| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,210,179 | A→C | 29.6% | T454T (ACT→ACG) | yhdP ← | conserved membrane protein, predicted transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,210,179 | 0 | A | C | 29.6% | 104.3 / 66.0 | 98 | T454T (ACT→ACG) | yhdP | conserved membrane protein, predicted transporter |
| Reads supporting (aligned to +/- strand): ref base A (17/52); new base C (17/12); total (34/64) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.21e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01 | |||||||||||
TTGGCTTTAACGTCAATATTACGCCCATCCAGCTGGAAACCTTTGTTATTGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGGCGCACGGAATACCGTTTCGTAAGGCATCTTTG > minE/2210114‑2210240 | ttggctttAACGTCAATATTACGCCCATCCAGCTGGAAACCTTTGTTATTGTTCAGCCAGCTTATAGtt > 1:1146457/1‑69 (MQ=255) ttAACGTCAATATTACGCCCATCCAGCTGGAAACCTTTGTCATTGTTCAGCCAGCTTATCGTTGCCTgg > 1:286350/1‑69 (MQ=255) ttAACGTCAATATTACGCCCATCCAGCTGGAAACCTTTGTCATTGTTCAGCCAGCTTATCGTTGCCTgg > 1:2185524/1‑69 (MQ=255) ttAACGTCAATATTACGCCCATCCAGCTGGAAACCTTTGTCATTGTTCAGCCAGCTTATCGTTGCCTgg > 1:221106/1‑69 (MQ=255) ttAACGTCAATATTACGCCCATCCAGCTGGAAACCTTTGTCATTGTTCAGCCAGCTTATCGTTGCCTgg > 1:2225753/1‑69 (MQ=255) ttAACGTCAATATTACGCCCATCCAGCTGGAAACCTTTGTCATTGTTCAGCCAGCTTATCGTTGCCTgg > 1:1534108/1‑69 (MQ=255) ttAACGTCAATATTACGCCCATCCAGCTGGAAACCTTTGTCATTGTTCAGCCAGCTTATCGTTGCCTgg > 1:2311982/1‑69 (MQ=255) ttAACGTCAATATTACGCCCATCCAGCTGGAAACCTTTGTCATTGTTCAGCCAGCTTATCGTTGCCTgg > 1:2602716/1‑69 (MQ=255) ttAACGTCAATATTACGCCCATCCAGCTGGAAACCTTTGTCATTGTTCAGCCAGCTTATCGTTGCCTgg > 1:948622/1‑69 (MQ=255) ttAACGTCAATATTACGCCCATCCAGCTGGAAACCTTTGTCATTGTTCAGCCAGCTTATCGTTGCCTgg > 1:2673968/1‑69 (MQ=255) ttAACGTCAATATTACGCCCATCCAGCTGGAAACCTTTGTCATTGTTCAGCCAGCTTATCGTTGCCTgg > 1:2921376/1‑69 (MQ=255) ttAACGTCAATATTACGCCCATCCAGCTGGAAACCTTTGTCATTGTTCAGCCAGCTTATCGTTGCCTgg > 1:128381/1‑69 (MQ=255) ttAACGTCAATATTACGCCCATCCAGCTGGAAACCTTTGTCATTGTTCAGCCAGCTTATCGTTGCCTgg > 1:3242212/1‑69 (MQ=255) ttAACGTCAATATTACGCCCATCCAGCTGGAAACCTTTGTCATTGTTCAGCCAGCTTATCGTTGCCTgg > 1:3328134/1‑69 (MQ=255) ttAACGTCAATATTACGCCCATCCAGCTGGAAACCTTTGTCATTGTTCAGCCAGCTTATCGTTGCCTgg > 1:419175/1‑69 (MQ=255) ttAACGTCAATATTACGCCCATCCAGCTGGAAACCTTTGTCATTGTTCAGCCAGCTTATCGTTGCCTgg > 1:466640/1‑69 (MQ=255) ttAACGTCAATATTACGCCCATCCAGCTGGAAACCTTTGTCATTGTTCAGCCAGCTTATCGTTGCCTgg > 1:547395/1‑69 (MQ=255) ttAACGTCAATATTACGCCCATCCAGCTGGAAACCTTTGTCATTGTTCAGCCAGCTTATCGTTGCCTg > 1:789975/1‑68 (MQ=255) gTCAATATTACGCCCATCCAGCTGGAAACCTTTGTTATTGTTCAGCCAGCTTATAGTTGCCTGGCCGt > 1:1739727/1‑68 (MQ=255) gTCAATATTACGCCCATCCAGCTGGAAACCTTTGTTATTGTTCAGCCAGCTTATAGTTGCCTGGCCGTc > 1:2810146/1‑69 (MQ=255) ccAGCTGGAAACCTTTGTTATTGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGgcg < 1:2318712/69‑1 (MQ=255) ccAGCTGGAAACCTTTGTTATTGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGgcg < 1:2823238/69‑1 (MQ=255) ccAGCTGGAAACCTTTGTTATTGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGgcg < 1:1278967/69‑1 (MQ=255) ccAGCTGGAAACCTTTGTTATTGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGgcg < 1:483914/69‑1 (MQ=255) ccAGCTGGAAACCTTTGTTATTGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGgcg < 1:3019891/69‑1 (MQ=255) ccAGCTGGAAACCTTTGTTATTGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGgcg < 1:3023963/69‑1 (MQ=255) ccAGCTGGAAACCTTTGTTATTGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGgcg < 1:3196593/69‑1 (MQ=255) ccAGCTGGAAACCTTTGTTATTGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGgcg < 1:1862030/69‑1 (MQ=255) cAGCTGGAAACCTTTGTTATTGTTCGGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGgcgc > 1:2371171/1‑69 (MQ=255) cAGCTGGAAACCTTTGTTATTGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGgcgc > 1:51627/1‑69 (MQ=255) cAGCTGGAAACCTTTGTTATTGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGgcgc > 1:2063979/1‑69 (MQ=255) cAGCTGGAAACCTTTGTTATTGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGgcgc > 1:2983256/1‑69 (MQ=255) cAGCTGGAAACCTTTGTTATTGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGgcgc > 1:3223960/1‑69 (MQ=255) cAGCTGGAAACCTTTGTTATTGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGgcgc > 1:955685/1‑69 (MQ=255) cAGCTGGAAACCTTTGTTATTGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGgcgc > 1:756263/1‑69 (MQ=255) cAGCTGGAAACCTTTGTTATTGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGgcg > 1:2524827/1‑68 (MQ=255) cAGCTGGAAACCTTTGTTATTGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGgcg > 1:1474400/1‑68 (MQ=255) tgtaaaCCTTTGTTATTGTTCATCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGGCGCACgg < 1:1256439/66‑1 (MQ=255) tGGAAACCTTTGTTATTGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGGCGCACgg < 1:233546/69‑1 (MQ=255) tGGAAACCTTTGTTATTGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGGCGCACgg < 1:518240/69‑1 (MQ=255) tGGAAACCTTTGTTATTGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGGCGCACgg < 1:3083236/69‑1 (MQ=255) tGGAAACCTTTGTTATTGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGGCGCACgg < 1:3077790/69‑1 (MQ=255) tGGAAACCTTTGTTATTGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGGCGCACgg < 1:2001769/69‑1 (MQ=255) tGGAAACCTTTGTTATTGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGGCGCACgg < 1:698585/69‑1 (MQ=255) tGGAAACCTTTGTTATTGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGGCGCACgg < 1:1585351/69‑1 (MQ=255) tGGAAACCTTTGTTATTGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGGCGCACgg < 1:1384828/69‑1 (MQ=255) tGGAAACCTTTGTTATTGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGGCGCACgg < 1:2890540/69‑1 (MQ=255) tGGAAACCTTTGTTATTGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGGCGCACgg < 1:2868600/69‑1 (MQ=255) tGGAAACCTTTGTTATTGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGGCGCACgg < 1:3242234/69‑1 (MQ=255) tGGAAACCTTTGTTATTGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGGCGCACgg < 1:583971/69‑1 (MQ=255) tGGAAACCTTTGTTATTGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGGCGCACgg < 1:2697496/69‑1 (MQ=255) tGGAAACCTTTGTTATTGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGGCGCACgg < 1:2319218/69‑1 (MQ=255) tGGAAACCTTTGTTATTGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGGCGCACgg < 1:1234955/69‑1 (MQ=255) tGGAAACCTTTGTTATTGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGGCGCACgg < 1:3331700/69‑1 (MQ=255) tGGAAACCTTTGTTATTGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGGCGCACgg < 1:2643743/69‑1 (MQ=255) tGGAAACCTTTGTTATTGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGGCGCACgg < 1:2498209/69‑1 (MQ=255) tGGAAACCTTTGTTATTGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGGCGCACgg < 1:589373/69‑1 (MQ=255) tGGAAACCTTTGTTATTGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGGCGCACgg < 1:2580609/69‑1 (MQ=255) ttGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTGTa > 1:1446326/1‑43 (MQ=255) ttGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTa > 1:2141374/1‑43 (MQ=255) ttGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTa > 1:1964678/1‑43 (MQ=255) ttGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTa > 1:324693/1‑43 (MQ=255) ttGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTa > 1:3149643/1‑43 (MQ=255) ttGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGGCGCACgg < 1:1581525/54‑1 (MQ=255) tGTTCAGCCAGCTTATCGTTGCCTGGCCGTCGGCGATTTCCAGTGGCGCACGGAATACCGTTTCGTAAg < 1:1010402/69‑1 (MQ=255) tGTTCAGCCAGCTTATCGTTGCCTGGCCGTCGGCGATTTCCAGTGGCGCACGGAATACCGTTTCGTAAg < 1:427261/69‑1 (MQ=255) tGTTCAGCCAGCTTATCGTTGCCTGGCCGTCGGCGATTTCCAGTGGCGCACGGAATACCGTTTCGTAAg < 1:463544/69‑1 (MQ=255) tGTTCAGCCAGCTTATCGTTGCCTGGCCGTCGGCGATTTCCAGTGGCGCACGGAATACCGTTTCGTAAg < 1:310325/69‑1 (MQ=255) tGTTCAGCCAGCTTATCGTTGCCTGGCCGTCGGCGATTTCCAGTGGCGCACGGAATACCGTTTCGTAAg < 1:1918642/69‑1 (MQ=255) tGTTCAGCCAGCTTATCGTTGCCTGGCCGTCGGCGATTTCCAGTGGCGCACGGAATACCGTTTCGTAAg < 1:2109142/69‑1 (MQ=255) tGTTCAGCCAGCTTATCGTTGCCTGGCCGTCGGCGATTTCCAGTGGCGCACGGAATACCGTTTCGTAAg < 1:2260013/69‑1 (MQ=255) tGTTCAGCCAGCTTATCGTTGCCTGGCCGTCGGCGATTTCCAGTGGCGCACGGAATACCGTTTCGTAAg < 1:802641/69‑1 (MQ=255) tGTTCAGCCAGCTTATCGTTGCCTGGCCGTCGGCGATTTCCAGTGGCGCACGGAATACCGTTTCGTAAg < 1:2306608/69‑1 (MQ=255) tGTTCAGCCAGCTTATCGTTGCCTGGCCGTCGGCGATTTCCAGTGGCGCACGGAATACCGTTTCGTAAg < 1:1473693/69‑1 (MQ=255) tGTTCAGCCAGCTTATCGTTGCCTGGCCGTCGGCGATTTCCAGTGGCGCACGGAATACCGTTTCGTAAg < 1:1391871/69‑1 (MQ=255) tGTTCAGCCAGCTTATCGTTGCCTGGCCGTCGGCGATTTCCAGTGGCGCACGGAATACCGTTTCGTAAg < 1:2905816/69‑1 (MQ=255) gTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGa < 1:2438228/35‑1 (MQ=255) gTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGa < 1:2301125/35‑1 (MQ=255) gTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGa < 1:3060812/35‑1 (MQ=255) gTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGa < 1:1951873/35‑1 (MQ=255) gTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGa < 1:3131600/35‑1 (MQ=255) cagccagcTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGGCGCACGGAATACCGTTTCGTAAGGCAt < 1:3309435/69‑1 (MQ=255) cagccagcTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGGCGCACGGAATACCGTTTCGTAAGGCAt < 1:2448407/69‑1 (MQ=255) cagccagcTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGGCGCACGGAATACCGTTTCGTAAGGCAt < 1:92705/69‑1 (MQ=255) agcTTATAGTTGCCTGGCCGTCGTCGATTTCTAGTGGCGCACGGAATACCGTTTCGTAAGGCATCTTTg < 1:3246141/69‑1 (MQ=255) agcTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGGCGCACGGAATACCGTTTCGTAAGGCATCTTTg < 1:761628/69‑1 (MQ=255) agcTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGGCGCACGGAATACCGTTTCGTAAGGCATCTTTg < 1:990863/69‑1 (MQ=255) agcTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGGCGCACGGAATACCGTTTCGTAAGGCATCTTTg < 1:3307531/69‑1 (MQ=255) agcTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGGCGCACGGAATACCGTTTCGTAAGGCATCTTTg < 1:3007849/69‑1 (MQ=255) agcTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGGCGCACGGAATACCGTTTCGTAAGGCATCTTTg < 1:2662141/69‑1 (MQ=255) agcTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGGCGCACGGAATACCGTTTCGTAAGGCATCTTTg < 1:2658128/69‑1 (MQ=255) agcTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGGCGCACGGAATACCGTTTCGTAAGGCATCTTTg < 1:2535579/69‑1 (MQ=255) agcTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGGCGCACGGAATACCGTTTCGTAAGGCATCTTTg < 1:2305104/69‑1 (MQ=255) agcTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGGCGCACGGAATACCGTTTCGTAAGGCATCTTTg < 1:2156489/69‑1 (MQ=255) agcTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGGCGCACGGAATACCGTTTCGTAAGGCATCTTTg < 1:1470698/69‑1 (MQ=255) agcTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGGCGCACGGAATACCGTTTCGTAAGGCATCTTTg < 1:1187529/69‑1 (MQ=255) agcTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGGCGCACGGAATACCGTTTCGTAAGGCATCTTTg < 1:1047671/69‑1 (MQ=255) ttATAGTTGCCTGGCCGTAGGCGATTTCTAGTGGCGCACgg < 1:455171/41‑1 (MQ=255) | TTGGCTTTAACGTCAATATTACGCCCATCCAGCTGGAAACCTTTGTTATTGTTCAGCCAGCTTATAGTTGCCTGGCCGTCGGCGATTTCTAGTGGCGCACGGAATACCGTTTCGTAAGGCATCTTTG > minE/2210114‑2210240 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |