| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,223,383 | T→C | 35.1% | F165F (TTT→TTC) | panF → | pantothenate:sodium symporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,223,383 | 0 | T | C | 35.1% | 32.5 / 29.5 | 54 | F165F (TTT→TTC) | panF | pantothenate:sodium symporter |
| Reads supporting (aligned to +/- strand): ref base T (15/20); new base C (8/11); total (23/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTTATCGGCGGTGCGCGCCTGCTGGAAACCGCGGCGGGTATTCCTTATGAAACCGGGCTGCTGATTTTTGGTATCAGCATTGCGTTATATACCGCCTTTGGTGGCTTTCGCGCCAGCGTGCTGAAC > minE/2223315‑2223440 | tttATCGGCGGTGCGCGCCTGCTGGAAACCGCGGCGGGTATTCCTTATGAAACCGGGCTGCTGAttttt > 1:2640657/1‑69 (MQ=255) tATCGGCGGTGCGCGCCTGCTGGAAACCGCGGCGGGTATTCCTTATGAAACCGGGCTGCTGATTTTTgg > 1:83460/1‑69 (MQ=255) tATCGGCGGTGCGCGCCTGCTGGAAACCGCGGCGGGTATTCCTTATGAAACCGGGCTGCTGATTTTTgg > 1:2007219/1‑69 (MQ=255) tATCGGCGGTGCGCGCCTGCTGGAAACCGCGGCGGGTATTCCTTATGAAACCGGGCTGCTGATTTTTgg > 1:2668449/1‑69 (MQ=255) tATCGGCGGTGCGCGCCTGCTGGAAACCGCGGCGGGTATTCCTTATGAAACCGGGCTGCTGATTTTTgg > 1:1833303/1‑69 (MQ=255) tATCGGCGGTGCGCGCCTGCTGGAAACCGCGGCGGGTATTCCTTATGAAACCGGGCTGCTGATTTTTgg > 1:3148200/1‑69 (MQ=255) tATCGGCGGTGCGCGCCTGCTGGAAACCGCGGCGGGTATTCCTTATGAAACCGGGCTGCTGATTTTTgg > 1:1351253/1‑69 (MQ=255) tATCGGCGGTGCGCGCCTGCTGGAAACCGCGGCGGGTATTCCTTATGAAACCGGGCTGCTGATTTTTgg > 1:767905/1‑69 (MQ=255) ggTGCGCGCCTGCTGGAAACCGCGGCGGGTATTCCTTCTGAAACCGGGCTGCTGATTTTTGGTATCAGc < 1:402326/69‑1 (MQ=255) ggTGCGCGCCTGCTGGAAACCGCGGCGGGTATTCCTTATGAAACCGGGCTGCTGATTTTTGGTATCAGc < 1:368979/69‑1 (MQ=255) ggTGCGCGCCTGCTGGAAACCGCGGCGGGTATTCCTTATGAAACCGGGCTGCTGATTTTTGGTATCAGc < 1:1909241/69‑1 (MQ=255) ggTGCGCGCCTGCTGGAAACCGCGGCGGGTATTCCTTATGAAACCGGGCTGCTGATTTTTGGTATCAGc < 1:1824287/69‑1 (MQ=255) ggTGCGCGCCTGCTGGAAACCGCGGCGGGTATTCCTTATGAAACCGGGCTGCTGATTTTTGGTATCAGc < 1:3125819/69‑1 (MQ=255) ggTGCGCGCCTGCTGGAAACCGCGGCGGGTATTCCTTATGAAACCGGGCTGCTGATTTTTGGTATCAGc < 1:1636142/69‑1 (MQ=255) tGCGCGCCTGCTGGAAACCGCGGCGGGTATTCCTTATGAAACCGGGCTGCTGATTTTTGGTATCAGCAt < 1:356599/69‑1 (MQ=255) tGCGCGCCTGCTGGAAACCGCGGCGGGTATTCCTTATGAAACCGGGCTGCTGATTTTTGGTATCAGCAt < 1:2623227/69‑1 (MQ=255) gcgcgcCTGCTGGAAACCGCGGCGGGTATTCCTTATGAAACCGGGCTGCTGATTTTTGGTATCAGCAtt > 1:2154299/1‑69 (MQ=255) gcCTGCTGGAAACCGCGGCGGGTATTCCTTATGAAACCGGGCTGCTGATTTTTGGTATCAGCATTGCGt < 1:1812441/69‑1 (MQ=255) gctgGAAACCGCGGCGGGTATTCCTTATGAAACCGGGCTGCTGATTTTTGGTATCAGCAt < 1:1112710/60‑1 (MQ=255) gAAACCGCGGCGGGTATTCCTTATGAAACCGGGCTGCTGATTTTTGGTATCAGc < 1:1005968/54‑1 (MQ=255) gAAACCGCGGCGGGTATTCCTTATGAAACCGGGCTGCTGATTTTTGGTATCAGCATTGCGTTATATAcc > 1:3342216/1‑69 (MQ=255) cggcggGTATTCCTTATGAAACCGGGCTGCTGATTTTCGGTATCAGCATTGCGTTGTATACCGCCTTtg < 1:3416784/69‑1 (MQ=255) cggcggGTATTCCTTATGAAACCGGGCTGCTGATTTTCGGTATCAGCATTGCGTTGTATACCGCCTTtg < 1:540768/69‑1 (MQ=255) cggcggGTATTCCTTATGAAACCGGGCTGCTGATTTTCGGTATCAGCATTGCGTTGTATACCGCCTTtg < 1:376917/69‑1 (MQ=255) cggcggGTATTCCTTATGAAACCGGGCTGCTGATTTTCGGTATCAGCATTGCGTTGTATACCGCCTTtg < 1:2487215/69‑1 (MQ=255) cggcggGTATTCCTTATGAAACCGGGCTGCTGATTTTCGGTATCAGCATTGCGTTGTATACCGCCTTtg < 1:2554256/69‑1 (MQ=255) cggcggGTATTCCTTATGAAACCGGGCTGCTGATTTTCGGTATCAGCATTGCGTTGTATACCGCCTTtg < 1:1049931/69‑1 (MQ=255) cggcggGTATTCCTTATGAAACCGGGCTGCTGATTTTCGGTATCAGCATTGCGTTGTATACCGCCTTtg < 1:116908/69‑1 (MQ=255) cggcggGTATTCCTTATGAAACCGGGCTGCTGATTTTCGGTATCAGCATTGCGTTGTATACCGCCTTtg < 1:1744701/69‑1 (MQ=255) cggcggGTATTCCTTATGAAACCGGGCTGCTGATTTTCGGTATCAGCATTGCGTTGTATACCGCCTTtg < 1:1289121/69‑1 (MQ=255) cggcggGTATTCCTTATGAAACCGGGCTGCTGATTTTCGGTATCAGCATTGCGTTGTATACCGCCTTtg < 1:1356164/69‑1 (MQ=255) cggcggGTATTCCTTATGAAACCGGGCTGCTGATTTTCGGTATCAGCATTGCGTTGTATACCGCCTTtg < 1:1624875/69‑1 (MQ=255) gggTATTCCTTATGAAACCGGGCTGCTGATTTTTGGTATCAGCATTGCGTTATATACCGCCTTtggtgg > 1:1054765/1‑69 (MQ=255) ccTTATGAAACCGGGCTGCTGATTTTTGGTATCAGCATTGCGTTATATACCGCCTTTGGTGGCTTTcgc > 1:798873/1‑69 (MQ=255) ccTTATGAAACCGGGCTGCTGATTTTTGGTATCAGCATTGCGTTATATACCGCCTTTGGTGGCTTTcgc > 1:2000214/1‑69 (MQ=255) ccTTATGAAACCGGGCTGCTGATTTTTGGTATCAGCATTGCGTTATATACCGCCTTTGGTGGCTTTcgc > 1:1942267/1‑69 (MQ=255) ccTTATGAAACCGGGCTGCTGATTTTTGGTATCAGCATTGCGTTATATACCGCCTTTGGTGGCTTTcgc > 1:3249285/1‑69 (MQ=255) ccTTATGAAACCGGGCTGCTGATTTTTGGTATCAGCATTGCGTTATATACCGCCTTTGGTGGCTTTcgc > 1:150459/1‑69 (MQ=255) cTTATGAAACCGGGCTGCTGATTTTTGGTATCAGc < 1:2538542/35‑1 (MQ=255) tATGAAGCCGGGCTGCTGATTTTCGGTATCAGCATTGCGTTGTATACCGCCttt > 1:436529/1‑54 (MQ=255) tATGAAACCGGGCTGCTGATTTTCGGTATCAGCATTGCGTTGTATACCGCCttt > 1:169972/1‑54 (MQ=255) tATGAAACCGGGCTGCTGATTTTCGGTATCAGCATTGCGTTGTATACCGCCttt > 1:127454/1‑54 (MQ=255) tATGAAACCGGGCTGCTGATTTTCGGTATCAGCATTGCGTTGTATACCGCCttt > 1:2409259/1‑54 (MQ=255) tATGAAACCGGGCTGCTGATTTTCGGTATCAGCATTGCGTTGTATACCGCCttt > 1:1816637/1‑54 (MQ=255) tATGAAACCGGGCTGCTGATTTTCGGTATCAGCATTGCGTTGTATACCGCCttt > 1:2860052/1‑54 (MQ=255) tATGAAACCGGGCTGCTGATTTTCGGTATCAGCATTGCGTTGTATACCGCCttt > 1:2337765/1‑54 (MQ=255) tATGAAAACGGGCTGCTGATTTTCGGTATCAGCATTGCGTTGTATAc > 1:797007/1‑47 (MQ=38) gAAACCGGGCTGCTGATTTTTGGTATCAGCATTGCGTTATATACCGCCTTTGTTGGCTTTCGCGCCAg < 1:3178958/68‑1 (MQ=255) gAAACCGGGCTGCTGATTTTTGGTATCAGCATTGCGTTATATACCGCCTTTGGTGGCTTTCGCGCCAg < 1:673971/68‑1 (MQ=255) gggCTGCTGATTTTTGGTATCAGCATTGCGTTATATACCGCCTTTGGTGGCTTTCGCGCCAGCGTGCTg < 1:3241884/69‑1 (MQ=255) gggCTGCTGATTTTTGGTATCAGCATTGCGTTATATACCGCCTTTGGTGGCTTTCGCGCCAGCGTGCTg < 1:553877/69‑1 (MQ=255) ggCTGCTGATTTTTGGTATCAGCATTGCGTTATATACCGCCTTTGGTGGCTTTCGCGCCAGCGTGCTg < 1:563470/68‑1 (MQ=255) ggCTGCTGATTTTTGGTATCAGCATTGCGTTATATACCGCCTTTGGTGGCTTTCGCGCCAGCGTGCTg < 1:2266077/68‑1 (MQ=255) ctgctgATTTTTGGTATCAGCATTGCGTTATATACCGCCTTTGGTGGCTTTCGCGCCAGCGTGCTGAAc < 1:3037812/69‑1 (MQ=255) ctgctgATTTTTGGTATCAGCATTGCGTTATATACCGCCTTTGGTGGCTTTCGCGCCAGCGTGCTGAAc < 1:2165985/69‑1 (MQ=255) | TTTATCGGCGGTGCGCGCCTGCTGGAAACCGCGGCGGGTATTCCTTATGAAACCGGGCTGCTGATTTTTGGTATCAGCATTGCGTTATATACCGCCTTTGGTGGCTTTCGCGCCAGCGTGCTGAAC > minE/2223315‑2223440 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |