| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,228,356 | T→A | 30.5% | P120P (CCT→CCA) | yhdZ → | predicted amino‑acid transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,228,356 | 0 | T | A | 30.5% | 96.8 / 54.2 | 82 | P120P (CCT→CCA) | yhdZ | predicted amino‑acid transporter subunit |
| Reads supporting (aligned to +/- strand): ref base T (40/17); new base A (11/14); total (51/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.91e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCTCTTCCCTCATCTGACCGTTTTACAGAACTGTACCCTGGCACCGATTTGGGTACGCAAGATGCCTAAGAAAGAGGCTGAAGATCTGGCGGTGCATTACCTAGAGCGGGTGAGAATTGCCGAACATGCG > minE/2228290‑2228419 | tctcTTCCCTCATCTGACCGTTTTACAGAACTGTACCCTGGCACCGATTTGGGTACGCAAGATGCCTa > 1:2441862/1‑68 (MQ=255)tctcTTCCCTCATCTGACCGTTTTACAGAACTGTACCCTGGCACCGATTTGGGTACGCAAGATGCCTa > 1:3376214/1‑68 (MQ=255)tctcTTCCCTCATCTGACCGTTTTACAGAACTGTACCCTGGCACCGATTTGGGTACGCAAGATGCCTa > 1:485890/1‑68 (MQ=255)tctcTTCCCTCATCTGACCGTTTTACAGAACTGTACCCTGGCACCGATTTGGGTACGCAAGATGCCTa > 1:2775423/1‑68 (MQ=255)tctcTTCCCTCATCTGACCGTTTTACAGAACTGTACCCTGGCACCGATTTGGGTACGCAAGATGCCTa > 1:336075/1‑68 (MQ=255)tctcTTCCCTCATCTGACCGTTTTACAGAACTGTACCCTGGCACCGATTTGGGTACGCAAGATGCCTa > 1:3158301/1‑68 (MQ=255) ctctTCCCTCATCTGACCGTTTTACAGAACTGTACCCTGGCACCGATTTGGGTACGCAAGATGCCTaa > 1:800257/1‑68 (MQ=255) tGACCGTTTTACAGAACTGTACCCTGGCACCGATTTGGGTACGCAAGATGCCTAAGAAAGAgg > 1:682746/1‑63 (MQ=255) tGACCGTTTTACAGAACTGTACCCTGGCACCGATTTGGGTACGCAAGATGCCTAAGAAAGAGGc > 1:2284956/1‑64 (MQ=255) tGACCGTTTTACAGAACTGTACCCTGGCACCGATTTGGGTACGCAAGATGCCTAAGAAAGAGGc > 1:2730974/1‑64 (MQ=255) tGACCGTTTTACAGAACTGTACCCTGGCACCGATTTGGGTACGCAAGATGCCTAAGAAAGAGGc > 1:1386983/1‑64 (MQ=255) tGACCGTTTTACAGAACTGTACCCTGGCACCGATTTGGGTACGCAAGATGCCTAAGAAAGAGGc > 1:2089788/1‑64 (MQ=255) tGACCGTTTTACAGAACTGTACCCTGGCACCGATTTGGGTACGCAAGATGCCTAAGAAAGAGGc > 1:2341564/1‑64 (MQ=255) tGACCGTTTTACAGAACTGTACCCTGGCACCGATTTGGGTACGCAAGATGCCTAAGAAAGAGGc > 1:95341/1‑64 (MQ=255) tGACCGTTTTACAGAACTGTACCCTGGCACCGATTTGGGTACGCAAGATGCCTAAGAAAGAGGc > 1:1936502/1‑64 (MQ=255) tGACAGTTTTACAGAACTGTACCCTGGCACCGATTTGGGTACGCAAGATGCCTaagaaaga > 1:1688980/1‑61 (MQ=255) gTTTTACAGAACTGTACCCTGGCACCGATTTGGGTACGCAAGATGCCTAAGAAAGAGGCTGAAGATCTg < 1:1845244/69‑1 (MQ=255) gTTTTACAGAACTGTACCCTGGCACCGATTTGGGTACGCAAGATGCCTAAGAAAGAGGCTGAAGATCTg < 1:2647626/69‑1 (MQ=255) ttttACAGAACTGTACCCTGGCACCGATTTGGGTACGCAAGATGCCTAAGAAAGAGGCTGAAGATCTg < 1:2347472/68‑1 (MQ=255) aGAACTGTACCCTGGCACCGATTTGGGTACGCAAGATGCCTAAGAAAGAGGCTGAAGATCTGGCGGTg < 1:2294239/68‑1 (MQ=255) aGAACTGTACCCTGGCACCGATTTGGGTACGCAAGATGCCTAAGAAAGAGGCTGAAGATCTGGCGGTg < 1:861704/68‑1 (MQ=255) aGAACTGTACCCTGGCACCGATTTGGGTACGCAAGATGCCTAAGAAAGAGGCTGAAGATCTGGCGGTg < 1:951520/68‑1 (MQ=255) aGAACTGTACCCTGGCACCGATTTGGGTACGCAAGATGCCTAAGAAAGAGGCTGAAGATCTGGCGGTg < 1:2206767/68‑1 (MQ=255) gAACTGTACCCTGGCACCGATTTGGGTACGCAAGATGCCTAAGAAAGAGGCTGAAGATCTGGCGGTg < 1:2878610/67‑1 (MQ=255) aCTGTACCCTGGCACCGATTTGGGTACGCAAGATGCCTAAGAAAGAGGCTGAAGATCTGGCGGTGCAtt > 1:1686684/1‑69 (MQ=255) aCTGTACCCTGGCACCGATTTGGGTACGCAAGATGCCTAAGAAAGAGGCTGAAGATCTGGCGGTGCAtt > 1:1924668/1‑69 (MQ=255) aCTGTACCCTGGCACCGATTTGGGTACGCAAGATGCCTAAGAAAGAGGCTGAAGATCTGGCGGTGCAtt > 1:1078743/1‑69 (MQ=255) tGGCACCGATTTGGGTACGCAAGATGCCAAAGAAAGAGGCTGAAgct > 1:2547114/1‑45 (MQ=255) tGGCACCGATTTGGGTACGCAAGATGCCAAAGAAAGAGGCTGAAgct > 1:2633750/1‑45 (MQ=255) tGGCACCGATTTGGGTACGCAAGATGCCAAAGAAAGAGGCTGAAgct > 1:2623277/1‑45 (MQ=255) tGGCACCGATTTGGGTACGCAAGATGCCAAAGAAAGAGGCTGAAgct > 1:2214022/1‑45 (MQ=255) tGGCACCGATTTGGGTACGCAAGATGCCAAAGAAAGAGGCTGAAgct > 1:3036557/1‑45 (MQ=255) tGGCACCGATTTGGGTACGCAAGATGCCAAAGAAAGAGGCTGAAgct > 1:3275081/1‑45 (MQ=255) tGGCACCGATTTGGGTACGCAAGATGCCAAAGAAAGAGGCTGAAgct > 1:1758294/1‑45 (MQ=255) tGGCACCGATTTGGGTACGCAAGATGCCAAAGAAAGAGGCTGAAgct > 1:545442/1‑45 (MQ=255) tGGCACCGATTTGGGTACGCAAGATGCCAAAGAAAGAGGCTGAAgct > 1:1403309/1‑45 (MQ=255) tGGCACCGATTTGGGTACGCAAGATGCCAAAGAAAGAGGCTGAAgct > 1:1312107/1‑45 (MQ=255) tGGCACCGATTTGGGTACGCAAGATGCCAAAGAAAGAGGCTGAAgct > 1:1285205/1‑45 (MQ=255) aTTTGGGTACGCAAGATGCCTAAGAAAGAGGCTGAAGATCTGGTGGTGCATTACCTAGAGCGGGTgaga < 1:1000544/69‑1 (MQ=255) aTTTGGGTACGCAAGATGCCTAAGAAAGAGGCTGAAGATCTGGCGGTGCATTACCTAGAGCGGGTgaga < 1:743582/69‑1 (MQ=255) aTTTGGGTACGCAAGATGCCTAAGAAAGAGGCTGAAGATCTGGCGGTGCATTACCTAGAGCGGGTgaga < 1:957485/69‑1 (MQ=255) aTTTGGGTACGCAAGATGCCTAAGAAAGAGGCTGAAGATCTGGCGGTGCATTACCTAGAGCGGGTgaga < 1:1031483/69‑1 (MQ=255) aTTTGGGTACGCAAGATGCCTAAGAAAGAGGCTGAAGATCTGGCGGTGCATTACCTAGAGCGGGTgaga < 1:1807893/69‑1 (MQ=255) aTTTGGGTACGCAAGATGCCTAAGAAAGAGGCTGAAGATCTGGCGGTGCATTACCTAGAGCGGGTgaga < 1:1273898/69‑1 (MQ=255) aTTTGGGTACGCAAGATGCCTAAGAAAGAGGCTGAAGATCTGGCGGTGCATTACCTAGAGCGGGTgaga < 1:1913922/69‑1 (MQ=255) aTTTGGGTACGCAAGATGCCTAAGAAAGAGGCTGAAGATCTGGCGGTGCATTACCTAGAGCGGGTgaga < 1:1670333/69‑1 (MQ=255) tttGGGTACGCAAGATGCCTAAGAAAGAGGCTGAAGATCTGGCGGTGCATTACCTAGAGCGGGTgaga < 1:3258382/68‑1 (MQ=255) gTACGCAAGATGCCAAAGAAAGAGGCTGAAGCTCTGGCGATGCATTACCTTGAGCGGGTGAGAATTGc < 1:2041646/68‑1 (MQ=255) gTACGCAAGATGCCAAAGAAAGAGGCTGAAGCTCTGGCGATGCATTACCTTGAGCGGGTGAGAATTGc < 1:798057/68‑1 (MQ=255) gTACGCAAGATGCCAAAGAAAGAGGCTGAAGCTCTGGCGATGCATTACCTTGAGCGGGTGAGAATTGc < 1:32578/68‑1 (MQ=255) gTACGCAAGATGCCAAAGAAAGAGGCTGAAGCTCTGGCGATGCATTACCTTGAGCGGGTGAGAATTGc < 1:754133/68‑1 (MQ=255) gTACGCAAGATGCCAAAGAAAGAGGCTGAAGCTCTGGCGATGCATTACCTTGAGCGGGTGAGAATTGc < 1:67291/68‑1 (MQ=255) gTACGCAAGATGCCAAAGAAAGAGGCTGAAGCTCTGGCGATGCATTACCTTGAGCGGGTGAGAATTGc < 1:89354/68‑1 (MQ=255) gTACGCAAGATGCCAAAGAAAGAGGCTGAAGCTCTGGCGATGCATTACCTTGAGCGGGTGAGAATTGc < 1:1166599/68‑1 (MQ=255) gTACGCAAGATGCCAAAGAAAGAGGCTGAAGCTCTGGCGATGCATTACCTTGAGCGGGTGAGAATTGc < 1:1049471/68‑1 (MQ=255) gTACGCAAGATGCCAAAGAAAGAGGCTGAAGCTCTGGCGATGCATTACCTTGAGCGGGTGAGAATTGc < 1:2274344/68‑1 (MQ=255) gTACGCAAGATGCCAAAGAAAGAGGCTGAAGCTCTGGCGATGCATTACCTTGAGCGGGTGAGAATTGc < 1:2361697/68‑1 (MQ=255) gTACGCAAGATGCCAAAGAAAGAGGCTGAAGCTCTGGCGATGCATTACCTTGAGCGGGTGAGAATTGc < 1:1036753/68‑1 (MQ=255) gTACGCAAGATGCCAAAGAAAGAGGCTGAAGCTCTGGCGATGCATTACCTTGAGCGGGTGAGAATTGc < 1:960024/68‑1 (MQ=255) gTACGCAAGATGCCAAAGAAAGAGGCTGAAGCTCTGGCGATGCATTACCTTGAGCGGGTGAGAATTGc < 1:967604/68‑1 (MQ=255) gTACGCAAGATGCCAAAGAAAGAGGCTGAAGCTCTGGCGATGCATTACCTTGAGCCGGTGAGAATTGc < 1:3081543/68‑1 (MQ=255) cAAGATGCCTAAGAAAGAGGCTGAAGATCTGGCGGTGCATTACCTAGAGCGGGTGAGAATTGCCGAACa > 1:3094428/1‑69 (MQ=255) aTGCCTAAGAAAGAGGCTGAAGATCTGGCGGTGCAtt > 1:2535593/1‑37 (MQ=255) aTGCCTAAGAAAGAGGCTGAAGATCTGGCGGTGCAtt > 1:855385/1‑37 (MQ=255) aTGCCTAAGAAAGAGGCTGAAGATCTGGCGGTGCAtt > 1:517665/1‑37 (MQ=255) aTGCCTAAGAAAGAGGCTGAAGATCTGGCGGTGCAtt > 1:482453/1‑37 (MQ=255) aTGCCTAAGAAAGAGGCTGAAGATCTGGCGGTGCAtt > 1:2601813/1‑37 (MQ=255) aTGCCTAAGAAAGAGGCTGAAGATCTGGCGGTGCAtt > 1:253421/1‑37 (MQ=255) aTGCCTAAGAAAGAGGCTGAAGATCTGGCGGTGCAtt > 1:2478674/1‑37 (MQ=255) aTGCCTAAGAAAGAGGCTGAAGATCTGGCGGTGCAtt > 1:2866763/1‑37 (MQ=255) aTGCCTAAGAAAGAGGCTGAAGATCTGGCGGTGCAtt > 1:2224396/1‑37 (MQ=255) aTGCCTAAGAAAGAGGCTGAAGATCTGGCGGTGCAtt > 1:1757231/1‑37 (MQ=255) aTGCCTAAGAAAGAGGCTGAAGATCTGGCGGTGCAtt > 1:1716904/1‑37 (MQ=255) aTGCCTAAGAAAGAGGCTGAAGATCTGGCGGTGCAt > 1:3265285/1‑36 (MQ=255) aTGCATAAGAAAGAGGCTGAAGATCTGGCGGTGCAtt > 1:1458493/1‑37 (MQ=255) tGCCTAAGAAAGAGGCTGAAGATCTGGCGGTGCATTACCTAGAGCGGGTGAGAATTGCCGAACATgcg > 1:1859215/1‑68 (MQ=255) tGCCTAAGAAAGAGGCTGAAGATCTGGCGGTGCATTACCTAGAGCGGGTGAGAATTGCCGAACATgcg > 1:802298/1‑68 (MQ=255) tGCCTAAGAAAGAGGCTGAAGATCTGGCGGTGCATTACCTAGAGCGGGTGAGAATTGCCGAACATgcg > 1:305751/1‑68 (MQ=255) tGCCTAAGAAAGAGGCTGAAGATCTGGCGGTGCATTACCTAGAGCGGGTGAGAATTGCCGAACATgcg > 1:123577/1‑68 (MQ=255) tGCCTAAGAAAGAGGCTGAAGATCTGGCGGTGCATTACCTAGAGCGGGTGAGAATTGCCGAACATgcg > 1:1317290/1‑68 (MQ=255) tGCCTAAGAAAGAGGCTGAAGATCTGGCGGTGCATTACCTAGAGCGGGTGAGAATTGCCGAACATgcg > 1:2798271/1‑68 (MQ=255) tGCCTAAGAAAGAGGCTGAAGATCTGGCGGTGCATTACCTAGAGCGGGTGAGAATTGCCGAACATgcg > 1:3385526/1‑68 (MQ=255) | TCTCTTCCCTCATCTGACCGTTTTACAGAACTGTACCCTGGCACCGATTTGGGTACGCAAGATGCCTAAGAAAGAGGCTGAAGATCTGGCGGTGCATTACCTAGAGCGGGTGAGAATTGCCGAACATGCG > minE/2228290‑2228419 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |