| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,222,098 | A→T | 20.3% | T300T (ACA→ACT) | accC → | acetyl‑CoA carboxylase, biotin carboxylase subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,222,098 | 0 | A | T | 20.3% | 105.9 / 21.1 | 59 | T300T (ACA→ACT) | accC | acetyl‑CoA carboxylase, biotin carboxylase subunit |
| Reads supporting (aligned to +/- strand): ref base A (22/25); new base T (7/5); total (29/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.32e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.71e-01 | |||||||||||
GTTCGAAAACGGCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATCGCTGCCGGTCAACCGCTGTC > minE/2222032‑2222163 | gTTCGAAAACGGCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACAGa < 1:1126292/69‑1 (MQ=255)gTTCGAAAACGGCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACAGa < 1:495871/69‑1 (MQ=255)gTTCGAAAACGGCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACAGa < 1:3315824/69‑1 (MQ=255)gTTCGAAAACGGCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACAGa < 1:2542474/69‑1 (MQ=255)gTTCGAAAACGGCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACAGa < 1:2499430/69‑1 (MQ=255)gTTCGAAAACGGCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACAGa < 1:1392706/69‑1 (MQ=255)gTTCGAAAACGGCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACAGa < 1:2341364/69‑1 (MQ=255)gTTCGAAAACGGCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACAGa < 1:2220227/69‑1 (MQ=255)gTTCGAAAACGGCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACAGa < 1:2012703/69‑1 (MQ=255) ttCGAAAACGGCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACAGa < 1:98797/68‑1 (MQ=255) aaaaCGGCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACAGAAATg < 1:1630676/68‑1 (MQ=255) aaaaCGGCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACAGAAATg < 1:1676233/68‑1 (MQ=255) aaaaCGGCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACAGAAATg < 1:965375/68‑1 (MQ=255) aaaaCGGCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACAGAAATg < 1:909010/68‑1 (MQ=255) aaaaCGGCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACAGAAATg < 1:2695945/68‑1 (MQ=255) aaaaCGGCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACAGAAATg < 1:1877511/68‑1 (MQ=255) aaaaCGGCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACAGAAATg < 1:2999803/68‑1 (MQ=255) aaaaCGGCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACAGAAATg < 1:3075110/68‑1 (MQ=255) aaaaCGGCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACAGAAATg < 1:397023/68‑1 (MQ=255) aCGGCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACAGAAATg > 1:986480/1‑65 (MQ=255) aCGGCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACAGAAATg > 1:3134974/1‑65 (MQ=255) cgtgTTCTATTTCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACAGAAATg < 1:3426152/58‑1 (MQ=255) ttCTATTTCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGtt > 1:462951/1‑69 (MQ=255) ttCTATTTCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGtt > 1:884456/1‑69 (MQ=255) tCTATTTCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTg > 1:3410777/1‑69 (MQ=255) cTATTTCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACAGAAATg > 1:668650/1‑55 (MQ=255) cTATTTCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACAGAAATg > 1:85360/1‑55 (MQ=255) cTATTTCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACAGAAATg > 1:875355/1‑55 (MQ=255) cTATTTCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGa > 1:1543685/1‑69 (MQ=255) cTATTTCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGa > 1:2292664/1‑69 (MQ=255) tCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACTGAAATGATCACCGGCGTTGAc < 1:1593493/65‑1 (MQ=255) tCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACTGAAATGATCACCGGCGTTGAc < 1:2628246/65‑1 (MQ=255) tCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACTGAAATGATCACCGGCGTTGAc < 1:1218052/65‑1 (MQ=255) tCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACTGAAATGATCACCGGCGTTGAc < 1:1544815/65‑1 (MQ=255) tCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACTGAAATGATCACCGGCGTTGAc < 1:1479699/65‑1 (MQ=255) aaaTGAACACCCGTATTCAGGTAGAACACCCGGTTACAGAAATGATCAcc < 1:619525/50‑1 (MQ=255) ttCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATCg > 1:1806315/1‑69 (MQ=255) ttCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATCg > 1:2144010/1‑69 (MQ=255) ttCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATCg > 1:215931/1‑69 (MQ=255) aGAACACCCGGTTACTGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATCGCTGc > 1:403797/1‑66 (MQ=255) aGAACACCCGGTTACTGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATCGCTGc > 1:1580953/1‑66 (MQ=255) aGAACACCCGGTTACTGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATCGCTGc > 1:1763119/1‑66 (MQ=255) aGAACACCCGGTTACTGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATCGCTGc > 1:624258/1‑66 (MQ=255) aGAACACCCGGTTACTGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATCGCTGc > 1:2160413/1‑66 (MQ=255) aGAACACCCGGTTACTGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATCGCTGc > 1:2275258/1‑66 (MQ=255) aGAACACCCGGTTACTGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATCGCTGc > 1:2283724/1‑66 (MQ=255) acCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATCGCTGCCGGTCAAc < 1:3359791/69‑1 (MQ=255) acCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATCGCTGCCGGTCAAc < 1:113156/69‑1 (MQ=255) acCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATCGCTGCCGGTCAAc < 1:8640/69‑1 (MQ=255) acCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATCGCTGCCGGTCAAc < 1:2761091/69‑1 (MQ=255) tACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATCGCTGCCGGTCAACCGCTGTc > 1:2578053/1‑69 (MQ=255) tACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATCGCTGCCGGTCAACCGCTGTc > 1:1175655/1‑69 (MQ=255) tACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATCGCTGCCGGTCAACCGCTGTc > 1:2081371/1‑69 (MQ=255) tACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATCGCTGCCGGTCAACCGCTGTc > 1:2420004/1‑69 (MQ=255) tACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATCGCTGCCGGTCAACCGCTGTc > 1:1885500/1‑69 (MQ=255) tACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATCGCTGCCGGTCAACCGCTGTc > 1:2730798/1‑69 (MQ=255) tACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATCGCTGCCGGTCAACCGCTGTc > 1:173666/1‑69 (MQ=255) tACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATCGCTGCCGGTCAACCGCTGTc > 1:1296764/1‑69 (MQ=255) tACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATCGCTGCCGGTCAACCGCTGTc > 1:1123217/1‑69 (MQ=255) | GTTCGAAAACGGCGAGTTCTATTTCATCGAAATGAACACCCGTATTCAGGTAGAACACCCGGTTACAGAAATGATCACCGGCGTTGACCTGATCAAAGAACAGCTGCGTATCGCTGCCGGTCAACCGCTGTC > minE/2222032‑2222163 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |