCCGTGATGGCCGTGTGATCCGTGGCTAcattggcattgg > 1:11703/1‑68 (MQ=255) ttAGCGACCAAAATTATGGATAAACTGATCCGTGATGGCCGTGTGATCCGTGGCTAcattggcattgg > 1:113637/1‑68 (MQ=255) tAGCAACCAAAATTATGGATAAGCTGATCCGCGATGGTCGCGTGATCCGCGGCTACATTGGTATcggcg > 1:2486039/1‑69 (MQ=255) tAGCAACCAAAATTATGGATAAGCTGATCCGCGATGGTCGCGTGATCCGCGGCTACATTGGTATcggcg > 1:2184773/1‑69 (MQ=255) aGCAACCAAAATTATGGATAAGCTGATCCGCGATGGTCGCGTGATCCGCGGCTACATTGGTATcggcgg > 1:2671102/1‑69 (MQ=255) aGCAACCAAAATTATGGATAAGCTGATCCGCGATGGTCGCGTGATCCGCGGCTACATTGGTATcggcgg > 1:2507529/1‑69 (MQ=255) aGCAACCAAAATTATGGATAAGCTGATCCGCGATGGTCGCGTGATCCGCGGCTACATTGGTATcggcgg > 1:3009184/1‑69 (MQ=255) aGCAACCAAAATTATGGATAAGCTGATCCGCGATGGTCGCGTGATCCGCGGCTACATTGGTATcggcgg > 1:1800168/1‑69 (MQ=255) aGCAACCAAAATTATGGATAAGCTGATCCGCGATGGTCGCGTGATCCGCGGCTACATTGGTATcggcgg > 1:1645328/1‑69 (MQ=255) aGCAACCAAAATTATGGATAAGCTGATCCGCGATGGTCGCGTGATCCGCGGCTACATTGGTATcggcg > 1:2428023/1‑68 (MQ=255) aaCCAAAATTATGGATAAGCTGATCCGCGATGGTCGCGTGATCCGCGGCTACATTGGTATCGGCGGAc > 1:3083623/1‑68 (MQ=255) cAAAATTATGGATAAGCTGATCCGCGATGGTCGCGTGATCCGCGGCTACATTGGTATCGGCGGAc > 1:2768212/1‑65 (MQ=255) cAAAATTATGGATAAGCTGATCCGCGATGGTCGCGTGATCCGCGGCTACATTGGTATCGGCGGAc > 1:736118/1‑65 (MQ=255) aTTATGGATAAGCTGATCCGCGATGGTCGCGTGATCCGCGGCTACATTGGTATcg > 1:1329095/1‑55 (MQ=255) aTTATGGATAAGCTGATCCGCGATGGTCGCGTGATCCGCGGCTACATTGGTATCGGCGGACGTgag > 1:3231873/1‑66 (MQ=255) aTTATGGATAAGCTGATCCGCGATGGTCGCGTGATCCGCGGCTACATTGGTATCGGCGGACGTgag > 1:3237292/1‑66 (MQ=255) aTTATGGATAAGCTGATCCGCGATGGTCGCGTGATCCGCGGCTACATTGGTATCGGCGGACGTgag > 1:1126991/1‑66 (MQ=255) aTTATGGATAAGCTGATCCGCGATGGTCGCGTGATCCGCGGCTACATTGGTATCGGCGGACGTgag > 1:2551247/1‑66 (MQ=255) aTTATGGATAAGCTGATCCGCGATGGTCGCGTGATCCGCGGCTACATTGGTATCGGCGGACGTgag > 1:91353/1‑66 (MQ=255) aTTATGGATAAGCTGATCCGCGATGGTCGCGTGATCCGCGGCTACATTGGTATCGGCGGACGTgag > 1:466885/1‑66 (MQ=255) aTTATGGATAAGCTGATCCGCGATGGTCGCGTGATCCGCGGCTACATTGGTATCGGCGGACGTgag > 1:783800/1‑66 (MQ=255) aTTATGGATAAGCTGATCCGCGATGGTCGCGTGATCCGCGGCTACATTGGTATCGGCGGACGTgag > 1:204287/1‑66 (MQ=255) aTTATGGATAAGCTGATCCGCGATGGTCGCGTGATCCGCGGCTACATTGGTATCGGCGGACGTgag > 1:1581708/1‑66 (MQ=255) aTTATGGATAAGCTGATCCGCGATGGTCGCGTGATCCGCGGCTACATTGGTATCGGCGGACGTgag > 1:1432305/1‑66 (MQ=255) aTTATGGATAAGCTGATCCGCGATGGTCGCGTGATCCGCGGCTACATTGGTATCGGCGGACGTgag > 1:2285079/1‑66 (MQ=255) aTTATGGATAAGCTGATCCGCGATGGTCGCGTGATCCGCGGCTACATTGGTATCGGCGGACGTga > 1:103645/1‑65 (MQ=255) aTTATGGATAAGCTGATCCGCGATGGTCGCGTGATCCGCGGCTACATTGGTATCGGCGGACGTga > 1:894326/1‑65 (MQ=255) aTTATGGATAAGCTGATCCGCGATGGTCGCGTGATCCGCGGCTACATTGGTATCGGCGGACGTga > 1:1473696/1‑65 (MQ=255) aTTATGGATAAGCTGATACGCGAAGGTCGCGTGATCCGCGGCTACATTgg > 1:21370/1‑50 (MQ=255) ggATAAGCTGATCCGCGATGGTCGCGTGATCCGCGGCTACATTGGTATCGGCGGACGTGAGATCGcac < 1:819860/68‑1 (MQ=255) ggATAAGCTGATCCGCGATGGTCGCGTGATCCGCGGCTACATTGGTATCGGCGGACGTGAGATCGcac < 1:3127900/68‑1 (MQ=255) ggATAAGCTGATCCGCGATGGTCGCGTGATCCGCGGCTACATTGGTATCGGCGGACGTGAGATCGcac < 1:2349419/68‑1 (MQ=255) ggATAAGCTGATCCGCGATGGTCGCGTGATCCGCGGCTACATTGGTATCGGCGGACGTGAGATCGcac < 1:1102579/68‑1 (MQ=255) ggATAAGCTGATCCGCGATGGTCGCGTGATCCGCGGCTACATTGGTATCGGCGGACGTGAGATCGcac < 1:962678/68‑1 (MQ=255) gATAAGCTGATCCGCGATGGTCGCGTGATCCGCGGCTACATTGGTATCGGCGGACGTGAGATCGcacca > 1:202966/1‑69 (MQ=255) gATAAGCTGATCCGCGATGGTCGCGTGATCCGCGGCTACATTGGTATCGGCGGACGTGAGATCGcacca > 1:3340877/1‑69 (MQ=255) aaGCTGATCCGCGATGGTCGCGTGATCCGCGGCTACATTGGTATcggc > 1:680751/1‑48 (MQ=255) aaGCTGATCCGCGATGGTCGCGTGATCCGCGGCTACATTGGTATCGGCGGACGTGAGATCGCACCACTg > 1:3336146/1‑69 (MQ=255) aaGCTGATCCGCGATGGTCGCGTGATCCGCGGCTACATTGGTATCGGCGGACGTGAGATCGCACCACTg > 1:2819922/1‑69 (MQ=255) aaGCTGATCCGCGATGGTCGCGTGATCCGCGGCTACATTGGTATCGGCGGACGTGAGATCGCACCACTg > 1:2706315/1‑69 (MQ=255) aaGCTGATCCGCGATGGTCGCGTGATCCGCGGCTACATTGGTATCGGCGGACGTGAGATCGCACCACTg > 1:1432970/1‑69 (MQ=255) aaGCTGATCCGCGATGGTCGCGTGATCCGCGGCTACATTGGTATCGGCGGACGTGAGATCGCACCACTg > 1:1481799/1‑69 (MQ=255) tGATCCGTGATGGCCGTGTGATCCGTGGCTACATTGGCATTGGTGGACGTGAGATCg < 1:1032583/57‑1 (MQ=255) tGATCCGTGATGGCCGTGTGATCCGTGGCTACATTGGCATTGGTGGACGTGAGATCg < 1:641597/57‑1 (MQ=255) tGATCCGTGATGGCCGTGTGATCCGTGGCTACATTGGCATTGGTGGACGTGAGATCg < 1:3213427/57‑1 (MQ=255) tGATCCGTGATGGCCGTGTGATCCGTGGCTACATTGGCATTGGTGGACGTGAGATCg < 1:3045935/57‑1 (MQ=255) tGATCCGTGATGGCCGTGTGATCCGTGGCTACATTGGCATTGGTGGACGTGAGATCg < 1:2715422/57‑1 (MQ=255) tGATCCGTGATGGCCGTGTGATCCGTGGCTACATTGGCATTGGTGGACGTGAGATCg < 1:2325445/57‑1 (MQ=255) tGATCCGTGATGGCCGTGTGATCCGTGGCTACATTGGCATTGGTGGACGTGAGATCg < 1:1934950/57‑1 (MQ=255) tGATCCGTGATGGCCGTGTGATCCGTGGCTACATTGGCATTGGTGGACGTGAGATCg < 1:1532048/57‑1 (MQ=255) tGATCCGTGATGGCCGTGTGATCCGTGGCTACATTGGCATTGGTGGACGTGAGATCg < 1:1318796/57‑1 (MQ=255) tGATACGTGATGGCCGTGTGATCCGTGGCTACATTGGCATTGGTGGACGTGAGATCg < 1:982044/57‑1 (MQ=255) cgcgGCTACATTGGTATCGGCGGACGTGAGATCGCACCACTGCACGCGCAGGGCGGTGGTATAGATCaa < 1:3426088/69‑1 (MQ=255) cgcgGCTACATTGGTATCGGCGGACGTGAGATCGCACCACTGCACGCGCAGGGCGGTGGTATAGATCaa < 1:758788/69‑1 (MQ=255) cgcgGCTACATTGGTATCGGCGGACGTGAGATCGCACCACTGCACGCGCAGGGCGGTGGTATAGATCaa < 1:2489312/69‑1 (MQ=255) cgcgGCTACATTGGTATCGGCGGACGTGAGATCGCACCACTGCACGCGCAGGGCGGTGGTATAGATCaa < 1:1682039/69‑1 (MQ=255) | ATTCCTTTCCAGTTAGCAACCAAAATTATGGATAAGCTGATCCGCGATGGTCGCGTGATCCGCGGCTACATTGGTATCGGCGGACGTGAGATCGCACCACTGCACGCGCAGGGCGGTGGTATAGATCAA > minE/2198187‑2198315 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |