| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,209,246 | C→T | 58.2% | L765L (TTG→TTA) | yhdP ← | conserved membrane protein, predicted transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,209,246 | 0 | C | T | 58.2% | 19.4 / 48.6 | 67 | L765L (TTG→TTA) | yhdP | conserved membrane protein, predicted transporter |
| Reads supporting (aligned to +/- strand): ref base C (18/10); new base T (18/21); total (36/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.14e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCACCAGCATGATAAGGCAGATCAATGCCCACTTTACCATCCCACGCCACGCTGCCACTCAATGCTTCGTTCACCGCTTCAGGCAGAACGCCGGTTTTCGCCGGTTGCCAGTTACCGTTGAGGTTTAC > minE/2209187‑2209314 | gCACCAGCATTATAAGGCAGATCAATGCCCACTTTACCATCCCACGCCACGCTGCCACTCAATGCTTCg > 1:488045/1‑69 (MQ=255)gCACCAGCATGATAAGGCAGATCAATGCCCACTTTACCATCCCACGCCACGCTGCCACTCAATGCTTCg > 1:1600220/1‑69 (MQ=255)gCACCAGCATGATAAGGCAGATCAATGCCCACTTTACCATCCCACGCCACGCTGCCACTCAATGCTTCg > 1:443049/1‑69 (MQ=255) gCAGCTCAATGCCCACTTTACCATCCCACGGCACGCTGCCACTTAATGCTTCGTTCACCGCTGCAGGCa < 1:375781/69‑1 (MQ=255) gCAGCTCAATGCCCACTTTACCATCCCACGCCACGCTGCCACTTAATGCTTCGTTCACCGCTGCAGGct < 1:1084653/69‑2 (MQ=255) gCAGCTCAATGCCCACTTTACCATCCCACGCCACGCTGCCACTTAATGCTTCGTTCACCGCTGCAGGCa < 1:3078578/69‑1 (MQ=255) gCAGCTCAATGCCCACTTTACCATCCCACGCCACGCTGCCACTTAATGCTTCGTTCACCGCTGCAGGCa < 1:2979703/69‑1 (MQ=255) gCAGCTCAATGCCCACTTTACCATCCCACGCCACGCTGCCACTTAATGCTTCGTTCACCGCTGCAGGCa < 1:28359/69‑1 (MQ=255) gCAGCTCAATGCCCACTTTACCATCCCACGCCACGCTGCCACTTAATGCTTCGTTCACCGCTGCAGGCa < 1:2802799/69‑1 (MQ=255) gCAGCTCAATGCCCACTTTACCATCCCACGCCACGCTGCCACTTAATGCTTCGTTCACCGCTGCAGGCa < 1:3270562/69‑1 (MQ=255) gCAGCTCAATGCCCACTTTACCATCCCACGCCACGCTGCCACTTAATGCTTCGTTCACCGCTGCAGGCa < 1:2598161/69‑1 (MQ=255) gCAGCTCAATGCCCACTTTACCATCCCACGCCACGCTGCCACTTAATGCTTCGTTCACCGCTGCAGGCa < 1:2542226/69‑1 (MQ=255) gCAGCTCAATGCCCACTTTACCATCCCACGCCACGCTGCCACTTAATGCTTCGTTCACCGCTGCAGGCa < 1:524175/69‑1 (MQ=255) gCAGCTCAATGCCCACTTTACCATCCCACGCCACGCTGCCACTTAATGCTTCGTTCACCGCTGCAGGCa < 1:534064/69‑1 (MQ=255) gCAGCTCAATGCCCACTTTACCATCCCACGCCACGCTGCCACTTAATGCTTCGTTCACCGCTGCAGGCa < 1:2311846/69‑1 (MQ=255) gCAGCTCAATGCCCACTTTACCATCCCACGCCACGCTGCCACTTAATGCTTCGTTCACCGCTGCAGGCa < 1:2447542/69‑1 (MQ=255) gCAGCTCAATGCCCACTTTACCATCCCACGCCACGCTGCCACTTAATGCTTCGTTCACCGCTGCAGGCa < 1:2008535/69‑1 (MQ=255) gCAGCTCAATGCCCACTTTACCATCCCACGCCACGCTGCCACTTAATGCTTCGTTCACCGCTGCAGGCa < 1:1892009/69‑1 (MQ=255) gCAGCTCAATGCCCACTTTACCATCCCACGCCACGCTGCCACTTAATGCTTCGTTCACCGCTGCAGGCa < 1:1666195/69‑1 (MQ=255) gCAGCTCAATGCCCACTTTACCATCCCACGCCACGCTGCCACTTAATGCTTCGTTCACCGCTGCAGGCa < 1:1075382/69‑1 (MQ=255) gCAGCTCAATGCCCACTTTACCATCCCACGCCACGCTGCCACTTAATGCTTCGTTCACCGCTGCAGGCa < 1:1088711/69‑1 (MQ=255) gCAGCTCAATGCCCACTTTACCATCCCACGCCACGCTGCCACTTAATGCTTCGTTCACCGCTGCAGGCa < 1:1178068/69‑1 (MQ=255) gCAGCTCAATGCCCACTTTACCATCCCACGCCACGCTGCCACTTAATGCTTCGTTCACCGCTGCAGGCa < 1:1599798/69‑1 (MQ=255) gCAGCTCAATGCCCACTTTACCATCCCACGCCACGCTGCCACTTAATGCTTCGTTCACCGCTGCAGGCa < 1:1321568/69‑1 (MQ=255) tCAATGCCCACTTTACCATCCCACGCCACGCTGCCACTCAATGCTTCGtt > 1:2807689/1‑50 (MQ=255) tCAATGCCCACTTTACCATCCCACGCCACGCTGCCACTCAATGCTTCGTTCACCGCTTCAGGCAGAAc > 1:1513742/1‑68 (MQ=255) tCAATGCCCACTTTACCATCCCACGCCACGCTGCCACTCAATGCTTCGTTCACCGCTTCAGGCAGAACg > 1:3236865/1‑69 (MQ=255) tCAATGCCCACTTTACCATCCCACGCCACGCTGCCACTCAATGCTTCGTTCACCGCTTCAGGCAGAACg > 1:2888967/1‑69 (MQ=255) cccACTTTACCATCCCACGCCACGCTGCCACTCAATGCTTCGTTCACCGCTTCAGGCAGAACGcc < 1:2335615/65‑1 (MQ=255) ccACTTTACCATCCCACGCCACGCTGCCACTTAATGCTTCGTTCACCGCTGGAGGCAGAAAGCCGGttt > 1:100417/1‑69 (MQ=255) ccACTTTACCATCCCACGCCACGCTGCCACTTAATGCTTCGTTCACCGCTGCAGGCAGAAAGCCGGttt > 1:1458513/1‑69 (MQ=255) ccACTTTACCATCCCACGCCACGCTGCCACTTAATGCTTCGTTCACCGCTGCAGGCAGAAAGCCGGttt > 1:880731/1‑69 (MQ=255) ccACTTTACCATCCCACGCCACGCTGCCACTTAATGCTTCGTTCACCGCTGCAGGCAGAAAGCCGGttt > 1:356371/1‑69 (MQ=255) ccACTTTACCATCCCACGCCACGCTGCCACTTAATGCTTCGTTCACCGCTGCAGGCAGAAAGCCGGttt > 1:1082859/1‑69 (MQ=255) ccACTTTACCATCCCACGCCACGCTGCCACTTAATGCTTCGTTCACCGCTGCAGGCAGAAAGCCGGttt > 1:2042779/1‑69 (MQ=255) ccACTTTACCATCCCACGCCACGCTGCCACTTAATGCTTCGTTCACCGCTGCAGGCAGAAAGCCGGttt > 1:3125426/1‑69 (MQ=255) ccACTTTACCATCCCACGCCACGCTGCCACTTAATGCTTCGTTCACCGCTGCAGGCAGAAAGCCGGttt > 1:868701/1‑69 (MQ=255) ccACTTTACCATCCCACGCCACGCTGCCACTTAATGCTTCGTTCACCGCTGCAGGCAGAAAGCCGGttt > 1:458121/1‑69 (MQ=255) ccACTTTACCATCCCACGCCACGCTGCCACTTAATGCTTCGTTCACCGCTGCAGGCAGAAAGCCGGttt > 1:2971713/1‑69 (MQ=255) ccACTTTACCATCCCACGCCACGCTGCCACTTAATGCTTCGTTCACCGCTGCAGGCAGAAAGCCGGttt > 1:2878325/1‑69 (MQ=255) ccACTTTACCATCCCACGCCACGCTGCCACTTAATGCTTCGTTCACCGCTGCAGGCAGAAAGCCGGttt > 1:1191090/1‑69 (MQ=255) ccACTTTACCATCCCACGCCACGCTGCCACTTAATGCTTCGTTCACCGCTGCAGGCAGAAAGCCGGttt > 1:2401979/1‑69 (MQ=255) ccACTTTACCATCCCACGCCACGCTGCCACTTAATGCTTCGTTCACCGCTGCAGGCAGAAAGCCGGttt > 1:2804473/1‑69 (MQ=255) ccACTTTACCATCCCACGCCACGCTGCCACTTAATGCTTCGTTCACCGCTGCAGGCAGAAAGCCGGttt > 1:1284335/1‑69 (MQ=255) ccACTTTACCATCCCACGCCACGCTGCCACTTAATGCTTCGTTCACCGCTGCAGGCAGAAAGCCGGttt > 1:2750930/1‑69 (MQ=255) ccACTTTACCATCCCACGCCACGCTGCCACTTAATGCTTCGTTCACCGCTGCAGGCAGAAAGCCGGttt > 1:2493100/1‑69 (MQ=255) ccACTTTACCATCACACGCAACGCTGCCACTTAATGCTTCGTTCACCGCTGCAGGCAGAAAGCCGGttt > 1:3289621/1‑69 (MQ=255) tACCATCCCACGCCACGCTGCCACTCAATGCTTCGTTCACCGCTTCAGGCAGAACGCCGGTTTTCGCCg > 1:2856207/1‑69 (MQ=255) tACCATCCCACGCCACGCTGCCACTCAATGCTTCGTTCACCGCTTCAGGCAGAACGCCGGTTTTCGCCg > 1:2176793/1‑69 (MQ=255) tACCATCCCACGCCACGCTGCCACTCAATGCTTCGTTCACCGCTTCAGGCAGAACGCCGGTTTTCGCCg > 1:1317547/1‑69 (MQ=255) tACCATCCCACGCCACGCTGCCACTCAATGCTTCGTTCACCGCTTCAGGCAGAACGCCGGTTTTCGCCg > 1:265306/1‑69 (MQ=255) tACCATCCCACGCCACGCTGCCACTCAATGCTTCGTTCACCGCTTCAGGCAGAACGCCGGTTTTCGCCg > 1:135517/1‑69 (MQ=255) ccacgcTGCCACTCAATGCTTCGTTCACCGCTTCAGGCAGAACGCCGGTTTTCGCCGGTTGCCAGTTAc < 1:2978804/69‑1 (MQ=255) cTGCCACTCAATGCTTCGTTCACCGCTTCAGGCAg > 1:776439/1‑35 (MQ=255) cTGCCACTCAATGCTTCGTTCACCGCTTCAGGCAg > 1:1027064/1‑35 (MQ=255) cTGCCACTCAATGCTTCGTTCACCGCTTCAGGCAg > 1:957393/1‑35 (MQ=255) cTGCCACTCAATGCTTCGTTCACCGCTTCAGGCAg > 1:2113925/1‑35 (MQ=255) cTGCCACTCAATGCTTCGTTCACCGCTTCAGGCAg > 1:1885463/1‑35 (MQ=255) cTGCCACTCAATGCTTCGTTCACCGCTTCAGGCAg > 1:1664497/1‑35 (MQ=255) aCTCAATGCTTCGTTCACCGCTTCAGGCAGAACGCCGGTTTTCGCCGGTTGCCAGTTACCGTTGAGGtt < 1:354775/69‑1 (MQ=255) aCTCAATGCTTCGTTCACCGCTTCAGGCAGAACGCCGGTTTTCGCCGGTTGCCAGTTACCGTTGAGGtt < 1:3021856/69‑1 (MQ=255) aCTCAATGCTTCGTTCACCGCTTCAGGCAGAACGCCGGTTTTCGCCGGTTGCCAGTTACCGTTGAGGtt < 1:477970/69‑1 (MQ=255) aCTCAATGCTTCGTTCACCGCTTCAGGCAGAACGCCGGTTTTCGCCGGTTGCCAGTTACCGTTGAGGtt < 1:2502317/69‑1 (MQ=255) aCTCAATGCTTCGTTCACCGCTTCAGGCAGAACGCCGGTTTTCGCCGGTTGCCAGTTACCGTTGAGGtt < 1:2492850/69‑1 (MQ=255) aCTCAATGCTTCGTTCACCGCTTCAGGCAGAACGCCGGTTTTCGCCGGTTGCCAGTTACCGTTGAGGtt < 1:71090/69‑1 (MQ=255) aCTCAATGCTTCGTTCACCGCTTCAGGCAGAACGCCGGTTTTCGCCGGTTGCCAGTTACCGTTGAGGtt < 1:2267036/69‑1 (MQ=255) aCTCAATGCTTCGTTCACCGCTTCAGGCAGAACGCCGGTTTTCGCCGGTTGCCAGTTACCGTTGAGGtt < 1:974101/69‑1 (MQ=255) cAATGCTTCGTTCACCGCTTCAGGCAGAACGCCGGTTTTCGCCGGTTGCCAGTTACCGTTGAGGTTTAc > 1:2678636/1‑69 (MQ=255) | GCACCAGCATGATAAGGCAGATCAATGCCCACTTTACCATCCCACGCCACGCTGCCACTCAATGCTTCGTTCACCGCTTCAGGCAGAACGCCGGTTTTCGCCGGTTGCCAGTTACCGTTGAGGTTTAC > minE/2209187‑2209314 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |