| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,219,359 | T→C | 41.6% | R198R (CGT→CGC) | yhdH → | predicted oxidoreductase, Zn‑dependent and NAD(P)‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,219,359 | 0 | T | C | 41.6% | 18.0 / 42.6 | 60 | R198R (CGT→CGC) | yhdH | predicted oxidoreductase, Zn‑dependent and NAD(P)‑binding |
| Reads supporting (aligned to +/- strand): ref base T (17/18); new base C (4/21); total (21/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.32e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
GTTTCCGGTCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCTAGCCGTGTTCTCCCTCGTGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTG > minE/2219294‑2219423 | gTTTCCGGTCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCTAGCCGTGTTCTCCCTCGtgat < 1:3248106/69‑1 (MQ=255)gTTTCCGGTCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGCTGCTAGCCGTGTTCTCCCTCGtgat < 1:388983/69‑1 (MQ=255) tttCCGGTCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCTAGCCGTGTTCTCCCTCGtgatg < 1:1743714/69‑1 (MQ=255) tttCCGGTCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCTAGCCGTGTTCTCCCTCGtgatg < 1:2857552/69‑1 (MQ=255) tttCCGGTCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCTAGCCGTGTTCTCCCTCGtgatg < 1:3158370/69‑1 (MQ=255) tttCCGGTCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCTAGCCGTGTTCTCCCTCGtgatg < 1:708983/69‑1 (MQ=255) tttCCGGTCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCTAGCCGTGTTCTCCCTCGtgatg < 1:926803/69‑1 (MQ=255) tCCGGTCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCTAGCCGTGTTCTCCCTCGTGATGAg > 1:2974944/1‑69 (MQ=255) tCCGGTCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCTAGCCGTGTTCTCCCTCGTGATGAg > 1:1567133/1‑69 (MQ=255) tCCGGTCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCTAGCCGTGTTCTCCCTCGTGATGAg < 1:3051063/69‑1 (MQ=255) tCCGGTCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCTAGCCGTGTTCTCCCTCGTGATGAg < 1:386626/69‑1 (MQ=255) tCCGGTCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCTAGCCGTGTTCTCCCTCGTGATGAg > 1:1368292/1‑69 (MQ=255) gTCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCCAGCCGTATTCTTCCTCGCGATGAGttt < 1:3210666/68‑1 (MQ=255) gTCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCCAGCCGTATTCTTCCTCGCGATGAGttt < 1:476760/68‑1 (MQ=255) gTCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCCAGCCGTATTCTTCCTCGCGATGAGttt < 1:2678887/68‑1 (MQ=255) gTCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCCAGCCGTATTCTTCCTCGCGATGAGttt < 1:598740/68‑1 (MQ=255) gTCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCCAGCCGTATTCTTCCTCGCGATGAGttt < 1:26091/68‑1 (MQ=255) gTCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCCAGCCGTATTCTTCCTCGCGATGAGttt < 1:1059874/68‑1 (MQ=255) gTCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCCAGCCGTATTCTTCCTCGCGATGAGttt < 1:21888/68‑1 (MQ=255) gTCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCCAGCCGTATTCTTCCTCGCGATGAGttt < 1:743995/68‑1 (MQ=255) gTCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCCAGCCGTATTCTTCCTCGCGATGAGttt < 1:778961/68‑1 (MQ=255) gTCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCCAGCCGTATTCTTCCTCGCGATGAGttt < 1:892415/68‑1 (MQ=255) gTCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCCAGCCGTATTCTTCCTCGCGATGAGttt < 1:553073/68‑1 (MQ=255) gTCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCCAGCCGTATTCTTCCTCGCGATGAGttt < 1:331603/68‑1 (MQ=255) gTCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCCAGCCGTATTCTTCCTCGCGATGAGttt < 1:1489243/68‑1 (MQ=255) gTCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCCAGCCGTATTCTTCCTCGCGATGAGttt < 1:346057/68‑1 (MQ=255) tCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCCAGCCGTATTCTTCCTCGCGATGAGttt < 1:3188830/67‑1 (MQ=255) tCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCCAGCCGTATTCTTCCTCGCGATGAGttt < 1:2394115/67‑1 (MQ=255) gcgAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCTAGTCGTGTTCTCCCTCGTGATGAGTTTGCCg < 1:2469816/69‑1 (MQ=255) gcgAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCTAGCCGTGTTCTCCCTCGTGATGAGTTTGCCg < 1:2038914/69‑1 (MQ=255) gcgAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCTAGCCGTGTTCTCCCTCGTGATGAGTTTGCCg < 1:2734160/69‑1 (MQ=255) gcgAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCTAGCCGTGTTCTCCCTCGTGATGAGTTTGCCg < 1:3361681/69‑1 (MQ=255) cgAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCTAGCCGTGTTCTCCCTCGTGATGAGTTTGCCg < 1:93875/68‑1 (MQ=255) gAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCTAGCCGTGTTCTCCCTCGtgatg < 1:3041168/58‑1 (MQ=255) gAATATCTGAAAAGTTTAGGTGCTAGCCGTGTTCTCCCTCGtgatg < 1:2843521/46‑1 (MQ=255) aaTATCTGAAAAGTTTAGGTGCTAGCCGTGTTCTCCCTCGTGATGAGTTTGCCGAATCCCGTCCTCTg > 1:678649/1‑68 (MQ=255) aaTATCTGAAAAGTTTAGGTGCTAGCCGTGTTCTCCCTCGTGATGAGTTTGCCGAATCCCGTCCTCTg > 1:3197370/1‑68 (MQ=255) aaGTTTAGGTGCCAGCCGTATTCTTCCTCGCGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGt > 1:892887/1‑69 (MQ=255) aaGTTTAGGTGCCAGCCGTATTCTTCCTCGCGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGt > 1:330316/1‑69 (MQ=255) aaGTTTAGGTGCCAGCCGTATTCTTCCTCGCGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGt > 1:3095259/1‑69 (MQ=255) aaGTTTAGGTGCCAGCCGTATTCTTCCTCGCGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGt > 1:838736/1‑69 (MQ=255) tAGGTGCTAGCCGTGTTCTCCCTCGTGATGAGTTTGCCGAATCCCGTcc > 1:278065/1‑49 (MQ=255) tAGGTGCTAGCCGTGTTCTCCCTCGTGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTctggg > 1:2096787/1‑69 (MQ=255) tAGGTGCTAGCCGTGTTCTCCCTCGTGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTctggg > 1:2078813/1‑69 (MQ=255) tAGGTGCTAGCCGTGTTCTCCCTCGTGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTctggg > 1:2388202/1‑69 (MQ=255) tAGGTGCTAGCCGTGTTCTCCCTCGTGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTctggg > 1:33074/1‑69 (MQ=255) tAGGTGCTAGCCGTGTTCTCCCTCGTGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTctggg > 1:2653636/1‑69 (MQ=255) tAGGTGCTAGCCGTGTTCTCCCTCGTGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTctggg > 1:1478082/1‑69 (MQ=255) tAGGTGCTAGCCGTGTTCTCCCTCGTGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTctggg > 1:3401955/1‑69 (MQ=255) tAGGTGCTAGCCGTGTTCTCCCTCGTGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTctggg > 1:1333641/1‑69 (MQ=255) tAGGTGCTAGCCGTGTTCTCCCTCGTGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTctggg > 1:923747/1‑69 (MQ=255) gCTAGCCGTGTTCTCCCTCGTGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTctgggctgg > 1:477093/1‑68 (MQ=255) cTAGCCGTGTTCTCCCTCGTGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTgggg < 1:2396023/69‑1 (MQ=255) aGCCGTGTTCTCCCTCGTGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGc < 1:1531192/68‑1 (MQ=255) aGCCGTGTTCTCCCTCGTGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGc < 1:897812/68‑1 (MQ=255) aGCCGTGTTCTCCCTCGTGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCa < 1:2994910/69‑1 (MQ=255) aGCCGTGTTCTCCCTCGTGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCa < 1:474111/69‑1 (MQ=255) aGCCGTGTTCTCCCTCGTGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCa < 1:2236750/69‑1 (MQ=255) ttCTCCCTCGTGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGaca > 1:1445698/1‑69 (MQ=255) ctcCCTCGTGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACAcc < 1:105281/69‑1 (MQ=255) tCGCGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTg < 1:3005007/68‑1 (MQ=255) tCGCGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTg < 1:202496/68‑1 (MQ=255) tCGCGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTg < 1:1689733/68‑1 (MQ=255) tCGCGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTg < 1:1655965/68‑1 (MQ=255) tCGCGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTg < 1:1229656/68‑1 (MQ=255) gTGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAAtt < 1:331462/56‑1 (MQ=255) gTGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAAtt < 1:1000070/56‑1 (MQ=255) gTGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAAtt < 1:1570023/56‑1 (MQ=255) gTGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAAtt < 1:1082271/56‑1 (MQ=255) tgatgaGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAAtt < 1:1575943/55‑1 (MQ=255) | GTTTCCGGTCGCGAAAGTACCCATGAATATCTGAAAAGTTTAGGTGCTAGCCGTGTTCTCCCTCGTGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTG > minE/2219294‑2219423 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |