| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,225,987 | C→T | 43.8% | T142T (ACC→ACT) | dusB → | tRNA‑dihydrouridine synthase B |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,225,987 | 0 | C | T | 43.8% | 22.9 / 62.3 | 82 | T142T (ACC→ACT) | dusB | tRNA‑dihydrouridine synthase B |
| Reads supporting (aligned to +/- strand): ref base C (27/19); new base T (30/6); total (57/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.85e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.48e-01 | |||||||||||
AAATCGATCCTTACCGAGGTCGTGAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGC > minE/2225925‑2226055 | aaaTCGATCCTTACCGAGGTCGTGAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTg > 1:2421161/1‑68 (MQ=255)aaaTCGATCCTTACCGAGGTCGTGAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTg > 1:1033450/1‑68 (MQ=255)aaaTCGATCCTTACCGAGGTCGTGAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTg > 1:790609/1‑68 (MQ=255)aaaTCGATCCTTACCGAGGTCGTGAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTg > 1:2661704/1‑68 (MQ=255)aaaTCGATCCTTACCGAGGTCGTGAATGCAGTGGACGTTCCTGTTACCCTGAAGATT‑‑CACCGGCTGGGc > 1:1626182/1‑69 (MQ=255) aTCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAAATTCGCACTGGCTGGGCAcc > 1:2329604/1‑68 (MQ=255) aTCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAAATTCGCACTGGCTGGGCAcc > 1:2525067/1‑68 (MQ=255) aTCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAAATTCGCACTGGCTGGGCAcc > 1:2609043/1‑68 (MQ=255) aTCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAAATTCGCACTGGCTGGGCAcc > 1:2143936/1‑68 (MQ=255) aTCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAAATTCGCACTGGCTGGGCAcc > 1:3004765/1‑68 (MQ=255) cTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAAATTCGCACTGGCTGGGCACCGGaa < 1:2014047/69‑1 (MQ=255) cTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAAATTCGCACTGGCTGGGCACCGGaa < 1:2328945/69‑1 (MQ=255) cTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAAATTCGCACTGGCTGGGCACCGGaa < 1:2710257/69‑1 (MQ=255) cTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAAATTCGCACTGGCTGGGCACCGGaa < 1:2822652/69‑1 (MQ=255) cTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAAATTCGCACTGGCTGGGCACCGGaa < 1:3189003/69‑1 (MQ=255) cTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAAATTCGCACTGGCTGGGCACCGGaa < 1:947190/69‑1 (MQ=255) gAGGTCGTGAATTCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGt < 1:1769532/69‑1 (MQ=255) gAGGTCGTGAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGt < 1:1263782/69‑1 (MQ=255) gAGGTCGTGAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGt < 1:2158738/69‑1 (MQ=255) aaTGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCg > 1:903014/1‑67 (MQ=255) gCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAagag < 1:3323388/69‑1 (MQ=255) gCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAagag < 1:984660/69‑1 (MQ=255) gCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAagag < 1:2206666/69‑1 (MQ=255) gCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAagag < 1:1463323/69‑1 (MQ=255) gCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAagag < 1:1511223/69‑1 (MQ=255) gCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAagag < 1:2668861/69‑1 (MQ=255) gACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTa > 1:2076187/1‑52 (MQ=255) gACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTa > 1:2054006/1‑52 (MQ=255) gACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTa > 1:353047/1‑52 (MQ=255) cGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCa < 1:198105/69‑1 (MQ=255) gTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCaa > 1:2015761/1‑69 (MQ=255) gTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCaa > 1:2545063/1‑69 (MQ=255) ccTGTTACCTTGAAAATTCGCACTGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTg > 1:2849254/1‑69 (MQ=255) ccTGTTACCCTGAAAATTCGCACTGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACt > 1:403106/1‑68 (MQ=255) ccTGTTACCCTGAAAATTCGCACTGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTg > 1:649785/1‑69 (MQ=255) ccTGTTACCCTGAAAATTCGCACTGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTg > 1:2703435/1‑69 (MQ=255) ccTGTTACCCTGAAAATTCGCACTGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTg > 1:2625563/1‑69 (MQ=255) ccTGTTACCCTGAAAATTCGCACTGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTg > 1:2581243/1‑69 (MQ=255) ccTGTTACCCTGAAAATTCGCACTGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTg > 1:2468346/1‑69 (MQ=255) ccTGTTACCCTGAAAATTCGCACTGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTg > 1:974761/1‑69 (MQ=255) ccTGTTACCCTGAAAATTCGCACTGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTg > 1:2422673/1‑69 (MQ=255) ccTGTTACCCTGAAAATTCGCACTGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTg > 1:2369522/1‑69 (MQ=255) ccTGTTACCCTGAAAATTCGCACTGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTg > 1:1149743/1‑69 (MQ=255) ccTGTTACCCTGAAAATTCGCACTGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTg > 1:1491769/1‑69 (MQ=255) ccTGTTACCCTGAAAATTCGCACTGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTg > 1:2191611/1‑69 (MQ=255) ccTGTTACCCTGAAAATTCGCACTGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTg > 1:2179207/1‑69 (MQ=255) ccTGTTACCCTGAAAATTCGCACTGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTg > 1:2109032/1‑69 (MQ=255) ccTGTTACCCTGAAAATTCGCACTGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTg > 1:186263/1‑69 (MQ=255) ccTGTTACCCTGAAAATTCGCACTGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTg > 1:2030592/1‑69 (MQ=255) aCCCTGAAGATTCGCACCGGCTGGGCACCGGACCACCGTAACTGCGAAGAGATTGCCCAACTGGCTGaa > 1:2854273/1‑69 (MQ=255) aCCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGaa < 1:1121207/69‑1 (MQ=255) aCCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGaa > 1:1321696/1‑69 (MQ=255) aCCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGaa < 1:437720/69‑1 (MQ=255) aCCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGaa < 1:415879/69‑1 (MQ=255) aCCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGaa < 1:1751070/69‑1 (MQ=255) aCCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGaa > 1:1941036/1‑69 (MQ=255) aCCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGaa < 1:2578043/69‑1 (MQ=255) aCCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGaa < 1:2720583/69‑1 (MQ=255) aCCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGaa < 1:2100083/69‑1 (MQ=255) aCCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGaa < 1:2262521/69‑1 (MQ=255) aCCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGaa < 1:2198016/69‑1 (MQ=255) aaaaTTCGCACTGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACtgt > 1:2925548/4‑69 (MQ=255) aaaaTTCGCACTGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACtgt > 1:3072078/4‑69 (MQ=255) aaaaTTCGCACTGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACtgt > 1:3117129/4‑69 (MQ=255) aaaaTTCGCACTGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACtgt > 1:2302871/4‑69 (MQ=255) aaaaTTCGCACTGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACtgt > 1:830527/4‑69 (MQ=255) aaaaTTCGCACTGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACtgt > 1:585689/4‑69 (MQ=255) aaaaTTCGCACTGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACtgt > 1:545267/4‑69 (MQ=255) aaaaTTCGCACTGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACtg > 1:2049962/4‑68 (MQ=255) tCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCGAACTGGCTGAAGACTGTgg > 1:1333839/1‑66 (MQ=255) tCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTgg > 1:1234355/1‑66 (MQ=255) tCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTgg > 1:873979/1‑66 (MQ=255) tCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTgg > 1:941890/1‑66 (MQ=255) tCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTgg > 1:2371679/1‑66 (MQ=255) tCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTgg > 1:2737840/1‑66 (MQ=255) tCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTgg > 1:635264/1‑66 (MQ=255) tCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTgg > 1:480259/1‑66 (MQ=255) tCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTgg > 1:2585007/1‑66 (MQ=255) tCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTgg > 1:1599062/1‑66 (MQ=255) tCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTgg > 1:1671778/1‑66 (MQ=255) tCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTgg > 1:1809136/1‑66 (MQ=255) tCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTgg > 1:1908958/1‑66 (MQ=255) cGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGc < 1:679316/69‑1 (MQ=255) cGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGc < 1:631289/69‑1 (MQ=255) | AAATCGATCCTTACCGAGGTCGTGAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGACTGTGGCATTCAGGC > minE/2225925‑2226055 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |