Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,219,341 T→C 37.0% A192A (GCT→GCC yhdH → predicted oxidoreductase, Zn‑dependent and NAD(P)‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,219,3410TC37.0% 27.8 / 32.1 54A192A (GCT→GCCyhdHpredicted oxidoreductase, Zn‑dependent and NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base T (16/18);  new base C (4/16);  total (20/34)
Fisher's exact test for biased strand distribution p-value = 7.89e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTATCAGGTCGTTGCCGTTTCCGGTCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCTAGCCGTGTTCTCCCTCGTGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGG  >  minE/2219277‑2219408
                                                                |                                                                   
gTTATCAGGTCGTTGCCGTTTCCGGTCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCTAGc                                                                  <  1:609989/68‑1 (MQ=255)
                 gTTTCCGGTCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCTAGCCGTGTTCTCCCTCGtgat                                                <  1:3248106/69‑1 (MQ=255)
                 gTTTCCGGTCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGCTGCTAGCCGTGTTCTCCCTCGtgat                                                <  1:388983/69‑1 (MQ=255)
                  tttCCGGTCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCTAGCCGTGTTCTCCCTCGtgatg                                               <  1:926803/69‑1 (MQ=255)
                  tttCCGGTCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCTAGCCGTGTTCTCCCTCGtgatg                                               <  1:2857552/69‑1 (MQ=255)
                  tttCCGGTCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCTAGCCGTGTTCTCCCTCGtgatg                                               <  1:1743714/69‑1 (MQ=255)
                  tttCCGGTCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCTAGCCGTGTTCTCCCTCGtgatg                                               <  1:3158370/69‑1 (MQ=255)
                  tttCCGGTCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCTAGCCGTGTTCTCCCTCGtgatg                                               <  1:708983/69‑1 (MQ=255)
                    tCCGGTCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCTAGCCGTGTTCTCCCTCGTGATGAg                                             >  1:1368292/1‑69 (MQ=255)
                    tCCGGTCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCTAGCCGTGTTCTCCCTCGTGATGAg                                             >  1:1567133/1‑69 (MQ=255)
                    tCCGGTCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCTAGCCGTGTTCTCCCTCGTGATGAg                                             <  1:386626/69‑1 (MQ=255)
                    tCCGGTCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCTAGCCGTGTTCTCCCTCGTGATGAg                                             <  1:3051063/69‑1 (MQ=255)
                    tCCGGTCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCTAGCCGTGTTCTCCCTCGTGATGAg                                             >  1:2974944/1‑69 (MQ=255)
                        gTCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCCAGCCGTATTCTTCCTCGCGATGAGttt                                          <  1:553073/68‑1 (MQ=255)
                        gTCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCCAGCCGTATTCTTCCTCGCGATGAGttt                                          <  1:2678887/68‑1 (MQ=255)
                        gTCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCCAGCCGTATTCTTCCTCGCGATGAGttt                                          <  1:476760/68‑1 (MQ=255)
                        gTCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCCAGCCGTATTCTTCCTCGCGATGAGttt                                          <  1:3210666/68‑1 (MQ=255)
                        gTCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCCAGCCGTATTCTTCCTCGCGATGAGttt                                          <  1:346057/68‑1 (MQ=255)
                        gTCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCCAGCCGTATTCTTCCTCGCGATGAGttt                                          <  1:331603/68‑1 (MQ=255)
                        gTCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCCAGCCGTATTCTTCCTCGCGATGAGttt                                          <  1:598740/68‑1 (MQ=255)
                        gTCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCCAGCCGTATTCTTCCTCGCGATGAGttt                                          <  1:26091/68‑1 (MQ=255)
                        gTCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCCAGCCGTATTCTTCCTCGCGATGAGttt                                          <  1:743995/68‑1 (MQ=255)
                        gTCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCCAGCCGTATTCTTCCTCGCGATGAGttt                                          <  1:21888/68‑1 (MQ=255)
                        gTCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCCAGCCGTATTCTTCCTCGCGATGAGttt                                          <  1:778961/68‑1 (MQ=255)
                        gTCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCCAGCCGTATTCTTCCTCGCGATGAGttt                                          <  1:892415/68‑1 (MQ=255)
                        gTCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCCAGCCGTATTCTTCCTCGCGATGAGttt                                          <  1:1489243/68‑1 (MQ=255)
                        gTCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCCAGCCGTATTCTTCCTCGCGATGAGttt                                          <  1:1059874/68‑1 (MQ=255)
                         tCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCCAGCCGTATTCTTCCTCGCGATGAGttt                                          <  1:2394115/67‑1 (MQ=255)
                         tCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCCAGCCGTATTCTTCCTCGCGATGAGttt                                          <  1:3188830/67‑1 (MQ=255)
                           gcgAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCTAGTCGTGTTCTCCCTCGTGATGAGTTTGCCg                                      <  1:2469816/69‑1 (MQ=255)
                           gcgAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCTAGCCGTGTTCTCCCTCGTGATGAGTTTGCCg                                      <  1:2734160/69‑1 (MQ=255)
                           gcgAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCTAGCCGTGTTCTCCCTCGTGATGAGTTTGCCg                                      <  1:2038914/69‑1 (MQ=255)
                           gcgAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCTAGCCGTGTTCTCCCTCGTGATGAGTTTGCCg                                      <  1:3361681/69‑1 (MQ=255)
                            cgAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCTAGCCGTGTTCTCCCTCGTGATGAGTTTGCCg                                      <  1:93875/68‑1 (MQ=255)
                             gAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCTAGCCGTGTTCTCCCTCGtgatg                                               <  1:3041168/58‑1 (MQ=255)
                                         gAATATCTGAAAAGTTTAGGTGCTAGCCGTGTTCTCCCTCGtgatg                                               <  1:2843521/46‑1 (MQ=255)
                                          aaTATCTGAAAAGTTTAGGTGCTAGCCGTGTTCTCCCTCGTGATGAGTTTGCCGAATCCCGTCCTCTg                        >  1:3197370/1‑68 (MQ=255)
                                          aaTATCTGAAAAGTTTAGGTGCTAGCCGTGTTCTCCCTCGTGATGAGTTTGCCGAATCCCGTCCTCTg                        >  1:678649/1‑68 (MQ=255)
                                                    aaGTTTAGGTGCCAGCCGTATTCTTCCTCGCGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGt             >  1:330316/1‑69 (MQ=255)
                                                    aaGTTTAGGTGCCAGCCGTATTCTTCCTCGCGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGt             >  1:3095259/1‑69 (MQ=255)
                                                    aaGTTTAGGTGCCAGCCGTATTCTTCCTCGCGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGt             >  1:892887/1‑69 (MQ=255)
                                                    aaGTTTAGGTGCCAGCCGTATTCTTCCTCGCGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGt             >  1:838736/1‑69 (MQ=255)
                                                         tAGGTGCTAGCCGTGTTCTCCCTCGTGATGAGTTTGCCGAATCCCGTcc                            >  1:278065/1‑49 (MQ=255)
                                                         tAGGTGCTAGCCGTGTTCTCCCTCGTGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTctggg        >  1:1333641/1‑69 (MQ=255)
                                                         tAGGTGCTAGCCGTGTTCTCCCTCGTGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTctggg        >  1:33074/1‑69 (MQ=255)
                                                         tAGGTGCTAGCCGTGTTCTCCCTCGTGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTctggg        >  1:1478082/1‑69 (MQ=255)
                                                         tAGGTGCTAGCCGTGTTCTCCCTCGTGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTctggg        >  1:923747/1‑69 (MQ=255)
                                                         tAGGTGCTAGCCGTGTTCTCCCTCGTGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTctggg        >  1:2078813/1‑69 (MQ=255)
                                                         tAGGTGCTAGCCGTGTTCTCCCTCGTGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTctggg        >  1:2096787/1‑69 (MQ=255)
                                                         tAGGTGCTAGCCGTGTTCTCCCTCGTGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTctggg        >  1:2388202/1‑69 (MQ=255)
                                                         tAGGTGCTAGCCGTGTTCTCCCTCGTGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTctggg        >  1:2653636/1‑69 (MQ=255)
                                                         tAGGTGCTAGCCGTGTTCTCCCTCGTGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTctggg        >  1:3401955/1‑69 (MQ=255)
                                                              gCTAGCCGTGTTCTCCCTCGTGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTctgggctgg    >  1:477093/1‑68 (MQ=255)
                                                               cTAGCCGTGTTCTCCCTCGTGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTgggg  <  1:2396023/69‑1 (MQ=255)
                                                                |                                                                   
GTTATCAGGTCGTTGCCGTTTCCGGTCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCTAGCCGTGTTCTCCCTCGTGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGG  >  minE/2219277‑2219408

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: