| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,224,918 | C→T | 42.7% | I189I (ATC→ATT) | prmA → | methylase for 50S ribosomal subunit protein L11 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,224,918 | 0 | C | T | 42.7% | 35.0 / 77.7 | 103 | I189I (ATC→ATT) | prmA | methylase for 50S ribosomal subunit protein L11 |
| Reads supporting (aligned to +/- strand): ref base C (35/24); new base T (18/26); total (53/50) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.53e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01 | |||||||||||
Only 100 of 103 total aligned reads displayed.
GTTCCGGCATTCTGGCGATCGCGGCGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGTAATGGCGTTTCTGACCGTCTGGAA > minE/2224854‑2224984 | gTTCCGGCATTCTGGCGATCGCGGCGCTGAAACTTGGTGCAGCAAAAGCCATTGGTATTGATATCGATc < 1:1053640/69‑1 (MQ=255)gTTCCGGCATTCTGGCGATCGCGGCGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGa < 1:2093219/67‑1 (MQ=255)gTTCCGGCATTCTGGCGATCGCGGCGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATc < 1:54801/69‑1 (MQ=255)gTTCCGGCATTCTGGCGATCGCGGCGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATc < 1:1590323/69‑1 (MQ=255)gTTCCGGCATTCTGGCGATCGCGGCGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATc < 1:124739/69‑1 (MQ=255) cTGGCGATCGCGGCGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGa > 1:1234776/1‑67 (MQ=255) cTGGCGATCGCGGCGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGa > 1:7126/1‑67 (MQ=255) cTGGCGATCGCGGCGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGa > 1:3208424/1‑67 (MQ=255) cTGGCGATCGCGGCGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGa > 1:2525692/1‑67 (MQ=255) cTGGCGATCGCGGCGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGa > 1:2537379/1‑67 (MQ=255) cTGGCGATCGCGGCGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGa > 1:2120610/1‑67 (MQ=255) cTGGCGATCGCGGCGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGa > 1:1148987/1‑67 (MQ=255) cTGGCGATCGCGGCGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGa > 1:1033082/1‑67 (MQ=255) aTCGCGGCGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATTGATCCGCAGGCg < 1:2282295/60‑1 (MQ=255) aTCGCGGCGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATTGATCCGCAGGCg < 1:2366572/60‑1 (MQ=255) aTCGCGGCGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATTGATCCGCAGGCg < 1:1874773/60‑1 (MQ=255) aTCGCGGCGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATTGATCCGCAGGCg < 1:408008/60‑1 (MQ=255) aTCGCGGCGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATTGATCCGCAGGCg < 1:2351692/60‑1 (MQ=255) aTCGCGGCGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATTGATCCGCAGGCg < 1:1440407/60‑1 (MQ=255) aTCGCGGCGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATTGATCCGCAGGCg < 1:205372/60‑1 (MQ=255) aTCGCGGCGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATTGATCCGCAGGCg < 1:2179010/60‑1 (MQ=255) aTCGCGGCGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATTGATCCGCAGGCg < 1:3231976/60‑1 (MQ=255) aTCGCGGCGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATTGATCCGCAGGCg < 1:2416511/60‑1 (MQ=255) aTCGCGGCGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATTGATCCGCAGGCg < 1:567257/60‑1 (MQ=255) aTCGCGGCGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATTGATCCGCAGGCg < 1:2439/60‑1 (MQ=255) aTCGCGGCGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATTGATCCGCAGGCg < 1:2447789/60‑1 (MQ=255) aTCGCGGCGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATTGATCCGCAGGCg < 1:3052580/60‑1 (MQ=255) aTCGCGGCGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATTGATCCGCAGGCg < 1:2714528/60‑1 (MQ=255) aTCGCGGCGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATTGATCCGCAGGCg < 1:825480/60‑1 (MQ=255) aTCGCGGCGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATTGATCCGCAGGCg < 1:2817314/60‑1 (MQ=255) aTCGCGGCGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATTGATCCGCAGGCg < 1:2912201/60‑1 (MQ=255) aTCGCGGCGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATTGATCCGCAGGCg < 1:925291/60‑1 (MQ=255) tGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGCCCAGCCGCGAt < 1:2940307/68‑1 (MQ=255) gAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGAt < 1:807084/67‑1 (MQ=255) gAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGAt < 1:3215457/67‑1 (MQ=255) gAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGAt < 1:3059381/67‑1 (MQ=255) gAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGAt < 1:1369559/67‑1 (MQ=255) gAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGAt < 1:1404458/67‑1 (MQ=255) gAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATaa < 1:1284749/69‑1 (MQ=255) gAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATaa < 1:1962306/69‑1 (MQ=255) gAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATaa < 1:791071/69‑1 (MQ=255) aaaCTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATaa < 1:2741754/68‑1 (MQ=255) tGGGTGCAGCAAAAGCCATTGGTATTGATATCGATcc > 1:2950565/1‑37 (MQ=255) tGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGAt > 1:3023270/1‑46 (MQ=255) tGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCg > 1:705883/1‑69 (MQ=255) tGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCg > 1:2772431/1‑69 (MQ=255) tGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCg > 1:3157534/1‑69 (MQ=255) tGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCg > 1:2322007/1‑69 (MQ=255) tGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCg > 1:1203342/1‑69 (MQ=255) tGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGaa < 1:592433/67‑1 (MQ=255) tGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGaa < 1:2572042/67‑1 (MQ=255) gcagcaAAAGCCATTGTTATTGATATTGATCCGCAGGCGATTCAGGCCAGCCGCGACAACGCTGAACGt < 1:3259328/69‑1 (MQ=255) gcagcaAAAGCCATTGGTATTGATATTGATCCGCAGGCGATTCAGGCCAGCCGCGACAACGCTGAACGt < 1:1006006/69‑1 (MQ=255) gcagcaAAAGCCATTGGTATTGATATTGATCCGCAGGCGATTCAGGCCAGCCGCGACAACGCTGAACGt < 1:2718619/69‑1 (MQ=255) gcagcaAAAGCCATTGGTATTGATATTGATCCGCAGGCGATTCAGGCCAGCCGCGACAACGCTGAACGt < 1:3096298/69‑1 (MQ=255) gcagcaAAAGCCATTGGTATTGATATTGATCCGCAGGCGATTCAGGCCAGCCGCGACAACGCTGAACGt < 1:3404743/69‑1 (MQ=255) gcagcaAAAGCCATTGGTATTGATATTGATCCGCAGGCGATTCAGGCCAGCCGCGACAACGCTGAACGt < 1:2139701/69‑1 (MQ=255) gcagcaAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGt < 1:564507/69‑1 (MQ=255) gcagcaAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGt < 1:3312036/69‑1 (MQ=255) gcagcaAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGt < 1:887969/69‑1 (MQ=255) gcagcaAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGt < 1:2654362/69‑1 (MQ=255) gcagcaAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGt < 1:1217643/69‑1 (MQ=255) gcagcaAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGt < 1:2515695/69‑1 (MQ=255) agcaAAAGCCATTGGTATTGATATTGATCCGCAGGCGATTCa > 1:1742983/1‑42 (MQ=255) agcaAAAGCCATTGGTATTGATATTGATCCGCAGGCGATTCAGGCCAGCCGCGACAAc > 1:360222/1‑58 (MQ=255) agcaAAAGCCATTGGTATTGATATTGATCCGCAGGCGATTCAGGCCAGCCGCGACAACGCTGAACGTaa > 1:2986724/1‑69 (MQ=255) agcaAAAGCCATTGGTATTGATATTGATCCGCAGGCGATTCAGGCCAGCCGCGACAACGCTGAACGTaa > 1:1029492/1‑69 (MQ=255) agcaAAAGCCATTGGTATTGATATTGATCCGCAGGCGATTCAGGCCAGCCGCGACAACGCTGAACGTaa > 1:1183518/1‑69 (MQ=255) agcaAAAGCCATTGGTATTGATATTGATCCGCAGGCGATTCAGGCCAGCCGCGACAACGCTGAACGTaa > 1:1360518/1‑69 (MQ=255) agcaAAAGCCATTGGTATTGATATTGATCCGCAGGCGATTCAGGCCAGCCGCGACAACGCTGAACGTaa > 1:737843/1‑69 (MQ=255) agcaAAAGCCATTGGTATTGATATTGATCCGCAGGCGATTCAGGCCAGCCGCGACAACGCTGAACGTaa > 1:1452275/1‑69 (MQ=255) agcaAAAGCCATTGGTATTGATATTGATCCGCAGGCGATTCAGGCCAGCCGCGACAACGCTGAACGTaa > 1:1611452/1‑69 (MQ=255) agcaAAAGCCATTGGTATTGATATTGATCCGCAGGCGATTCAGGCCAGCCGCGACAACGCTGAACGTaa > 1:565986/1‑69 (MQ=255) agcaAAAGCCATTGGTATTGATATTGATCCGCAGGCGATTCAGGCCAGCCGCGACAACGCTGAACGTaa > 1:419927/1‑69 (MQ=255) agcaAAAGCCATTGGTATTGATATTGATCCGCAGGCGATTCAGGCCAGCCGCGACAACGCTGAACGTaa > 1:2628509/1‑69 (MQ=255) agcaAAAGCCATTGGTATTGATATTGATCCGCAGGCGATTCAGGCCAGCCGCGACAACGCTGAACGTaa > 1:2117780/1‑69 (MQ=255) agcaAAAGCCATTGGTATTGATATTGATCCGCAGGCGATTCAGGCCAGCCGCGACAACGCTGAACGTaa > 1:2812545/1‑69 (MQ=255) agcaAAAGCCATTGGTATTGATATTGATCCGCAGGCGATTCAGGCCAGCCGCGACAACGCTGAACGTaa > 1:2141375/1‑69 (MQ=255) agcaAAAGCCATTGGTATTGATATTGATCCGCAGGCGATTCAGGCCAGCCGCGACAACGCTGAACGTaa > 1:2594231/1‑69 (MQ=255) agcaAAAGCCATTGGTATTGATATTGATCCGCAGGCGATTCAGGCCAGCCGCGACAACGCTGAACGTaa > 1:2668988/1‑69 (MQ=255) agcaAAAGCCATTGGTATTGATATTGATCCGCAGGCGATTAAGGCCAGCCGCGACAACGCTGAACGTaa > 1:1428918/1‑69 (MQ=255) aaaGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGAt > 1:785658/1‑51 (MQ=255) aaaGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGAt > 1:1389754/1‑51 (MQ=255) aaaGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGAt > 1:1443255/1‑51 (MQ=255) aaaGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGAt > 1:3123034/1‑51 (MQ=255) aaaGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGAt > 1:1643738/1‑51 (MQ=255) aaaGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGAt > 1:3234379/1‑51 (MQ=255) aaaGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGAt > 1:3246242/1‑51 (MQ=255) aaaGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGTAATGGc > 1:1981995/1‑69 (MQ=255) aaaGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGTAATGGc > 1:3360304/1‑69 (MQ=255) aaaGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGTAATGGc > 1:1982804/1‑69 (MQ=255) ggTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGTAATGGCGTTTCTGAc > 1:760232/1‑69 (MQ=255) atCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGTAATgcg > 1:2865622/1‑49 (MQ=255) atCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGTAATGGCGTTTCTGACCGTCTGGaa > 1:657575/1‑69 (MQ=255) atCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGTAATGGCGTTTCTGACCGTCTGGaa > 1:787211/1‑69 (MQ=255) atCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGTAATGGCGTTTCTGACCGTCTGGaa > 1:740937/1‑69 (MQ=255) atCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGTAATGGCGTTTCTGACCGTCTGGaa > 1:2601749/1‑69 (MQ=255) atCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGTAATGGCGTTTCTGACCGTCTGGaa > 1:1978515/1‑69 (MQ=255) atCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGTAATGGCGTTTCTGACCGTCTGGaa > 1:3282971/1‑69 (MQ=255) atCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGTAATGGCGTTTCTGACCGTCTGGa > 1:2271410/1‑68 (MQ=255) | GTTCCGGCATTCTGGCGATCGCGGCGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGTAATGGCGTTTCTGACCGTCTGGAA > minE/2224854‑2224984 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |