| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,227,641 | T→C | 58.4% | G238G (GGT→GGC) | yhdY → | predicted amino‑acid transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,227,641 | 0 | T | C | 58.4% | 31.8 / 60.6 | 84 | G238G (GGT→GGC) | yhdY | predicted amino‑acid transporter subunit |
| Reads supporting (aligned to +/- strand): ref base T (20/15); new base C (32/17); total (52/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.99e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AATTGATCCGCGCGCTGGTTGGCGTGATCCTGTTTCAGTCAGCATATGTTGCGGAAGTCGTGCGAGGTGGATTACAGGCGCTGCCTAAAGGGCAATATGAAGCGGCAGAGTCGCTGGCGTTGGGTTACTGGAAA > minE/2227574‑2227707 | aaTTGATCCGCGCGCTGGTTGGCGTGATCCTGTTTCAGTCAGCATATGTTGCGGAAGTCGTGCGAtgtg < 1:2860956/69‑1 (MQ=255)aaTTGATCCGCGCGCTGGTTGGCGTGATCCTGTTTCAGTCAGCATATGTTGCGGAAGTCGTGCGAGGTg < 1:2968674/69‑1 (MQ=255)aaTTGATCCGCGCGCTGGTTGGCGTGATCCTGTTTCAGTCAGCATATGTTGCGGAAGTCGTGCGAGGTg < 1:2892124/69‑1 (MQ=255)aaTTGATCCGCGCGCTGGTTGGCGTGATCCTGTTTCAGTCAGCATATGTTGCGGAAGTCGTGCGAGGTg < 1:2447350/69‑1 (MQ=255)aaTTGATCCGCGCGCTGGTTGGCGTGATCCTGTTTCAGTCAGCATATGTTGCGGAAGTCGTGCGAGGTg < 1:2297972/69‑1 (MQ=255)aaTTGATCCGCGCGCTGGTTGGCGTGATCCTGTTTCAGTCAGCATATGTTGCGGAAGTCGTGCGAGGTg < 1:1337888/69‑1 (MQ=255)aaTTGATCCGCGCGCTGGTTGGCGTGATCCTGTTTCAGTCAGCATATGTTGCGGAAGTCGTGCGAGGTg < 1:1405403/69‑1 (MQ=255)aaTTGATCCGCGCGCTGGTTGGCGTGATCCTGTTTCAGTCAGCATATGTTGCGGAAGGCGTGCGAGGTg < 1:3318951/69‑1 (MQ=255)aaTTGATCCGCGCGCTGGTTGGCGTGATACTGTTTCAGTCAGCATATGTTGCGGAAGTCGTGCGAGGTg < 1:905640/69‑1 (MQ=255) ttGATCCGCGCGCTGGTTGGCGTGATCCTGTTTCAGTCAGCATATGTTGCGGAAGTCGTGCGAGGTgg > 1:1595154/1‑68 (MQ=255) tGATCCTGTTTCAGTCAGCATATGTTGCGGAAGTCGTGCGAGGTg < 1:2095274/45‑1 (MQ=255) ccTGTTTCAGTCAGCATATGTTGCGGAAGTCGTGCGAGGTGGATTACAGGCGCTGCCTAAAGGGCAat > 1:1052251/1‑68 (MQ=255) ccTGTTTCAGTCAGCATATGTTGCGGAAGTCGTGCGAGGTGGATTACAGGCGCTGCCTAAAGGGCAat > 1:1107517/1‑68 (MQ=255) tcagtcagCATATGTTGCGGAAGTCGTGCGAGGCGGAtt > 1:961191/1‑39 (MQ=255) tcagtcagCATATGTTGCGGAAGTCGTGCGAGGCGGATTACAGGCGCTGCCTAAAGGGCAATATGAAg > 1:3233764/1‑68 (MQ=255) tcagtcagCATATGTTGCGGAAGTCGTGCGAGGCGGATTACAGGCGCTGCCTAAAGGGCAATATGAAGc > 1:312116/1‑69 (MQ=255) tcagtcagCATATGTTGCGGAAGTCGTGCGAGGCGGATTACAGGCGCTGCCTAAAGGGCAATATGAAGc > 1:1669431/1‑69 (MQ=255) tcagtcagCATATGTTGCGGAAGTCGTGCGAGGCGGATTACAGGCGCTGCCTAAAGGGCAATATGAAGc > 1:2877754/1‑69 (MQ=255) tcagtcagCATATGTTGCGGAAGTCGTGCGAGGCGGATTACAGGCGCTGCCTAAAGGGCAATATGAAGc > 1:1306758/1‑69 (MQ=255) tcagtcagCATATGTTGCGGAAGTCGTGCGAGGCGGATTACAGGCGCTGCCTAAAGGGCAATATGAAGc > 1:495281/1‑69 (MQ=255) tcagtcagCATATGTTGCGGAAGTCGTGCGAGGCGGATTACAGGCGCTGCCTAAAGGGCAATATGAAGc > 1:455088/1‑69 (MQ=255) tcagtcagCATATGTTGCGGAAGTCGTGAGAGGCGGATTACAGGCGCTGCCTAAAGGGCAATATGAAGc > 1:315535/1‑69 (MQ=255) cagCATATGTTGCGGAAGTCGTGCGAGGCGGATTACAGGCGCTGCCTa < 1:865254/48‑1 (MQ=255) cagCATATGTTGCGGAAGTCGTGCGAGGCGGATTACAGGCGCTGCCTa < 1:1071930/48‑1 (MQ=255) cagCATATGTTGCGGAAGTCGTGCGAGGCGGATTACAGGCGCTGCCTa < 1:968957/48‑1 (MQ=255) cagCATATGTTGCGGAAGTCGTGCGAGGCGGATTACAGGCGCTGCCTa < 1:3174609/48‑1 (MQ=255) cagCATATGTTGCGGAAGTCGTGCGAGGCGGATTACAGGCGCTGCCTa < 1:261576/48‑1 (MQ=255) cagCATATGTTGCGGAAGTCGTGCGAGGCGGATTACAGGCGCTGCCTa < 1:2570270/48‑1 (MQ=255) cagCATATGTTGCGGAAGTCGTGCGAGGCGGATTACAGGCGCTGCCTa < 1:2556563/48‑1 (MQ=255) cagCATATGTTGCGGAAGTCGTGCGAGGCGGATTACAGGCGCTGCCTa < 1:373068/48‑1 (MQ=255) cagCATATGTTGCGGAAGTCGTGCGAGGCGGATTACAGGCGCTGCCTa < 1:1601762/48‑1 (MQ=255) cagCATATGTTGCGGAAGTCGTGCGAGGCGGATTACAGGCGCTGCCTa < 1:1700076/48‑1 (MQ=255) cagCATATGTTGCGGAAGTCGTGCGAGGCGGATTACAGGCGCTGCCTa < 1:560906/48‑1 (MQ=255) cagCATATGTTGCGGAAGTCGTGCGAGGCGGATTACAGGCGCTGCCTa < 1:820692/48‑1 (MQ=255) cagCATATGTTGCGGAAGTCGTGCGAGGCGGATTACAGGCGCTGCCTa < 1:1906702/48‑1 (MQ=255) cagCATATGTTGCGGAAGTCGTGCGAGGCGGATTACAGGCGCTGCCTa < 1:2046102/48‑1 (MQ=255) cagCATATGTTGCGGAAGTCGTGCGAGGCGGATTACAGGCGCTGCCTa < 1:1961790/48‑1 (MQ=255) cagCATATGTTGCGGAAGTCGTGCGAGGCGGATTACAGGCGCTGCCTa < 1:685550/48‑1 (MQ=255) cagCATATGTTGCGGAAGTCGTGCGAGCCGGATTACAGGCGCTGCCTa < 1:524321/48‑1 (MQ=39) agCATATGTTGCGGAAGTCGTGCGAGGTGGATTACAGGCGCTGCCTAAAGGGCAATATGAAGCGGCaga > 1:655194/1‑69 (MQ=255) agCATATGTTGCGGAAGTCGTGCGAGGTGGATTACAGGCGCTGCCTAAAGGGCAATATGAAGCGGCaga > 1:1329748/1‑69 (MQ=255) agCATATGTTGCGGAAGTCGTGCGAGGTGGATTACAGGCGCTGCCTAAAGGGCAATATGAAGCGGCaga > 1:3212869/1‑69 (MQ=255) agCATATGTTGCGGAAGTCGTGCGAGGTGGATTACAGGCGCTGCCTAAAGGGCAATATGAAGCGGCaga > 1:2547371/1‑69 (MQ=255) agCATATGTTGCGGAAGTCGTGCGAGGTGGATTACAGGCGCTGCCTAAAGGGCAATATGAAGCGGCaga > 1:124240/1‑69 (MQ=255) cATATGTTGCGGAAGTCGTGCGAGGCGGATTACAGGCGCTGCCTAAAGGGCAata > 1:478970/1‑55 (MQ=255) cATATGTTGCGGAAGTCGTGCGAGGCGGATTACAGGCGCTGCCTAAAGGGCAata > 1:998270/1‑55 (MQ=255) cATATGTTGCGGAAGTCGTGCGAGGCGGATTACAGGCGCTGCCTAAAGGGCAata > 1:3131585/1‑55 (MQ=255) cATATGTTGCGGAAGTCGTGCGAGGCGGATTACAGGCGCTGCCTAAAGGGCAata > 1:777277/1‑55 (MQ=255) cATATGTTGCGGAAGTCGTGCGAGGCGGATTACAGGCGCTGCCTAAAGGGCAata > 1:3397265/1‑55 (MQ=255) cATATGTTGCGGAAGTCGTGCGAGGCGGATTACAGGCGCTGCCTAAAGGGCAata > 1:3028782/1‑55 (MQ=255) cATATGTTGCGGAAGTCGTGCGAGGCGGATTACAGGCGCTGCCTAAAGGGCAata > 1:919088/1‑55 (MQ=255) cATATGTTGCGGAAGTCGTGCGAGGCGGATTACAGGCGCTGCCTAAAGGGCAata > 1:2536267/1‑55 (MQ=255) cATATGTTGCGGAAGTCGTGCGAGGCGGATTACAGGCGCTGCCTAAAGGGCAata > 1:1381350/1‑55 (MQ=255) cATATGTTGCGGAAGTCGTGCGAGGCGGATTACAGGCGCTGCCTAAAGGGCAata > 1:1709375/1‑55 (MQ=255) cATATGTTGCGGAAGTCGTGCGAGGCGGATTACAGGCGCTGCCTAAAGGGCAata > 1:1879100/1‑55 (MQ=255) cATATGTTGCGGAAGTCGTGCGAGGCGGATTACAGGCGCTGCCTAAAGGGCAata > 1:1929928/1‑55 (MQ=255) cATATGTTGCGGAAGTCGTGCGAGGCGGATTACAGGCGCTGCCTAAAGGGCAata > 1:2202474/1‑55 (MQ=255) cATATGTTGCGGAAGTCGTGCGAGGCGGATTACAGGCGCTGCCTAAAGGGCAata > 1:2263883/1‑55 (MQ=255) cATATGTTGCGGAAGTCGTGCGAGGCGGATTACAGGCGCTGCCTAAAGGGCAata > 1:1089399/1‑55 (MQ=255) cATATGTTGCGGAAGTCGTGCGAGGCGGATTACAGGCGCTGCCTAAAGGGCAata > 1:2520651/1‑55 (MQ=255) cATATGTTGCGGAAGTCGTGCGAGGCGGATTACAGGCGCTGCCTAAAGGGCAata > 1:2547497/1‑55 (MQ=255) cATATGTTGCGGAAGTCGTGCGAGGCGGATTACAGGCGCTGCCTAAAGGGCAata > 1:2622025/1‑55 (MQ=255) cATATGTTGCGGAAGTCGTGCGAGGCGGATTACAGGCGCTGCCTAAAGGGCAata > 1:2960402/1‑55 (MQ=255) cATATGTTGCGGAAGTCGTGCGAGGCGGATTACAGGCGCTGCCTAAAGGGCAata > 1:2927102/1‑55 (MQ=255) cATATGTTGCGGAAGTCGTGCGAGGCGGATTACAGGCGCTGCCTAAAGGGCAata > 1:2626120/1‑55 (MQ=255) cATATGTTGCGGAAGTCGTGCGAGGCGGATTACAGGCGCTGCCTAAAGGGCAata > 1:2671105/1‑55 (MQ=255) cATATGTTGCGGAAGTCGTGCGAGGCGGATTACAGGCGCTGCCTAAAGGGCAat > 1:2462161/1‑54 (MQ=255) tGTTGCGGAAGTCGTGCGAGGTGGATTACAGGCGCTGCCTAAAGGGCAATATGAAGCGGCAGAGTCGc > 1:3300506/1‑68 (MQ=255) tGTTGCGGAAGTCGTGCGAGGTGGATTACAGGCGCTGCCTAAAGGGCAATATGAAGCGGCAGAGTCGc > 1:1604052/1‑68 (MQ=255) tGTTGCGGAAGTCGTGCGAGGTGGATTACAGGCGCTGCCTAAAGGGCAATATGAAGCGGCAGAGTCGc > 1:3037756/1‑68 (MQ=255) tGTTGCGGAAGTCGTGCGAGGTGGATTACAGGCGCTGCCTAAAGGGCAATATGAAGCGGCAGAGTCGc > 1:958134/1‑68 (MQ=255) tGTTGCGGAAGTCGTGCGAGGTGGATTACAGGCGCTGCCTAAAGGGCAATATGAAGCGGCAGAGTCGc > 1:1933610/1‑68 (MQ=255) tGTTGCGGAAGTCGTGCGAGGTGGATTACAGGCGCTGCCTAAAGGGCAATATGAAGCGGCAGAGTCGc > 1:1114364/1‑68 (MQ=255) tGTTGCGGAAGTCGTGCGAGGTGGATTACAGGCGCTGCCTAAAGGGCAATATGAAGCGGCAGAGTCGCt > 1:290988/1‑69 (MQ=255) tGTTGCGGAAGTCGTGCGAGGTGGATTACAGGCGCTGCCTAAAGGGCAATATGAAGCGGCAGAGTCGCt > 1:761015/1‑69 (MQ=255) tGTTGCGGAAGTCGTGCGAGGTGGATTACAGGCGCTGCCTAAAGGGCAATATGAAGCGGCAGAGTCGCt > 1:2546056/1‑69 (MQ=255) aGTCGTGCGAGGTGGATTACAGGCGCTGCCTAAAGGGCAATATGAAGCGGCAGAGTCGCTGGCGTTgg > 1:2785452/1‑68 (MQ=255) aGTCGTGCGAGGTGGATTACAGGCGCTGCCTAAAGGGCAATATGAAGCGGCAGAGTCGCTGGCGTTgg > 1:1041492/1‑68 (MQ=255) aGTCGTGCGAGGTGGATTACAGGCGCTGCCTAAAGGGCAATATGAAGCGGCAGAGTCGCTGGCGTTgg > 1:335485/1‑68 (MQ=255) gTCGTGCGAGGTGGATTAAAGGCGCTGCCTAAAGGGCAATATGAAGCGGCAGAGTCGCTGGCGTTGGGt < 1:2979414/69‑1 (MQ=255) tCGTGCGAGGTGGATTACAGGCGCTGCCTAAAGGGCAATATGAAGCGGCAGAGTCGCTGGCGTTGGGt < 1:3167690/68‑1 (MQ=255) ggTGGATTACAGGGGCTGCCTAAAGGGCAATATGAAGCGGCAGAGTCGCTGGCGTTGGGTTACTGGaaa < 1:1420744/69‑1 (MQ=255) ggTGGATTACAGGCGCTGCCTAAAGGGCAATATGAAGCGGCAGAGTCGCTGGCGTTGGGTTACTGGaaa < 1:758196/69‑1 (MQ=255) ggTGGATTACAGGCGCTGCCTAAAGGGCAATATGAAGCGGCAGAGTCGCTGGCGTTGGGTTACTGGaaa < 1:2200871/69‑1 (MQ=255) ggTGGATTACAGGCGCTGCCTAAAGGGCAATATGAAGCGGCAGAGTCGCTGGCGTTGGGTTACTGGaaa < 1:2359509/69‑1 (MQ=255) | AATTGATCCGCGCGCTGGTTGGCGTGATCCTGTTTCAGTCAGCATATGTTGCGGAAGTCGTGCGAGGTGGATTACAGGCGCTGCCTAAAGGGCAATATGAAGCGGCAGAGTCGCTGGCGTTGGGTTACTGGAAA > minE/2227574‑2227707 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |