| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,203,788 | C→A | 81.7% | P207P (CCG→CCT) | aaeA ← | p‑hydroxybenzoic acid efflux system component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,203,788 | 0 | C | A | 81.7% | 118.0 / 22.0 | 71 | P207P (CCG→CCT) | aaeA | p‑hydroxybenzoic acid efflux system component |
| Reads supporting (aligned to +/- strand): ref base C (5/8); new base A (33/25); total (38/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.57e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01 | |||||||||||
ACACTATCAACAGTCCCTTTCAGCACTTTGTTACTGCCAAGCGGCGTGATCTCTGCACGATACCCCGGACGCACCCCTTCCAGCTTAGTTTCTTCCATATAGGCCAGTACATAGAAGGAGTTCTGTTTC > minE/2203723‑2203851 | acacTATCAATAGTCCCTTTCAGCACTTTGTTACTGCCAAGCGGCGTGATCTCCGCACGATACCCAggg > 1:2368976/1‑68 (MQ=255)acacTATCAACAGTCCCTTTCAGCACTTTGTTACTGCCAAGCGGCGTGATCTCCGCACGATACCCAggg > 1:990030/1‑68 (MQ=255)acacTATCAACAGTCCCTTTCAGCACTTTGTTACTGCCAAGCGGCGTGATCTCCGCACGATACCCAggg > 1:1746253/1‑68 (MQ=255)acacTATCAACAGTCCCTTTCAGCACTTTGTTACTGCCAAGCGGCGTGATCTCCGCACGATACCCAggg > 1:3051332/1‑68 (MQ=255)acacTATCAACAGTCCCTTTCAGCACTTTGTTACTGCCAAGCGGCGTGATCTCCGCACGATACCCAggg > 1:1647840/1‑68 (MQ=255)acacTATCAACAGTCCCTTTCAGCACTTTGTTACTGCCAAGCGGCGTGATCTCCGCACGATACCCAggg > 1:14608/1‑68 (MQ=255)acacTATCAACAGTCCCTTTCAGCACTTTGTTACTGCCAAGCGGCGTGATCTCCGCACGATACCCAggg > 1:58528/1‑68 (MQ=255)acacTATCAACAGTCCCTTTCAGCACTTTGTTACTGCCAAGCGGCGTGATCTCCGCACGATACCCAggg > 1:1092337/1‑68 (MQ=255)acacTATCAACAGTCCCTTTCAGCACTTTGTTACTGCCAAGCGGCGTGATCTCCGCACGATACCCAggg > 1:689237/1‑68 (MQ=255) aTCAACAGTCCCTTTCAGCACTTTGTTACTGCCAAGCGGCGTGATCTCTGCACGATACCCCGGACGc > 1:368113/1‑67 (MQ=255) aTCAACAGTCCCTTTCAGCACTTTGTTACTGCCAAGCGGCGTGATCTCTGCACGATACCCCGGACGc > 1:1272594/1‑67 (MQ=255) aTCAACAGTCCCTTTCAGCACTTTGTTACTGCCAAGCGGCGTGATCTCTGCACGATACCCCGGACGc > 1:1794730/1‑67 (MQ=255) aTCAACAGTCCCTTTCAGCACTTTGTTACTGCCAAGCGGCGTGATCTCTGCACGATACCCCGGACGc > 1:1730831/1‑67 (MQ=255) cAGTCCCTTTCAGCACTTTGTTACTGCCAAGCGGCGTGATCTCCGCACGATACCCAGGGCGCACCCCt < 1:508569/68‑1 (MQ=255) cAGTCCCTTTCAGCACTTTGTTACTGCCAAGCGGCGTGATCTCCGCACGATACCCAGGGCGCACCCCt < 1:2586016/68‑1 (MQ=255) cAGTCCCTTTCAGCACTTTGTTACTGCCAAGCGGCGTGATCTCCGCACGATACCCAGGGCGCACCCCt < 1:2716515/68‑1 (MQ=255) cAGTCCCTTTCAGCACTTTGTTACTGCCAAGCGGCGTGATCTCCGCACGATACCCAGGGCGCACCCCt < 1:1906910/68‑1 (MQ=255) cAGTCCCTTTCAGCACTTTGTTACTGCCAAGCGGCGTGATCTCCGCACGATACCCAGGGCGCACCCCt < 1:1846626/68‑1 (MQ=255) cAGTCCCTTTCAGCACTTTGTTACTGCCAAGCGGCGTGATCTCCGCACGATACCCAGGGCGCACCCCt < 1:1800230/68‑1 (MQ=255) cAGTCCCTTTCAGCACTTTGTTACTGCCAAGCGGCGTGATCTCCGCACGATACCCAGGGCGCACCCCt < 1:1799692/68‑1 (MQ=255) cAGTCCCTTTCAGCACTTTGTTACTGCCAAGCGGCGTGATCTCCGCACGATACCCAGGGCGCACCCCt < 1:1099978/68‑1 (MQ=255) cAGTCCCTTTCAGCACTTTGTTACTGCCAAGCGGCGTGATCTCCGCACGATACCCAGGGCGCACCCCt < 1:1602812/68‑1 (MQ=255) cAGTCCCTTTCAGCACTTTGTTACTGCCAAGCGGCGTGATCTCCGCACGATACCCAGGGCGCACCCCt < 1:1583028/68‑1 (MQ=255) cAGTCCCTTTCAGCACTTTGTTACTGCCAAGCGGCGTGATCTCCGCACGATACCCAGGGCGCACCCCt < 1:153517/68‑1 (MQ=255) cAGTCCCTTTCAGCACTTTGTTACTGCCAAGCGGCGTGATCTCCGCACGATACCCAGGGCGCACCCCt < 1:3399794/68‑1 (MQ=255) tttGTTACTGCCAAGCGGCGTGATCTCTGCACGTTACCCCGGACGCACCCCTTCCAGCTTAGTttcttc < 1:2062339/69‑1 (MQ=255) tttGTTACTGCCAAGCGGCGTGATCTCTGCACGATACCCCGGACGCACCCCTTCCAGCTTAGTttcttc < 1:2812274/69‑1 (MQ=255) ttACTGCCAAGCGGCGTGATCTCTGCACGATACCCCGGACGCACCCCTTCCAGCTTAGTTTCTTCCa < 1:1600577/67‑1 (MQ=255) ttACTGCCAAGCGGCGTGATCTCTGCACGATACCCCGGACGCACCCCTTCCAGCTTAGTTTCTTCCa < 1:2959027/67‑1 (MQ=255) ttACTGCCAAGCGGCGTGATCTCTGCACGATACCCCGGACGCACCCCTTCCAGCTTAGTTTCTTCCa < 1:585671/67‑1 (MQ=255) ttACTGCCAAGCGGCGTGATCTCTGCACGATACCCCGGACGCACCCCTTCCAGCTTAGTTTCTTCCa < 1:2849249/67‑1 (MQ=255) ttACTGCCAAGCGGCGTGATCTCTGCACGATACCCCGGACGCACCCCTTCCAGCTTAGTTTCTTCCa < 1:1241754/67‑1 (MQ=255) tACTGCCAAGCGGCGTGATCTCTGCACGATACCCCGGACGCACCCCTTCCAGCTTAGTTTCTTCCa < 1:1254944/66‑1 (MQ=255) cTGCCAAGCGGCGTGATCTCCGCACGATACCCAGGGCGCCCCCCTTCCAGCTTAGTTTCTTCCata > 1:3066180/1‑66 (MQ=255) cTGCCAAGCGGCGTGATCTCCGCACGATACCCAGGGCGCACCCCt > 1:105882/1‑45 (MQ=38) cTGCCAAGCGGCGTGATCTCCGCACGATACCCAGGGCGCACCCCTTCCAGCTTAGTTTCTTCCata > 1:229633/1‑66 (MQ=255) cTGCCAAGCGGCGTGATCTCCGCACGATACCCAGGGCGCACCCCTTCCAGCTTAGTTTCTTCCata > 1:998234/1‑66 (MQ=255) cTGCCAAGCGGCGTGATCTCCGCACGATACCCAGGGCGCACCCCTTCCAGCTTAGTTTCTTCCata > 1:1007569/1‑66 (MQ=255) cTGCCAAGCGGCGTGATCTCCGCACGATACCCAGGGCGCACCCCTTCCAGCTTAGTTTCTTCCata > 1:971643/1‑66 (MQ=255) cTGCCAAGCGGCGTGATCTCCGCACGATACCCAGGGCGCACCCCTTCCAGCTTAGTTTCTTCCata > 1:1108314/1‑66 (MQ=255) cTGCCAAGCGGCGTGATCTCCGCACGATACCCAGGGCGCACCCCTTCCAGCTTAGTTTCTTCCata > 1:568058/1‑66 (MQ=255) cTGCCAAGCGGCGTGATCTCCGCACGATACCCAGGGCGCACCCCTTCCAGCTTAGTTTCTTCCata > 1:514251/1‑66 (MQ=255) cTGCCAAGCGGCGTGATCTCCGCACGATACCCAGGGCGCACCCCTTCCAGCTTAGTTTCTTCCata > 1:118479/1‑66 (MQ=255) cTGCCAAGCGGCGTGATCTCCGCACGATACCCAGGGCGCACCCCTTCCAGCTTAGTTTCTTCCata > 1:47851/1‑66 (MQ=255) cTGCCAAGCGGCGTGATCTCCGCACGATACCCAGGGCGCACCCCTTCCAGCTTAGTTTCTTCCata > 1:464206/1‑66 (MQ=255) cTGCCAAGCGGCGTGATCTCCGCACGATACCCAGGGCGCACCCCTTCCAGCTTAGTTTCTTCCata > 1:375299/1‑66 (MQ=255) cTGCCAAGCGGCGTGATCTCCGCACGATACCCAGGGCGCACCCCTTCCAGCTTAGTTTCTTCCata > 1:1261897/1‑66 (MQ=255) cTGCCAAGCGGCGTGATCTCCGCACGATACCCAGGGCGCACCCCTTCCAGCTTAGTTTCTTCCata > 1:365302/1‑66 (MQ=255) cTGCCAAGCGGCGTGATCTCCGCACGATACCCAGGGCGCACCCCTTCCAGCTTAGTTTCTTCCata > 1:1379276/1‑66 (MQ=255) cTGCCAAGCGGCGTGATCTCCGCACGATACCCAGGGCGCACCCCTTCCAGCTTAGTTTCTTCCata > 1:3068435/1‑66 (MQ=255) cTGCCAAGCGGCGTGATCTCCGCACGATACCCAGGGCGCACCCCTTCCAGCTTAGTTTCTTCCata > 1:1458196/1‑66 (MQ=255) cTGCCAAGCGGCGTGATCTCCGCACGATACCCAGGGCGCACCCCTTCCAGCTTAGTTTCTTCCata > 1:2181733/1‑66 (MQ=255) cTGCCAAGCGGCGTGATCTCCGCACGATACCCAGGGCGCACCCCTTCCAGCTTAGTTTCTTCCata > 1:2469986/1‑66 (MQ=255) cTGCCAAGCGGCGTGATCTCCGCACGATACCCAGGGCGCACCCCTTCCAGCTTAGTTTCTTCCata > 1:2804025/1‑66 (MQ=255) cTGCCAAGCGGCGTGATCTCCGCACGATACCCAGGGCGCACCCCTTCCAGCTTAGTTTCTTCCata > 1:2640797/1‑66 (MQ=255) cTGCCAAGCGGCGTGATCTCCGCACGATACCCAGGGCGCACCCCTTCCAGCTTAGTTTCTTCCata > 1:2625635/1‑66 (MQ=255) cTGCCAAGCGGCGTGATCTCCGCACGATACCCAGGGCGCACCCCTTCCAGCTTAGTTTCTTCCat > 1:1114209/1‑65 (MQ=255) aTCTCTGCACGATACCCCGGACGCACCCCTTCCAGCTTAGTTTCTTCCATATAGGCCAGTACATAGa > 1:2718967/1‑67 (MQ=255) ctccGCACGATACCCAGGGCGCACCCCTTCCAGCTTAGTTTCTTCCATATAGGCCAGTACATAGAAgg < 1:2085181/68‑1 (MQ=255) ctccGCACGATACCCAGGGCGCACCCCTTCCAGCTTAGTTTCTTCCATATAGGCCAGTACATAGAAgg < 1:2177981/68‑1 (MQ=255) ctccGCACGATACCCAGGGCGCACCCCTTCCAGCTTAGTTTCTTCCATATAGGCCAGTACATAGAAgg < 1:2582768/68‑1 (MQ=255) ctccGCACGATACCCAGGGCGCACCCCTTCCAGCTTAGTTTCTTCCATATAGGCCAGTACATAGAAgg < 1:1118419/68‑1 (MQ=255) ctccGCACGATACCCAGGGCGCACCCCTTCCAGCTTAGTTTCTTCCATATAGGCCAGTACATAGAAgg < 1:2151997/68‑1 (MQ=255) ctccGCACGATACCCAGGGCGCACCCCTTCCAGCTTAGTTTCTTCCATATAGGCCAGTACATAGAAgg < 1:2615564/68‑1 (MQ=255) ctccGCACGATACCCAGGGCGCACCCCTTCCAGCTTAGTTTCTTCCATATAGGCCAGTACATAGAAgg < 1:261790/68‑1 (MQ=255) ctccGCACGATACCCAGGGCGCACCCCTTCCAGCTTAGTTTCTTCCATATAGGCCAGTACATAGAAgg < 1:2881522/68‑1 (MQ=255) ctccGCACGATACCCAGGGCGCACCCCTTCCAGCTTAGTTTCTTCCATATAGGCCAGTACATAGAAgg < 1:3354799/68‑1 (MQ=255) ctccGCACGATACCCAGGGCGCACCCCTTCCAGCTTAGTTTCTTCCATATAGGCCAGTACATAGAAgg < 1:333694/68‑1 (MQ=255) ctccGCACGATACCCAGGGCGCACCCCTTCCAGCTTAGTTTCTTCCATATAGGCCAGTACATAGAAgg < 1:179602/68‑1 (MQ=255) ctccGCACGATACCCAGGGCGCACCCCTTCCAGCTTAGTTTCTTCCATATAGGCCAGTACATAGAAgg < 1:2890130/68‑1 (MQ=255) gctcGATACCCAGGGCGCACCCCTTCCAGCTTAGTTTCTTCCATATAGGCCAGTACATAGAAgg < 1:1706388/61‑1 (MQ=255) ccccGGACGCACCCCTTCCAGCTTAGTTTCTTCCATATAGGCCAGTACATAGAAGGAGTTCTGTTTc > 1:273780/1‑67 (MQ=255) ccccGGACGCACCCCTTCCAGCTTAGTTTCTTCCATATAGGCCAGTACATAGAAGGAGTTCTGTTTc > 1:1870110/1‑67 (MQ=255) ccccGGACGCACCCCTTCCAGCTTAGTTTCTTCCATATAGGCCAGTACATAGAAGGAGTTCTGTTTc > 1:1855556/1‑67 (MQ=255) | ACACTATCAACAGTCCCTTTCAGCACTTTGTTACTGCCAAGCGGCGTGATCTCTGCACGATACCCCGGACGCACCCCTTCCAGCTTAGTTTCTTCCATATAGGCCAGTACATAGAAGGAGTTCTGTTTC > minE/2203723‑2203851 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |