| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,223,401 | A→G | 21.5% | L171L (TTA→TTG) | panF → | pantothenate:sodium symporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,223,401 | 0 | A | G | 21.5% | 119.4 / 24.6 | 88 | L171L (TTA→TTG) | panF | pantothenate:sodium symporter |
| Reads supporting (aligned to +/- strand): ref base A (44/25); new base G (8/11); total (52/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.16e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GAAACCGCGGCGGGTATTCCTTATGAAACCGGGCTGCTGATTTTTGGTATCAGCATTGCGTTATATACCGCCTTTGGTGGCTTTCGCGCCAGCGTGCTGAACGACACCATGCAAGGGCTTGTGATGCTGAT > minE/2223339‑2223469 | gAAACCGCGGCGGGTATTCCTTATGAAACCGGGCTGCTGATTTTTGGTATCAGCATTGCGTTATATAcc > 1:3342216/1‑69 (MQ=255) cggcggGTATTCCTTATGAAACCGGGCTGCTGATTTTCGGTATCAGCATTGCGTTGTATACCGCCTTtg < 1:540768/69‑1 (MQ=255) cggcggGTATTCCTTATGAAACCGGGCTGCTGATTTTCGGTATCAGCATTGCGTTGTATACCGCCTTtg < 1:376917/69‑1 (MQ=255) cggcggGTATTCCTTATGAAACCGGGCTGCTGATTTTCGGTATCAGCATTGCGTTGTATACCGCCTTtg < 1:1049931/69‑1 (MQ=255) cggcggGTATTCCTTATGAAACCGGGCTGCTGATTTTCGGTATCAGCATTGCGTTGTATACCGCCTTtg < 1:3416784/69‑1 (MQ=255) cggcggGTATTCCTTATGAAACCGGGCTGCTGATTTTCGGTATCAGCATTGCGTTGTATACCGCCTTtg < 1:1744701/69‑1 (MQ=255) cggcggGTATTCCTTATGAAACCGGGCTGCTGATTTTCGGTATCAGCATTGCGTTGTATACCGCCTTtg < 1:116908/69‑1 (MQ=255) cggcggGTATTCCTTATGAAACCGGGCTGCTGATTTTCGGTATCAGCATTGCGTTGTATACCGCCTTtg < 1:1624875/69‑1 (MQ=255) cggcggGTATTCCTTATGAAACCGGGCTGCTGATTTTCGGTATCAGCATTGCGTTGTATACCGCCTTtg < 1:1289121/69‑1 (MQ=255) cggcggGTATTCCTTATGAAACCGGGCTGCTGATTTTCGGTATCAGCATTGCGTTGTATACCGCCTTtg < 1:2487215/69‑1 (MQ=255) cggcggGTATTCCTTATGAAACCGGGCTGCTGATTTTCGGTATCAGCATTGCGTTGTATACCGCCTTtg < 1:1356164/69‑1 (MQ=255) cggcggGTATTCCTTATGAAACCGGGCTGCTGATTTTCGGTATCAGCATTGCGTTGTATACCGCCTTtg < 1:2554256/69‑1 (MQ=255) gggTATTCCTTATGAAACCGGGCTGCTGATTTTTGGTATCAGCATTGCGTTATATACCGCCTTtggtgg > 1:1054765/1‑69 (MQ=255) ccTTATGAAACCGGGCTGCTGATTTTTGGTATCAGCATTGCGTTATATACCGCCTTTGGTGGCTTTcgc > 1:1942267/1‑69 (MQ=255) ccTTATGAAACCGGGCTGCTGATTTTTGGTATCAGCATTGCGTTATATACCGCCTTTGGTGGCTTTcgc > 1:2000214/1‑69 (MQ=255) ccTTATGAAACCGGGCTGCTGATTTTTGGTATCAGCATTGCGTTATATACCGCCTTTGGTGGCTTTcgc > 1:150459/1‑69 (MQ=255) ccTTATGAAACCGGGCTGCTGATTTTTGGTATCAGCATTGCGTTATATACCGCCTTTGGTGGCTTTcgc > 1:3249285/1‑69 (MQ=255) ccTTATGAAACCGGGCTGCTGATTTTTGGTATCAGCATTGCGTTATATACCGCCTTTGGTGGCTTTcgc > 1:798873/1‑69 (MQ=255) tATGAAGCCGGGCTGCTGATTTTCGGTATCAGCATTGCGTTGTATACCGCCttt > 1:436529/1‑54 (MQ=255) tATGAAACCGGGCTGCTGATTTTCGGTATCAGCATTGCGTTGTATACCGCCttt > 1:2860052/1‑54 (MQ=255) tATGAAACCGGGCTGCTGATTTTCGGTATCAGCATTGCGTTGTATACCGCCttt > 1:127454/1‑54 (MQ=255) tATGAAACCGGGCTGCTGATTTTCGGTATCAGCATTGCGTTGTATACCGCCttt > 1:169972/1‑54 (MQ=255) tATGAAACCGGGCTGCTGATTTTCGGTATCAGCATTGCGTTGTATACCGCCttt > 1:2409259/1‑54 (MQ=255) tATGAAACCGGGCTGCTGATTTTCGGTATCAGCATTGCGTTGTATACCGCCttt > 1:2337765/1‑54 (MQ=255) tATGAAACCGGGCTGCTGATTTTCGGTATCAGCATTGCGTTGTATACCGCCttt > 1:1816637/1‑54 (MQ=255) tATGAAAACGGGCTGCTGATTTTCGGTATCAGCATTGCGTTGTATAc > 1:797007/1‑47 (MQ=38) gAAACCGGGCTGCTGATTTTTGGTATCAGCATTGCGTTATATACCGCCTTTGTTGGCTTTCGCGCCAg < 1:3178958/68‑1 (MQ=255) gAAACCGGGCTGCTGATTTTTGGTATCAGCATTGCGTTATATACCGCCTTTGGTGGCTTTCGCGCCAg < 1:673971/68‑1 (MQ=255) gggCTGCTGATTTTTGGTATCAGCATTGCGTTATATACCGCCTTTGGTGGCTTTCGCGCCAGCGTGCTg < 1:3241884/69‑1 (MQ=255) gggCTGCTGATTTTTGGTATCAGCATTGCGTTATATACCGCCTTTGGTGGCTTTCGCGCCAGCGTGCTg < 1:553877/69‑1 (MQ=255) ggCTGCTGATTTTTGGTATCAGCATTGCGTTATATACCGCCTTTGGTGGCTTTCGCGCCAGCGTGCTg < 1:2266077/68‑1 (MQ=255) ggCTGCTGATTTTTGGTATCAGCATTGCGTTATATACCGCCTTTGGTGGCTTTCGCGCCAGCGTGCTg < 1:563470/68‑1 (MQ=255) ctgctgATTTTTGGTATCAGCATTGCGTTATATACCGCCTTTGGTGGCTTTCGCGCCAGCGTGCTGAAc < 1:3037812/69‑1 (MQ=255) ctgctgATTTTTGGTATCAGCATTGCGTTATATACCGCCTTTGGTGGCTTTCGCGCCAGCGTGCTGAAc < 1:2165985/69‑1 (MQ=255) tCAGCATTGCGTTATATACCGCCTTTGGTGGCTTTCGCGCCAGCGTGCTGAACGACACCATGCa < 1:1736971/64‑1 (MQ=255) tCAGCATTGCGTTATATACCGCCTTTGGTGGCTTTCGCGCCAGCGTGCTGAACGACACCATGCa < 1:398629/64‑1 (MQ=255) tCAGCATTGCGTTATATACCGCCTTTGGTGGCTTTCGCGCCAGCGTGCTGAACGACACCATGCa < 1:2502854/64‑1 (MQ=255) tCAGCATTGCGTTATATACCGCCTTTGGTGGCTTTCGCGCCAGCGTGCTGAACGACACCATGCa < 1:3127122/64‑1 (MQ=255) tCAGCATTGCGTTATATACCGCCTTTGGTGGCTTTCGCGCCAGCGTGCTGAACGACACCATGCa < 1:2220343/64‑1 (MQ=255) aGCATTGCGTTATATACCGCCTTTGGTGGCTTTcgc > 1:2149325/1‑36 (MQ=255) aGCATTGCGTTATATACCGCCTTTGGTGGCTTTCGCGCCAGCGTGCTGAACGACACCATGCAAgg > 1:782156/1‑65 (MQ=255) aGCATTGCGTTATATACCGCCTTTGGTGGCTTTCGCGCCAGCGTGCTGAACGACACCATGCAAgg > 1:747471/1‑65 (MQ=255) aGCATTGCGTTATATACCGCCTTTGGTGGCTTTCGCGCCAGCGTGCTGAACGACACCATGCAAgg > 1:3347533/1‑65 (MQ=255) aGCATTGCGTTATATACCGCCTTTGGTGGCTTTCGCGCCAGCGTGCTGAACGACACCATGCAAgg > 1:510593/1‑65 (MQ=255) aGCATTGCGTTATATACCGCCTTTGGTGGCTTTCGCGCCAGCGTGCTGAACGACACCATGCAAgg > 1:508709/1‑65 (MQ=255) aGCATTGCGTTATATACCGCCTTTGGTGGCTTTCGCGCCAGCGTGCTGAACGACACCATGCAAgg > 1:1014031/1‑65 (MQ=255) aGCATTGCGTTATATACCGCCTTTGGTGGCTTTCGCGCCAGCGTGCTGAACGACACCATGCAAgg > 1:3065611/1‑65 (MQ=255) aGCATTGCGTTATATACCGCCTTTGGTGGCTTTCGCGCCAGCGTGCTGAACGACACCATGCAAgg > 1:1218705/1‑65 (MQ=255) aGCATTGCGTTATATACCGCCTTTGGTGGCTTTCGCGCCAGCGTGCTGAACGACACCATGCAAGGGCtt > 1:1001755/1‑69 (MQ=255) aGCATTGCGTTATATACCGCCTTTGGTGGCTTTCGCGCCAGCGTGCTGAACGACACCATGCAAGGGCtt > 1:1065710/1‑69 (MQ=255) aGCATTGCGTTATATACCGCCTTTGGTGGCTTTCGCGCCAGCGTGCTGAACGACACCATGCAAGGGCtt > 1:922326/1‑69 (MQ=255) aGCATTGCGTTATATACCGCCTTTGGTGGCTTTCGCGCCAGCGTGCTGAACGACACCATGCAAGGGCtt > 1:1828936/1‑69 (MQ=255) aGCATTGCGTTATATACCGCCTTTGGTGGCTTTCGCGCCAGCGTGCTGAACGACACCATGCAAGGGCtt > 1:630681/1‑69 (MQ=255) aGCATTGCGTTATATACCGCCTTTGGTGGCTTTCGCGCCAGCGTGCTGAACGACACCATGCAAGGGCtt > 1:658662/1‑69 (MQ=255) aGCATTGCGTTATATACCGCCTTTGGTGGCTTTCGCGCCAGCGTGCTGAACGACACCATGCAAGGGCtt > 1:663649/1‑69 (MQ=255) aGCATTGCGTTATATACCGCCTTTGGTGGCTTTCGCGCCAGCGTGCTGAACGACACCATGCAAGGGCtt > 1:2230088/1‑69 (MQ=255) aGCATTGCGTTATATACCGCCTTTGGTGGCTTTCGCGCCAGCGTGCTGAACGACACCATGCAAGGGCtt > 1:227861/1‑69 (MQ=255) aGCATTGCGTTATATACCGCCTTTGGTGGCTTTCGCGCCAGCGTGCTGAACGACACCATGCAAGGGCtt > 1:842489/1‑69 (MQ=255) aGCATTGCGTTATATACCGCCTTTGGTGGCTTTCGCGCCAGCGTGCTGAACGACACCATGCAAGGGCtt > 1:2463455/1‑69 (MQ=255) aGCATTGCGTTATATACCGCCTTTGGTGGCTTTCGCGCCAGCGTGCTGAACGACACCATGCAAGGGCtt > 1:1545942/1‑69 (MQ=255) aGCATTGCGTTATATACCGCCTTTGGTGGCTTTCGCGCCAGCGTGCTGAACGACACCATGCAAGGGCtt > 1:1522800/1‑69 (MQ=255) aGCATTGCGTTATATACCGCCTTTGGTGGCTTTCGCGCCAGCGTGCTGAACGACACCATGCAAGGGCtt > 1:2632497/1‑69 (MQ=255) aGCATTGCGTTATATACCGCCTTTGGTGGCTTTCGCGCCAGCGTGCTGAACGACACCATGCAAGGGCtt > 1:2748997/1‑69 (MQ=255) aGCATTGCGTTATATACCGCCTTTGGTGGCTTTCGCGCCAGCGTGCTGAACGACACCATGCAAGGGCtt > 1:2883003/1‑69 (MQ=255) aGCATTGCGTTATATACCGCCTTTGGTGGCTTTCGCGCCAGCGTGCTGAACGACACCATGCAAGGGCtt > 1:2157771/1‑69 (MQ=255) aGCATTGCGTTATATACCGCCTTTGGTGGCTTTCGCGCCAGCGTGCTGAACGACACCATGCAAGGGCtt > 1:1420406/1‑69 (MQ=255) aGCATTGCGTTATATACCGCCTTTGGTGGCTTTCGCGCCAGCGTGCTGAACGACACCATGCAAGGGCtt > 1:833229/1‑69 (MQ=255) aGCATTGCGTTATATACCGCCTTTGGTGGCTTTCGCGCCAGCGTGCTGAACGACACCATGCAAGGGCtt > 1:1854251/1‑69 (MQ=255) aGCATTGCGTTATATACCGCCTTTGGTGGCTTTCGCGCCAGCGTGCTGAACGACACCATGCAAGGGCtt > 1:858364/1‑69 (MQ=255) aGCATTGCGTTATATACCGCCTTTGGTGGCTTTCGCGCCAGCGTGCTGAACGACACCATGCAAGGGCt > 1:3290212/1‑68 (MQ=255) aGCATTGCGTTATATACCGCCTTTGGTGGCTTTCGCGCCAGCGTGCTGAACGACACCATGCAAGGGCt < 1:2234949/68‑1 (MQ=255) aGCATTGCGTTATATACCGCCTTTGGTGGCTTTCGCGCCAGCGTGCTGAACGACACCATGCAAGGGCt < 1:651002/68‑1 (MQ=255) aGCATTGCGTTATATACCGCCTTTGGTGGCTTTCGCGCCAGCGTGCTGAACGACACCATGCAAGGGCt < 1:2661874/68‑1 (MQ=255) aGCATTGCGTTATATACCGCCTTTGGTGGCTTTCGCGCCAGCGTGCTGAACGACACCATGCAAGGGCt < 1:1458451/68‑1 (MQ=255) aGCATTGCGTTATATACCGCCTTTGGTGGCTTTCGCGCCAGCGTGCTGAACGACACCATGCAAGGGCt < 1:1104451/68‑1 (MQ=255) aGCATTGCGTTATATACCGCCTTTGGTGGCTTTCGCGCCAGCGTGCTGAACGACACCATGCAAGGGCt < 1:3355305/68‑1 (MQ=255) aGCATTGCGTTATATACCGCCTTTGGTGGCTTTCGCGCCAGCGTGCTGAACGACACCATGCAAGGGCt < 1:1351621/68‑1 (MQ=255) aGCATTGCGTTATATACCGCCTTTGGTGGCTTTCGCGCCAGCGTGATGAACGACACCATGCAAgg > 1:958321/1‑65 (MQ=255) aGCATTGCGTTATATACCGCCTTTGGTGGATTTCGCGCCAGCGTGc > 1:3365239/1‑46 (MQ=255) cATTGCGTTATATACCGCCTTTGGTGGCTTTCGCGCCAGCGTGCTGAACGACACCATGCAAGGGCTtgt < 1:607014/69‑1 (MQ=255) cATTGCGTTATATACCGCCTTTGGTGGCTTTCGCGCCAGCGTGCTGAACGACACCATGCAAGGGCTtgt < 1:3319321/69‑1 (MQ=255) cATTGCGTTATATACCGCCTTTGGTGGCTTTCGCGCCAGCGTGCTGAACGACACCATGCAAGGGCTtgt < 1:1782758/69‑1 (MQ=255) cATTGCGTTATATACCGCCTTTGGTGGCTTTCGCGCCAGCGTGCTGAACGACACCATGCAAGGGCTtgt < 1:1410592/69‑1 (MQ=255) cATTGCGTTATATACCGCCTTTGGTGGCTTTCGCGCCAGCGTGCTGAACGACACCATGCAAGGGCTtgt < 1:898619/69‑1 (MQ=255) gCGTTATATACCGCCTTTGGTGGCTTTCGCGCCAGCGTGCTGAACGACACCATGCAAGGGCTTGTGATg > 1:547159/1‑69 (MQ=255) gCGTTATATACCGCCTTTGGTGGCTTTCGCGCCAGCGTGCTGAACGACACCATGCAAGGGCTTGTGATg > 1:1022476/1‑69 (MQ=255) gCGTTATATACCGCCTTTGGTGGCTTTCGCGCCAGCGTGCTGAACGACACCATGCAAGGGCTTGTGATg > 1:949523/1‑69 (MQ=255) gCGTTATATACCGCCTTTGGTGGCTTTCGCGCCAGCGTGCTGAACGACACCATGCAAGGGCTTGTGATg > 1:1013294/1‑69 (MQ=255) atataCCGCCTTTGGTGGCTTTCGCGCCAGCGTGCTGAACGACACCATGCAAGGGCTTGTGATGCTGAt > 1:3203041/1‑69 (MQ=255) | GAAACCGCGGCGGGTATTCCTTATGAAACCGGGCTGCTGATTTTTGGTATCAGCATTGCGTTATATACCGCCTTTGGTGGCTTTCGCGCCAGCGTGCTGAACGACACCATGCAAGGGCTTGTGATGCTGAT > minE/2223339‑2223469 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |