Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,218,437 C→T 45.0% V60I (GTA→ATA) ‡ yhdA ← conserved inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,218,4370CT45.0% 23.7 / 81.2 102V60I (GTA→ATA) ‡yhdAconserved inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (39/17);  new base T (22/24);  total (61/41)
Fisher's exact test for biased strand distribution p-value = 2.81e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 114 total aligned reads displayed.

TCTACACGAACGATATCTGCCGACATCATTAATTCGGTAATTTGTGGCCTTAATACGCTGAAGTCATTCGACACAAGGTGGGTATCAATCGCCGTCGCCACCGCCTGAACGCGATGACTAAACT  >  minE/2218382‑2218505
                                                       |                                                                    
tCTACACGAACGATATCTGCCGACATCATTAATTCGGTAATTTGTGGCCTTAATACGCTGAAGTCATTc                                                         <  1:2765130/69‑1 (MQ=255)
tCTACACGAACGATATCTGCCGACATCATTAATTCGGTAATTTGTGGCCTTAATACGCTGAAGTCATTc                                                         <  1:974752/69‑1 (MQ=255)
tCTACACGAACGATATCTGCCGACATCATTAATTCGGTAATTTGTGGCCTTAATACGCTGAAGTCATTc                                                         <  1:959186/69‑1 (MQ=255)
tCTACACGAACGATATCTGCCGACATCATTAATTCGGTAATTTGTGGCCTTAATACGCTGAAGTCATTc                                                         <  1:601504/69‑1 (MQ=255)
tCTACACGAACGATATCTGCCGACATCATTAATTCGGTAATTTGTGGCCTTAATACGCTGAAGTCATTc                                                         <  1:1086041/69‑1 (MQ=255)
tCTACACGAACGATATCTGCCGACATCATTAATTCGGTAATTTGTGGCCTTAATACGCTGAAGTCATTc                                                         <  1:2679391/69‑1 (MQ=255)
tCTACACGAACGATATCTGCCGACATCATTAATTCGGTAATTTGTGGCCTTAATACGCTGAAGTCATTc                                                         <  1:2228627/69‑1 (MQ=255)
tCTACACGAACGATATCTGCCGACATCATTAATTCGGTAATTTGTGGCCTTAATACGCTGAAGTCATTc                                                         <  1:1426474/69‑1 (MQ=255)
tCTACACGAACGATATCTGCCGACATCATTAATTCGGTAATTTGTGGCCTTAATACGCTGAAGTCATTc                                                         <  1:775785/69‑1 (MQ=255)
tCTACACGAACGATATCTGCAGACATCATTAATTCGGTAATTTGTGGCCTTAATACGCTGAAGTCATTc                                                         <  1:3208192/69‑1 (MQ=255)
 cTACACGAACGATATCTGCCGACATCATTAATTCGGTAATTTGTGGCCTTAATACGCTGAAGTCa                                                            >  1:1570200/1‑65 (MQ=255)
 cTACACGAACGATATCTGCCGACATCATTAATTCGGTAATTTGTGGCCTTAATACGCTGAAGTCa                                                            >  1:355600/1‑65 (MQ=255)
 cTACACGAACGATATCTGCCGACATCATTAATTCGGTAATTTGTGGCCTTAATACGCTGAAGTCATTc                                                         >  1:100020/1‑68 (MQ=255)
 cTACACGAACGATATCTGCCGACATCATTAATTCGGTAATTTGTGGCCTTAATACGCTGAAGTCATTc                                                         >  1:363603/1‑68 (MQ=255)
 cTACACGAACGATATCTGCCGACATCATTAATTCGGTAATTTGTGGCCTTAATACGCTGAAGTCATTc                                                         >  1:1833285/1‑68 (MQ=255)
 cTACACGAACGATATCTGCCGACATCATTAATTCGGTAATTTGTGGCCTTAATACGCTGAAGTCATTc                                                         >  1:3225550/1‑68 (MQ=255)
 cTACACGAACGATATCTGCCGACATCATTAATTCGGTAATTTGTGGCCTTAATACGCTGAAGTCATTc                                                         >  1:77864/1‑68 (MQ=255)
 cTACACGAACGATATCTGCCGACATCATTAATTCGGTAATTTGTGGCCTTAATACGCTGAAGTCATTc                                                         >  1:2626970/1‑68 (MQ=255)
 cTACACGAACGATATCTGCCGACATCATTAATTCGGTAATTTGTGGCCTTAATACGCTGAAGTCATTc                                                         >  1:2949597/1‑68 (MQ=255)
   acacGAACGATATCTGCCGACATCATTAATTCGGTAATTTGTGGCCTTAATACGCTGAAGTCATTCGa                                                       >  1:2861683/1‑68 (MQ=255)
          cgaTATCTGCCGACATCATTAATTCGGTAATTTGTGGCCTTAATGTGCTGAAGTCATTCGACACAAGGt                                               <  1:3240609/69‑1 (MQ=255)
          cgaTATCTGCCGACATCATTAATTCGGTAATTTGTGGCCTTAATGTGCTGAAGTCATTCGACACAAGGt                                               <  1:2380204/69‑1 (MQ=255)
          cgaTATCTGCCGACATCATTAATTCGGTAATTTGTGGCCTTAATGTGCTGAAGTCATTCGACACAAGGt                                               <  1:2958598/69‑1 (MQ=255)
          cgaTATCTGCCGACATCATTAATTCGGTAATTTGTGGCCTTAATGTGCTGAAGTCATTCGACACAAGGt                                               <  1:334860/69‑1 (MQ=255)
          cgaTATCTGCCGACATCATTAATTCGGTAATTTGTGGCCTTAATGTGCTGAAGTCATTCGACACAAGGt                                               <  1:2730532/69‑1 (MQ=255)
          cgaTATCTGCCGACATCATTAATTCGGTAATTTGTGGCCTTAATGTGCTGAAGTCATTCGACACAAGGt                                               <  1:2140237/69‑1 (MQ=255)
          cgaTATCTGCCGACATCATTAATTCGGTAATTTGTGGCCTTAATGTGCTGAAGTCATTCGACACAAGGt                                               <  1:2800182/69‑1 (MQ=255)
          cgaTATCTGCCGACATCATTAATTCGGTAATTTGTGGCCTTAATGTGCTGAAGTCATTCGACACAAGGt                                               <  1:497809/69‑1 (MQ=255)
          cgaTATCTGCCGACATCATTAATTCGGTAATTTGTGGCCTTAATGTGCTGAAGTCATTCGACACAAGGt                                               <  1:1179905/69‑1 (MQ=255)
          cgaTATCTGCCGACATCATTAATTCGGTAATTTGTGGCCTTAATGTGCTGAAGTCATTCGACACAAGGt                                               <  1:1349853/69‑1 (MQ=255)
          cgaTATCTGCCGACATCATTAATTCGGTAATTTGTGGCCTTAATGTGCTGAAGTCATTCGACACAAGGt                                               <  1:69604/69‑1 (MQ=255)
          cgaTATCTGCCGACATCATTAATTCGGTAATTTGTGGCCTTAATGTGCTGAAGTCATTCGACACAAGGt                                               <  1:680331/69‑1 (MQ=255)
          cgaTATCTGCCGACATCATTAATTCGGTAATTTGTGGCCTTAATGTGCTGAAGTCATTCGACACAAGGt                                               <  1:1547014/69‑1 (MQ=255)
          cgaTATCTGCCGACATCATTAATTCGGTAATTTGTGGCCTTAATGTGCTGAAGTCATTCGACACAAGGt                                               <  1:640139/69‑1 (MQ=255)
          cgaTATCTGCCGACATCATTAATTCGGTAATTTGTGGCCTTAATGTGCTGAAGTCATTCGACACAAGGt                                               <  1:978396/69‑1 (MQ=255)
          cgaTATCTGCCGACATCATTAATTCGGTAATTTGTGGCCTTAATGTGCTGAAGTCATTCGACACAAGGt                                               <  1:1006553/69‑1 (MQ=255)
          cgaTATCTGCCGACATCATTAATTCGGTAATTTGTGGCCTTAATGTGCTGAAGTCATTCGACACAAGGt                                               <  1:1179656/69‑1 (MQ=255)
          cgaTATCTGCCGACATCATTAATTCGGTAATTTGTGGCCTTAATGTGCTGAAATCATTCGACACAAGGt                                               <  1:2632509/69‑1 (MQ=255)
           gaTATCTGCCGACATCATTAATTCGGTAATTTGTGGCCTTAATGTGCTGAAGTCATTCGACACAAGGt                                               <  1:2311678/68‑1 (MQ=255)
                 tGCCGACATCATTAATTCTGTAATTTGTGGCCTTAATACGCTGAAGTCATTCGACACAAGGTg                                              >  1:2005603/1‑63 (MQ=255)
                 tGCCGACATCATTAATTCGGTAATTTGTGGCCTTAATACGCTGAAGTCATTCGACACAAGGTg                                              >  1:2037668/1‑63 (MQ=255)
                 tGCCGACATCATTAATTCGGTAATTTGTGGCCTTAATACGCTGAAGTCATTCGACACAAGGTg                                              >  1:2199330/1‑63 (MQ=255)
                 tGCCGACATCATTAATTCGGTAATTTGTGGCCTTAATACGCTGAAGTCATTCGACACAAGGTg                                              >  1:3272757/1‑63 (MQ=255)
                     gACATCATTAATTCGGTAATTTGTGGCCTTAATACGCTGAAGTCATTCGACACAAGGTGGGTATCa                                       <  1:388507/66‑1 (MQ=255)
                     gACATCATTAATTCGGTAATTTGTGGCCTTAATACGCTGAAGTCATTCGACACAAGGTGGGTATCa                                       <  1:2154650/66‑1 (MQ=255)
                        atcatTAATTCGGTAATTTGTGGCCTTAATACGCTGAAGt                                                              >  1:3137639/1‑40 (MQ=255)
                        atcatTAATTCGGTAATTTGTGGCCTTAATACGCTGAAGt                                                              >  1:1137610/1‑40 (MQ=255)
                        atcatTAATTCGGTAATTTGTGGCCTTAATACGCTGAAGt                                                              >  1:1417021/1‑40 (MQ=255)
                        atcatTAATTCGGTAATTTGTGGCCTTAATACGCTGAAGt                                                              >  1:533168/1‑40 (MQ=255)
                        atcatTAATTCGGTAATTTGTGGCCTTAATACGCTGAAGt                                                              >  1:183866/1‑40 (MQ=255)
                        atcatTAATTCGGTAATTTGTGGCCTTAATACGCTGAAGt                                                              >  1:1920912/1‑40 (MQ=255)
                        atcatTAATTCGGTAATTTGTGGCCTTAATACGCTGAAGt                                                              >  1:1874124/1‑40 (MQ=255)
                             tAATTCGGTAATTTGTGGCCTTAATACGCTGAAGTCATTCGACACAAGGTGGGTATCAATCGCCGTCGc                            <  1:35655/69‑1 (MQ=255)
                                  cGGTAATTTGTGGCCTTAATGTGCTGAAGTCATTCGACACAAGGTAGGTAt                                         >  1:950765/1‑51 (MQ=255)
                                  cGGTAATTTGTGGCCTTAATGTGCTGAAGTCATTCGACACAAGGTAGGTAt                                         >  1:1001161/1‑51 (MQ=255)
                                  cGGTAATTTGTGGCCTTAATGTGCTGAAGTCATTCGACACAAGGTAGGTAt                                         >  1:861138/1‑51 (MQ=255)
                                  cGGTAATTTGTGGCCTTAATGTGCTGAAGTCATTCGACACAAGGTAGGTAt                                         >  1:843756/1‑51 (MQ=255)
                                  cGGTAATTTGTGGCCTTAATGTGCTGAAGTCATTCGACACAAGGTAGGTAt                                         >  1:311041/1‑51 (MQ=255)
                                  cGGTAATTTGTGGCCTTAATGTGCTGAAGTCATTCGACACAAGGTAGGTAt                                         >  1:443985/1‑51 (MQ=255)
                                  cGGTAATTTGTGGCCTTAATGTGCTGAAGTCATTCGACACAAGGTAGGTAt                                         >  1:509408/1‑51 (MQ=255)
                                  cGGTAATTTGTGGCCTTAATGTGCTGAAGTCATTCGACACAAGGTAGGTAt                                         >  1:1025260/1‑51 (MQ=255)
                                  cGGTAATTTGTGGCCTTAATGTGCTGAAGTCATTCGACACAAGGTAGGTAt                                         >  1:128183/1‑51 (MQ=255)
                                  cGGTAATTTGTGGCCTTAATGTGCTGAAGTCATTCGACACAAGGTAGGTAt                                         >  1:1696861/1‑51 (MQ=255)
                                  cGGTAATTTGTGGCCTTAATGTGCTGAAGTCATTCGACACAAGGTAGGTAt                                         >  1:1175000/1‑51 (MQ=255)
                                  cGGTAATTTGTGGCCTTAATGTGCTGAAGTCATTCGACACAAGGTAGGTAt                                         >  1:1909716/1‑51 (MQ=255)
                                  cGGTAATTTGTGGCCTTAATGTGCTGAAGTCATTCGACACAAGGTAGGTAt                                         >  1:114179/1‑51 (MQ=255)
                                  cGGTAATTTGTGGCCTTAATGTGCTGAAGTCATTCGACACAAGGTAGGTAt                                         >  1:1939054/1‑51 (MQ=255)
                                  cGGTAATTTGTGGCCTTAATGTGCTGAAGTCATTCGACACAAGGTAGGTAt                                         >  1:2004106/1‑51 (MQ=255)
                                  cGGTAATTTGTGGCCTTAATGTGCTGAAGTCATTCGACACAAGGTAGGTAt                                         >  1:2146149/1‑51 (MQ=255)
                                  cGGTAATTTGTGGCCTTAATGTGCTGAAGTCATTCGACACAAGGTAGGTAt                                         >  1:215156/1‑51 (MQ=255)
                                  cGGTAATTTGTGGCCTTAATGTGCTGAAGTCATTCGACACAAGGTAGGTAt                                         >  1:2310832/1‑51 (MQ=255)
                                  cGGTAATTTGTGGCCTTAATGTGCTGAAGTCATTCGACACAAGGTAGGTAt                                         >  1:2673328/1‑51 (MQ=255)
                                  cGGTAATTTGTGGCCTTAATGTGCTGAAGTCATTCGACACAAGGTAGGTAt                                         >  1:1075429/1‑51 (MQ=255)
                                  cGGTAAATTGTGGCCTTAATGTGCTGAAGTCATTCGACACAAGGTAGGTAt                                         >  1:1077/1‑51 (MQ=255)
                                    gTAATTTGTGGCCTTAATACGCTGAAGTCATTCGACACAAGGTGGGTATCAATCGCCGTCGCCACCGc                      <  1:241013/68‑1 (MQ=255)
                                    gTAATTTGTGGCCTTAATACGCTGAAGTCATTCGACACAAGGTGGGTATCAATCGCCGTCGCCACCGc                      <  1:2094789/68‑1 (MQ=255)
                                          tgtgGCCTTAATACGCTGAAGTCATTCGATACAAGGTGGGTATCAATCGCCGTCGCCACCGCCTGAAc                >  1:1655091/1‑68 (MQ=255)
                                          tgtgGCCTTAATACGCTGAAGTCATTCGACACAAGGTGGGTATCAATCGCCGTCGCCACCGCCTGAAc                >  1:2445697/1‑68 (MQ=255)
                                          tgtgGCCTTAATACGCTGAAGTCATTCGACACAAGGTGGGTATCAATCGCCGTCGCCACCGCCTGAAc                >  1:693533/1‑68 (MQ=255)
                                          tgtgGCCTTAATACGCTGAAGTCATTCGACACAAGGTGGGTATCAATCGCCGTCGCCACCGCCTGAAc                >  1:2031417/1‑68 (MQ=255)
                                          tgtgGCCTTAATACGCTGAAGTCATTCGACACAAGGTGGGTATCAATCGCCGTCGCCACCGCCTGAAc                >  1:315889/1‑68 (MQ=255)
                                          tgtgGCCTTAATACGCTGAAGTCATTCGACACAAGGTGGGTATCAATCGCCGTCGCCACCGCCTGAAc                >  1:1079710/1‑68 (MQ=255)
                                          tgtgGCCTTAATACGCTGAAGTCATTCGACACAAGGTGGGTATCAATCGCCGTCGCCACCGCCTGAAc                >  1:2567925/1‑68 (MQ=255)
                                              gCCTTAATACGCTGAAGTCATTCGACACAAGGTGGGTATCAATCGCCGTCGGCACCGCCTGAACGCGAt           <  1:1342664/69‑1 (MQ=255)
                                                cTTAATACGCTGAAGTCATTCGACACAAGGTGGGTATCAATCGCCGTCGCCACCGCCTGAACGCGATg          >  1:1313194/1‑68 (MQ=255)
                                                cTTAATACGCTGAAGTCATTCGACACAAGGTGGGTATCAATCGCCGTCGCCACCGCCTGAACGCGATg          >  1:2458945/1‑68 (MQ=255)
                                                  tAATACGCTGAAGTCATTCGACACAAGGTGGGTATCAATCGCCGTCGcc                           >  1:1562690/1‑49 (MQ=255)
                                                  tAATACGCTGAAGTCATTCGACACAAGGTGGGTATCAATCGCCGTCGcc                           >  1:3303189/1‑49 (MQ=255)
                                                  tAATACGCTGAAGTCATTCGACACAAGGTGGGTATCAATCGCCGTCGc                            >  1:879714/1‑48 (MQ=255)
                                                       cGCTGAAGTCATTCGACACAAGGTGGGTATCAATCGCCGTCGCCACCGCCTGAACGCGATGACTAAACt  <  1:45208/69‑1 (MQ=255)
                                                       cGCTGAAGTCATTCGACACAAGGTGGGTATCAATCGCCGTCGCCACCGCCTGAACGCGATGACTAAACt  <  1:723370/69‑1 (MQ=255)
                                                       cGCTGAAGTCATTCGACACAAGGTGGGTATCAATCGCCGTCGCCACCGCCTGAACGCGATGACTAAACt  <  1:703291/69‑1 (MQ=255)
                                                       cGCTGAAGTCATTCGACACAAGGTGGGTATCAATCGCCGTCGCCACCGCCTGAACGCGATGACTAAACt  <  1:1527729/69‑1 (MQ=255)
                                                       cGCTGAAGTCATTCGACACAAGGTGGGTATCAATCGCCGTCGCCACCGCCTGAACGCGATGACTAAACt  <  1:1856591/69‑1 (MQ=255)
                                                       cGCTGAAGTCATTCGACACAAGGTGGGTATCAATCGCCGTCGCCACCGCCTGAACGCGATGACTAAACt  <  1:2809533/69‑1 (MQ=255)
                                                       cGCTGAAGTCATTCGACACAAGGTGGGTATCAATCGCCGTCGCCACCGCCTGAACGCGATGACTAAACt  <  1:3298625/69‑1 (MQ=255)
                                                       cGCTGAAGTCATTCGACACAAGGTGGGTATCAATCGCCGTCGCCACCGCCTGAACGCGATGACTAAACt  <  1:246302/69‑1 (MQ=255)
                                                       cGCTGAAGTCATTCGACACAAGGTGGGTATCAATCGCCGTCGCCACCGCCTGAACGCGATGACTAAACt  <  1:2557017/69‑1 (MQ=255)
                                                       cGCTGAAGTCATTCGACACAAGGTGGGTATCAATCGCCGTCGCCACCGCCTGAACGCGATGACTAAACt  <  1:2894341/69‑1 (MQ=255)
                                                       cGCTGAAGTCATTCGACACAAGGTGGGTATCAATCGCCGTCGCCACCGCCTGAACGCGATGACTAAACt  <  1:2735520/69‑1 (MQ=255)
                                                       |                                                                    
TCTACACGAACGATATCTGCCGACATCATTAATTCGGTAATTTGTGGCCTTAATACGCTGAAGTCATTCGACACAAGGTGGGTATCAATCGCCGTCGCCACCGCCTGAACGCGATGACTAAACT  >  minE/2218382‑2218505

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: