| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,224,948 | T→C | 57.4% | D199D (GAT→GAC) | prmA → | methylase for 50S ribosomal subunit protein L11 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,224,948 | 0 | T | C | 57.4% | 25.7 / 62.0 | 82 | D199D (GAT→GAC) | prmA | methylase for 50S ribosomal subunit protein L11 |
| Reads supporting (aligned to +/- strand): ref base T (19/16); new base C (17/30); total (36/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.20e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGTAATGGCGTTTCTGACCGTCTGGAACTCTACTTACCGAAAGATCAGCCAGAAGA > minE/2224881‑2225013 | tGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGCCCAGCCGCGAt < 1:2940307/68‑1 (MQ=255) gAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGAt < 1:807084/67‑1 (MQ=255) gAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGAt < 1:3059381/67‑1 (MQ=255) gAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGAt < 1:1369559/67‑1 (MQ=255) gAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGAt < 1:3215457/67‑1 (MQ=255) gAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGAt < 1:1404458/67‑1 (MQ=255) gAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATaa < 1:791071/69‑1 (MQ=255) gAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATaa < 1:1284749/69‑1 (MQ=255) gAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATaa < 1:1962306/69‑1 (MQ=255) aaaCTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATaa < 1:2741754/68‑1 (MQ=255) tGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCg > 1:2322007/1‑69 (MQ=255) tGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCg > 1:2772431/1‑69 (MQ=255) tGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCg > 1:3157534/1‑69 (MQ=255) tGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCg > 1:705883/1‑69 (MQ=255) tGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCg > 1:1203342/1‑69 (MQ=255) tGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGaa < 1:2572042/67‑1 (MQ=255) tGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGaa < 1:592433/67‑1 (MQ=255) gcagcaAAAGCCATTGTTATTGATATTGATCCGCAGGCGATTCAGGCCAGCCGCGACAACGCTGAACGt < 1:3259328/69‑1 (MQ=255) gcagcaAAAGCCATTGGTATTGATATTGATCCGCAGGCGATTCAGGCCAGCCGCGACAACGCTGAACGt < 1:2718619/69‑1 (MQ=255) gcagcaAAAGCCATTGGTATTGATATTGATCCGCAGGCGATTCAGGCCAGCCGCGACAACGCTGAACGt < 1:2139701/69‑1 (MQ=255) gcagcaAAAGCCATTGGTATTGATATTGATCCGCAGGCGATTCAGGCCAGCCGCGACAACGCTGAACGt < 1:3096298/69‑1 (MQ=255) gcagcaAAAGCCATTGGTATTGATATTGATCCGCAGGCGATTCAGGCCAGCCGCGACAACGCTGAACGt < 1:3404743/69‑1 (MQ=255) gcagcaAAAGCCATTGGTATTGATATTGATCCGCAGGCGATTCAGGCCAGCCGCGACAACGCTGAACGt < 1:1006006/69‑1 (MQ=255) gcagcaAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGt < 1:3312036/69‑1 (MQ=255) gcagcaAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGt < 1:2654362/69‑1 (MQ=255) gcagcaAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGt < 1:564507/69‑1 (MQ=255) gcagcaAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGt < 1:2515695/69‑1 (MQ=255) gcagcaAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGt < 1:887969/69‑1 (MQ=255) gcagcaAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGt < 1:1217643/69‑1 (MQ=255) agcaAAAGCCATTGGTATTGATATTGATCCGCAGGCGATTCAGGCCAGCCGCGACAAc > 1:360222/1‑58 (MQ=255) agcaAAAGCCATTGGTATTGATATTGATCCGCAGGCGATTCAGGCCAGCCGCGACAACGCTGAACGTaa > 1:1452275/1‑69 (MQ=255) agcaAAAGCCATTGGTATTGATATTGATCCGCAGGCGATTCAGGCCAGCCGCGACAACGCTGAACGTaa > 1:737843/1‑69 (MQ=255) agcaAAAGCCATTGGTATTGATATTGATCCGCAGGCGATTCAGGCCAGCCGCGACAACGCTGAACGTaa > 1:2668988/1‑69 (MQ=255) agcaAAAGCCATTGGTATTGATATTGATCCGCAGGCGATTCAGGCCAGCCGCGACAACGCTGAACGTaa > 1:2117780/1‑69 (MQ=255) agcaAAAGCCATTGGTATTGATATTGATCCGCAGGCGATTCAGGCCAGCCGCGACAACGCTGAACGTaa > 1:1611452/1‑69 (MQ=255) agcaAAAGCCATTGGTATTGATATTGATCCGCAGGCGATTCAGGCCAGCCGCGACAACGCTGAACGTaa > 1:2628509/1‑69 (MQ=255) agcaAAAGCCATTGGTATTGATATTGATCCGCAGGCGATTCAGGCCAGCCGCGACAACGCTGAACGTaa > 1:419927/1‑69 (MQ=255) agcaAAAGCCATTGGTATTGATATTGATCCGCAGGCGATTCAGGCCAGCCGCGACAACGCTGAACGTaa > 1:2594231/1‑69 (MQ=255) agcaAAAGCCATTGGTATTGATATTGATCCGCAGGCGATTCAGGCCAGCCGCGACAACGCTGAACGTaa > 1:2986724/1‑69 (MQ=255) agcaAAAGCCATTGGTATTGATATTGATCCGCAGGCGATTCAGGCCAGCCGCGACAACGCTGAACGTaa > 1:2812545/1‑69 (MQ=255) agcaAAAGCCATTGGTATTGATATTGATCCGCAGGCGATTCAGGCCAGCCGCGACAACGCTGAACGTaa > 1:1183518/1‑69 (MQ=255) agcaAAAGCCATTGGTATTGATATTGATCCGCAGGCGATTCAGGCCAGCCGCGACAACGCTGAACGTaa > 1:1360518/1‑69 (MQ=255) agcaAAAGCCATTGGTATTGATATTGATCCGCAGGCGATTCAGGCCAGCCGCGACAACGCTGAACGTaa > 1:565986/1‑69 (MQ=255) agcaAAAGCCATTGGTATTGATATTGATCCGCAGGCGATTCAGGCCAGCCGCGACAACGCTGAACGTaa > 1:2141375/1‑69 (MQ=255) agcaAAAGCCATTGGTATTGATATTGATCCGCAGGCGATTCAGGCCAGCCGCGACAACGCTGAACGTaa > 1:1029492/1‑69 (MQ=255) agcaAAAGCCATTGGTATTGATATTGATCCGCAGGCGATTAAGGCCAGCCGCGACAACGCTGAACGTaa > 1:1428918/1‑69 (MQ=255) aaaGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGAt > 1:1643738/1‑51 (MQ=255) aaaGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGAt > 1:3123034/1‑51 (MQ=255) aaaGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGAt > 1:1389754/1‑51 (MQ=255) aaaGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGAt > 1:785658/1‑51 (MQ=255) aaaGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGAt > 1:1443255/1‑51 (MQ=255) aaaGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGAt > 1:3234379/1‑51 (MQ=255) aaaGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGAt > 1:3246242/1‑51 (MQ=255) aaaGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGTAATGGc > 1:3101952/1‑69 (MQ=255) aaaGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGTAATGGc > 1:3360304/1‑69 (MQ=255) aaaGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGTAATGGc > 1:1981995/1‑69 (MQ=255) aaaGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGTAATGGc > 1:1982804/1‑69 (MQ=255) ggTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGTAATGGCGTTTCTGAc > 1:760232/1‑69 (MQ=255) atCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGTAATgcg > 1:2865622/1‑49 (MQ=255) atCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGTAATGGCGTTTCTGACCGTCTGGaa > 1:657575/1‑69 (MQ=255) atCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGTAATGGCGTTTCTGACCGTCTGGaa > 1:1978515/1‑69 (MQ=255) atCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGTAATGGCGTTTCTGACCGTCTGGaa > 1:740937/1‑69 (MQ=255) atCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGTAATGGCGTTTCTGACCGTCTGGaa > 1:3282971/1‑69 (MQ=255) atCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGTAATGGCGTTTCTGACCGTCTGGaa > 1:2601749/1‑69 (MQ=255) atCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGTAATGGCGTTTCTGACCGTCTGGaa > 1:787211/1‑69 (MQ=255) atCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGTAATGGCGTTTCTGACCGTCTGGa > 1:2271410/1‑68 (MQ=255) gATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGTAATGGCGTTTCTGACCGTCTGGAActc < 1:3073289/69‑1 (MQ=255) aTCCGCAGGCGATTCAGGCCAGCCGCGACAACGCTGAACGTAATGGCGTTTCTg < 1:3084247/54‑1 (MQ=255) aTCCGCAGGCGATTCAGGCCAGCCGCGACAACGCTGAACGTAATGGCGTTTCTg < 1:573600/54‑1 (MQ=255) aTCCGCAGGCGATTCAGGCCAGCCGCGACAACGCTGAACGTAATGGCGTTTCTg < 1:826054/54‑1 (MQ=255) aTCCGCAGGCGATTCAGGCCAGCCGCGACAACGCTGAACGTAATGGCGTTTCTg < 1:1178271/54‑1 (MQ=255) aTCCGCAGGCGATTCAGGCCAGCCGCGACAACGCTGAACGTAATGGCGTTTCTg < 1:756469/54‑1 (MQ=255) aTCCGCAGGCGATTCAGGCCAGCCGCGACAACGCTGAACGTAATGGCGTTTCTg < 1:1335138/54‑1 (MQ=255) aTCCGCAGGCGATTCAGGCCAGCCGCGACAACGCTGAACGTAATGGCGTTTCTg < 1:2778577/54‑1 (MQ=255) aTCCGCAGGCGATTCAGGCCAGCCGCGACAACGCTGAACGTAATGGCGTTTCTg < 1:2094606/54‑1 (MQ=255) aTCCGCAGGCGATTCAGGCCAGCCGCGACAACGCTGAACGTAATGGCGTTTCTg < 1:2506879/54‑1 (MQ=255) aTCCGCAGGCGATTCAGGCCAGCCGCGACAACGCTGAACGTAATGGCGTTTCTg < 1:2050408/54‑1 (MQ=255) aTCCGCAGGCGATTCAGGCCAGCCGCGACAACGCTGAACGTAATGGCGTTTCTg < 1:1985899/54‑1 (MQ=255) aTCCGCAGGCGATTCAGGCCAGCCGCGACAACGCTGAACGTAATGGCGTTTCTg < 1:2641055/54‑1 (MQ=255) aTCCGCAGGCGATTCAGGCCAGCCGCGACAACGCTGAACGTAATGGCGTTTCTg < 1:2688792/54‑1 (MQ=255) aTCCGCAGGCGATTCAGGCCAGCCGCGACAACGCTGAACGTAATGGCGTTTCTg < 1:2711643/54‑1 (MQ=255) aTCCGCAGGCGATTCAGGCCAGCCGCGACAACGCTGAACGTAATGGCGTTTCTg < 1:1428525/54‑1 (MQ=255) aTCCGCAGGCGATTCAGGCCAGCCGCGACAACGCTGAACGTAATGGCGTTTCTg < 1:1787586/54‑1 (MQ=255) aTCCGCAGGCGATTCAGGCCAGCCGCGACAACGCTGAACGTAATGGCGTTTCTg < 1:2930166/54‑1 (MQ=255) aTCCGCAGGCGATTCAGGCCAGCCGCGACAACGCTGAACGTAATGGCGTTTCTg < 1:2946853/54‑1 (MQ=255) aTCCGCAGGCGATTCAGGCCAGCCGCGACAACGCTGAACGTAATGGCGTTTCTg < 1:2960758/54‑1 (MQ=255) aTCCGCAGGCGATTCAGGCCAGCCGCGACAACGCTGAACGTAATGGCGTTTCTg < 1:2307810/54‑1 (MQ=255) aTCCGCAGGCGATTCAGGCCAGCCGCGACAACGCTGAACGTAATGGCGTTTCTg < 1:222063/54‑1 (MQ=255) aTCCGCAGGCGATTCAGGCCAGCCGCGACAACGCTGAACGTAATGGCGTTTCTg < 1:1476745/54‑1 (MQ=255) aTCCGCAGGCGATTCAGGCCAGCCGCGACAACGCTGAACGTAATGGCGTTTCTg < 1:3150546/54‑1 (MQ=255) aTCCGCAGGCGATTCAGGCCAGCCGCGACAACGCTGAACGTAATGGCGTTTCTg < 1:3310504/54‑1 (MQ=255) cgcgATAACGCCGAACGTAATGGCGTTTCTGACCGTCTGGAACTCTACTTACCGAAAGATCAGCCaga > 1:2716268/1‑68 (MQ=255) cgATAACGCCGAACGTAATGGCGTTTCTGACCGTCTGGAACTCTACTTACCGAAAGATCAGCCagaaga < 1:450196/69‑1 (MQ=255) cgATAACGCCGAACGTAATGGCGTTTCTGACCGTCTGGAACTCTACTTACCGAAAGATCAGCCagaaga < 1:2998952/69‑1 (MQ=255) cgATAACGCCGAACGTAATGGCGTTTCTGACCGTCTGGAACTCTACTTACCGAAAGATCAGCCagaaga < 1:2108712/69‑1 (MQ=255) | TGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGTAATGGCGTTTCTGACCGTCTGGAACTCTACTTACCGAAAGATCAGCCAGAAGA > minE/2224881‑2225013 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |