| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,228,311 | T→G | 46.2% | V105V (GTT→GTG) | yhdZ → | predicted amino‑acid transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,228,311 | 0 | T | G | 46.2% | 0.3 / 107.3 | 91 | V105V (GTT→GTG) | yhdZ | predicted amino‑acid transporter subunit |
| Reads supporting (aligned to +/- strand): ref base T (33/16); new base G (21/21); total (54/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.34e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01 | |||||||||||
CAGGCAGGAAGTGGGAATGGTCTTTCAGCATTTCAATCTCTTCCCTCATCTGACCGTTTTACAGAACTGTACCCTGGCACCGATTTGGGTACGCAAGATGCCTAAGAAAGAGGCTGAAGATCTG > minE/2228254‑2228377 | caggcaggAAGTGGGAATGGTCTTTCAGCATTTCAATCTCTTCCCTCATCTGACCGTTTTACAGAACTg < 1:940760/69‑1 (MQ=255)caggcaggAAGTGGGAATGGTCTTTCAGCATTTCAATCTCTTCCCTCATCTGACCGTTTTACAGAACTg < 1:1141058/69‑1 (MQ=255)caggcaggAAGTGGGAATGGTCTTTCAGCATTTCAATCTCTTCCCTCATCTGACCGTTTTACAGAACTg < 1:2457881/69‑1 (MQ=255)caggcaggAAGTGGGAATGGTCTTTCAGCATTTCAATCTCTTCCCTCATCTGACCGTTTTACAGAACTg < 1:1006261/69‑1 (MQ=255)caggcaggAAGTGGGAATGGTCTTTCAGCATTTCAATCTCTTCCCTCATCTGACCGTTTTACAGAACTg < 1:867695/69‑1 (MQ=255) aggcaggAAGTGGGAATGGTCTTTCAGCATTTCAATCTCTTCCCTCATCTGACCGTTTTACAGAACTg < 1:224948/68‑1 (MQ=255) aggcaggAAGTGGGAATGGTCTTTCAGCATTTCAATCTCTTCCCTCATCTGACCGTTTTACAGAACTg < 1:286092/68‑1 (MQ=255) gcaggAAGTGGGAATGGTCTTTCAGCATTTCAATCTCTTCCCTCATCTGACCGTTTTACAGAACTGTa < 1:1523424/68‑1 (MQ=255) gcaggAAGTGGGAATGGTCTTTCAGCATTTCAATCTCTTCCCTCATCTGACCGTTTTACAGAACTGTa < 1:2772068/68‑1 (MQ=255) gcaggAAGTGGGAATGGTCTTTCAGCATTTCAATCTCTTCCCTCATCTGACCGTTTTACAGAACTGTa < 1:1312800/68‑1 (MQ=255) gcaggAAGTGGGAATGGTCTTTCAGCATTTCAATCTCTTCCCTCATCTGACCGTTTTACAGAACTGTa < 1:3233007/68‑1 (MQ=255) tGGTCTTTCAGCATTTCAATCTCTTGCCTCATCTGACCGTGTTGCAGAACTGTACCCt < 1:1312922/58‑1 (MQ=255) tGGTCTTTCAGCATTTCAATCTCTTCCCTCATCTGACCGTGTTGCAGAAc > 1:3188751/1‑50 (MQ=39) tGGTCTTTCAGCATTTCAATCTCTTCCCTCATCTGACCGTGTTGCAGAAc > 1:3353534/1‑50 (MQ=39) tGGTCTTTCAGCATTTCAATCTCTTCCCTCATCTGACCGTGTTGCAGAAc > 1:3289174/1‑50 (MQ=39) tGGTCTTTCAGCATTTCAATCTCTTCCCTCATCTGACCGTGTTGCAGAAc > 1:195694/1‑50 (MQ=39) tGGTCTTTCAGCATTTCAATCTCTTCCCTCATCTGACCGTGTTGCAGAAc > 1:511904/1‑50 (MQ=39) tGGTCTTTCAGCATTTCAATCTCTTCCCTCATCTGACCGTGTTGCAGAAc > 1:2875144/1‑50 (MQ=39) tGGTCTTTCAGCATTTCAATCTCTTCCCTCATCTGACCGTGTTGCAGAAc > 1:1982149/1‑50 (MQ=39) tGGTCTTTCAGCATTTCAATCTCTTCCCTCATCTGACCGTGTTGCAGAAc > 1:2864971/1‑50 (MQ=39) tGGTCTTTCAGCATTTCAATCTCTTCCCTCATCTGACCGTGTTGCAGAAc > 1:2210658/1‑50 (MQ=39) tGGTCTTTCAGCATTTCAATCTCTTCCCTCATCTGACCGTGTTGCAGAAc > 1:253804/1‑50 (MQ=39) tGGTCTTTCAGCATTTCAATCTCTTCCCTCATCTGACCGTGTTGCAGAAc > 1:2431135/1‑50 (MQ=39) tGGTCTTTCAGCATTTCAATCTCTTCCCTCATCTGACCGTGTTGCAGAAc > 1:789329/1‑50 (MQ=39) tGGTCTTTCAGCATTTCAATCTCTTCCCTCATCTGACCGTGTTGCAGAAc > 1:1119568/1‑50 (MQ=39) tGGTCTTTCAGCATTTCAATCTCTTCCCTCATCTGACCGTGTTGCAGAAc > 1:940258/1‑50 (MQ=39) tGGTCTTTCAGCATTTCAATCTCTTCCCTCATCTGACCGTGTTGCAGAAc > 1:905731/1‑50 (MQ=39) tGGTCTTTCAGCATTTCAATCTCTTCCCTCATCTGACCGTGTTGCAGAAc > 1:727644/1‑50 (MQ=39) tGGTCTTTCAGCATTTCAATCTCTTCCCTCATCTGACCGTGTTGCAGAAc > 1:608905/1‑50 (MQ=39) tGGTCTTTCAGCATTTCAATCTCTTCCCTCATCTGACCGTGTTGCAGAAc > 1:585570/1‑50 (MQ=39) tGGTCTTTCAGCATTTCAATCTCTTCCCTCATCTGACCGTGTTGCAGAAc > 1:533484/1‑50 (MQ=39) tGGTCTTTCAGCATTTCAATCTCTTCCCTCATCTGACCGTGTTGCAGAAc > 1:46701/1‑50 (MQ=39) tGGTCTTTCAGCATTTCAATCTCTTCCCTCATCTGACCGTGTTGCAGAAc > 1:461397/1‑50 (MQ=39) tGGTCTTTCAGCATTTCAATCTCTTCCCTCATCTGACCGTGTTGCAGAACTGTACCCt < 1:561957/58‑1 (MQ=255) tGGTCTTTCAGCATTTCAATCTCTTCCCTCATCTGACCGTGTTGCAGAACTGTACCCt < 1:3361791/58‑1 (MQ=255) tGGTCTTTCAGCATTTCAATCTCTTCCCTCATCTGACCGTGTTGCAGAACTGTACCCt < 1:808826/58‑1 (MQ=255) tGGTCTTTCAGCATTTCAATCTCTTCCCTCATCTGACCGTGTTGCAGAACTGTACCCt < 1:891791/58‑1 (MQ=255) tGGTCTTTCAGCATTTCAATCTCTTCCCTCATCTGACCGTGTTGCAGAACTGTACCCt < 1:903068/58‑1 (MQ=255) tGGTCTTTCAGCATTTCAATCTCTTCCCTCATCTGACCGTGTTGCAGAACTGTACCCt < 1:2573890/58‑1 (MQ=255) tGGTCTTTCAGCATTTCAATCTCTTCCCTCATCTGACCGTGTTGCAGAACTGTACCCt < 1:930498/58‑1 (MQ=255) tGGTCTTTCAGCATTTCAATCTCTTCCCTCATCTGACCGTGTTGCAGAACTGTACCCt < 1:2526380/58‑1 (MQ=255) tGGTCTTTCAGCATTTCAATCTCTTCCCTCATCTGACCGTGTTGCAGAACTGTACCCt < 1:1968195/58‑1 (MQ=255) tGGTCTTTCAGCATTTCAATCTCTTCCCTCATCTGACCGTGTTGCAGAACTGTACCCt < 1:1364360/58‑1 (MQ=255) tGGTCTTTCAGCATTTCAATCTCTTCCCTCATCTGACCGTGTTGCAGAACTGTACCCt < 1:1404609/58‑1 (MQ=255) tGGTCTTTCAGCATTTCAATCTCTTCCCTCATCTGACCGTGTTGCAGAACTGTACCCt < 1:1425603/58‑1 (MQ=255) tGGTCTTTCAGCATTTCAATCTCTTCCCTCATCTGACCGTGTTGCAGAACTGTACCCt < 1:1361616/58‑1 (MQ=255) tGGTCTTTCAGCATTTCAATCTCTTCCCTCATCTGACCGTGTTGCAGAACTGTACCCt < 1:1257584/58‑1 (MQ=255) tGGTCTTTCAGCATTTCAATCTCTTCCCTCATCTGACCGTGTTGCAGAACTGTACCCt < 1:137658/58‑1 (MQ=255) tGGTCTTTCAGCATTTCAATCTCTTCCCTCATCTGACCGTGTTGCAGAACTGTACCCt < 1:1215792/58‑1 (MQ=255) tGGTCTTTCAGCATTTCAATCTCTTCCCTCATCTGACCGTGTTGCAGAACTGTACCCt < 1:222793/58‑1 (MQ=255) tGGTCTTTCAGCATTTCAATCTCTTCCCTCATCTGACCGTGTTGCAGAACTGTACCCt < 1:2341450/58‑1 (MQ=255) tGGTCTTTCAGCATTTCAATCTCTTCCCTCATCTGACCGTGTTGCAGAACTGTACCCt < 1:1162825/58‑1 (MQ=255) gTCTTTCAGCATTTCAATCTCTTCCCTCATCTGACCGTTTTACAGAACTGTACCCTGGCACCGATTTg > 1:1935106/1‑68 (MQ=255) gTCTTTCAGCATTTCAATCTCTTCCCTCATCTGACCGTTTTACAGAACTGTACCCTGGCACCGATTTg > 1:1245779/1‑68 (MQ=255) aGCATTTCAATCTCTTCCCTCATCTGACCGTGTTGCAGAACTGTACCCt < 1:45147/49‑1 (MQ=38) cATTTCAATCTCTTCCCTCATCTGACCGTTTTACAGAACTGTACCCTGGCACCGATTTGGGTACGCaa < 1:1169758/68‑1 (MQ=255) cATTTCAATCTCTTCCCTCATCTGACCGTTTTACAGAACTGTACCCTGGCACCGATTTGGGTACGCaa < 1:738226/68‑1 (MQ=255) tCAATCTCTTACCTCATCTGACCGTTTTACAGAACTGTa > 1:918194/1‑39 (MQ=255) tctcTTCCCTCATCTGACCGTTTTACAGAACTGTACCCTGGCACCGATTTGGGTa > 1:1695351/1‑55 (MQ=255) tctcTTCCCTCATCTGACCGTTTTACAGAACTGTACCCTGGCACCGATTTGGGTACGCAAGATGCCTa > 1:2441862/1‑68 (MQ=255) tctcTTCCCTCATCTGACCGTTTTACAGAACTGTACCCTGGCACCGATTTGGGTACGCAAGATGCCTa > 1:2775423/1‑68 (MQ=255) tctcTTCCCTCATCTGACCGTTTTACAGAACTGTACCCTGGCACCGATTTGGGTACGCAAGATGCCTa > 1:485890/1‑68 (MQ=255) tctcTTCCCTCATCTGACCGTTTTACAGAACTGTACCCTGGCACCGATTTGGGTACGCAAGATGCCTa > 1:3376214/1‑68 (MQ=255) tctcTTCCCTCATCTGACCGTTTTACAGAACTGTACCCTGGCACCGATTTGGGTACGCAAGATGCCTa > 1:336075/1‑68 (MQ=255) tctcTTCCCTCATCTGACCGTTTTACAGAACTGTACCCTGGCACCGATTTGGGTACGCAAGATGCCTa > 1:3158301/1‑68 (MQ=255) ctctTCCCTCATCTGCCCGTTTTACAGAACTGTACCCTGGCACCGATTTGGGTACGCa > 1:2307064/1‑58 (MQ=255) ctctTCCCTCATCTGACCGTTTTACAGAACTGTACCCTGGCACCGATTTGGGTACGCa > 1:1943257/1‑58 (MQ=255) ctctTCCCTCATCTGACCGTTTTACAGAACTGTACCCTGGCACCGATTTGGGTACGCa > 1:3295644/1‑58 (MQ=255) ctctTCCCTCATCTGACCGTTTTACAGAACTGTACCCTGGCACCGATTTGGGTACGCa > 1:3229696/1‑58 (MQ=255) ctctTCCCTCATCTGACCGTTTTACAGAACTGTACCCTGGCACCGATTTGGGTACGCa > 1:1968717/1‑58 (MQ=255) ctctTCCCTCATCTGACCGTTTTACAGAACTGTACCCTGGCACCGATTTGGGTACGCa > 1:493372/1‑58 (MQ=255) ctctTCCCTCATCTGACCGTTTTACAGAACTGTACCCTGGCACCGATTTGGGTACGCa > 1:3109694/1‑58 (MQ=255) ctctTCCCTCATCTGACCGTTTTACAGAACTGTACCCTGGCACCGATTTGGGTACGCa > 1:2554986/1‑58 (MQ=255) ctctTCCCTCATCTGACCGTTTTACAGAACTGTACCCTGGCACCGATTTGGGTACGCa > 1:144253/1‑58 (MQ=255) ctctTCCCTCATCTGACCGTTTTACAGAACTGTACCCTGGCACCGATTTGGGTACGCa > 1:2984072/1‑58 (MQ=255) ctctTCCCTCATCTGACCGTTTTACAGAACTGTACCCTGGCACCGATTTGGGTACGCa > 1:1132157/1‑58 (MQ=255) ctctTCCCTCATCTGACCGTTTTACAGAACTGTACCCTGGCACCGATTTGGGTACGCa > 1:2945362/1‑58 (MQ=255) ctctTCCCTCATCTGACCGTTTTACAGAACTGTACCCTGGCACCGATTTGGGTACGCa > 1:1969744/1‑58 (MQ=255) ctctTCCCTCATCTGACCGTTTTACAGAACTGTACCCTGGCACCGATTTGGGTACGCAAGATGCCTaa > 1:800257/1‑68 (MQ=255) cATCTGACCGTTTTACAAAACTGTACCCTGGCACCGATTTGGGTACGCaa < 1:1962466/50‑1 (MQ=255) tGACCGTTTTACAGAACTGTACCCTGGCACCGATTTGGGTACGCAAGATGCCTAAGAAAGAgg > 1:682746/1‑63 (MQ=255) tGACCGTTTTACAGAACTGTACCCTGGCACCGATTTGGGTACGCAAGATGCCTAAGAAAGAGGc > 1:95341/1‑64 (MQ=255) tGACCGTTTTACAGAACTGTACCCTGGCACCGATTTGGGTACGCAAGATGCCTAAGAAAGAGGc > 1:1386983/1‑64 (MQ=255) tGACCGTTTTACAGAACTGTACCCTGGCACCGATTTGGGTACGCAAGATGCCTAAGAAAGAGGc > 1:1936502/1‑64 (MQ=255) tGACCGTTTTACAGAACTGTACCCTGGCACCGATTTGGGTACGCAAGATGCCTAAGAAAGAGGc > 1:2089788/1‑64 (MQ=255) tGACCGTTTTACAGAACTGTACCCTGGCACCGATTTGGGTACGCAAGATGCCTAAGAAAGAGGc > 1:2284956/1‑64 (MQ=255) tGACCGTTTTACAGAACTGTACCCTGGCACCGATTTGGGTACGCAAGATGCCTAAGAAAGAGGc > 1:2730974/1‑64 (MQ=255) tGACCGTTTTACAGAACTGTACCCTGGCACCGATTTGGGTACGCAAGATGCCTAAGAAAGAGGc > 1:2341564/1‑64 (MQ=255) tGACAGTTTTACAGAACTGTACCCTGGCACCGATTTGGGTACGCAAGATGCCTaagaaaga > 1:1688980/1‑61 (MQ=255) gTTTTACAGAACTGTACCCTGGCACCGATTTGGGTACGCAAGATGCCTAAGAAAGAGGCTGAAGATCTg < 1:1845244/69‑1 (MQ=255) gTTTTACAGAACTGTACCCTGGCACCGATTTGGGTACGCAAGATGCCTAAGAAAGAGGCTGAAGATCTg < 1:2647626/69‑1 (MQ=255) ttttACAGAACTGTACCCTGGCACCGATTTGGGTACGCAAGATGCCTAAGAAAGAGGCTGAAGATCTg < 1:2347472/68‑1 (MQ=255) | CAGGCAGGAAGTGGGAATGGTCTTTCAGCATTTCAATCTCTTCCCTCATCTGACCGTTTTACAGAACTGTACCCTGGCACCGATTTGGGTACGCAAGATGCCTAAGAAAGAGGCTGAAGATCTG > minE/2228254‑2228377 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |