Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,209,265 T→G 45.9% E759A (GAA→GCA)  yhdP ← conserved membrane protein, predicted transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,209,2650TG45.9% ‑1.2 / 100.2 85E759A (GAA→GCA) yhdPconserved membrane protein, predicted transporter
Reads supporting (aligned to +/- strand):  ref base T (24/22);  new base G (18/21);  total (42/43)
Fisher's exact test for biased strand distribution p-value = 6.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.01e-01

GCAGATCAATGCCCACTTTACCATCCCACGCCACGCTGCCACTCAATGCTTCGTTCACCGCTTCAGGCAGAACGCCGGTTTTCGCCGGTTGCCAGTTACCGTTGAGGTTTACCGCTACCTGGTAGGCT  >  minE/2209203‑2209330
                                                              |                                                                 
gCAGCTCAATGCCCACTTTACCATCCCACGGCACGCTGCCACTTAATGCTTCGTTCACCGCTGCAGGCa                                                             <  1:375781/69‑1 (MQ=255)
gCAGCTCAATGCCCACTTTACCATCCCACGCCACGCTGCCACTTAATGCTTCGTTCACCGCTGCAGGct                                                             <  1:1084653/69‑2 (MQ=255)
gCAGCTCAATGCCCACTTTACCATCCCACGCCACGCTGCCACTTAATGCTTCGTTCACCGCTGCAGGCa                                                             <  1:2008535/69‑1 (MQ=255)
gCAGCTCAATGCCCACTTTACCATCCCACGCCACGCTGCCACTTAATGCTTCGTTCACCGCTGCAGGCa                                                             <  1:3078578/69‑1 (MQ=255)
gCAGCTCAATGCCCACTTTACCATCCCACGCCACGCTGCCACTTAATGCTTCGTTCACCGCTGCAGGCa                                                             <  1:2598161/69‑1 (MQ=255)
gCAGCTCAATGCCCACTTTACCATCCCACGCCACGCTGCCACTTAATGCTTCGTTCACCGCTGCAGGCa                                                             <  1:2542226/69‑1 (MQ=255)
gCAGCTCAATGCCCACTTTACCATCCCACGCCACGCTGCCACTTAATGCTTCGTTCACCGCTGCAGGCa                                                             <  1:2447542/69‑1 (MQ=255)
gCAGCTCAATGCCCACTTTACCATCCCACGCCACGCTGCCACTTAATGCTTCGTTCACCGCTGCAGGCa                                                             <  1:3270562/69‑1 (MQ=255)
gCAGCTCAATGCCCACTTTACCATCCCACGCCACGCTGCCACTTAATGCTTCGTTCACCGCTGCAGGCa                                                             <  1:2311846/69‑1 (MQ=255)
gCAGCTCAATGCCCACTTTACCATCCCACGCCACGCTGCCACTTAATGCTTCGTTCACCGCTGCAGGCa                                                             <  1:2802799/69‑1 (MQ=255)
gCAGCTCAATGCCCACTTTACCATCCCACGCCACGCTGCCACTTAATGCTTCGTTCACCGCTGCAGGCa                                                             <  1:524175/69‑1 (MQ=255)
gCAGCTCAATGCCCACTTTACCATCCCACGCCACGCTGCCACTTAATGCTTCGTTCACCGCTGCAGGCa                                                             <  1:534064/69‑1 (MQ=255)
gCAGCTCAATGCCCACTTTACCATCCCACGCCACGCTGCCACTTAATGCTTCGTTCACCGCTGCAGGCa                                                             <  1:28359/69‑1 (MQ=255)
gCAGCTCAATGCCCACTTTACCATCCCACGCCACGCTGCCACTTAATGCTTCGTTCACCGCTGCAGGCa                                                             <  1:1892009/69‑1 (MQ=255)
gCAGCTCAATGCCCACTTTACCATCCCACGCCACGCTGCCACTTAATGCTTCGTTCACCGCTGCAGGCa                                                             <  1:1666195/69‑1 (MQ=255)
gCAGCTCAATGCCCACTTTACCATCCCACGCCACGCTGCCACTTAATGCTTCGTTCACCGCTGCAGGCa                                                             <  1:1599798/69‑1 (MQ=255)
gCAGCTCAATGCCCACTTTACCATCCCACGCCACGCTGCCACTTAATGCTTCGTTCACCGCTGCAGGCa                                                             <  1:2979703/69‑1 (MQ=255)
gCAGCTCAATGCCCACTTTACCATCCCACGCCACGCTGCCACTTAATGCTTCGTTCACCGCTGCAGGCa                                                             <  1:1075382/69‑1 (MQ=255)
gCAGCTCAATGCCCACTTTACCATCCCACGCCACGCTGCCACTTAATGCTTCGTTCACCGCTGCAGGCa                                                             <  1:1088711/69‑1 (MQ=255)
gCAGCTCAATGCCCACTTTACCATCCCACGCCACGCTGCCACTTAATGCTTCGTTCACCGCTGCAGGCa                                                             <  1:1178068/69‑1 (MQ=255)
gCAGCTCAATGCCCACTTTACCATCCCACGCCACGCTGCCACTTAATGCTTCGTTCACCGCTGCAGGCa                                                             <  1:1321568/69‑1 (MQ=255)
     tCAATGCCCACTTTACCATCCCACGCCACGCTGCCACTCAATGCTTCGTTCACCGCTTCAGGCAGAAc                                                         >  1:1513742/1‑68 (MQ=255)
     tCAATGCCCACTTTACCATCCCACGCCACGCTGCCACTCAATGCTTCGTTCACCGCTTCAGGCAGAACg                                                        >  1:2888967/1‑69 (MQ=255)
     tCAATGCCCACTTTACCATCCCACGCCACGCTGCCACTCAATGCTTCGTTCACCGCTTCAGGCAGAACg                                                        >  1:3236865/1‑69 (MQ=255)
           cccACTTTACCATCCCACGCCACGCTGCCACTCAATGCTTCGTTCACCGCTTCAGGCAGAACGcc                                                      <  1:2335615/65‑1 (MQ=255)
            ccACTTTACCATCCCACGCCACGCTGCCACTTAATGCTTCGTTCACCGCTGGAGGCAGAAAGCCGGttt                                                 >  1:100417/1‑69 (MQ=255)
            ccACTTTACCATCCCACGCCACGCTGCCACTTAATGCTTCGTTCACCGCTGCAGGCAGAAAGCCGGttt                                                 >  1:2401979/1‑69 (MQ=255)
            ccACTTTACCATCCCACGCCACGCTGCCACTTAATGCTTCGTTCACCGCTGCAGGCAGAAAGCCGGttt                                                 >  1:2971713/1‑69 (MQ=255)
            ccACTTTACCATCCCACGCCACGCTGCCACTTAATGCTTCGTTCACCGCTGCAGGCAGAAAGCCGGttt                                                 >  1:3125426/1‑69 (MQ=255)
            ccACTTTACCATCCCACGCCACGCTGCCACTTAATGCTTCGTTCACCGCTGCAGGCAGAAAGCCGGttt                                                 >  1:880731/1‑69 (MQ=255)
            ccACTTTACCATCCCACGCCACGCTGCCACTTAATGCTTCGTTCACCGCTGCAGGCAGAAAGCCGGttt                                                 >  1:1082859/1‑69 (MQ=255)
            ccACTTTACCATCCCACGCCACGCTGCCACTTAATGCTTCGTTCACCGCTGCAGGCAGAAAGCCGGttt                                                 >  1:868701/1‑69 (MQ=255)
            ccACTTTACCATCCCACGCCACGCTGCCACTTAATGCTTCGTTCACCGCTGCAGGCAGAAAGCCGGttt                                                 >  1:2493100/1‑69 (MQ=255)
            ccACTTTACCATCCCACGCCACGCTGCCACTTAATGCTTCGTTCACCGCTGCAGGCAGAAAGCCGGttt                                                 >  1:2878325/1‑69 (MQ=255)
            ccACTTTACCATCCCACGCCACGCTGCCACTTAATGCTTCGTTCACCGCTGCAGGCAGAAAGCCGGttt                                                 >  1:2750930/1‑69 (MQ=255)
            ccACTTTACCATCCCACGCCACGCTGCCACTTAATGCTTCGTTCACCGCTGCAGGCAGAAAGCCGGttt                                                 >  1:1458513/1‑69 (MQ=255)
            ccACTTTACCATCCCACGCCACGCTGCCACTTAATGCTTCGTTCACCGCTGCAGGCAGAAAGCCGGttt                                                 >  1:356371/1‑69 (MQ=255)
            ccACTTTACCATCCCACGCCACGCTGCCACTTAATGCTTCGTTCACCGCTGCAGGCAGAAAGCCGGttt                                                 >  1:1191090/1‑69 (MQ=255)
            ccACTTTACCATCCCACGCCACGCTGCCACTTAATGCTTCGTTCACCGCTGCAGGCAGAAAGCCGGttt                                                 >  1:458121/1‑69 (MQ=255)
            ccACTTTACCATCCCACGCCACGCTGCCACTTAATGCTTCGTTCACCGCTGCAGGCAGAAAGCCGGttt                                                 >  1:1284335/1‑69 (MQ=255)
            ccACTTTACCATCCCACGCCACGCTGCCACTTAATGCTTCGTTCACCGCTGCAGGCAGAAAGCCGGttt                                                 >  1:2804473/1‑69 (MQ=255)
            ccACTTTACCATCCCACGCCACGCTGCCACTTAATGCTTCGTTCACCGCTGCAGGCAGAAAGCCGGttt                                                 >  1:2042779/1‑69 (MQ=255)
            ccACTTTACCATCACACGCAACGCTGCCACTTAATGCTTCGTTCACCGCTGCAGGCAGAAAGCCGGttt                                                 >  1:3289621/1‑69 (MQ=255)
                  tACCATCCCACGCCACGCTGCCACTCAATGCTTCGTTCACCGCTTCAGGCAGAACGCCGGTTTTCGCCg                                           >  1:135517/1‑69 (MQ=255)
                  tACCATCCCACGCCACGCTGCCACTCAATGCTTCGTTCACCGCTTCAGGCAGAACGCCGGTTTTCGCCg                                           >  1:2176793/1‑69 (MQ=255)
                  tACCATCCCACGCCACGCTGCCACTCAATGCTTCGTTCACCGCTTCAGGCAGAACGCCGGTTTTCGCCg                                           >  1:1317547/1‑69 (MQ=255)
                  tACCATCCCACGCCACGCTGCCACTCAATGCTTCGTTCACCGCTTCAGGCAGAACGCCGGTTTTCGCCg                                           >  1:2856207/1‑69 (MQ=255)
                  tACCATCCCACGCCACGCTGCCACTCAATGCTTCGTTCACCGCTTCAGGCAGAACGCCGGTTTTCGCCg                                           >  1:265306/1‑69 (MQ=255)
                              ccacgcTGCCACTCAATGCTTCGTTCACCGCTTCAGGCAGAACGCCGGTTTTCGCCGGTTGCCAGTTAc                               <  1:2978804/69‑1 (MQ=255)
                                   cTGCCACTCAATGCTTCGTTCACCGCTTCAGGCAg                                                            >  1:2113925/1‑35 (MQ=255)
                                   cTGCCACTCAATGCTTCGTTCACCGCTTCAGGCAg                                                            >  1:776439/1‑35 (MQ=255)
                                   cTGCCACTCAATGCTTCGTTCACCGCTTCAGGCAg                                                            >  1:1885463/1‑35 (MQ=255)
                                   cTGCCACTCAATGCTTCGTTCACCGCTTCAGGCAg                                                            >  1:1664497/1‑35 (MQ=255)
                                   cTGCCACTCAATGCTTCGTTCACCGCTTCAGGCAg                                                            >  1:957393/1‑35 (MQ=255)
                                   cTGCCACTCAATGCTTCGTTCACCGCTTCAGGCAg                                                            >  1:1027064/1‑35 (MQ=255)
                                        aCTCAATGCTTCGTTCACCGCTTCAGGCAGAACGCCGGTTTTCGCCGGTTGCCAGTTACCGTTGAGGtt                     <  1:354775/69‑1 (MQ=255)
                                        aCTCAATGCTTCGTTCACCGCTTCAGGCAGAACGCCGGTTTTCGCCGGTTGCCAGTTACCGTTGAGGtt                     <  1:3021856/69‑1 (MQ=255)
                                        aCTCAATGCTTCGTTCACCGCTTCAGGCAGAACGCCGGTTTTCGCCGGTTGCCAGTTACCGTTGAGGtt                     <  1:2492850/69‑1 (MQ=255)
                                        aCTCAATGCTTCGTTCACCGCTTCAGGCAGAACGCCGGTTTTCGCCGGTTGCCAGTTACCGTTGAGGtt                     <  1:477970/69‑1 (MQ=255)
                                        aCTCAATGCTTCGTTCACCGCTTCAGGCAGAACGCCGGTTTTCGCCGGTTGCCAGTTACCGTTGAGGtt                     <  1:71090/69‑1 (MQ=255)
                                        aCTCAATGCTTCGTTCACCGCTTCAGGCAGAACGCCGGTTTTCGCCGGTTGCCAGTTACCGTTGAGGtt                     <  1:974101/69‑1 (MQ=255)
                                        aCTCAATGCTTCGTTCACCGCTTCAGGCAGAACGCCGGTTTTCGCCGGTTGCCAGTTACCGTTGAGGtt                     <  1:2502317/69‑1 (MQ=255)
                                        aCTCAATGCTTCGTTCACCGCTTCAGGCAGAACGCCGGTTTTCGCCGGTTGCCAGTTACCGTTGAGGtt                     <  1:2267036/69‑1 (MQ=255)
                                           cAATGCTTCGTTCACCGCTTCAGGCAGAACGCCGGTTTTCGCCGGTTGCCAGTTACCGTTGAGGTTTAc                  >  1:2678636/1‑69 (MQ=255)
                                              tGCTTCGTTCACCGCTTCAGGCAGAACGCCGGTTTTCGCCGGTTGCCAGTTACCGTTGAGGTTTACCGc               <  1:1544917/69‑1 (MQ=255)
                                                cTTCGTTCACCGCTTCAGGCAGAACGCCGGTTTTCGCCGGTTGCCAGt                                  >  1:2760314/1‑48 (MQ=255)
                                                cTTCGTTCACCGCTTCAGGCAGAACGCCGGTTTTCGCCGGTTGCCAGTTACCGTTGAGGTTTACCGCTa             >  1:1999363/1‑69 (MQ=255)
                                                cTTCGTTCACCGCTTCAGGCAGAACGCCGGTTTTCGCCGGTTGCCAGTTACCGTTGAGGTTTACCGCTa             >  1:49687/1‑69 (MQ=255)
                                                cTTCGTTCACCGCTTCAGGCAGAACGCCGGTTTTCGCCGGTTGCCAGTTACCGTTGAGGTTTACCGCTa             >  1:1172666/1‑69 (MQ=255)
                                                cTTCGTTCACCGCTTCAGGCAGAACGCCGGTTTTCGCCGGTTGCCAGTTACCGTTGAGGTTTACCGCTa             >  1:2408530/1‑69 (MQ=255)
                                                cTTCGTTCACCGCTTCAGGCAGAACGCCGGTTTTCGCCGGTTGCCAGTTACCGTTGAGGTTTACCGCTa             >  1:3387960/1‑69 (MQ=255)
                                                cTTCGTTCACCGCTTCAGGCAGAACGCCGGTTTTCGCCGGTTGCCAGTTACCGTTGAGGTTTACCGCTa             >  1:2435416/1‑69 (MQ=255)
                                                cTTCGTTCACCGCTTCAGGCAGAACGCCGGTTTTCGCCGGTTGCCAGTTACCGTTGAGGTTTACCGCTa             >  1:2439300/1‑69 (MQ=255)
                                                cTTCGTTCACCGCTTCAGGCAGAACGCCGGTTTTCGCCGGTTGCCAGTTACCGTTGAGGTTTACCGCTa             >  1:2745215/1‑69 (MQ=255)
                                                           gCTTCAGGCAGAACGCCTGTTTTCGCCGGTTGCCAGTTACCGTTGAGGTTTACCGCTACCTGGTAGGCt  <  1:210117/69‑1 (MQ=255)
                                                           gCTTCAGGCAGAACGCCGGTTTTCGCCGGTTGCCAGTTACCGTTGAGGTTTACCGCTACCTGGTAGGCt  <  1:977261/69‑1 (MQ=255)
                                                           gCTTCAGGCAGAACGCCGGTTTTCGCCGGTTGCCAGTTACCGTTGAGGTTTACCGCTACCTGGTAGGCt  <  1:18141/69‑1 (MQ=255)
                                                           gCTTCAGGCAGAACGCCGGTTTTCGCCGGTTGCCAGTTACCGTTGAGGTTTACCGCTACCTGGTAGGCt  <  1:1852623/69‑1 (MQ=255)
                                                           gCTTCAGGCAGAACGCCGGTTTTCGCCGGTTGCCAGTTACCGTTGAGGTTTACCGCTACCTGGTAGGCt  <  1:3064940/69‑1 (MQ=255)
                                                           gCTTCAGGCAGAACGCCGGTTTTCGCCGGTTGCCAGTTACCGTTGAGGTTTACCGCTACCTGGTAGGCt  <  1:1908385/69‑1 (MQ=255)
                                                           gCTTCAGGCAGAACGCCGGTTTTCGCCGGTTGCCAGTTACCGTTGAGGTTTACCGCTACCTGGTAGGCt  <  1:2049527/69‑1 (MQ=255)
                                                           gCTTCAGGCAGAACGCCGGTTTTCGCCGGTTGCCAGTTACCGTTGAGGTTTACCGCTACCTGGTAGGCt  <  1:2066221/69‑1 (MQ=255)
                                                           gCTTCAGGCAGAACGCCGGTTTTCGCCGGTTGCCAGTTACCGTTGAGGTTTACCGCTACCTGGTAGGCt  <  1:2156761/69‑1 (MQ=255)
                                                           gCTTCAGGCAGAACGCCGGTTTTCGCCGGTTGCCAGTTACCGTTGAGGTTTACCGCTACCTGGTAGGCt  <  1:3334591/69‑1 (MQ=255)
                                                            cTTCAGGCAGAACGCCGGTTTTCGCCGGTTGCCAGTTACCGTTGAGGTTTACCGCTACCTGGTAGGCt  <  1:2131870/68‑1 (MQ=255)
                                                              |                                                                 
GCAGATCAATGCCCACTTTACCATCCCACGCCACGCTGCCACTCAATGCTTCGTTCACCGCTTCAGGCAGAACGCCGGTTTTCGCCGGTTGCCAGTTACCGTTGAGGTTTACCGCTACCTGGTAGGCT  >  minE/2209203‑2209330

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: