| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,214,266 | G→A | 58.1% | A332V (GCT→GTT) | mreC ← | cell wall structural complex MreBCD transmembrane component MreC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,214,266 | 0 | G | A | 58.1% | 5.4 / 19.9 | 31 | A332V (GCT→GTT) | mreC | cell wall structural complex MreBCD transmembrane component MreC |
| Reads supporting (aligned to +/- strand): ref base G (2/11); new base A (6/12); total (8/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.12e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.30e-01 | |||||||||||
GCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCCGGTTCA > minE/2214212‑2214325 | gCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGCTGc < 1:901206/59‑1 (MQ=255)gCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGCTGc < 1:2048911/59‑1 (MQ=255)gCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGCTGc < 1:2175407/59‑1 (MQ=255)gCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGCTGc < 1:3416409/59‑1 (MQ=255)gCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGCTGc < 1:324462/59‑1 (MQ=255) tgtgGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGt > 1:120451/1‑69 (MQ=255) tgGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGt < 1:2185799/67‑1 (MQ=255) tgGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGt < 1:2999717/67‑1 (MQ=255) gagCGATTAGCAGCGGGCTGTGTGGGCGCAGCAGGCGCAGTAACTGCATTTcc < 1:2552050/53‑1 (MQ=255) gagCGATTAGCAGCGGGCTGTGTGGGCGCAGCAGGCGCAGTAACTGCATTTcc < 1:562978/53‑1 (MQ=255) gagCGATTAGCAGCGGGCTGTGTGGGCGCAGCAGGCGCAGTAACTGCATTTcc < 1:400657/53‑1 (MQ=255) gagCGATTAGCAGCGGGCTGTGTGGGCGCAGCAGGCGCAGTAACTGCATTTcc < 1:390920/53‑1 (MQ=255) gagCGATTAGCAGCGGGCTGTGTGGGCGCAGCAGGCGCAGTAACTGCATTTcc < 1:380958/53‑1 (MQ=255) gagCGATTAGCAGCGGGCTGTGTGGGCGCAGCAGGCGCAGTAACTGCATTTcc < 1:338777/53‑1 (MQ=255) gagCGATTAGCAGCGGGCTGTGTGGGCGCAGCAGGCGCAGTAACTGCATTTcc < 1:2542801/53‑1 (MQ=255) gagCGATTAGCAGCGGGCTGTGTGGGCGCAGCAGGCGCAGTAACTGCATTTcc < 1:2347449/53‑1 (MQ=255) gagCGATTAGCAGCGGGCTGTGTGGGCGCAGCAGGCGCAGTAACTGCATTTcc < 1:2055366/53‑1 (MQ=255) gagCGATTAGCAGCGGGCTGTGTGGGAGCAGCAGGCGCAGTAACTGCATTTcc < 1:2380940/53‑1 (MQ=255) gagCGATTAGCAGCGGGCTGTGTAGGCGCAGCAGGCGCAGTAACTGCATTTcc < 1:2292368/53‑1 (MQ=255) agCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGTtgctg > 1:2139044/1‑67 (MQ=255) agcagcGGGCTGTGTGGGCGCAGCAGGCGCAGTAACTGCATTTcc < 1:2689434/45‑1 (MQ=255) gtgggCGCAGCAGGCGCAGTAACTGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGTGATcccc > 1:258192/4‑69 (MQ=255) gtgggCGCAGCAGGCGCAGTAACTGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGTGATcccc > 1:899512/4‑69 (MQ=255) gtgggCGCAGCAGGCGCAGTAACTGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGTGATcccc > 1:2803706/4‑69 (MQ=255) gtgggCGCAGCAGGCGCAGTAACTGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGTGATcccc > 1:2244299/4‑69 (MQ=255) gtgggCGCAGCAGGCGCAGTAACTGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGTGATcccc > 1:2183825/4‑69 (MQ=255) gtgggCGCAGCAGGCGCAGTAACTGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGTGATcccc > 1:862252/4‑69 (MQ=255) tCGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTg < 1:182082/58‑1 (MQ=255) ggCGCAGTAGCTGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCCGGTTCa < 1:1348586/69‑1 (MQ=255) ggCGCAGTAGCTGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCCGGTTCa < 1:2733450/69‑1 (MQ=255) gagcAGTAGCTGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCCGGTTCa < 1:2455134/66‑1 (MQ=255) | GCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCCGGTTCA > minE/2214212‑2214325 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |