| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,221,351 | T→C | 34.7% | I51I (ATT→ATC) | accC → | acetyl‑CoA carboxylase, biotin carboxylase subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,221,351 | 0 | T | C | 34.7% | 60.6 / 51.5 | 90 | I51I (ATT→ATC) | accC | acetyl‑CoA carboxylase, biotin carboxylase subunit |
| Reads supporting (aligned to +/- strand): ref base T (31/27); new base C (18/13); total (50/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.23e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.41e-01 | |||||||||||
TCGCTGTGCACTCCAGCGCGGATCGCGATCTAAAACACGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCG > minE/2221284‑2221418 | tCGCTGTGCACTCCAGCGCGGATCGCTATCTAAAACACGTATTACTGGCAGATGAAACGGTCTGTATTg > 1:1677118/1‑69 (MQ=255)tCGCTGTGCACTCCAGCGCGGATCGCGATCTAAAACACGTATTACTGGCAGATGAAACGGTCTGTATTg > 1:2934874/1‑69 (MQ=255)tCGCTGTGCACTCCAGCGCGGATCGCGATCTAAAACACGTATTACTGGCAGATGAAACGGTCTGTATTg > 1:2296701/1‑69 (MQ=255)tCGCTGTGCACTCCAGCGCGGATCGCGATCTAAAACACGTATTACTGGCAGATGAAACGGTCTGTATTg > 1:2307242/1‑69 (MQ=255)tCGCTGTGCACTCCAGCGCGGATCGCGATCTAAAACACGTATTACTGGCAGATGAAACGGTCTGTATTg > 1:317181/1‑69 (MQ=255)tCGCTGTGCACTCCAGCGCGGATCGCGATCTAAAACACGTATTACTGGCAGATGAAACGGTCTGTATTg > 1:3434387/1‑69 (MQ=255)tCGCTGTGCACTCCAGCGCGGATCGCGATCTAAAACACGTATTACTGGCAGATGAAACGGTCTGTATTg > 1:559253/1‑69 (MQ=255)tCGCTGTGCACTCCAGCGCGGATCGCGATCTAAAACACGTATTACTGGCAGATGAAACGGTCTGTATTg > 1:1183261/1‑69 (MQ=255)tCGCTGTGCACTCCAGCGCGGATCGCGATCTAAAACACGTATTACTGGCAGATGAAACGGTCTGTATTg > 1:1161475/1‑69 (MQ=255)tCGCTGTGCACTCCAGCGCGGATCGCGATCTAAAACACGTATTACTGGCAGATGAAACGGTCTGTATTg > 1:1150632/1‑69 (MQ=255)tCGCTGTGCACTCCAGCGCGGATCGAGATCTAAAACACGTATTACTGGCAGATGAAACGGTCTGTATTg > 1:2339418/1‑69 (MQ=255) gCTGTGCACTCCAGCGCGGATCGCGATCTAAAACACGTATTACTGGCAGATGAAACGGTCTGTATTgg > 1:848267/1‑68 (MQ=255) gCTGTGCACTCCAGCGCGGATCGCGATCTAAAACACGTATTACTGGCAGATGAAACGGTCTGTATTGGc > 1:677439/1‑69 (MQ=255) gCTGTGCACTCCAGCGCGGATCGCGATCTAAAACACGTATTACTGGCAGATGAAACGGTCTGTATTGGc > 1:2829975/1‑69 (MQ=255) gCTGTGCACTCCAGCGCGGATCGCGATCTAAAACACGTATTACTGGCAGATGAAACGGTCTGTATTGGc > 1:2796250/1‑69 (MQ=255) gCTGTGCACTCCAGCGCGGATCGCGATCTAAAACACGTATTACTGGCAGATGAAACGGTCTGTATTGGc > 1:2677960/1‑69 (MQ=255) gtgCACTCCAGCGCGGATCGCGATCTAAAACACGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCt > 1:1774612/1‑69 (MQ=255) cACTCCAGCGCGGATCGCGATCTAAAACACGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGc < 1:2569333/68‑1 (MQ=255) cACTCCAGCGCGGATCGCGATCTAAAACACGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCt > 1:64171/1‑69 (MQ=255) cACTCCAGCGCGGATCGCGATCTAAAACACGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCt > 1:2599368/1‑69 (MQ=255) cACTCCAGCGCGGATCGCGATCTAAAACACGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCt > 1:1959913/1‑69 (MQ=255) ccAGCGCGGATCGCGATCTAAAACACGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCTcc > 1:2918615/1‑67 (MQ=255) ccAGCGCGGATCGCGATCTAAAACACGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCTcc > 1:1992966/1‑67 (MQ=255) ccAGCGCGGATCGCGATCTAAAACACGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCTcc > 1:985686/1‑67 (MQ=255) ccAGCGCGGATCGCGATCTAAAACACGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCTcc > 1:926045/1‑67 (MQ=255) ccAGCGCGGATCGCGATCTAAAACACGTATTACTGGCAGATGAAACGGTCTGTATGGGCCCTGCTcc > 1:1964088/1‑67 (MQ=255) cgcgATCTAAAACACGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCTCCGTCAGTAAAAAGt > 1:1631571/1‑69 (MQ=255) cgcgATCTAAAACACGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCTCCGTCAGTAAAAAGt > 1:1543346/1‑69 (MQ=255) cgcgATCTAAAACACGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCTCCGTCAGTAAAAAGt > 1:1183522/1‑69 (MQ=255) cgcgATCTAAAACACGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCTCCGTCAGTAAAAAGt > 1:1652144/1‑69 (MQ=255) cgcgATCTAAAACACGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCTCCGTCAGTAAAAAGt > 1:1823228/1‑69 (MQ=255) cTAAAACACGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCTCCGTCAGTAAAAAGTTATCTg > 1:961824/1‑69 (MQ=255) acacGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCTCCGTCAGTAAAAAGTTATCTg < 1:1287485/64‑1 (MQ=255) acacGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCTCCGTCAGTAAAAAGTTATCTg < 1:2349214/64‑1 (MQ=255) acacGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCTCCGTCAGTAAAAAGTTATCTg < 1:888990/64‑1 (MQ=255) acacGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCTCCGTCAGTAAAAAGTTATCTg < 1:1648692/64‑1 (MQ=255) acacGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCTCCGTCAGTAAAAAGTTATCTg < 1:1945831/64‑1 (MQ=255) acacGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCTCCGTCAGTAAAAAGTTATCTg < 1:1926623/64‑1 (MQ=255) acacGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCTCCGTCAGTAAAAAGTTATCTg < 1:296425/64‑1 (MQ=255) acacGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCTCCGTCAGTAAAAAGTTATCTg < 1:168336/64‑1 (MQ=255) acacGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCTCCGTCAGTAAAAAGTTATCTGa < 1:626586/65‑1 (MQ=255) acacGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCTCCGTCAGTAAAAAGTTATCTGa < 1:180761/65‑1 (MQ=255) acacGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCTCCGTCAGTAAAAAGTTATCTGa < 1:2067206/65‑1 (MQ=255) acacGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCTCCGTCAGTAAAAAGTTATCTGa < 1:1756275/65‑1 (MQ=255) acacGTATTACTGGCAGATCAAACGGTCTGTATTGGCCCTGCTCCGTCAGTAAAAAGTTATCTGa < 1:1733854/65‑1 (MQ=255) acacGTATTACTCGCAGATGAAACGGTCTGTATTGGCCCTGCTCCGTCAGTAAAAAGTTATCTg < 1:2863348/64‑1 (MQ=255) cacGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCTCCGTCAGTAAAAAGTTATCTg < 1:3065405/63‑1 (MQ=255) cacGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCTCCGTCAGTAAAAAGTTATCTg < 1:124592/63‑1 (MQ=255) cacGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCTCCGTCAGTAAAAAGTTATCTg < 1:1143549/63‑1 (MQ=255) aCTGGCAGATGAAACGGTCTGTATTGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCaa > 1:1437097/1‑68 (MQ=255) aGATGAAACGGTCTGTATCGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAatcatc < 1:1454898/67‑1 (MQ=255) aGATGAAACGGTCTGTATCGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAatcatc < 1:1887488/67‑1 (MQ=255) aGATGAAACGGTCTGTATCGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAatcatc < 1:2932848/67‑1 (MQ=255) aGATGAAACGGTCTGTATCGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAatcatc < 1:526114/67‑1 (MQ=255) aGATGAAACGGTCTGTATCGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAatcatc < 1:3071528/67‑1 (MQ=255) aGATGAAACGGTCTGTATCGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAatcatc < 1:1634728/67‑1 (MQ=255) aGATGAAACGGTCTGTATCGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAatcatc < 1:2667253/67‑1 (MQ=255) aGATGAAACGGTCTGTATCGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAatcatc < 1:354507/67‑1 (MQ=255) aGATGAAACGGTCTGTATCGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAatcatc < 1:728403/67‑1 (MQ=255) aGATGAAACGGTCTGTATCGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAatcatc < 1:2519953/67‑1 (MQ=255) aGATGAAACGGTCTGTATCGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAatcatc < 1:1582556/67‑1 (MQ=255) aGATGAAACGGTCTGTATCGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAatcatc < 1:2383964/67‑1 (MQ=255) aGATGAAACGGTCTGTATCGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAatcatc < 1:777585/67‑1 (MQ=255) aTGAAACGGTCTGTATCTGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAatca > 1:622368/1‑63 (MQ=255) aTGAAACGGTCTGTATCGGCCCTGCTCCGTCAGTAAAAAGTTAt > 1:8090/1‑44 (MQ=255) aTGAAACGGTCTGTATCGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCaa > 1:2905250/1‑60 (MQ=255) aTGAAACGGTCTGTATCGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAatca > 1:1142388/1‑63 (MQ=255) aTGAAACGGTCTGTATCGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAatca > 1:1680643/1‑63 (MQ=255) aTGAAACGGTCTGTATCGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAatca > 1:927342/1‑63 (MQ=255) aTGAAACGGTCTGTATCGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAatca > 1:513063/1‑63 (MQ=255) aTGAAACGGTCTGTATCGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAatca > 1:1223614/1‑63 (MQ=255) aTGAAACGGTCTGTATCGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAatca > 1:1207708/1‑63 (MQ=255) aTGAAACGGTCTGTATCGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAatca > 1:726532/1‑63 (MQ=255) aTGAAACGGTCTGTATCGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAatca > 1:736123/1‑63 (MQ=255) aTGAAACGGTCTGTATCGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAatca > 1:1824975/1‑63 (MQ=255) aTGAAACGGTCTGTATCGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAatca > 1:1868101/1‑63 (MQ=255) aTGAAACGGTCTGTATCGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAatca > 1:2728832/1‑63 (MQ=255) aTGAAACGGTCTGTATCGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAatca > 1:2049337/1‑63 (MQ=255) aTGAAACGGTCTGTATCGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAatca > 1:2163104/1‑63 (MQ=255) aTGAAACGGTCTGTATCGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAatca > 1:150211/1‑63 (MQ=255) aTGAAACGGTCTGTATCGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAatca > 1:2456771/1‑63 (MQ=255) ggTCTGTATTGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGa < 1:1449222/69‑1 (MQ=255) ggTCTGTATTGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGa < 1:908488/69‑1 (MQ=255) ggTCTGTATTGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGa < 1:2676094/69‑1 (MQ=255) ggTCTGTATTGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGa < 1:2180120/69‑1 (MQ=255) gTCTGTATTGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGaa < 1:1660224/69‑1 (MQ=255) gTCTGTATTGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGaa < 1:3270057/69‑1 (MQ=255) gTCTGTATTGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGaa < 1:2650231/69‑1 (MQ=255) gTCTGTATTGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGaa < 1:138097/69‑1 (MQ=255) tCTGTATTGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGaa < 1:2742084/68‑1 (MQ=255) ttGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCAcc < 1:162956/68‑1 (MQ=255) ttGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCAcc > 1:1322940/1‑68 (MQ=255) ttGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCg > 1:709636/1‑69 (MQ=255) ttGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCg > 1:1197084/1‑69 (MQ=255) ttGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCg > 1:1925593/1‑69 (MQ=255) ttGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCg > 1:55167/1‑69 (MQ=255) ttGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCg > 1:940701/1‑69 (MQ=255) | TCGCTGTGCACTCCAGCGCGGATCGCGATCTAAAACACGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCG > minE/2221284‑2221418 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |