| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,219,341 | T→C | 37.0% | A192A (GCT→GCC) | yhdH → | predicted oxidoreductase, Zn‑dependent and NAD(P)‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,219,341 | 0 | T | C | 37.0% | 27.8 / 32.1 | 54 | A192A (GCT→GCC) | yhdH | predicted oxidoreductase, Zn‑dependent and NAD(P)‑binding |
| Reads supporting (aligned to +/- strand): ref base T (16/18); new base C (4/16); total (20/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.89e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTTATCAGGTCGTTGCCGTTTCCGGTCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCTAGCCGTGTTCTCCCTCGTGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGG > minE/2219277‑2219408 | gTTATCAGGTCGTTGCCGTTTCCGGTCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCTAGc < 1:609989/68‑1 (MQ=255) gTTTCCGGTCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCTAGCCGTGTTCTCCCTCGtgat < 1:3248106/69‑1 (MQ=255) gTTTCCGGTCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGCTGCTAGCCGTGTTCTCCCTCGtgat < 1:388983/69‑1 (MQ=255) tttCCGGTCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCTAGCCGTGTTCTCCCTCGtgatg < 1:926803/69‑1 (MQ=255) tttCCGGTCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCTAGCCGTGTTCTCCCTCGtgatg < 1:2857552/69‑1 (MQ=255) tttCCGGTCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCTAGCCGTGTTCTCCCTCGtgatg < 1:1743714/69‑1 (MQ=255) tttCCGGTCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCTAGCCGTGTTCTCCCTCGtgatg < 1:3158370/69‑1 (MQ=255) tttCCGGTCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCTAGCCGTGTTCTCCCTCGtgatg < 1:708983/69‑1 (MQ=255) tCCGGTCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCTAGCCGTGTTCTCCCTCGTGATGAg > 1:1368292/1‑69 (MQ=255) tCCGGTCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCTAGCCGTGTTCTCCCTCGTGATGAg > 1:1567133/1‑69 (MQ=255) tCCGGTCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCTAGCCGTGTTCTCCCTCGTGATGAg < 1:386626/69‑1 (MQ=255) tCCGGTCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCTAGCCGTGTTCTCCCTCGTGATGAg < 1:3051063/69‑1 (MQ=255) tCCGGTCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCTAGCCGTGTTCTCCCTCGTGATGAg > 1:2974944/1‑69 (MQ=255) gTCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCCAGCCGTATTCTTCCTCGCGATGAGttt < 1:553073/68‑1 (MQ=255) gTCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCCAGCCGTATTCTTCCTCGCGATGAGttt < 1:2678887/68‑1 (MQ=255) gTCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCCAGCCGTATTCTTCCTCGCGATGAGttt < 1:476760/68‑1 (MQ=255) gTCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCCAGCCGTATTCTTCCTCGCGATGAGttt < 1:3210666/68‑1 (MQ=255) gTCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCCAGCCGTATTCTTCCTCGCGATGAGttt < 1:346057/68‑1 (MQ=255) gTCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCCAGCCGTATTCTTCCTCGCGATGAGttt < 1:331603/68‑1 (MQ=255) gTCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCCAGCCGTATTCTTCCTCGCGATGAGttt < 1:598740/68‑1 (MQ=255) gTCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCCAGCCGTATTCTTCCTCGCGATGAGttt < 1:26091/68‑1 (MQ=255) gTCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCCAGCCGTATTCTTCCTCGCGATGAGttt < 1:743995/68‑1 (MQ=255) gTCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCCAGCCGTATTCTTCCTCGCGATGAGttt < 1:21888/68‑1 (MQ=255) gTCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCCAGCCGTATTCTTCCTCGCGATGAGttt < 1:778961/68‑1 (MQ=255) gTCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCCAGCCGTATTCTTCCTCGCGATGAGttt < 1:892415/68‑1 (MQ=255) gTCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCCAGCCGTATTCTTCCTCGCGATGAGttt < 1:1489243/68‑1 (MQ=255) gTCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCCAGCCGTATTCTTCCTCGCGATGAGttt < 1:1059874/68‑1 (MQ=255) tCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCCAGCCGTATTCTTCCTCGCGATGAGttt < 1:2394115/67‑1 (MQ=255) tCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCCAGCCGTATTCTTCCTCGCGATGAGttt < 1:3188830/67‑1 (MQ=255) gcgAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCTAGTCGTGTTCTCCCTCGTGATGAGTTTGCCg < 1:2469816/69‑1 (MQ=255) gcgAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCTAGCCGTGTTCTCCCTCGTGATGAGTTTGCCg < 1:2734160/69‑1 (MQ=255) gcgAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCTAGCCGTGTTCTCCCTCGTGATGAGTTTGCCg < 1:2038914/69‑1 (MQ=255) gcgAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCTAGCCGTGTTCTCCCTCGTGATGAGTTTGCCg < 1:3361681/69‑1 (MQ=255) cgAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCTAGCCGTGTTCTCCCTCGTGATGAGTTTGCCg < 1:93875/68‑1 (MQ=255) gAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCTAGCCGTGTTCTCCCTCGtgatg < 1:3041168/58‑1 (MQ=255) gAATATCTGAAAAGTTTAGGTGCTAGCCGTGTTCTCCCTCGtgatg < 1:2843521/46‑1 (MQ=255) aaTATCTGAAAAGTTTAGGTGCTAGCCGTGTTCTCCCTCGTGATGAGTTTGCCGAATCCCGTCCTCTg > 1:3197370/1‑68 (MQ=255) aaTATCTGAAAAGTTTAGGTGCTAGCCGTGTTCTCCCTCGTGATGAGTTTGCCGAATCCCGTCCTCTg > 1:678649/1‑68 (MQ=255) aaGTTTAGGTGCCAGCCGTATTCTTCCTCGCGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGt > 1:330316/1‑69 (MQ=255) aaGTTTAGGTGCCAGCCGTATTCTTCCTCGCGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGt > 1:3095259/1‑69 (MQ=255) aaGTTTAGGTGCCAGCCGTATTCTTCCTCGCGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGt > 1:892887/1‑69 (MQ=255) aaGTTTAGGTGCCAGCCGTATTCTTCCTCGCGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGt > 1:838736/1‑69 (MQ=255) tAGGTGCTAGCCGTGTTCTCCCTCGTGATGAGTTTGCCGAATCCCGTcc > 1:278065/1‑49 (MQ=255) tAGGTGCTAGCCGTGTTCTCCCTCGTGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTctggg > 1:1333641/1‑69 (MQ=255) tAGGTGCTAGCCGTGTTCTCCCTCGTGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTctggg > 1:33074/1‑69 (MQ=255) tAGGTGCTAGCCGTGTTCTCCCTCGTGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTctggg > 1:1478082/1‑69 (MQ=255) tAGGTGCTAGCCGTGTTCTCCCTCGTGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTctggg > 1:923747/1‑69 (MQ=255) tAGGTGCTAGCCGTGTTCTCCCTCGTGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTctggg > 1:2078813/1‑69 (MQ=255) tAGGTGCTAGCCGTGTTCTCCCTCGTGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTctggg > 1:2096787/1‑69 (MQ=255) tAGGTGCTAGCCGTGTTCTCCCTCGTGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTctggg > 1:2388202/1‑69 (MQ=255) tAGGTGCTAGCCGTGTTCTCCCTCGTGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTctggg > 1:2653636/1‑69 (MQ=255) tAGGTGCTAGCCGTGTTCTCCCTCGTGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTctggg > 1:3401955/1‑69 (MQ=255) gCTAGCCGTGTTCTCCCTCGTGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTctgggctgg > 1:477093/1‑68 (MQ=255) cTAGCCGTGTTCTCCCTCGTGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTgggg < 1:2396023/69‑1 (MQ=255) | GTTATCAGGTCGTTGCCGTTTCCGGTCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCTAGCCGTGTTCTCCCTCGTGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGG > minE/2219277‑2219408 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |