| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,225,978 | G→A | 45.9% | K139K (AAG→AAA) | dusB → | tRNA‑dihydrouridine synthase B |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,225,978 | 0 | G | A | 45.9% | 8.4 / 48.1 | 61 | K139K (AAG→AAA) | dusB | tRNA‑dihydrouridine synthase B |
| Reads supporting (aligned to +/- strand): ref base G (14/19); new base A (22/6); total (36/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.36e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.01e-01 | |||||||||||
AAATCGATCCTTACCGAGGTCGTGAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAA > minE/2225925‑2226038 | aaaTCGATCCTTACCGAGGTCGTGAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTg > 1:2661704/1‑68 (MQ=255)aaaTCGATCCTTACCGAGGTCGTGAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTg > 1:1033450/1‑68 (MQ=255)aaaTCGATCCTTACCGAGGTCGTGAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTg > 1:2421161/1‑68 (MQ=255)aaaTCGATCCTTACCGAGGTCGTGAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTg > 1:790609/1‑68 (MQ=255)aaaTCGATCCTTACCGAGGTCGTGAATGCAGTGGACGTTCCTGTTACCCTGAAGATT‑‑CACCGGCTGGGc > 1:1626182/1‑69 (MQ=255) aTCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAAATTCGCACTGGCTGGGCAcc > 1:2609043/1‑68 (MQ=255) aTCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAAATTCGCACTGGCTGGGCAcc > 1:2143936/1‑68 (MQ=255) aTCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAAATTCGCACTGGCTGGGCAcc > 1:3004765/1‑68 (MQ=255) aTCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAAATTCGCACTGGCTGGGCAcc > 1:2329604/1‑68 (MQ=255) aTCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAAATTCGCACTGGCTGGGCAcc > 1:2525067/1‑68 (MQ=255) cTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAAATTCGCACTGGCTGGGCACCGGaa < 1:947190/69‑1 (MQ=255) cTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAAATTCGCACTGGCTGGGCACCGGaa < 1:2014047/69‑1 (MQ=255) cTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAAATTCGCACTGGCTGGGCACCGGaa < 1:2328945/69‑1 (MQ=255) cTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAAATTCGCACTGGCTGGGCACCGGaa < 1:2710257/69‑1 (MQ=255) cTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAAATTCGCACTGGCTGGGCACCGGaa < 1:2822652/69‑1 (MQ=255) cTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAAATTCGCACTGGCTGGGCACCGGaa < 1:3189003/69‑1 (MQ=255) gAGGTCGTGAATTCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGt < 1:1769532/69‑1 (MQ=255) gAGGTCGTGAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGt < 1:1263782/69‑1 (MQ=255) gAGGTCGTGAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGt < 1:2158738/69‑1 (MQ=255) aaTGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCg > 1:903014/1‑67 (MQ=255) gCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAagag < 1:2668861/69‑1 (MQ=255) gCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAagag < 1:2206666/69‑1 (MQ=255) gCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAagag < 1:3323388/69‑1 (MQ=255) gCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAagag < 1:1511223/69‑1 (MQ=255) gCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAagag < 1:1463323/69‑1 (MQ=255) gCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAagag < 1:984660/69‑1 (MQ=255) gACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTa > 1:2076187/1‑52 (MQ=255) gACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTa > 1:2054006/1‑52 (MQ=255) gACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTa > 1:353047/1‑52 (MQ=255) cGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCa < 1:198105/69‑1 (MQ=255) gTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCaa > 1:2015761/1‑69 (MQ=255) gTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCaa > 1:2545063/1‑69 (MQ=255) ccTGTTACCTTGAAAATTCGCACTGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTg > 1:2849254/1‑69 (MQ=255) ccTGTTACCCTGAAAATTCGCACTGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACt > 1:403106/1‑68 (MQ=255) ccTGTTACCCTGAAAATTCGCACTGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTg > 1:2369522/1‑69 (MQ=255) ccTGTTACCCTGAAAATTCGCACTGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTg > 1:649785/1‑69 (MQ=255) ccTGTTACCCTGAAAATTCGCACTGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTg > 1:2703435/1‑69 (MQ=255) ccTGTTACCCTGAAAATTCGCACTGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTg > 1:974761/1‑69 (MQ=255) ccTGTTACCCTGAAAATTCGCACTGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTg > 1:2625563/1‑69 (MQ=255) ccTGTTACCCTGAAAATTCGCACTGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTg > 1:2468346/1‑69 (MQ=255) ccTGTTACCCTGAAAATTCGCACTGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTg > 1:2422673/1‑69 (MQ=255) ccTGTTACCCTGAAAATTCGCACTGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTg > 1:1149743/1‑69 (MQ=255) ccTGTTACCCTGAAAATTCGCACTGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTg > 1:1491769/1‑69 (MQ=255) ccTGTTACCCTGAAAATTCGCACTGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTg > 1:2191611/1‑69 (MQ=255) ccTGTTACCCTGAAAATTCGCACTGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTg > 1:2179207/1‑69 (MQ=255) ccTGTTACCCTGAAAATTCGCACTGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTg > 1:2109032/1‑69 (MQ=255) ccTGTTACCCTGAAAATTCGCACTGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTg > 1:2581243/1‑69 (MQ=255) ccTGTTACCCTGAAAATTCGCACTGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTg > 1:2030592/1‑69 (MQ=255) ccTGTTACCCTGAAAATTCGCACTGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTg > 1:186263/1‑69 (MQ=255) aCCCTGAAGATTCGCACCGGCTGGGCACCGGACCACCGTAACTGCGAAGAGATTGCCCAACTGGCTGaa > 1:2854273/1‑69 (MQ=255) aCCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGaa < 1:2100083/69‑1 (MQ=255) aCCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGaa < 1:1121207/69‑1 (MQ=255) aCCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGaa > 1:1321696/1‑69 (MQ=255) aCCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGaa < 1:1751070/69‑1 (MQ=255) aCCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGaa < 1:437720/69‑1 (MQ=255) aCCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGaa < 1:415879/69‑1 (MQ=255) aCCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGaa > 1:1941036/1‑69 (MQ=255) aCCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGaa < 1:2198016/69‑1 (MQ=255) aCCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGaa < 1:2720583/69‑1 (MQ=255) aCCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGaa < 1:2262521/69‑1 (MQ=255) aCCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGaa < 1:2578043/69‑1 (MQ=255) | AAATCGATCCTTACCGAGGTCGTGAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAA > minE/2225925‑2226038 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |