| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,200,383 | C→T | 36.5% | T133T (ACC→ACT) | argR → | DNA‑binding transcriptional dual regulator, L‑arginine‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,200,383 | 0 | C | T | 36.5% | 52.2 / 49.1 | 82 | T133T (ACC→ACT) | argR | DNA‑binding transcriptional dual regulator, L‑arginine‑binding |
| Reads supporting (aligned to +/- strand): ref base C (33/19); new base T (24/6); total (57/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.41e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.02e-01 | |||||||||||
ACTGGGCAAAGCAGAAGGTATTCTGGGCACCATCGCTGGCGATGACACCATCTTTACCACCCCTGCTAACGGTTTCACAGTCAAAGACCTGTACGAAGCGATTTTAGAGCTGTTCGACCAGGAG > minE/2200326‑2200449 | aCTGGGCAAAGCAGAAGGTATTCTGGGCACCATCGCTGGCGATGACACCATCTTTACCACCCCTGCTaa > 1:1355708/1‑69 (MQ=255) ggTATTCTGGGCACCATCGCTGGCGATGACACCATCTTTACCACCCCTGCTAACGGTTTCACAGTCaaa > 1:369296/1‑69 (MQ=255) ggTATTCTGGGCACCATCGCTGGCGATGACACCATCTTTACCACCCCTGCTAACGGTTTCACAGTCaaa > 1:608338/1‑69 (MQ=255) ggTATTCTGGGCACCATCGCTGGCGATGACACCATCTTTACCACCCCTGCTAACGGTTTCACAGTCaaa > 1:1342093/1‑69 (MQ=255) ggTATTCTGGGCACCATCGCTGGCGATGACACCATCTTTACCACCCCTGCTAACGGTTTCACAGTCaaa > 1:3005433/1‑69 (MQ=255) ggTATTCTGGGCACCATCGCTGGCGATGACACCATCTTTACCACCCCTGCTAACGGTTTCACAGTCaaa > 1:874862/1‑69 (MQ=255) tATTCTGGGCACCATCGCTGGCGATGACACCATCTTTACCACCCCTGCTAACGGTTTCACAGTCAAAGa > 1:225381/1‑69 (MQ=255) tATTCTGGGCACCATCGCTGGCGATGACACCATCTTTACCACCCCTGCTAACGGTTTCACAGTCAAAGa > 1:1931256/1‑69 (MQ=255) tATTCTGGGCACCATCGCTGGCGATGACACCATCTTTACCACCCCTGCTAACGGTTTCACAGTCAAAGa > 1:740384/1‑69 (MQ=255) tATTCTGGGCACCATCGCTGGCGATGACACCATCTTTACCACCCCTGCTAACGGTTTCACAGTCAAAGa > 1:3080301/1‑69 (MQ=255) tATTCTGGGCACCATCGCTGGCGATGACACCATCTTTACCACCCCTGCTAACGGTTTCACAGTCAAAGa > 1:1534122/1‑69 (MQ=255) tATTCTGGGCACCATAGCTGGCGATGACACCATCTTTACCACCCCTGCTAACGGTTTCACAGTCAAAGa > 1:3144924/1‑69 (MQ=255) aTTCTGGGCACCATCGCTGGCGATGACACCATCTTTACCACCCCTGCTAACGGTTTCACAGTCAAAGAc < 1:2142825/69‑1 (MQ=255) aTTCTGGGCACCATCGCTGGCGATGACACCATCTTTACCACCCCTGCTAACGGTTTCACAGTCAAAGAc < 1:2618271/69‑1 (MQ=255) aTTCTGGGCACCATCGCTGGCGATGACACCATCTTTACCACCCCTGCTAACGGTTTCACAGTCAAAGAc < 1:1739609/69‑1 (MQ=255) aTTCTGGGCACCATCGCTGGCGATGACACCATCTTTACCACCCCTGCTAACGGTTTCACAGTCAAAGAc < 1:1966639/69‑1 (MQ=255) aTTCTGGGCACCATCGCTGGCGATGACACCATCTTTACCACCCCTGCTAACGGTTTCACAGTCAAAGAc < 1:745309/69‑1 (MQ=255) aTTCTGGGCACCATCGCTGGCGATGACACCATCTTTACCACCCCTGCTAACGGTTTCACAGTCAAAGAc < 1:2192949/69‑1 (MQ=255) aTTCTGGGCACCATCGCTGGCGATGACACCATCTTTACCACCCCTGCTAACGGTTTCACAGTCAAAGAc < 1:1858191/69‑1 (MQ=255) aTTCTGGGCACCATCGCTGGCGATGACACCATCTTTACCACCCCTGCTAACGGTTTCACAGTCAAAGAc < 1:1881975/69‑1 (MQ=255) aTCGCTGGCGATGACACCATCTTTACCACCCCTGCTAACGGTTTCACAGTCAAAGACCTGTACGAAGCg > 1:422892/1‑69 (MQ=255) aTCGCTGGCGATGACACCATCTTTACCACCCCTGCTAACGGTTTCACAGTCAAAGACCTGTACGAAGCg > 1:2173666/1‑69 (MQ=255) aTCGCTGGCGATGACACCATCTTTACCACCCCTGCTAACGGTTTCACAGTCAAAGACCTGTACGAAGCg > 1:977436/1‑69 (MQ=255) aTCGCTGGCGATGACACCATCTTTACCACCCCTGCTAACGGTTTCACAGTCAAAGACCTGTACGAAGCg > 1:3017182/1‑69 (MQ=255) aTCGCTGGCGATGACACCATCTTTACCACCCCTGCTAACGGTTTCACAGTCAAAGACCTGTACGAAGCg > 1:3063008/1‑69 (MQ=255) aTCGCTGGCGATGACACCATCTTTACCACCCCTGCTAACGGTTTCACAGTCAAAGACCTGTACGAAGCg > 1:835810/1‑69 (MQ=255) aTCGCTGGCGATGACACCATCTTTACCACCCCTGCTAACGGTTTCACAGTCAAAGACCTGTACGAAGCg > 1:1450439/1‑69 (MQ=255) aTCGCTGGCGATGACACCATCTTTACCACCCCTGCTAACGGTTTCACAGTCAAAGACCTGTACGAAGCg > 1:3245695/1‑69 (MQ=255) aTCGCTGGCGATGACACCATCTTTACCACCCCTGCTAACGGTTTCACAGTCAAAGACCTGTACGAAGCg > 1:3281508/1‑69 (MQ=255) aTCGCTGGCGATGACACCATCTTTACCACCCCTGCTAACGGTTTCACAGTCAAAGACCTGTACGAAGCg > 1:1092159/1‑69 (MQ=255) tGGCGATGACACCATCTTTACCACCCCTGCTAACGGTTTCACAGTCAAAGACCTGTACGAAGCGAttt < 1:816822/68‑1 (MQ=255) tGGCGATGACACCATCTTTACCACCCCTGCTAACGGTTTCACAGTCAAAGACCTGTACGAAGCGAttt < 1:833367/68‑1 (MQ=255) tGGCGATGACACCATCTTTACCACCCCTGCTAACGGTTTCACAGTCAAAGACCTGTACGAAGCGAttt < 1:2644965/68‑1 (MQ=255) tGGCGATGACACCATCTTTACCACCCCTGCTAACGGTTTCACAGTCAAAGACCTGTACGAAGCGAttt < 1:1354021/68‑1 (MQ=255) tGGCGATGACACCATCTTTACCACCCCTGCTAACGGTTTCACAGTCAAAGACCTGTACGAAGCGAttt < 1:976177/68‑1 (MQ=255) tGGCGATGACACCATCTTTACCACCCCTGCTAACGGTTTCACAGTCAAAGACCTGTACGAAGCGAttt < 1:2349836/68‑1 (MQ=255) ggCGATGACACCATCTTTACTACCCCTGCTAACGGTTTCACCGTCAAAGAGCTGTACGAAGCGATTTTa < 1:463315/69‑1 (MQ=255) ggCGATGACACCATCTTTACTACCCCTGCTAACGGTTTCACCGTCAAAGAGCTGTACGAAGCGATTTTa < 1:2142121/69‑1 (MQ=255) ggCGATGACACCATCTTTACTACCCCTGCTAACGGTTTCACCGTCAAAGAGCTGTACGAAGCGATTTTa < 1:543207/69‑1 (MQ=255) ggCGATGACACCATCTTTACTACCCCTGCTAACGGTTTCACCGTCAAAGAGCTGTACGAAGCGATTTTa < 1:2060123/69‑1 (MQ=255) gCGATGACACCATCTTTACTACCCCTGCTAACGGTTTCACCGTCAAAGAGCTGTACGAAGCGATTTTa < 1:3347565/68‑1 (MQ=255) gCGATGACACCATCTTTACTACCCCTGCTAACGGTTTCACCGTCAAAGAGCTGTACGAAGCGATTTTa < 1:1775896/68‑1 (MQ=255) gATGACACCATCTTTACCACCCCTGCTAACGGTTTCACAGTCAAAGACCTGTACGAAGCGATTTTagag > 1:58832/1‑69 (MQ=255) gATGACACCATCTTTACCACCCCTGCTAACGGTTTCACAGTCAAAGACCTGTACGAAGCGATTTTagag > 1:141223/1‑69 (MQ=255) gATGACACCATCTTTACCACCCCTGCTAACGGTTTCACAGTCAAAGACCTGTACGAAGCGATTTTagag > 1:3416323/1‑69 (MQ=255) gATGACACCATCTTTACCACCCCTGCTAACGGTTTCACAGTCAAAGACCTGTACGAAGCGATTTTagag > 1:167177/1‑69 (MQ=255) gATGACACCATCTTTACCACCCCTGCTAACGGTTTCACAGTCAAAGACCTGTACGAAGCGATTTTagag > 1:540286/1‑69 (MQ=255) gATGACACCATCTTTACCACCCCTGCTAACGGTTTCACAGTCAAAGACCTGTACGAAGCGATTTTagag > 1:2622914/1‑69 (MQ=255) gATGACACCATCTTTACCACCCCTGCTAACGGTTTCACAGTCAAAGACCTGTACGAAGCGATTTTaga > 1:2358653/1‑68 (MQ=255) tGACACCATCTTTACTACCCCTGCTAACTGTTTCACCGTCAAAGAGCTg > 1:195965/1‑49 (MQ=37) tGACACCATCTTTACTACCCCTGCTAACGGTTTCACCGTCAAAGAGCt > 1:3063877/1‑48 (MQ=37) tGACACCATCTTTACTACCCCTGCTAACGGTTTCACCGTCAAAGAGCTg > 1:683295/1‑49 (MQ=37) tGACACCATCTTTACTACCCCTGCTAACGGTTTCACCGTCAAAGAGCTg > 1:686820/1‑49 (MQ=37) tGACACCATCTTTACTACCCCTGCTAACGGTTTCACCGTCAAAGAGCTg > 1:741932/1‑49 (MQ=37) tGACACCATCTTTACTACCCCTGCTAACGGTTTCACCGTCAAAGAGCTg > 1:1054745/1‑49 (MQ=37) tGACACCATCTTTACTACCCCTGCTAACGGTTTCACCGTCAAAGAGCTg > 1:541548/1‑49 (MQ=37) tGACACCATCTTTACTACCCCTGCTAACGGTTTCACCGTCAAAGAGCTg > 1:870366/1‑49 (MQ=37) tGACACCATCTTTACTACCCCTGCTAACGGTTTCACCGTCAAAGAGCTg > 1:3411267/1‑49 (MQ=37) tGACACCATCTTTACTACCCCTGCTAACGGTTTCACCGTCAAAGAGCTg > 1:664050/1‑49 (MQ=37) tGACACCATCTTTACTACCCCTGCTAACGGTTTCACCGTCAAAGAGCTg > 1:962104/1‑49 (MQ=37) tGACACCATCTTTACTACCCCTGCTAACGGTTTCACCGTCAAAGAGCTg > 1:1642961/1‑49 (MQ=37) tGACACCATCTTTACTACCCCTGCTAACGGTTTCACCGTCAAAGAGCTg > 1:2056329/1‑49 (MQ=37) tGACACCATCTTTACTACCCCTGCTAACGGTTTCACCGTCAAAGAGCTg > 1:2107426/1‑49 (MQ=37) tGACACCATCTTTACTACCCCTGCTAACGGTTTCACCGTCAAAGAGCTg > 1:3342834/1‑49 (MQ=37) tGACACCATCTTTACTACCCCTGCTAACGGTTTCACCGTCAAAGAGCTg > 1:216567/1‑49 (MQ=37) tGACACCATCTTTACTACCCCTGCTAACGGTTTCACCGTCAAAGAGCTg > 1:1159966/1‑49 (MQ=37) tGACACCATCTTTACTACCCCTGCTAACGGTTTCACCGTCAAAGAGCTg > 1:1751675/1‑49 (MQ=37) tGACACCATCTTTACTACCCCTGCTAACGGTTTCACCGTCAAAGAGCTg > 1:2722062/1‑49 (MQ=37) tGACACCATCTTTACTACCCCTGCTAACGGTTTCACCGTCAAAGAGCTg > 1:2897518/1‑49 (MQ=37) tGACACCATCTTTACTACCCCTGCTAACGGTTTCACCGTCAAAGAGCTg > 1:1682244/1‑49 (MQ=37) tGACACCATCTTTACTACCCCTGCTAACGGTTTCACCGTCAAAGAGCTg > 1:2978166/1‑49 (MQ=37) tGACACCATCTTTACTACCCCTGCTAACGGTTTCACCGTCAAAGAGCTg > 1:3007790/1‑49 (MQ=37) tGACACCATCTTTACTACCCCTGCTAACGGTTTCACCGTCAAAGAGCTg > 1:1026490/1‑49 (MQ=37) gACACCATCTTTACCACCCCTGCTAACGGTTTCACAGTCAAAGACCTGTACGAAGCGATTTTAGAGCTg < 1:9243/69‑1 (MQ=255) gACACCATCTTTACCACCCCTGCTAACGGTTTCACAGTCAAAGACCTGTACGAAGCGATTTTAGAGCTg < 1:1105570/69‑1 (MQ=255) gACACCATCTTTACCACCCCTGCTAACGGTTTCACAGTCAAAGACCTGTACGAAGCGATTTTAGAGCTg < 1:1753466/69‑1 (MQ=255) acacCATCTTTACCACCCCTGCTAACGGTTTCACAGTCAAAGACCTGTACGAAGCGATTTTAGAGCTg < 1:217935/68‑1 (MQ=255) acCATCTTTACCACCCCTGCTAACGGTTTCACAGTCAAAGACCTGTACGAAGCGATTTTAGAGCTGTTc < 1:178404/69‑1 (MQ=255) tttACCACCCCTGCTAACGGTTTCACAGTCAAAGCCATGTACGAAGCGATTTTAGAGCTGTTCGAcc > 1:997627/1‑67 (MQ=255) tttACCACCCCTGCTAACGGTTTCACAGTCAAAGACCTGTACGAAGCGATTTTAGAGCTg > 1:2633055/1‑60 (MQ=255) tttACCACCCCTGCTAACGGTTTCACAGTCAAAGACCTGTACGAAGCGATTTTAGAGCTGTTCGAcc > 1:770009/1‑67 (MQ=255) tttACCACCCCTGCTAACGGTTTCACAGTCAAAGACCTGTACGAAGCGATTTTAGAGCTGTTCGAcc > 1:1578213/1‑67 (MQ=255) ccaccCCTGCTAACGGTTTCACAGTCAAAGACCTGTACGAAGCGATTTTAGAGCTGTTCGAGCAGGAg < 1:435904/68‑1 (MQ=255) ccaccCCTGCTAACGGTTTCACAGTCAAAGACCTGTACGAAGCGATTTTAGAGCTGTTCGACCAGGAg < 1:1574575/68‑1 (MQ=255) ccaccCCTGCTAACGGTTTCACAGTCAAAGACCTGTACGAAGCGATTTTAGAGCTGTTCGACCAGGAg < 1:2132053/68‑1 (MQ=255) ccaccCCTGCTAACGGTTTCACAGTCAAAGACCTGTACGAAGCGATTTTAGAGCTGTTCGACCAGGAg < 1:227021/68‑1 (MQ=255) ccaccCCTGCTAACGGTTTCACAGTCAAAGACCTGTACGAAGCGATTTTAGAGCTGTTCGACCAGGAg < 1:2443505/68‑1 (MQ=255) ccaccCCTGCTAACGGTTTCACAGTCAAAGACCTGTACGAAGCGATTTTAGAGCTGTTCGACCAGGAg < 1:38618/68‑1 (MQ=255) ccaccCCTGCTAACGGTTTCACAGTCAAAGACCTGTACGAAGCGATTTTAGAGCTGTTCGACCAGGAg < 1:3132679/68‑1 (MQ=255) ccaccCCTGCTAACGGTTTCACAGTCAAAGACCTGTACGAAGCGATTTTAGAGCTGTTCGACCAGGAg < 1:3197044/68‑1 (MQ=255) caccCCTGCTAACGGTTTCACAGTCAAAGACCTGTACGAAGCGATTTTAGAGCTGTTCGACCAGGAg < 1:670487/67‑1 (MQ=255) | ACTGGGCAAAGCAGAAGGTATTCTGGGCACCATCGCTGGCGATGACACCATCTTTACCACCCCTGCTAACGGTTTCACAGTCAAAGACCTGTACGAAGCGATTTTAGAGCTGTTCGACCAGGAG > minE/2200326‑2200449 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |