| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,237,409 | A→G | 40.0% | K318K (AAA→AAG) | purD → | phosphoribosylglycinamide synthetase phosphoribosylamine‑glycine ligase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,237,409 | 0 | A | G | 40.0% | 43.4 / 74.5 | 105 | K318K (AAA→AAG) | purD | phosphoribosylglycinamide synthetase phosphoribosylamine‑glycine ligase |
| Reads supporting (aligned to +/- strand): ref base A (39/24); new base G (13/29); total (52/53) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.69e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01 | |||||||||||
Only 100 of 108 total aligned reads displayed.
AACCCAGCCGATTATGCTGCGCATGAAGTCCGATCTGGTTGAGCTCTGCCTGGCGGCCTGTGAAAGCAAACTGGACGAGAAAACGTCCGAGTGGGATGAACGCGCTTCTCTCGGCGTGGTGATGGCTGCGGGTGGA > minE/2237340‑2237475 | aaCCCAGCCGATTATGCTGCGCATGAAGTCCGATCTGGTTGAGCT‑TGCCTGGCGGCCTGTGAAAGCaaa > 1:3219507/1‑69 (MQ=255) ccAGCCGATTATGCTGCGCATGAAGTCCGATCTGGTTGAGCTCTGCCTGGCGGCCTGTGAAAGCAAAc > 1:11921/1‑68 (MQ=255) ccAGCCGATTATGCTGCGCATGAAGTCCGATCTGGTTGAGCTCTGCCTGGCGGCCTGTGAAAGCAAAc > 1:265700/1‑68 (MQ=255) ccAGCCGATTATGCTGCGCATGAAGTCCGATCTGGTTGAGCTCTGCCTGGCGGCCTGTGAAAGCAAAc > 1:139084/1‑68 (MQ=255) cAGCCGATTATGCTGCGCATGAAGTCCGATCTGGTTGAGCTCTGCCTGGCGGCCTGTGAAAGCAAACTg > 1:955298/1‑69 (MQ=255) cAGCCGATTATGCTGCGCATGAAGTCCGATCTGGTTGAGCTCTGCCTGGCGGCCTGTGAAAGCAAACTg > 1:559786/1‑69 (MQ=255) cAGCCGATTATGCTGCGCATGAAGTCCGATCTGGTTGAGCTCTGCCTGGCGGCCTGTGAAAGCAAACTg > 1:2934985/1‑69 (MQ=255) cAGCCGATTATGCTGCGCATGAAGTCCGATCTGGTTGAGCTCTGCCTGGCGGCCTGTGAAAGCAAACTg > 1:725168/1‑69 (MQ=255) cAGCCGATTATGCTGCGCATGAAGTCCGATCTGGTTGAGCTCTGCCTGGCGGCCTGTGAAAGCAAACTg > 1:2448942/1‑69 (MQ=255) cGATTATGCTGCGCATGAAGTCCGATCTGGTTGAGCTCTGCCTGGCGGCCTGTGAAGGCAAGCTgg > 1:535009/1‑66 (MQ=255) cGATTATGCTGCGCATGAAGTCCGATCTGGTTGAGCTCTGCCTGGCGGCCTGTGAAGGCAAGCTgg > 1:2220613/1‑66 (MQ=255) cGATTATGCTGCGCATGAAGTCCGATCTGGTTGAGCTCTGCCTGGCGGCCTGTGAAGGCAAGCTgg > 1:595949/1‑66 (MQ=255) cGATTATGCTGCGCATGAAGTCCGATCTGGTTGAGCTCTGCCTGGCGGCCTGTGAAGGCAAGCTgg > 1:1611180/1‑66 (MQ=255) cGATTATGCTGCGCATGAAGTCCGATCTGGTTGAGCTCTGCCTGGCGGCCTGTGAAGGCAAGCTgg > 1:1441341/1‑66 (MQ=255) cGATTATGCTGCGCATGAAGTCCGATCTGGTTGAGCTCTGCCTGGCGGCCTGTGAAGGCAAGCTgg > 1:627140/1‑66 (MQ=255) cGATTATGCTGCGCATGAAGTCCGATCTGGTTGAGCTCTGCCTGGCGGCCTGTGAAGGCAAGCTgg > 1:1649603/1‑66 (MQ=255) cGATTATGCTGCGCATGAAGTCCGATCTGGTTGAGCTCTGCCTGGCGGCCTGTGAAGGCAAGCTgg > 1:1163355/1‑66 (MQ=255) cGATTATGCTGCGCATGAAGTCCGATCTGGTTGAGCTCTGCCTGGCGGCCTGTGAAGGCAAGCTgg > 1:494553/1‑66 (MQ=255) cGATTATGCTGCGCATGAAGTCCGATCTGGTTGAGCTCTGCCTGGCGGCCTGTGAAGGCAAGCTgg > 1:860796/1‑66 (MQ=255) cGATTATGCTGCGCATGAAGTCCGATCTGGTTGAGCTCTGCCTGGCGGCCTGTGAAGGCAAGCTg > 1:1261923/1‑65 (MQ=255) gATTATGCTGCGCATGAAGTCCGATCTGGTTGAGCTCTGCCTGGCGGCCTGTGAAAGCAAACTGGACg > 1:1847876/1‑68 (MQ=255) aTGCTGCGCATGAAGTCCGATCTGGTTGAGCTCTGCCTGGCGGCCTGTGAAAGCAAACTGGACGAGaaa > 1:2725098/1‑69 (MQ=255) aTGCTGCGCATGAAGTCCGATCTGGTTGAGCTCTGCCTGGCGGCCTGTGAAAGCAAACTGGACGAGaaa > 1:2132314/1‑69 (MQ=255) cATGAAGTCCGATCTGGTTGAGCTCTGCCTGGCGGCCTGTGAAAGCAAACTGGACGAGAAAACGTCCGa > 1:3390681/1‑69 (MQ=255) cGATCTGGTTGAGCTCTGCCTGGCGGCCTGTGAAAGCAAACTGGACGAGAAAACGTCCGAGTgg > 1:532309/1‑64 (MQ=255) cGATCTGGTTGAGCTCTGCCTGGCGGCCTGTGAAAGCAAACTGGACGAGAAAACGTCCGAGTgg > 1:513757/1‑64 (MQ=255) cGATCTGGTTGAGCTCTGCCTGGCGGCCTGTGAAAGCAAACTGGACGAGAAAACGTCCGAGTgg > 1:3174990/1‑64 (MQ=255) cGATCTGGTTGAGCTCTGCCTGGCGGCCTGTGAAAGCAAACTGGACGAGAAAACGTCCGAGTgg > 1:3273545/1‑64 (MQ=255) cGATCTGGTTGAGCTCTGCCTGGCGGCCTGTGAAAGCAAACTGGACGAGAAAACGTCCGAGTgg > 1:2529174/1‑64 (MQ=255) ctctGCCTGGCGGCCTGTGAAGGCAAGCTGGACGAGAAAACGTCCGAGTGGGATGAACGCGCTtctctc < 1:2299520/69‑1 (MQ=255) ctctGCCTGGCGGCCTGTGAAGGCAAGCTGGACGAGAAAACGTCCGAGTGGGATGAACGCGCTtctctc < 1:3316613/69‑1 (MQ=255) ctctGCCTGGCGGCCTGTGAAGGCAAGCTGGACGAGAAAACGTCCGAGTGGGATGAACGCGCTtctctc < 1:1443787/69‑1 (MQ=255) ctctGCCTGGCGGCCTGTGAAGGCAAGCTGGACGAGAAAACGTCCGAGTGGGATGAACGCGCTtctctc < 1:620096/69‑1 (MQ=255) ctctGCCTGGCGGCCTGTGAAGGCAAGCTGGACGAGAAAACGTCCGAGTGGGAAGAACGCGCTtctctc < 1:1958524/69‑1 (MQ=255) ttgcggcCTGTGAAGGCAAGCTGGACGAGAAAACGTCCGAGTGGGATGAACGCGCTTCTCTCGGCgtg < 1:524317/66‑1 (MQ=255) tGGCGGCCTGTGAAGGCAAGCTTGACGAGAAAACGTCCGAGTGGGATGAACGCGCTTCTCTCGGCgtg < 1:1159029/68‑1 (MQ=255) tGGCGGCCTGTGAAGGCAAGCTGGACGAGAAAACGTCCGATTGGGATGAACGCGCTTCTCTCGGCgtg < 1:1823209/68‑1 (MQ=255) tGGCGGCCTGTGAAGGCAAGCTGGACGAGAAAACGTCCGAGTGGGATGAACGCGCTTCTCTCGGCgtg < 1:3293892/68‑1 (MQ=255) tGGCGGCCTGTGAAGGCAAGCTGGACGAGAAAACGTCCGAGTGGGATGAACGCGCTTCTCTCGGCgtg < 1:2740741/68‑1 (MQ=255) tGGCGGCCTGTGAAGGCAAGCTGGACGAGAAAACGTCCGAGTGGGATGAACGCGCTTCTCTCGGCgtg < 1:2980341/68‑1 (MQ=255) tGGCGGCCTGTGAAGGCAAGCTGGACGAGAAAACGTCCGAGTGGGATGAACGCGCTTCTCTCGGCgtg < 1:2711746/68‑1 (MQ=255) tGGCGGCCTGTGAAGGCAAGCTGGACGAGAAAACGTCCGAGTGGGATGAACGCGCTTCTCTCGGCgtg < 1:2989826/68‑1 (MQ=255) tGGCGGCCTGTGAAGGCAAGCTGGACGAGAAAACGTCCGAGTGGGATGAACGCGCTTCTCTCGGCgtg < 1:718787/68‑1 (MQ=255) tGGCGGCCTGTGAAGGCAAGCTGGACGAGAAAACGTCCGAGTGGGATGAACGCGCTTCTCTCGGCgtg < 1:705742/68‑1 (MQ=255) tGGCGGCCTGTGAAGGCAAGCTGGACGAGAAAACGTCCGAGTGGGATGAACGCGCTTCTCTCGGCgtg < 1:307309/68‑1 (MQ=255) tGGCGGCCTGTGAAGGCAAGCTGGACGAGAAAACGTCCGAGTGGGATGAACGCGCTTCTCTCGGCgtg < 1:2323808/68‑1 (MQ=255) tGGCGGCCTGTGAAGGCAAGCTGGACGAGAAAACGTCCGAGTGGGATGAACGCGCTTCTCTCGGCgtg < 1:563340/68‑1 (MQ=255) tGGCGGCCTGTGAAGGCAAGCTGGACGAGAAAACGTCCGAGTGGGATGAACGCGCTTCTCTCGGCgtg < 1:2279002/68‑1 (MQ=255) tGGCGGCCTGTGAAGGCAAGCTGGACGAGAAAACGTCCGAGTGGGATGAACGCGCTTCTCTCGGCgtg < 1:1500688/68‑1 (MQ=255) tGGCGGCCTGTGAAGGCAAGCTGGACGAGAAAACGTCCGAGTGGGATGAACGCGCTTCTCTCGGCgtg < 1:325462/68‑1 (MQ=255) tGGCGGCCTGTGAAGGCAAGCTGGACGAGAAAACGTCCGAGTGGGATGAACGCGCTTCTCTCGGCgtg < 1:1628108/68‑1 (MQ=255) tGGCGGCCTGTGAAGGCAAGCTGGACGAGAAAACGTCCGAGTGGGATGAACGCGCTTCTCTCGGCgtg < 1:2125037/68‑1 (MQ=255) tGGCGGCCTGTGAAGGCAAGCTGGACGAGAAAACGTCCGAGTGGGATGAACGCGCTTCTCTCGGCgtg < 1:2091371/68‑1 (MQ=255) tGGCGGCCTGTGAAGGCAAGCTGGACGAGAAAACGTCCGAGTGGGATGAACGCGCTTCTCTCGGCgtg < 1:1986884/68‑1 (MQ=255) ggcggcCTGTGAAAGCAAACTGGACGAGAAAACGTCCGAGTGGGATGAACGCGCTTCTCTCGGCgtggt < 1:956692/69‑1 (MQ=255) ggcggcCTGTGAAAGCAAACTGGACGAGAAAACGTCCGAGTGGGATGAACGCGCTTCTCTCGGCgtggt < 1:849635/69‑1 (MQ=255) ggcggcCTGTGAAAGCAAACTGGACGAGAAAACGTCCGAGTGGGATGAACGCGCTTCTCTCGGCgtggt < 1:3289885/69‑1 (MQ=255) ggcggcCTGTGAAAGCAAACTGGACGAGAAAACGTCCGAGTGGGATGAACGCGCTTCTCTCGGCgtggt < 1:2862189/69‑1 (MQ=255) ggcggcCTGTGAAAGCAAACTGGACGAGAAAACGTCCGAGTGGGATGAACGCGCTTCTCTCGGCgtggt < 1:1140151/69‑1 (MQ=255) ggcggcCTGTGAAAGCAAACTGGACGAGAAAACGTCCGAGTGGGATGAACGCGCTTCTCTCGGCgtggt < 1:1234886/69‑1 (MQ=255) ggcggcCTGTGAAAGCAAACTGGACGAGAAAACGTCCGAGTGGGATGAACGCGCTTCTCTCGGCgtggt < 1:1510867/69‑1 (MQ=255) ggcggcCTGTGAAAGCAAACTGGACGAGAAAACGTCCGAGTGGGATGAACGCGCTTCTCTCGGCgtggt < 1:2733066/69‑1 (MQ=255) ggcggcCTGTGAAAGCAAACTGGACGAGAAAACGTCCGAGTGGGATGAACGCGCTTCTCTCGGCgtggt < 1:1547935/69‑1 (MQ=255) ggcggcCTGTGAAAGCAAACTGGACGAGAAAACGTCCGAGTGGGATGAACGCGCTTCTCTCGGCgtggt < 1:1785760/69‑1 (MQ=255) ggcggcCTGTGAAAGCAAACTGGACGAGAAAACGTCCGAGTGGGATGAACGCGCTTCTCTCGGCgtggt < 1:1933879/69‑1 (MQ=255) ggcggcCTGTGAAAGCAAACTGGACGAGAAAACGTCCGAGTGGGATGAACGCGCTTCTCTCGGCgtggt < 1:2228764/69‑1 (MQ=255) cggcCTGTGAAGGCAAGCTGGACGAGAAAACGTCCGAGTGGGATGAACGCGCTTCTCTCGGCgtg < 1:455179/65‑1 (MQ=255) ccTGTGAAAGCAAACTGGACGAGAAAACGTCCGAGTGGGATGAACGCGCTTCTCTCGGc > 1:2836816/1‑59 (MQ=255) ccTGTGAAAGCAAACTGGACGAGAAAACGTCCGAGTGGGATGAACGCGCTTCTCTCGGCGTGGTGATgg > 1:1070313/1‑69 (MQ=255) ccTGTGAAAGCAAACTGGACGAGAAAACGTCCGAGTGGGATGAACGCGCTTCTCTCGGCGTGGTGATgg < 1:1494484/69‑1 (MQ=255) ccTGTGAAAGCAAACTGGACGAGAAAACGTCCGAGTGGGATGAACGCGCTTCTCTCGGCGTGGTGATgg > 1:2895964/1‑69 (MQ=255) ccTGTGAAAGCAAACTGGACGAGAAAACGTCCGAGTGGGATGAACGCGCTTCTCTCGGCGTGGTGATgg < 1:2718850/69‑1 (MQ=255) ccTGTGAAAGCAAACTGGACGAGAAAACGTCCGAGTGGGATGAACGCGCTTCTCTCGGCGTGGTGATgg < 1:3165671/69‑1 (MQ=255) ccTGTGAAAGCAAACTGGACGAGAAAACGTCCGAGTGGGATGAACGCGCTTCTCTCGGCGTGGTGATgg < 1:1595981/69‑1 (MQ=255) ccTGTGAAAGCAAACTGGACGAGAAAACGTCCGAGTGGGATGAACGCGCTTCTCTCGGCGTGGTGATgg < 1:1486269/69‑1 (MQ=255) ccTGTGAAAGCAAACTGGACGAGAAAACGTCCGAGTGGGATGAACGCGCTTCTCTCGGCGTGGTGATgg < 1:1472388/69‑1 (MQ=255) ccTGTGAAAGCAAACTGGACGAGAAAACGTCCGAGTGGGATGAACGCGCTTCTCTCGGCGTGGTGATgg > 1:1380999/1‑69 (MQ=255) ccTGTGAAAGCAAACTGGACGAGAAAACGTCCGAGTGGGATGAACGCGCTTCTCTCGGCGTGGTGATgg > 1:1264610/1‑69 (MQ=255) ccTGTGAAAGCAAACTGGACGAGAAAACGTCCGAGTGGGATGAACGCGCTTCTCTCGGCGTGGTGATgg < 1:439337/69‑1 (MQ=255) ccTGTGAAAGCAAACTGGACGAGAAAACGTCCGAGTGGGATGAACGCGCTTCTCTCGGCGTGGTGATgg > 1:1709605/1‑69 (MQ=255) ccTGTGAAAGCAAACTGGACGAGAAAACGTCCGAGTGGGATGAACGCGCTTCTCTCGGCGTGGTGATgg < 1:762670/69‑1 (MQ=255) ccTGTGAAAGCAAACTGGACGAGAAAACGTCCGAGTGGGATGAACGCGCTTCTCTCGGCGTGGTGATgg > 1:1915717/1‑69 (MQ=255) ccTGTGAAAGCAAACTGGACGAGAAAACGTCCGAGTGGGATGAACGCGCTTCTCTCGGCGTGGTGATgg > 1:3028899/1‑69 (MQ=255) ccTGTGAAAGCAAACTGGACGAGAAAACGTCCGAGTGGGATGAACGCGCTTCTCTCGGCGTGGTGATgg < 1:2179584/69‑1 (MQ=255) ccTGTGAAAGCAAACTGGACGAGAAAACGTCCGAGTGGGATGAACGCGCTTCTCTCGGCGTGGTGATgg < 1:2491556/69‑1 (MQ=255) ccTGTGAAAGCAAACTGGACGAGAAAACGTCCGAGTGGGATGAACGCGCTTCTCTCGGCGTGGTGATgg > 1:2754343/1‑69 (MQ=255) ccTGTGAAAGCAAACTGGACGAGAAAACGTCCGAGTGGGATGAACGCGCTTCTCTCGGCGTGCTGATgg < 1:443223/69‑1 (MQ=255) aaaGCAAACTGGACGAGAAAACGTCCGAGTGGGATGAAGGCGCTTCTCTCGGCGTGGTGATGGCTGCgg > 1:2368558/1‑69 (MQ=255) aaaGCAAACTGGACGAGAAAACGTCCGAGTGGGATGAACGCGCTTCTCTCGGCGTGGTGATGGCTGCgg > 1:1601417/1‑69 (MQ=255) aaaGCAAACTGGACGAGAAAACGTCCGAGTGGGATGAACGCGCTTCTCTCGGCGTGGTGATGGCTGCgg > 1:1651265/1‑69 (MQ=255) aaaGCAAACTGGACGAGAAAACGTCCGAGTGGGATGAACGCGCTTCTCTCGGCGTGGTGATGGCTGCgg > 1:485021/1‑69 (MQ=255) aaaGCAAACTGGACGAGAAAACGTCCGAGTGGGATGAACGCGCTTCTCTCGGCGTGGTGATGGCTGCgg > 1:2699861/1‑69 (MQ=255) aaaGCAAACTGGACGAGAAAACGTCCGAGTGGGATGAACGCGCTTCTCTCGGCGTGGTGATGGCTGCgg > 1:2749609/1‑69 (MQ=255) aaaGCAAACTGGACGAGAAAACGTCCGAGTGGGATGAACGCGCTTCTCTCGGCGTGGTGATGGCTGCgg > 1:3008525/1‑69 (MQ=255) aaaGCAAACTGGACGAGAAAACGTCCGAGTGGGATGAACGCGCTTCTCTCGGCGTGGTGATGGCTGCgg > 1:2251838/1‑69 (MQ=255) aaaGCAAACTGGACGAGAAAACGTCCGAGTGGGATGAACGCGCTTCTCTCGGCGTGGTGATGGCTGCgg > 1:889520/1‑69 (MQ=255) aaaGCAAACTGGACGAGAAAACGTCCGAGTGGGATGAACGCGCTTCTCTCGGCGTGGTGATGGCTGCg < 1:1332570/68‑1 (MQ=255) cAAGCTGGACGAGAAAACGTCCGAGTGGGATGAACGCGCTTCTCTCGGCgtg < 1:1571970/52‑1 (MQ=255) aaaCTGGACGAGAAAACGTCCGAGTGGGATGAACGCGCTTCTCTCGGCGTGGTGATGGCTGCGGGTGGa < 1:1138875/69‑1 (MQ=255) aaaCTGGACGAGAAAACGTCCGAGTGGGATGAACGCGCTTCTCTCGGCGTGGTGATGGCTGCGGGTGGa < 1:1033484/69‑1 (MQ=255) | AACCCAGCCGATTATGCTGCGCATGAAGTCCGATCTGGTTGAGCTCTGCCTGGCGGCCTGTGAAAGCAAACTGGACGAGAAAACGTCCGAGTGGGATGAACGCGCTTCTCTCGGCGTGGTGATGGCTGCGGGTGGA > minE/2237340‑2237475 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |