| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,202,379 | G→A | 25.2% | S364S (TCC→TCT) | aaeB ← | p‑hydroxybenzoic acid efflux system component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,202,379 | 0 | G | A | 25.2% | 129.8 / 39.8 | 107 | S364S (TCC→TCT) | aaeB | p‑hydroxybenzoic acid efflux system component |
| Reads supporting (aligned to +/- strand): ref base G (19/60); new base A (8/19); total (27/80) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.13e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 109 total aligned reads displayed.
CATCACCATTGCACCACTGCCGGAAGTCCAGCCCGTCCACAGCCAGAAAAGCGTGCCCAGAATGCAGGAAAGTGTGGTTCGCCAGAAGTTAACCATTGCATGATGACGTTCGGCTGACTCTACTTTTACTTCCGG > minE/2202313‑2202447 | catcaCCATTGCACCACTGCCGGAAGTCCAGCCCGTCCACAGCCAGAAAAGCGTGCCCAGAATGCAg > 1:2905027/1‑67 (MQ=255) caCCATTGCACCACTGCCGGAAGTCCAGCCCGTCCACAGCCAGAAAAGCGTGCCCAGAATGCAGGAAAg > 1:1032386/1‑69 (MQ=255) gCACCACTGCCGGAAGTCTAGCCCGTCCACAGCCAGAAAAGCGTGCCCAGAATGCAGGAAAGTGTGGtt > 1:2903850/1‑69 (MQ=255) gCACCACTGCCGGAAGTCCAGCCCGTCCACAGCCAGAAAAGCGTGTCCAGAATGCAGGAAAGTGTGGt > 1:2299214/1‑68 (MQ=255) gCACCACTGCCGGAAGTCCAGCCCGTCCACAGCCAGAAAAGCGTGCCCAGAATGCAGGAAAGTGTGGtt > 1:1110975/1‑69 (MQ=255) gCACCACTGCCGGAAGTCCAGCCCGTCCACAGCCAGAAAAGCGTGCCCAGAATGCAGGAAAGTGTGGtt > 1:2409780/1‑69 (MQ=255) gCACCACTGCCGGAAGTCCAGCCCGTCCACAGCCAGAAAAGCGTGCCCAGAATGCAGGAAAGTGTGGtt > 1:144520/1‑69 (MQ=255) gCACCACTGCCGGAAGTCCAGCCCGTCCACAGCCAGAAAAGCGTGCCCAGAATGCAGGAAAGTGTGGtt > 1:1186933/1‑69 (MQ=255) gCACCACTGCCGGAAGTCCAGCCCGTCCACAGCCAGAAAAGCGTGCCCAGAATGCAGGAAAGTGTGGtt > 1:1404475/1‑69 (MQ=255) gCACCACTGCCGGAAGTCCAGCCCGTCCACAGCCAGAAAAGCGTGCCCAGAATGCAGGAAAGTGTGGtt > 1:3012263/1‑69 (MQ=255) gCACCACTGCCGGAAGTCCAGCCCGTCCACAGCCAGAAAAGCGTGCCCAGAATGCAGGAAAGTGTGGt > 1:2897714/1‑68 (MQ=255) aaGTCCAGCCCGTCCACAGCCAGAAAAGCGTGCCCAGAATGCAGGAAAGTGTGGTTCGCCAGAAGTTaa < 1:2826063/69‑1 (MQ=255) aaGTCCAGCCCGTCCACAGCCAGAAAAGCGTGCCCAGAATGCAGGAAAGTGTGGTTCGCCAGAAGTTaa < 1:2693737/69‑1 (MQ=255) aaGTCCAGCCCGTCCACAGCCAGAAAAGCGTGCCCAGAATGCAGGAAAGTGTGGTTCGCCAGAAGTTaa < 1:2596877/69‑1 (MQ=255) aaGTCCAGCACGTCCACAGCCAGAAAAGCGTGCCCAGAATGCAGGAAAGTGTGGTTCGCCAGAAGTTaa < 1:195513/69‑1 (MQ=255) aGTCCAGCCCGTCCACAGCCAGAAAAGCGTGCCCAGAATGCATGAAAGTGTGGTTCGCCAGAAGTTAAc < 1:135139/69‑1 (MQ=255) aGTCCAGCCCGTCCACAGCCAGAAAAGCGTGCCCAGAATGCAGGGAAGTGTGGTTCGCCAGAAGTTAAc < 1:3085559/69‑1 (MQ=255) aGTCCAGCCCGTCCACAGCCAGAAAAGCGTGCCCAGAATGCAGGAAAGTGTGGTTCGCCAGAAGTTAAc < 1:2168649/69‑1 (MQ=255) aGTCCAGCCCGTCCACAGCCAGAAAAGCGTGCCCAGAATGCAGGAAAGTGTGGTTCGCCAGAAGTTAAc < 1:3040714/69‑1 (MQ=255) aGTCCAGCCCGTCCACAGCCAGAAAAGCGTGCCCAGAATGCAGGAAAGTGTGGTTCGCCAGAAGTTAAc < 1:2207994/69‑1 (MQ=255) aGTCCAGCCCGTCCACAGCCAGAAAAGCGTGCCCAGAATGCAGGAAAGTGTGGTTCGCCAGAAGTTAAc < 1:2246684/69‑1 (MQ=255) aGTCCAGCCCGTCCACAGCCAGAAAAGCGTGCCCAGAATGCAGGAAAGTGTGGTTCGCCAGAAGTTAAc < 1:457486/69‑1 (MQ=255) aGTCCAGCCCGTCCACAGCCAGAAAAGCGTGCCCAGAATGCAGGAAAGTGTGGTTCGCCAGAAGTTAAc < 1:2306711/69‑1 (MQ=255) aGTCCAGCCCGTCCACAGCCAGAAAAGCGTGCCCAGAATGCAGGAAAGTGTGGTTCGCCAGAAGTTAAc < 1:2326769/69‑1 (MQ=255) aGTCCAGCCCGTCCACAGCCAGAAAAGCGTGCCCAGAATGCAGGAAAGTGTGGTTCGCCAGAAGTTAAc < 1:2340027/69‑1 (MQ=255) aGTCCAGCCCGTCCACAGCCAGAAAAGCGTGCCCAGAATGCAGGAAAGTGTGGTTCGCCAGAAGTTAAc < 1:377460/69‑1 (MQ=255) aGTCCAGCCCGTCCACAGCCAGAAAAGCGTGCCCAGAATGCAGGAAAGTGTGGTTCGCCAGAAGTTAAc < 1:2447048/69‑1 (MQ=255) aGTCCAGCCCGTCCACAGCCAGAAAAGCGTGCCCAGAATGCAGGAAAGTGTGGTTCGCCAGAAGTTAAc < 1:2642226/69‑1 (MQ=255) aGTCCAGCCCGTCCACAGCCAGAAAAGCGTGCCCAGAATGCAGGAAAGTGTGGTTCGCCAGAAGTTAAc < 1:2844016/69‑1 (MQ=255) aGTCCAGCCCGTCCACAGCCAGAAAAGCGTGCCCAGAATGCAGGAAAGTGTGGTTCGCCAGAAGTTAAc < 1:64693/69‑1 (MQ=255) aGTCCAGCCCGTCCACAGCCAGAAAAGCGTGCCCAGAATGCAGGAAAGTGTGGTTCGCCAGAAGTTAAc < 1:846306/69‑1 (MQ=255) aGTCCAGCCCGTCCACAGCCAGAAAAGCGTGCCCAGAATGCAGGAAAGTGTGGTTCGCCAGAAGTTAAc < 1:3155050/69‑1 (MQ=255) aGTCCAGCCCGTCCACAGCCAGAAAAGCGTGCCCAGAATGCAGGAAAGTGTGGTTCGCCAGAAGTTAAc < 1:774738/69‑1 (MQ=255) aGTCCAGCCCGTCCACAGCCAGAAAAGCGTGCCCAGAATGCAGGAAAGTGTGGTTCGCCAGAAGTTAAc < 1:3124856/69‑1 (MQ=255) aGTCCAGCCCGTCCACAGCCAGAAAAGCGTGCCCAGAATGCAGGAAAGTGTGGTTCGCCAGAAGTTAAc < 1:1842373/69‑1 (MQ=255) aGTCCAGCCCGTCCACAGCCAGAAAAGCGTGCCCAGAATGCAGGAAAGTGTGGTTCGCCAGAAGTTAAc < 1:1590848/69‑1 (MQ=255) aGTCCAGCCCGTCCACAGCCAGAAAAGCGTGCCCAGAATGCAGGAAAGTGTGGTTCGCCAGAAGTTAAc < 1:107554/69‑1 (MQ=255) aGTCCAGCCCGTCCACAGCCAGAAAAGCGTGCCCAGAATGCAGGAAAGTGTGGTTCGCCAGAAGTTAAc < 1:1040362/69‑1 (MQ=255) aGTCCAGCCCGTCCACAGCCAGAAAAGCGTGCCCAGAATGCAGGAAAGTGTGGTTCGCCAGAAGTTAAc < 1:1898516/69‑1 (MQ=255) aGTCCAGCCCGTCCACAGCCAGAAAAGCGTGCCCAGAATGCAGGAAAGTGTGGTTCGCCAGAAGTTAAc < 1:2015334/69‑1 (MQ=255) aGTCCAGCCCGTCCACAGCCAGAAAAGCGTGCCCAGAATGCAGGAAAGTGTGGTTCGCCAGAAGTTAAc < 1:1465927/69‑1 (MQ=255) ccGTCCACAGCCAGAAAAGCTTGCCCAGAATGCAAGAAAGTGTGGTTCGCCAGAAGTTAACCATTGCat > 1:3311052/1‑69 (MQ=255) ccGTCCACAGCCAGAAAAGCGTGCCCAGAATGCAGGAAAGTGTGGTTCGCCAGAAGTTAAc < 1:3040716/61‑1 (MQ=255) ccGTCCACAGCCAGAAAAGCGTGCCCAGAATGCAAGAAAGTGTGGTTCGCCAGAAGTTAACCATTGCat > 1:3275291/1‑69 (MQ=255) ccGTCCACAGCCAGAAAAGCGTGCCCAGAATGCAAGAAAGTGTGGTTCGCCAGAAGTTAACCATTGCat > 1:2405267/1‑69 (MQ=255) ccGTCCACAGCCAGAAAAGCGTGCCCAGAATGCAAGAAAGTGTGGTTCGCCAGAAGTTAACCATTGCat > 1:46367/1‑69 (MQ=255) ccGTCCACAGCCAGAAAAGCGTGCCCAGAATGCAAGAAAGTGTGGTTCGCCAGAAGTTAACCATTGCat > 1:2803306/1‑69 (MQ=255) ccGTCCACAGCCAGAAAAGCGTGCCCAGAATGCAAGAAAGTGTGGTTCGCCAGAAGTTAACCATTGCat > 1:2780333/1‑69 (MQ=255) ccGTCCACAGCCAGAAAAGCGTGCCCAGAATGCAAGAAAGTGTGGTTCGCCAGAAGTTAACCATTGCat > 1:45541/1‑69 (MQ=255) ccGTCCACAGCCAGAAAAGCGTGCCCAGAATGCAAGAAAGTGTGGTTCGCCAGAAGTTAACCATTGCat > 1:1430556/1‑69 (MQ=255) gTCCACAGCCAGAAAAGCGTG‑CCAGAATGCAGGAAAGTGTGGTTCGCCAGAAGTTAACCATTGCatgat < 1:3070349/69‑1 (MQ=255) tctacaGCCAGAAAAGCGTGCCCAGAATGCAGGAAAGTGTGGTTCGCCAGAAGTTAACCATTGCatgat < 1:3371044/66‑1 (MQ=255) tCCACAGCCAGAAAAGCGTGCCCAGAATGCAGGAAAGTGTGGTTCGCCAGAAGTTAACCATTGCatgat < 1:308066/69‑1 (MQ=255) tCCACAGCCAGAAAAGCGTGCCCAGAATGCAGGAAAGTGTGGTTCGCCAGAAGTTAACCATTGCatgat < 1:3137901/69‑1 (MQ=255) tCCACAGCCAGAAAAGCGTGCCCAGAATGCAGGAAAGTGTGGTTCGCCAGAAGTTAACCATTGCatgat < 1:1171109/69‑1 (MQ=255) tCCACAGCCAGAAAAGCGTGCCCAGAATGCAGGAAAGTGTGGTTCGCCAGAAGTTAACCATTGCatgat < 1:2595072/69‑1 (MQ=255) tCCACAGCCAGAAAAGCGTGCCCAGAATGCAGGAAAGTGTGGTTCGCCAGAAGTTAACCATTGCatgat < 1:2024745/69‑1 (MQ=255) tCCACAGCCAGAAAAGCGTGCCCAGAATGCAGGAAAGTGTGGTTCGCCAGAAGTTAACCATTGCatgat < 1:2211827/69‑1 (MQ=255) tCCACAGCCAGAAAAGCGTGCCCAGAATGCAGGAAAGTGTGGTTCGCCAGAAGTTAACCATTGCatgat < 1:1457303/69‑1 (MQ=255) tCCACAGCCAGAAAAGCGTGCCCAGAATGCAGGAAAGTGTGGTTCGCCAGAAGTTAACCATTGCatgat < 1:296758/69‑1 (MQ=255) aaGCGTGCCCAGAATGCAGGAAAGTGTGGTTCGCCAGAAGTTAACCATTGCATGATGACGTTCGGCTGa < 1:594218/69‑1 (MQ=255) aaGCGTGCCCAGAATGCAGGAAAGTGTGGTTCGCCAGAAGTTAACCATTGCATGATGACGTTCGGCTGa < 1:3222041/69‑1 (MQ=255) aaGCGTGCCCAGAATGCAGGAAAGTGTGGTTCGCCAGAAGTTAACCATTGCATGATGACGTTCGGCTGa < 1:1255196/69‑1 (MQ=255) aaGCGTGCCCAGAATGCAGGAAAGTGGGGTTCGCCAGAAGTTAACCATTGCATGATGACGTTCGGCTGa < 1:307999/69‑1 (MQ=255) aGCGTGCCCAGAATGCAGGAAAGTGTGGTTCGCCAGAAGTTAACCATTGCATGATGACGTTCGGCTGa < 1:2099078/68‑1 (MQ=255) aGCGTGCCCAGAATGCAGGAAAGTGTGGTTCGCCAGAAGTTAACCATTGCATGATGACGTTCGGCTGa < 1:2611786/68‑1 (MQ=255) aGCGTGCCCAGAATGCAGGAAAGTGTGGTTCGCCAGAAGTTAACCATTGCATGATGACGTTCGGCTGAc < 1:2045122/69‑1 (MQ=255) aGCGTGCCCAGAATGCAGGAAAGTGTGGTTCGCCAGAAGTTAACCATTGCATGATGACGTTCGGCTGAc < 1:3041642/69‑1 (MQ=255) aGCGTGCCCAGAATGCAGGAAAGTGTGGTTCGCCAGAAGTTAACCATTGCATGATGACGTTCGGCTGAc < 1:1585755/69‑1 (MQ=255) aGCGTGCCCAGAATGCAGGAAAGTGTGGTTCGCCAGAAGTTAACCATTGCATGATGACGTTCGGCTGAc < 1:2634116/69‑1 (MQ=255) aGCGTGCCCAGAATGCAGGAAAGTGTGGTTCGCCAGAAGTTAACCATTGCATGATGACGTTCGGCTGAc < 1:1157044/69‑1 (MQ=255) aGCGTGCACAGAATGCAGGAAAGTGTGGTTCGCCAGAAGTTAACCATTGCATGATGACGTTCGGCTGAc < 1:3011356/69‑1 (MQ=255) aGCGGGCCCAGAATGCAGGAAAGTGTGGTTCGCCAGAAGTTAACCATTGCATGATGACGTTCGGCTGAc < 1:3039710/69‑1 (MQ=255) gCGTGCCCATAATGCAGGAAAGTGTGGTTCGCCAGAAGTTAACCATTGCATGATGACGTTCGGCTGAc < 1:3036420/68‑1 (MQ=255) cGTGCCCAGAATGCAAGAAAGTGTGGTTCGCCAGAAGTTAACCATTGCATGATGACGTTCGGCTGActc < 1:63149/69‑1 (MQ=255) cGTGCCCAGAATGCAAGAAAGTGTGGTTCGCCAGAAGTTAACCATTGCATGATGACGTTCGGCTGActc < 1:34370/69‑1 (MQ=255) cGTGCCCAGAATGCAAGAAAGTGTGGTTCGCCAGAAGTTAACCATTGCATGATGACGTTCGGCTGActc < 1:839958/69‑1 (MQ=255) cGTGCCCAGAATGCAAGAAAGTGTGGTTCGCCAGAAGTTAACCATTGCATGATGACGTTCGGCTGActc < 1:1718703/69‑1 (MQ=255) cGTGCCCAGAATGCAAGAAAGTGTGGTTCGCCAGAAGTTAACCATTGCATGATGACGTTCGGCTGActc < 1:3161486/69‑1 (MQ=255) cGTGCCCAGAATGCAAGAAAGTGTGGTTCGCCAGAAGTTAACCATTGCATGATGACGTTCGGCTGActc < 1:170882/69‑1 (MQ=255) cGTGCCCAGAATGCAAGAAAGTGTGGTTCGCCAGAAGTTAACCATTGCATGATGACGTTCGGCTGActc < 1:1199626/69‑1 (MQ=255) cGTGCCCAGAATGCAAGAAAGTGTGGTTCGCCAGAAGTTAACCATTGCATGATGACGTTCGGCTGActc < 1:2957278/69‑1 (MQ=255) cGTGCCCAGAATGCAAGAAAGTGTGGTTCGCCAGAAGTTAACCATTGCATGATGACGTTCGGCTGActc < 1:2690815/69‑1 (MQ=255) cGTGCCCAGAATGCAAGAAAGTGTGGTTCGCCAGAAGTTAACCATTGCATGATGACGTTCGGCTGActc < 1:2876835/69‑1 (MQ=255) cGTGCCCAGAATGCAAGAAAGTGTGGTTCGCCAGAAGTTAACCATTGCATGATGACGTTCGGCTGActc < 1:2897177/69‑1 (MQ=255) cGTGCCCAGAATGCAAGAAAGTGTGGTTCGCCAGAAGTTAACCATTGCATGATGACGTTCGGCTGActc < 1:1394349/69‑1 (MQ=255) cGTGCCCAGAATGCAAGAAAGTGTGGTTCGCCAGAAGTTAACCATTGCATGATGACGTTCGGCTGActc < 1:2908562/69‑1 (MQ=255) cGTGCCCAGAATGCAAGAAAGTGTGATTCGCCAGAAGTTAACCATTGCATGATGACGTTCGGCTGActc < 1:2050200/69‑1 (MQ=255) tGCCCAGAATGCAGGAAAGTGTGGTTCACCAGAAGTTAACCATTGCATGATGACGTTCGGCTGACTCTa < 1:1142977/69‑1 (MQ=255) gCCCAGAATGCAGGAAAGTGTGGTTCACCAGAAGTTAACCATTGCATGATGACGTTCGGCTGACTCTa < 1:845248/68‑1 (MQ=255) aGAATGCAAGAAAGTGTGGTTCGCCAGAAGTTAACCATTGCATGATGACGTTCGGCTGActc < 1:1528945/62‑1 (MQ=255) gAATGCAAGAAAGTGTGGTTCGCCAGAAGTTAACCATTTAATGATTACGTTCGGCTGActc < 1:509340/61‑1 (MQ=255) gAATGCAAGAAAGTGTGGTTCGCCAGAAGTTAACCATTGCATGATGACGTTCGGCTGActc < 1:2686939/61‑1 (MQ=255) cAGGAAAGTGTGGTTCGCCAGAAGTTAACCATTGCATGATGACGTTCGGCTGACTCTACTTTTACTTcc > 1:3130182/1‑69 (MQ=255) cAGGAAAGTGTGGTTCGCCAGAAGTTAACCATTGCATGATGACGTTCGGCTGACTCTACTTTTACTTcc > 1:1247712/1‑69 (MQ=255) cAGGAAAGTGTGGTTCGCCAGAAGTTAACCATTGCATGATGACGTTCGGCTGACTCTACTTTTACTTcc > 1:2823040/1‑69 (MQ=255) cAGGAAAGTGTGGTTCGCCAGAAGTTAACCATTGCATGATGACGTTCGGCTGACTCTACTTTTACTTcc > 1:2018635/1‑69 (MQ=255) cAGGAAAGTGTGGTTCGCCAGAAGTTAACCATTGCATGATGACGTTCGGCTGACTCTACTTTTACTTcc > 1:1030952/1‑69 (MQ=255) cAGGAAAGTGTGGTTCGCCAGAAGTTAACCATTGCATGATGACGTTCGGCTGACTCTACTTTTACTTc > 1:2685927/1‑68 (MQ=255) ggAAAGTGTGGTTCGCCAGAAGTTAACCATTGCATGATGACGTTCGGCTGACTCTACTTTTACTTCCgg < 1:16162/69‑1 (MQ=255) | CATCACCATTGCACCACTGCCGGAAGTCCAGCCCGTCCACAGCCAGAAAAGCGTGCCCAGAATGCAGGAAAGTGTGGTTCGCCAGAAGTTAACCATTGCATGATGACGTTCGGCTGACTCTACTTTTACTTCCGG > minE/2202313‑2202447 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |