| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,209,600 | A→G | 34.7% | S647S (TCT→TCC) | yhdP ← | conserved membrane protein, predicted transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,209,600 | 0 | A | G | 34.7% | 63.4 / 53.0 | 92 | S647S (TCT→TCC) | yhdP | conserved membrane protein, predicted transporter |
| Reads supporting (aligned to +/- strand): ref base A (49/11); new base G (15/17); total (64/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.48e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.37e-01 | |||||||||||
AAGATGTAAGCGAGCATTCACATCGCCGTCGAGCTGGAGTTCTTGCAGGGTCGCACCCAGAGAATCTTTCAGCGGTGTCTCATCAAAGTAAGGGCCAACGGCTTTA‑CCCGGACCTTTA > minE/2209540‑2209657 | aaGATGTAAGCGAGCATTCACATCGCCGTCGAGCTGGAGTTCTTGCAGGGTCGCACCCAGAGAATCt > 1:995020/1‑67 (MQ=255)aaGATGTAAGCGAGCATTCACATCGCCGTCGAGCTGGAGTTCTTGCAGGGTCGCACCCAGAGAATCt > 1:965260/1‑67 (MQ=255)aaGATGTAAGCGAGCATTCACATCGCCGTCGAGCTGGAGTTCTTGCAGGGTCGCACCCAGAGAATCt > 1:2263684/1‑67 (MQ=255)aaGATGTAAGCGAGCATTCACATCGCCGTCGAGCTGGAGTTCTTGCAGGGTCGCACCCAGAGAATCt > 1:2908453/1‑67 (MQ=255)aaGATGTAAGCGAGCATTCACATCGCCGTCGAGCTGGAGTTCTTGCAGGGTCGCACCCAGAGAATCt > 1:844897/1‑67 (MQ=255)aaGATGTAAGCGAGCATTCACATCGCCGTCGAGCTGGAGTTCTTGCAGGGTCGCACCCAGAGAATCt > 1:2940310/1‑67 (MQ=255)aaGATGTAAGCGAGCATTCACATCGCCGTCGAGCTGGAGTTCTTGCAGGGTCGCACCCAGAGAATCt > 1:736701/1‑67 (MQ=255)aaGATGTAAGCGAGCATTCACATCGCCGTCGAGCTGGAGTTCTTGCAGGGTCGCACCCAGAGAATCt > 1:3375747/1‑67 (MQ=255)aaGATGTAAGCGAGCATTCACATCGCCGTCGAGCTGGAGTTCTTGCAGGGTCGCACCCAGAGAATCt > 1:3409161/1‑67 (MQ=255)aaGATGTAAGCGAGCATTCACATCGCCGTCGAGCTGGAGTTCTTGCAGGGTCGCACCCAGAGAATCt > 1:65444/1‑67 (MQ=255)aaGATGTAAGCGAGCATTCACATCGCCGTCGAGCTGGAGTTCTTGCAGGGTCGCACCCAGAGAATCt > 1:1618512/1‑67 (MQ=255) gTAAGCGAGCATTCACATCGCCGTCGAGCTGGAGTTCTTGCAGGGTCGCACCCAGAGAATCTTTCAGCg > 1:1906788/1‑69 (MQ=255) gTAAGCGAGCATTCACATCGCCGTCGAGCTGGAGTTCTTGCAGGGTCGCACCCAGAGAATCTTTCAGCg > 1:3065620/1‑69 (MQ=255) gTAAGCGAGCATTCACATCGCCGTCGAGCTGGAGTTCTTGCAGGGTCGCACCCAGAGAATCTTTCAGCg > 1:1519482/1‑69 (MQ=255) gTAAGCGAGCATTCACATCGCCGTCGAGCTGGAGTTCTTGCAGGGTCGCACCCAGAGAATCTTTCAGCg > 1:718031/1‑69 (MQ=255) gTAAGCGAGCATTCACATCGCCGTCGAGCTGGAGTTCTTGCAGGGTCGCACCCAGAGAATCTTTCAGCg > 1:1312114/1‑69 (MQ=255) gTAAGCGAGCATTCACATCGCCGTCGAGCTGGAGTTCTTGCAGGGTCGCACCCAGAGAATCTTTCAGCg > 1:929103/1‑69 (MQ=255) tAAGCGAGCATTCACATCGCCGTCGAGCTGGAGTTCTTGCAGGGTCGCACCCAGAGAATCTTTCAGCgg > 1:54982/1‑69 (MQ=255) tAAGCGAGCATTCACATCGCCGTCGAGCTGGAGTTCTTGCAGGGTCGCACCCAGAGAATCTTTCAGCgg > 1:415899/1‑69 (MQ=255) tAAGCGAGCATTCACATCGCCGTCGAGCTGGAGTTCTTGCAGGGTCGCACCCAGAGAATCTTTCAGCgg > 1:396872/1‑69 (MQ=255) tAAGCGAGCATTCACATCGCCGTCGAGCTGGAGTTCTTGCAGGGTCGCACCCAGAGAATCTTTCAGCgg > 1:658317/1‑69 (MQ=255) tAAGCGAGCATTCACATCGCCGTCGAGCTGGAGTTCTTGCAGGGTCGCACCCAGAGAATCTTTCAGCgg > 1:745877/1‑69 (MQ=255) tAAGCGAGCATTCACATCGCCGTCGAGCTGGAGTTCTTGCAGGGTCGCACCCAGAGAATCTTTCAGCgg > 1:2857591/1‑69 (MQ=255) tAAGCGAGCATTCACATCGCCGTCGAGCTGGAGTTCTTGCAGGGTCGCACCCAGAGAATCTTTCAGCgg > 1:1970635/1‑69 (MQ=255) tAAGCGAGCATTCACATCGCCGTCGAGCTGGAGTTCTTGCAGGGTCGCACCCAGAGAATCTTTCAGCgg > 1:2879436/1‑69 (MQ=255) tAAGCGAGCATTCACATCGCCGTCGAGCTGGAGTTCTTGCAGGGTCGCACCCAGAGAATCTTTCAGCgg > 1:1734616/1‑69 (MQ=255) tAAGCGAGCATTCACATCGCCGTCGAGCTGGAGTTCTTGCAGGGTCGCACCCAGAGAATCTTTCAGCgg > 1:1598710/1‑69 (MQ=255) tAAGCGAGCATTCACATCGCCGTCGAGCTGGAGTTCTTGCAGGGTCGCACCCAGAGAATCTTTCAGCgg > 1:1899941/1‑69 (MQ=255) tAAGCGAGCATTCACATCGCCGTCGAGCTGGAGTTCTTGCAGGGTCGCACCCAGAGAATCTTTCAGCg > 1:2288053/1‑68 (MQ=255) tAAGCGAGCATTCACATCGCCGTCGAGCTGGAGTTCTTGCAGGGTCGCACCCAGAGAATCTTTCAGCg > 1:2859185/1‑68 (MQ=255) tAAGCGAGCATTCACATCGCCGTCGAGCTGGAGTTCTTGCAGGGTCGCACCCAGAGAATCTTTCAGCg > 1:87206/1‑68 (MQ=255) tAAGCGAGCATTCACATCGCCGTCGAGCTGGAGTTCTTGCAGGGTCGCACCCAGAGAATCTTTCAGCg > 1:2032962/1‑68 (MQ=255) tAAGCGAGCATTCACATCGCCGTAGAGCTGGAGTTCTTGCAGGGTCGCACCCAGAGAATCTTTCAGCgg > 1:2520529/1‑69 (MQ=255) tAAGCGAGCATTCACATCGCCGTAGAGCTGGAGTTCTTGCAGGGTCGCACCCAGAGAATCTTTCAGCg > 1:2719508/1‑68 (MQ=255) cATTCACATCGCCGTCGAGCTGGAGTTCTTGCAGGGTCGCACCCAGGTAATCTTTCAGCGGTGTCtca > 1:2297375/1‑68 (MQ=255) cATTCACATCGCCGTCGAGCTGGAGTTCTTGCAGGGTCGCACCCAGGGAATCTTTCAGCGGTGtctc > 1:609100/1‑67 (MQ=255) cATTCACATCGCCGTCGAGCTGGAGTTCTTGCAGGGTCGCACCCAGGGAATCTTTCAGCGGTGTCtca > 1:1669088/1‑68 (MQ=255) cATTCACATCGCCGTCGAGCTGGAGTTCTTGCAGGGTCGCACCCAGGGAATCTTTCAGCGGTGTCtca > 1:1321503/1‑68 (MQ=255) cATTCACATCGCCGTCGAGCTGGAGTTCTTGCAGGGTCGCACCCAGGGAATCTTTCAGCGGTGTCtca > 1:3324567/1‑68 (MQ=255) cATTCACATCGCCGTCGAGCTGGAGTTCTTGCAGGGTCGCACCCAGGGAATCTTTCAGCGGTGTCtca > 1:1154617/1‑68 (MQ=255) cATTCACATCGCCGTCGAGCTGGAGTTCTTGCAGGGTCGCACCCAGGGAATCTTTCAGCGGTGTCtca > 1:1080916/1‑68 (MQ=255) cATTCACATCGCCGTCGAGCTGGAGTTCTTGCAGGGTCGCACCCAGGGAATCTTTCAGCGGTGTCtca > 1:3044427/1‑68 (MQ=255) cATTCACATCGCCGTCGAGCTGGAGTTCTTGCAGGGTCGCACCCAGGGAATCTTTCAGCGGTGTCtca > 1:1837230/1‑68 (MQ=255) cATTCACATCGCCGTCGAGCTGGAGTTCTTGCAGGGTCGCACCCAGGGAATCTTTCAGCGGTGTCtca > 1:467839/1‑68 (MQ=255) cATTCACATCGCCGTCGAGCTGGAGTTCTTGCAGGGTCGCACCCAGGGAATCTTTCAGCGGTGTCtca > 1:2522404/1‑68 (MQ=255) cATTCACATCGCCGTCGAGCTGGAGTTCTTGCAGGGTCGCACCCAGGGAATCTTTCAGCGGTGTCtca > 1:1879541/1‑68 (MQ=255) cATTCACATCGCCGTCGAGCTGGAGTTCTTGCAGGGTCGCACCCAGGGAATCTTTCAGCGGTGTCtca > 1:613055/1‑68 (MQ=255) cATTCACATCGCCGTCGAGCTGGAGTTCTTGCAGGGTCGCACCCAGGGAATCTTTCAGCGGTGTCtca > 1:1485411/1‑68 (MQ=255) cATTCACATCGCCGTCGAGCTGGAGTTCTTGCAGGGTCGCACCCAGGGAATATTTCAGCGGTGTCtca > 1:2160339/1‑68 (MQ=255) cacaTCGCCGTCGAGCTGGAGTTCTTGCAGGGTCGCACCCAGACAATCTTTCAGCgg > 1:210251/1‑57 (MQ=255) aTCGCCGTCGAGCTGGAGTTCTTGCAGGGTCGCACCCAGGGAATCTTTCAGCGGTGTCTCATCAAAGTa < 1:435857/69‑1 (MQ=255) aTCGCCGTCGAGCTGGAGTTCTTGCAGGGTCGCACCCAGGGAATCTTTCAGCGGTGTCTCATCAAAGTa < 1:1884562/69‑1 (MQ=255) aTCGCCGTCGAGCTGGAGTTCTTGCAGGGTCGCACCCAGGGAATCTTTCAGCGGTGTCTCATCAAAGTa < 1:354381/69‑1 (MQ=255) aTCGCCGTCGAGCTGGAGTTCTTGCAGGGTCGCACCCAGGGAATCTTTCAGCGGTGTCTCATCAAAGTa < 1:1711751/69‑1 (MQ=255) aTCGCCGTCGAGCTGGAGTTCTTGCAGGGTCGCACCCAGGGAATCTTTCAGCGGTGTCTCATCAAAGTa < 1:3250606/69‑1 (MQ=255) aTCGCCGTCGAGCTGGAGTTCTTGCAGGGTCGCACCCAGGGAATCTTTCAGCGGTGTCTCATCAAAGTa < 1:1552570/69‑1 (MQ=255) aTCGCCGTCGAGCTGGAGTTCTTGCAGGGTCGCACCCAGGGAATCTTTCAGCGGTGTCTCATCAAAGTa < 1:303965/69‑1 (MQ=255) aTCGCCGTCGAGCTGGAGTTCTTGCAGGGTCGCACCCAGGGAATCTTTCAGCGGTGTCTCATCAAAGTa < 1:2145513/69‑1 (MQ=255) aTCGCCGTCGAGCTGGAGTTCTTGCAGGGTCGCACCCAGGGAATCTTTCAGCGGTGTCTCATCAAAGTa < 1:2371451/69‑1 (MQ=255) aTCGCCGTCGAGCTGGAGTTCTTGCAGGGTCGCACCCAGGGAATCTTTCAGCGGTGTCTCATCAAAGTa < 1:2857048/69‑1 (MQ=255) aTCGCCGTCGAGCTGGAGTTCTTGCAGGGTCGCACCCAGGGAATCTTTCAGCGGTGTCTCATCAAAGTa < 1:2895239/69‑1 (MQ=255) aTCGCCGTCGAGCTGGAGTTCTTGCAGGGTCGCACCCAGGGAATCTTTCAGCGGTGTCTCATCAAAGTa < 1:2899611/69‑1 (MQ=255) aTCGCCGTCGAGCTGGAGTTCTTGCAGGGTCGCACCCAGGGAATCTTTCAGCGGTGTCTCATCAAAGTa < 1:1812200/69‑1 (MQ=255) aTCGCCGTCGAGCTGGAGTTCTTGCAGGGTCGCACCCAGGGAATCTTTCAGCGGTGTCTCATCAAAGTa < 1:1751144/69‑1 (MQ=255) aTCGCCGTCGAGCTGGAGTTCTTGCAGGGTCGCACCCAGGGAATCTTTCAGCGGTGTCTCATCAAAGTa < 1:868993/69‑1 (MQ=255) aTCGCCGTCGAGCTGGAGTTCTTGCAGGGTCGCACCCAGAGAATCTTTCAGCGGTGTCTCATCAAAGt < 1:590010/68‑1 (MQ=255) tCGCCGTCGAGCTGGAGTTCTTGCAGGGTCGCACCCAGGGAATCTTTCAGCGGTGTCTCATCAAAGTa < 1:1671725/68‑1 (MQ=255) gCCGTCGAGCTGGAGTTCTTGCAGGGTCGCACCCAGAGAATCTTTCAGCGGTGTCtcatc > 1:839247/1‑60 (MQ=255) gCCGTCGAGCTGGAGTTCTTGCAGGGTCGCACCCAGAGAATCTTTCAGCGGTGTCTCATCAAAGTAAgg > 1:1630893/1‑69 (MQ=255) gCCGTCGAGCTGGAGTTCTTGCAGGGTCGCACCCAGAGAATCTTTCAGCGGTGTCTCATCAAAGTAAgg > 1:895547/1‑69 (MQ=255) gCCGTCGAGCTGGAGTTCTTGCAGGGTCGCACCCAGAGAATCTTTCAGCGGTGTCTCATCAAAGTAAgg > 1:477449/1‑69 (MQ=255) gCCGTCGAGCTGGAGTTCTTGCAGGGTCGCACCCAGAGAATCTTTCAGCGGTGTCTCATCAAAGTAAgg > 1:3147899/1‑69 (MQ=255) gCCGTCGAGCTGGAGTTCTTGCAGGGTCGCACCCAGAGAATCTTTCAGCGGTGTCTCATCAAAGTAAgg > 1:2042199/1‑69 (MQ=255) cGTCGAGCTGGAGTTCTTGCAGGGTCGCACCCAGAGAATCTTTCAGCGGTGTCTCATCAAAGTAAggg > 1:980060/1‑68 (MQ=255) cGTCGAGCTGGAGTTCTTGCAGGGTCGCACCCAGAGAATCTTTCAGCGGTGTCTCATCAAAGTAAggg > 1:86751/1‑68 (MQ=255) cGTCGAGCTGGAGTTCTTGCAGGGTCGCACCCAGAGAATCTTTCAGCGGTGTCTCATCAAAGTAAggg > 1:1132542/1‑68 (MQ=255) cGTCGAGCTGGAGTTCTTGCAGGGTCGCACCCAGAGAATCTTTCAGCGGTGTCTCATCAAAGTAAggg > 1:1384536/1‑68 (MQ=255) cGTCGAGCTGGAGTTCTTGCAGGGTCGCACCCAGAGAATCTTTCAGCGGTGTCTCATCAAAGTAAggg > 1:294243/1‑68 (MQ=255) cGTCGAGCTGGAGTTCTTGCAGGGTCGCACCCAGAGAATCTTTCAGCGGTGTCTCATCAAAGTAAggg > 1:2806187/1‑68 (MQ=255) cGTCGAGCTGGAGTTCTTGCAGGGTCGCACCCAGAGAATCTTTCAGCGGTGTCTCATCAAAGTAAggg > 1:2523351/1‑68 (MQ=255) cTGGAGTTCTTGCAGGGTCGCACCCAGAGAATCTTTCAGCGGTGTCTCATCAAAGTAAGGGCCAACGGc < 1:3429333/69‑1 (MQ=255) tgAGTTCTTGCAGGGTCGCACCCAGGGTATCTTTCAGCGGTGTCTCATCAAAGTa < 1:1467668/54‑1 (MQ=255) ggTCGCACCCAGAGAATCTTTCAGCGGTGTCTCATCAAAGTAAGGGCCAACGGCTTTA‑CCCGGACCttt < 1:1357034/69‑1 (MQ=255) ggTCGCACCCAGAGAATCTTTCAGCGGTGTCTCATCAAAGTAAGGGCCAACGGCTTTA‑CCCGGACCttt < 1:3305669/69‑1 (MQ=255) ggTCGCACCCAGAGAATCTTTCAGCGGTGTCTCATCAAAGTAAGGGCCAACGGCTTTA‑CCCGGACCttt < 1:2880501/69‑1 (MQ=255) ggTCGCACCCAGAGAATCTTTCAGCGGTGTCTCATCAAAGTAAGGGCCAACGGCTTTA‑CCCGGACCttt < 1:911917/69‑1 (MQ=255) ggTCGCACCCAGAGAATCTTTCAGCGGTGTCTCATCAAAGTAAGGGCCAACGGCTTTA‑CCCGGACCttt < 1:981523/69‑1 (MQ=255) gTCGCACCCAGAGAATCTTTCAGCGGTGTCTCATCAAAGTAAGGGCCAACGGCTTTA‑CCCGGACCttt < 1:921240/68‑1 (MQ=255) gTCGCACCCAGAGAATCTTTCAGCGGTGTCTCATCAAAGTAAGGGCCAACGGCTTTA‑CCCGGACCTTTa < 1:823336/69‑1 (MQ=255) gTCGCACCCAGAGAATCTTTCAGCGGTGTCTCATCAAAGTAAGGGCCAACGGCTTTA‑CCCGGACCTTTa < 1:2671187/69‑1 (MQ=255) gTCGCACCCAGAGAATCTTTCAGCGGTGGCTCATCAAAGTAAGGGCCAACGGCTTTA‑CCCGGACCTTTa < 1:1217012/69‑1 (MQ=255) tCGCACCCAGAGAATCTTTCAGCGGTGTCTCATCAAAGTAAGGGCCAACGGCTTTACCCCGGACCTTTa > 1:216536/1‑69 (MQ=255) | AAGATGTAAGCGAGCATTCACATCGCCGTCGAGCTGGAGTTCTTGCAGGGTCGCACCCAGAGAATCTTTCAGCGGTGTCTCATCAAAGTAAGGGCCAACGGCTTTA‑CCCGGACCTTTA > minE/2209540‑2209657 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |