| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,217,619 | A→G | 54.3% | V332V (GTT→GTC) | yhdA ← | conserved inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,217,619 | 0 | A | G | 54.3% | 8.2 / 46.8 | 59 | V332V (GTT→GTC) | yhdA | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base A (7/20); new base G (14/18); total (21/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.82e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGCCAGGCGCAGATACCAATGTGGATCATATCGTCGCGATCGAGCATTTTATTGTTCGGCAAGGTATCAACGGCTTTGATTAACTGACCGGCGATGCTCTCTGCCTCTTTTAACGTCCGGTGCGGTAACAGCGCAG > minE/2217551‑2217686 | cGCCAGGCGCAGATACCAATGTGGATCATATCGTCGCGATCGAGCATTTTATTGTTCGGCAAGGTATCa > 1:2197534/1‑69 (MQ=255)cGCCAGGCGCAGATACCAATGTGGATCATATCGTCGCGATCGAGCATTTTATTGTTCGGCAAGGTATCa > 1:304308/1‑69 (MQ=255)cGCCAGGCGCAGATACCAATGTGGATCATATCGTCGCGATCGAGCATTTTATTGTTCGGCAAGGTATCa > 1:1110916/1‑69 (MQ=255)cGCCAGGCGCAGATACCAATGTGGATCATATCGTCGCGATCGAGCATTTTATTGTTCGGCAAGGTATCa > 1:1230950/1‑69 (MQ=255)cGCCAGGCGCAGATACCAATGTGGATCATATCGTCGCGATCGAGCATTTTATTGTTCGGCAAGGTATCa > 1:2955774/1‑69 (MQ=255)cGCCAGGCGCAGATACCAATGTGGATCATATCGTCGCGATCGAGCATTTTATTGTTCGGCAAGGTATCa > 1:2732849/1‑69 (MQ=255)cGCCAGGCGCAGATACCAATGTGGATCATATCGTCGCGATCGAGCATTTTATTGTTCGGCAAGGTATCa > 1:2201457/1‑69 (MQ=255)cGCCAGGCGCAGATACCAATGTGGATCATATCGTCGCGATCGAGCATTTTATTGTTCGGCAAGGTATCa > 1:2322249/1‑69 (MQ=255) cgcAGATACCAATGTGGATCATATCGTCGCGATCGAGCATTTTATTGTTCGGCAAGGTATCAACGGCt > 1:333303/1‑68 (MQ=255) gaaGATACCAATGTGGATCATATCGTCGCGATCGAGCATTTTATTGTTCGGCAAGGTATCAACGGCttt < 1:2420649/67‑1 (MQ=255) aTACCAATGTGGATCATATCGTCGCGATCGAGCATTTTATTGTTCGGCAAGGTATCAACGGCTTTGAtt < 1:980103/69‑1 (MQ=255) aCCAATGTGGATCATATCGTCGCGATCGAGCATTTTATTGTTCGGCAAGGTATCGACGGCTTTGATTaa > 1:2465533/1‑69 (MQ=255) aCCAATGTGGATCATATCGTCGCGATCGAGCATTTTATTGTTCGGCAAGGTATCGACGGCTTTGATTaa > 1:654389/1‑69 (MQ=255) aCCAATGTGGATCATATCGTCGCGATCGAGCATTTTATTGTTCGGCAAGGTATCGACGGCTTTGATTaa > 1:613287/1‑69 (MQ=255) aCCAATGTGGATCATATCGTCGCGATCGAGCATTTTATTGTTCGGCAAGGTATCGACGGCTTTGATTaa > 1:2618708/1‑69 (MQ=255) aCCAATGTGGATCATATCGTCGCGATCGAGCATTTTATTGTTCGGCAAGGTATCGACGGCTTTGATTaa > 1:69644/1‑69 (MQ=255) aCCAATGTGGATCATATCGTCGCGATCGAGCATTTTATTGTTCGGCAAGGTATCGACGGCTTTGATTaa > 1:503314/1‑69 (MQ=255) aCCAATGTGGATCATATCGTCGCGATCGAGCATTTTATTGTTCGGCAAGGTATCGACGGCTTTGATTaa > 1:3258640/1‑69 (MQ=255) aCCAATGTGGATCATATCGTCGCGATCGAGCATTTTATTGTTCGGCAAGGTATCGACGGCTTTGATTaa > 1:699252/1‑69 (MQ=255) aCCAATGTGGATCATATCGTCGCGATCGAGCATTTTATTGTTCGGCAAGGTATCGACGGCTTTGATTaa > 1:94570/1‑69 (MQ=255) aCCAATGTGGATCATATCGTCGCGATCGAGCATTTTATTGTTCGGCAAGGTATCGACGGCTTTGATTaa > 1:846156/1‑69 (MQ=255) aCCAATGTGGATCATATCGTCGCGATCGAGCATTTTATTGTTCGGCAAGGTATCGACGGCTTTGATTaa > 1:1256353/1‑69 (MQ=255) aCCAATGTGGATCATATCGTCGCGATCGAGCATTTTATTGTTCGGCAAGGTATCGACGGCTTTGATTaa > 1:936984/1‑69 (MQ=255) aCCAATGTGGATCATATCGTCGCGATCGAGCATTTTATTGTTCGGCAAGGTATCGACGGCTTTGATTaa > 1:900189/1‑69 (MQ=255) aCCAATGTGGATCATATCGTCGCGATCGAGCATTTTATTGTTCGGCAAGGTATCGACGGCTTTGATTaa > 1:1410354/1‑69 (MQ=255) aCCAATGTGGATCATATCGTCGCGATCGAGCATTTTATTGTTCGGCAAGGTATCAACGGCTTTGATTaa > 1:3331023/1‑69 (MQ=255) aCCAATGTGGATCATATCGTCGCGATCGAGCATTTTATTGTTCGGCAAGGTATCAACGGCTTTGATTa > 1:349857/1‑68 (MQ=255) aaTGTGTATCATATCGTCGCGATCGAGCATTTTATTGTTCGGCAAGGTATCGACGGCTTTGATTAACt < 1:1023593/68‑1 (MQ=255) aaTGTGGATCATATCGTCGCGATCGATCATTTTATTGTTCGGCAAGGTATCGACGGCTTTGATTAACt < 1:690668/68‑1 (MQ=255) aaTGTGGATCATATCGTCGCGATCGAGCATTTTATTGTTCGGCAAGGTATCGACGGCTTTGATTAACt < 1:712431/68‑1 (MQ=255) aaTGTGGATCATATCGTCGCGATCGAGCATTTTATTGTTCGGCAAGGTATCGACGGCTTTGATTAACt < 1:124343/68‑1 (MQ=255) aaTGTGGATCATATCGTCGCGATCGAGCATTTTATTGTTCGGCAAGGTATCGACGGCTTTGATTAACt < 1:428778/68‑1 (MQ=255) aaTGTGGATCATATCGTCGCGATCGAGCATTTTATTGTTCGGCAAGGTATCGACGGCTTTGATTAACt < 1:2630656/68‑1 (MQ=255) aaTGTGGATCATATCGTCGCGATCGAGCATTTTATTGTTCGGCAAGGTATCGACGGCTTTGATTAACt < 1:922758/68‑1 (MQ=255) aaTGTGGATCATATCGTCGCGATCGAGCATTTTATTGTTCGGCAAGGTATCGACGGCTTTGATTAACt < 1:1542725/68‑1 (MQ=255) aaTGTGGATCATATCGTCGCGATCGAGCATTTTATTGTTCGGCAAGGTATCGACGGCTTTGATTAACt < 1:250481/68‑1 (MQ=255) aaTGTGGATCATATCGTCGCGATCGAGCATTTTATTGTTCGGCAAGGTATCGACGGCTTTGATTAACt < 1:1587354/68‑1 (MQ=255) aaTGTGGATCATATCGTCGCGATCGAGCATTTTATTGTTCGGCAAGGTATCGACGGCTTTGATTAACt < 1:2423475/68‑1 (MQ=255) aaTGTGGATCATATCGTCGCGATCGAGCATTTTATTGTTCGGCAAGGTATCGACGGCTTTGATTAACt < 1:1614270/68‑1 (MQ=255) aaTGTGGATCATATCGTCGCGATCGAGCATTTTATTGTTCGGCAAGGTATCGACGGCTTTGATTAACt < 1:2420251/68‑1 (MQ=255) aaTGTGGATCATATCGTCGCGATCGAGCATTTTATTGTTCGGCAAGGTATCGACGGCTTTGATTAACt < 1:2236978/68‑1 (MQ=255) aaTGTGGATCATATCGTCGCGATCGAGCATTTTATTGTTCGGCAAGGTATCGACGGCTTTGATTAACt < 1:716448/68‑1 (MQ=255) aaTGTGGATCATATCGTCGCGATCGAGCATTTTATTGTTCGGCAAGGTATCAACGGCTTTGAtt < 1:7380/64‑1 (MQ=255) aaTGTGGATCATATCGTCGCGATCGAGCATTTTATTGTTCGGCAAGGTATCAACGGCTTTGATTAACt < 1:2769940/68‑1 (MQ=255) aaTGTGGATCATATCGTCGCGATCGAGCATTTTATTGTTCGGCAAGGTATCAACGGCTTTGATTAACt < 1:1043311/68‑1 (MQ=255) aaTGTGGATCATATCGTCGCGATCGAGCATTTTATTGTTCGGCAAGGTATCAACGGCTTTGATTAACt < 1:1665448/68‑1 (MQ=255) aaTGTGGATCATATCGTCGCGATCGAGCATTTTATTGTTAGGCAAGGTATCGACGGCTTTGATTAACt < 1:2022251/68‑1 (MQ=255) aTGTGGATCATATCGTCGCGATCGAGCATTTTATTGTTCGGCAAGGTATCGACGGCTTTGATTAACt < 1:2440388/67‑1 (MQ=255) gATCATATCGTCGCGATCGAGCATTTTATTGTTCGGCAAGGTATCAACGGCTTTGATTAACTGACCGGc < 1:902589/69‑1 (MQ=255) gATCATATCGTCGCGATAGAGCATTTTATTGTTCGGCAAGGTATCAACGGCTTTGATTAACTGACCGGc < 1:2577109/69‑1 (MQ=255) aTCATATCGTCGCGATCGAGCATTTTATTGTTCGGCAAGGTATCGACGGCTTTGATTAACt < 1:938957/61‑1 (MQ=255) atCGTCGCGATCGAGCATTTTATTGTTCGGCAAGGTATCAACGGCTTTGATTAACTGACCGGCGATGct < 1:2629802/69‑1 (MQ=255) atCGTCGCGATCGAGCATTTTATTGTTCGGCAAGGTATCAACGGCTTTGATTAACTGACCGGCGATGct < 1:728442/69‑1 (MQ=255) atCGTCGCGATCGAGCATTTTATTGTTCGGCAAGGTATCAACGGCTTTGATTAACTGACCGGCGATGct < 1:1784126/69‑1 (MQ=255) atCGTCGCGATCGAGCATTTTATTGTTCGGCAAGGTATCAACGGCTTTGATTAACTGACCGGCGATGct < 1:1972442/69‑1 (MQ=255) atCGTCGCGATCGAGCATTTTATTGTTCGGCAAGGTATCAACGGCTTTGATTAACTGACCGGCGATGct < 1:2269546/69‑1 (MQ=255) atCGTCGCGATCGAGCATTTTATTGTTCGGCAAGGTATCAACGGCTTTGATTAACTGACCGGCGATGct < 1:1635988/69‑1 (MQ=255) atCGTCGCGATCGAGCATTTTATTGTTCGGCAAGGTATCAACGGCTTTGATTAACTGACCGGCGATGct < 1:1361947/69‑1 (MQ=255) atCGTCGCGATCGAGCATTTTATTGTTCGGCAAGGTATCAACGGCTTTGATTAACTGACCGGCGATGct < 1:2856701/69‑1 (MQ=255) tcgtcgCGATCGAGCATTTTATTGTTCGGCAAGGTATCAACGGCTTTGATTAACTGACCGGCGATGct < 1:366788/68‑1 (MQ=255) cgcgATCGAGCATTTTATTGTTCGGCAAGGTATCAACGGCTTTGATTAACTGACCGGCGATGCTCTCTg > 1:1497102/1‑69 (MQ=255) gATCGAGCATTTTATTGTTCGGCAAGGTATCAACGGCTTTGATTa < 1:489997/45‑1 (MQ=255) gATCGAGCATTTTATTGTTCGGCAAGGTATCAACGGCTTTGATTa < 1:3246064/45‑1 (MQ=255) gATCGAGCATTTTATTGTTCGGCAAGGTATCAACGGCTTTGATTa < 1:306763/45‑1 (MQ=255) ttATTGTTCGGCAAGGTATCAACGGCTTTGATTAACTGACCGGCGATGCTCTCTGCCTCTTTTAACGTc > 1:2950732/1‑69 (MQ=255) ttATTGTTCGGCAAGGTATCAACGGCTTTGATTAACTGACCGGCGATGCTCTCTGCCTCTTTTAACGTc > 1:3435812/1‑69 (MQ=255) ttATTGTTCGGCAAGGTATCAACGGCTTTGATTAACTGACCGGCGATGCTCTCTGCCTCTTTTAACGTc > 1:490641/1‑69 (MQ=255) aaCGGCTTTGATTAACTGACCGGCGATGCTCTCTGCCTCTTTTAACGTCCGGTGCGGTAAc > 1:1564192/1‑61 (MQ=255) aaCGGCTTTGATTAACTGACCGGCGATGCTCTCTGCCTCTTTTAACGTCCGGTGCGGTAAc > 1:478418/1‑61 (MQ=255) aaCGGCTTTGATTAACTGACCGGCGATGCTCTCTGCCTCTTTTAACGTCCGGTGCGGTAAc > 1:467218/1‑61 (MQ=255) aaCGGCTTTGATTAACTGACCGGCGATGCTCTCTGCCTCTTTTAACGTCCGGTGCGGTAAc > 1:2701301/1‑61 (MQ=255) aaCGGCTTTGATTAACTGACCGGCGATGCTCTCTGCCTCTTTTAACGTCCGGTGCGGTAACAGCgcag > 1:2455602/1‑68 (MQ=255) aaCGGCTTTGATTAACTGACCGGCGATGCTCTCTGCCTCTTTTAACGTCCGGTGCGGTAACAGCgcag > 1:1055134/1‑68 (MQ=255) aaCGGCTTTGATTAACTGACCGGCGATGCTCTCTGCCTCTTTTAACGTCCGGTGCGGTAACAGCgcag > 1:3196702/1‑68 (MQ=255) | CGCCAGGCGCAGATACCAATGTGGATCATATCGTCGCGATCGAGCATTTTATTGTTCGGCAAGGTATCAACGGCTTTGATTAACTGACCGGCGATGCTCTCTGCCTCTTTTAACGTCCGGTGCGGTAACAGCGCAG > minE/2217551‑2217686 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |