| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,205,665 | G→A | 60.3% | L288L (CTG→CTA) | aaeR → | predicted DNA‑binding transcriptional regulator, efflux system |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,205,665 | 0 | G | A | 60.3% | 27.9 / 45.5 | 68 | L288L (CTG→CTA) | aaeR | predicted DNA‑binding transcriptional regulator, efflux system |
| Reads supporting (aligned to +/- strand): ref base G (23/4); new base A (24/17); total (47/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.09e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.25e-01 | |||||||||||
TTATGCGTTATATACCGAAAAAGATAAGCTGCCGCTGAAGGTACAGGTCGTGATCAACTCGCTGACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAG > minE/2205602‑2205723 | ttATGCGTTATATACCGAAAAAGATAAGCTGCCGCTGAAGGTACAGGTCGTGATCAACTCGCTGACgg > 1:2542297/1‑68 (MQ=255) ttATATACCGAAAAAGATAAGCTGCCGCTGAAGGTACAGGTCGTGATCAACTCGCTGACGGATTAtttt > 1:2113741/1‑69 (MQ=255) ttATATACCGAAAAAGATAAGCTGCCGCTGAAGGTACAAGTCGTGATCAACTCGCTAACGGATTAttt < 1:3049883/68‑1 (MQ=255) ttATATACCGAAAAAGATAAGCTGCCGCTGAAGGTACAAGTCGTGATCAACTCGCTAACGGATTAttt < 1:2161450/68‑1 (MQ=255) ttATATACCGAAAAAGATAAGCTGCCGCTGAAGGTACAAGTCGTGATCAACTCGCTAACGGATTAttt < 1:2391545/68‑1 (MQ=255) ttATATACCGAAAAAGATAAGCTGCCGCTGAAGGTACAAGTCGTGATCAACTCGCTAACGGATTAttt < 1:1115197/68‑1 (MQ=255) ttATATACCGAAAAAGATAAGCTGCCGCTGAAGGTACAAGTCGTGATCAACTCGCTAACGGATTAttt < 1:1796060/68‑1 (MQ=255) ttATATACCGAAAAAGATAAGCTGCCGCTGAAGGTACAAGTCGTGATCAACTCGCTAACGGATTAttt < 1:1701122/68‑1 (MQ=255) ttATATACCGAAAAAGATAAGCTGCCGCTGAAGGTACAAGTCGTGATCAACTCGCTAACGGATTAttt < 1:2682360/68‑1 (MQ=255) ttATATACCGAAAAAGATAAGCTGCCGCTGAAGGTACAAGTCGTGATCAACTCGCTAACGGATTAttt < 1:1623813/68‑1 (MQ=255) ttATATACCGAAAAAGATAAGCTGCCGCTGAAGGTACAAGTCGTGATCAACTCGCTAACGGATTAttt < 1:3056231/68‑1 (MQ=255) ttATATACCGAAAAAGATAAGCTGCCGCTGAAGGTACAAGTCGTGATCAACTCGCTAACGGATTAttt < 1:3288187/68‑1 (MQ=255) ttATATACCGAAAAAGATAAGCTGCCGCTGAAGGTACAAGTCGTGATCAACTCGCTAACGGATTAttt < 1:459924/68‑1 (MQ=255) ttATATACCGAAAAAGATAAGCTGCCGCTGAAGGTACAAGTCGTGATCAACTCGCTAACGGATTAttt < 1:133215/68‑1 (MQ=255) ttATATACCGAAAAAGATAAGCTGCCGCTGAAGGTACAAGTCGTGATCAACTCGCTAACGGATTAttt < 1:557795/68‑1 (MQ=255) ttATATACCGAAAAAGATAAGCTGCCGCTGAAGGTACAAGTCGTGATCAACTCGCTAACGGATTAttt < 1:769800/68‑1 (MQ=255) ttATATACCGAAAAAGATAAGCTGCCGCTGAAGGTACAAGTCGTGATCAACTCGCTAACGGATTAttt < 1:780912/68‑1 (MQ=255) ataCCGAAAAAGATAAGCTGCCGCTGAAGGTACAGGTCGTGATCAACTCGCTGACGGATTATTttgttg > 1:2940687/1‑69 (MQ=255) ataCCGAAAAAGATAAGCTGCCGCTGAAGGTACAGGTCGTGATCAACTCGCTGACGGATTATTttgttg > 1:652242/1‑69 (MQ=255) ataCCGAAAAAGATAAGCTGCCGCTGAAGGTACAGGTCGTGATCAACTCGCTGACGGATTATTttgttg > 1:995340/1‑69 (MQ=255) aGCTGCCGCTGAAGGTACAAGTCGTGATCAACTCGCTAACGGATTAttt < 1:1380274/49‑1 (MQ=39) ccGCTGAAGGTACAAGTCGTGATCAACTCGCTAACGGATTATTTTGTTGAGGTCGGTaaa > 1:2534115/1‑60 (MQ=255) ccGCTGAAGGTACAAGTCGTGATCAACTCGCTAACGGATTATTTTGTTGAGGTCGGTaaa > 1:2062838/1‑60 (MQ=255) ccGCTGAAGGTACAAGTCGTGATCAACTCGCTAACGGATTATTTTGTTGAGGTCGGTaa > 1:3036945/1‑59 (MQ=255) ccGCTGAAGGTACAAGTCGTGATCAACTCGCTAACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCa > 1:2111201/1‑68 (MQ=255) ccGCTGAAGGTACAAGTCGTGATCAACTCGCTAACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCa > 1:1225389/1‑68 (MQ=255) ccGCTGAAGGTACAAGTCGTGATCAACTCGCTAACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCa > 1:769777/1‑68 (MQ=255) ccGCTGAAGGTACAAGTCGTGATCAACTCGCTAACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCa > 1:3401716/1‑68 (MQ=255) ccGCTGAAGGTACAAGTCGTGATCAACTCGCTAACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCa > 1:3344055/1‑68 (MQ=255) ccGCTGAAGGTACAAGTCGTGATCAACTCGCTAACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCa > 1:3150401/1‑68 (MQ=255) ccGCTGAAGGTACAAGTCGTGATCAACTCGCTAACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCa > 1:311997/1‑68 (MQ=255) ccGCTGAAGGTACAAGTCGTGATCAACTCGCTAACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCa > 1:1591827/1‑68 (MQ=255) ccGCTGAAGGTACAAGTCGTGATCAACTCGCTAACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCa > 1:3009734/1‑68 (MQ=255) ccGCTGAAGGTACAAGTCGTGATCAACTCGCTAACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCa > 1:1628710/1‑68 (MQ=255) ccGCTGAAGGTACAAGTCGTGATCAACTCGCTAACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCa > 1:2842478/1‑68 (MQ=255) ccGCTGAAGGTACAAGTCGTGATCAACTCGCTAACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCa > 1:2838633/1‑68 (MQ=255) ccGCTGAAGGTACAAGTCGTGATCAACTCGCTAACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCa > 1:1637164/1‑68 (MQ=255) ccGCTGAAGGTACAAGTCGTGATCAACTCGCTAACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCa > 1:2659662/1‑68 (MQ=255) ccGCTGAAGGTACAAGTCGTGATCAACTCGCTAACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCa > 1:1745611/1‑68 (MQ=255) ccGCTGAAGGTACAAGTCGTGATCAACTCGCTAACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCa > 1:183268/1‑68 (MQ=255) ccGCTGAAGGTACAAGTCGTGATCAACTCGCTAACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCa > 1:2093583/1‑68 (MQ=255) ccGCTGAAGGTACAAGTCGTGATCAACTCGCTAACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCa > 1:2133481/1‑68 (MQ=255) ccGCTGAAGGTACAAGTCGTGATCAACTCGCTAACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCa > 1:1962282/1‑68 (MQ=255) ccGCTGAAGGTACAAGTCGTGATCAACTCGCTAACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCa > 1:2452045/1‑68 (MQ=255) ccGCTGAAGGTACAAGTCGTGATCAACTCGCTAACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCa > 1:2394464/1‑68 (MQ=255) gAAGGTACAAGTCGTGATCAACTCGCTAACGGATTAttt < 1:2487015/39‑1 (MQ=37) aCAGGTCGTGATCAACTCGCTGACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCAGgaga > 1:2386937/1‑62 (MQ=255) aCAGGTCGTGATCAACTCGCTGACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCAGGAGATGCACgg > 1:345469/1‑69 (MQ=255) ggTCGTGATCAACTCGCTGACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCAGGAGATg > 1:2747530/1‑61 (MQ=255) ggTCGTGATCAACTCGCTGACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCAGGAGATGCACGGgcg > 1:2844877/1‑69 (MQ=255) gTCGTGATCAACTCGCTGACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCAGga > 1:3095117/1‑56 (MQ=255) gTCGTGATCAACTCGCTGACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCAGGAGATGCACGGgcgc > 1:1314872/1‑69 (MQ=255) gTCGTGATCAACTCGCTGACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCAGGAGATGCACGGgcgc > 1:3303934/1‑69 (MQ=255) aTCAACTCGCTGACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGa > 1:3267014/1‑67 (MQ=255) cAACTCGCTGACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCAGga > 1:1151198/1‑48 (MQ=255) cAACTCGCTGACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCAGGAGCTGCACGGGCGCGGGAAag > 1:595515/1‑68 (MQ=255) cAACTCGCTGACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCAGGAGAt > 1:2525340/1‑51 (MQ=255) cAACTCGCTGACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCAGGAGATg > 1:1388167/1‑52 (MQ=255) cAACTCGCTGACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAag > 1:3300612/1‑68 (MQ=255) cAACTCGCTGACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAag > 1:2228791/1‑68 (MQ=255) cAACTCGCTGACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAag > 1:2000086/1‑68 (MQ=255) cAACTCGCTGACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAag > 1:340852/1‑68 (MQ=255) cAACTCGCTGACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAag < 1:1590907/68‑1 (MQ=255) cAACTCGCTGACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAag > 1:1372610/1‑68 (MQ=255) cAACTCGCTGACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAag < 1:2963446/68‑1 (MQ=255) cAACTCGCTGACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAag < 1:1300662/68‑1 (MQ=255) cAACTCGCTGACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAag > 1:1293206/1‑68 (MQ=255) cAACTCGCTGACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAag < 1:2869948/68‑1 (MQ=255) | TTATGCGTTATATACCGAAAAAGATAAGCTGCCGCTGAAGGTACAGGTCGTGATCAACTCGCTGACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAG > minE/2205602‑2205723 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |