| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,239,637 | A→T | 53.3% | P275P (CCT→CCA) | zraS ← | sensory histidine kinase in two‑component regulatory system with ZraR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,239,637 | 0 | A | T | 53.3% | ‑1.0 / 50.2 | 45 | P275P (CCT→CCA) | zraS | sensory histidine kinase in two‑component regulatory system with ZraR |
| Reads supporting (aligned to +/- strand): ref base A (7/14); new base T (5/19); total (12/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.01e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.05e-01 | |||||||||||
TAAACGGTCGGCCTCTTTCGCCATCACCTGCGCCAGTTGATGCGCTTCTCCCCCTGCAGGCGCGCGCTCGGCAAAGTATTTCGCCAGTCCTTTAATCGAGGAAAGTG > minE/2239580‑2239686 | tAAACGGTCGGCCTCTTTCGCCATCACCTGCGCCAGTTGATGCGCTTCTCCCCCTGCAGGCGCGCGCTc > 1:1110570/1‑69 (MQ=255)tAAACGGTCGGCCTCTTTCGCCATCACCTGCGCCAGTTGATGCGCTTCTCCCCCTGCAGGCGCGCGCTc > 1:737107/1‑69 (MQ=255) aCGGTCAGCTTCTTTCGCCATCACCTGCGCCAGTTGATGCGCTTCTCCCCCTGCTGgcgcgcg < 1:2555350/63‑1 (MQ=255) aCGGTCAGCTTCTTTCGCCATCACCTGCGCCAGTTGATGCGCTTCTCCCCCTGCTGgcgcgcg < 1:940454/63‑1 (MQ=255) aCGGTCAGCTTCTTTCGCCATCACCTGCGCCAGTTGATGCGCTTCTCCCCCTGCTGgcgcgcg < 1:910622/63‑1 (MQ=255) aCGGTCAGCTTCTTTCGCCATCACCTGCGCCAGTTGATGCGCTTCTCCCCCTGCTGgcgcgcg < 1:75288/63‑1 (MQ=255) aCGGTCAGCTTCTTTCGCCATCACCTGCGCCAGTTGATGCGCTTCTCCCCCTGCTGgcgcgcg < 1:696294/63‑1 (MQ=255) aCGGTCAGCTTCTTTCGCCATCACCTGCGCCAGTTGATGCGCTTCTCCCCCTGCTGgcgcgcg < 1:681090/63‑1 (MQ=255) aCGGTCAGCTTCTTTCGCCATCACCTGCGCCAGTTGATGCGCTTCTCCCCCTGCTGgcgcgcg < 1:565001/63‑1 (MQ=255) aCGGTCAGCTTCTTTCGCCATCACCTGCGCCAGTTGATGCGCTTCTCCCCCTGCTGgcgcgcg < 1:3223709/63‑1 (MQ=255) aCGGTCAGCTTCTTTCGCCATCACCTGCGCCAGTTGATGCGCTTCTCCCCCTGCTGgcgcgcg < 1:3065045/63‑1 (MQ=255) aCGGTCAGCTTCTTTCGCCATCACCTGCGCCAGTTGATGCGCTTCTCCCCCTGCTGgcgcgcg < 1:2710850/63‑1 (MQ=255) aCGGTCAGCTTCTTTCGCCATCACCTGCGCCAGTTGATGCGCTTCTCCCCCTGCTGgcgcgcg < 1:2644933/63‑1 (MQ=255) aCGGTCAGCTTCTTTCGCCATCACCTGCGCCAGTTGATGCGCTTCTCCCCCTGCTGgcgcgcg < 1:1007988/63‑1 (MQ=255) aCGGTCAGCTTCTTTCGCCATCACCTGCGCCAGTTGATGCGCTTCTCCCCCTGCTGgcgcgcg < 1:1186291/63‑1 (MQ=255) aCGGTCAGCTTCTTTCGCCATCACCTGCGCCAGTTGATGCGCTTCTCCCCCTGCTGgcgcgcg < 1:2045365/63‑1 (MQ=255) aCGGTCAGCTTCTTTCGCCATCACCTGCGCCAGTTGATGCGCTACTCCCCCTGCTGgcgcgcg < 1:2264090/63‑1 (MQ=255) cGGTCAGCTTCTTTCGCCATCACCTGCGCCAGTTGATGCGCTTCTCCCCCTGCTGgcgcgcg < 1:2362985/62‑1 (MQ=255) cGGTCAGCTTCTTTCGCCATCACCTGCGCCAGTTGATGCGCTTCTCCCCCTGCTGgcgcgcg < 1:2668752/62‑1 (MQ=255) gTCGGCCTCTTTCGCCATCACCTGCGCCAGTTGATGCGCTTCTCCCCCTGCAGGCGCGCGCTCGGCaa > 1:1036475/1‑68 (MQ=255) tCGCCATCACCTGCGCCAGTTGATGCGCTTCTCCCCCTGCTGgcgcgcg < 1:377146/49‑1 (MQ=255) aCCTGCGCCAGTTGATGCGCTTCTCCCCCTGCAGGCGCGCGCTCGGCAAAGTATTTCGCCAGTCCTTTa > 1:3382857/1‑69 (MQ=255) aCCTGCGCCAGTTGATGCGCTTCTCCCCCTGCAGGCGCGCGCTCGGCAAAGTATTTAGCCAGTCCTTTa > 1:732969/1‑69 (MQ=255) aGTTGATGCGCTTCTCCCCCTGCAGGCGCGCGCTCGGCAAAGTATTTCGCCAGTCCTTTAATCGAGGaa < 1:170326/69‑1 (MQ=255) aGTTGATGCGCTTCTCCCCCTGCAGGCGCGCGCTCGGCAAAGTATTTCGCCAGTCCTTTAATCGAGGaa < 1:1081666/69‑1 (MQ=255) aGTTGATGCGCTTCTCCCCCTGCAGGCGCGCGCTCGGCAAAGTATTTCGCCAGTCCTTTAATCGAGGaa < 1:1157260/69‑1 (MQ=255) aGTTGATGCGCTTCTCCCCCTGCAGGCGCGCGCTCGGCAAAGTATTTCGCCAGTCCTTTAATCGAGGaa < 1:687112/69‑1 (MQ=255) aGTTGATGCGCTTCTCCCCCTGCAGGCGCGCGCTCGGCAAAGTATTTCGCCAGTCCTTTAATCGAGGaa < 1:553466/69‑1 (MQ=255) aGTTGATGCGCTTCTCCCCCTGCAGGCGCGCGCTCGGCAAAGTATTTCGCCAGTCCTTTAATCGAGGaa < 1:2183390/69‑1 (MQ=255) aGTTGATGCGCTTCTCCCCCTGCAGGCGCGCGCTCGGCAAAGTATTTCGCCAGTCCTTTAATCGAGGaa < 1:3000903/69‑1 (MQ=255) aGTTGATGCGCTTCTCCCCCTGCAGGCGCGCGCTCGGCAAAGTATTTCGCCAGTCCTTTAATAGAGGaa < 1:325727/69‑1 (MQ=255) gTTGATGCGCTTCTCCCCCTGCAGGCGCGCGCTCGGCAAAGTATTTCGCCAGTCCTTTAATCGAGGaa < 1:3088118/68‑1 (MQ=255) gTTGATGCGCTTCTCCCCCTGCAGGCGCGCGCTCGGCAAAGTATTTCGCCAGTCCTTTAATCGAGGaa < 1:2501904/68‑1 (MQ=255) gATGCGCTTCTCCCCCTGCTGGCGCGCGCTCGGCAAAGTATTTCGCCAGTCCTTTAATCGAGGAAAGTg < 1:2175706/69‑1 (MQ=255) gATGCGCTTCTCCCCCTGCAGGCGCGCGCTCGGCAAAGTATTTCGCCAGTCCTTTAATCGAGGAAAGt > 1:2338035/1‑68 (MQ=255) gATGCGCTTCTCCCCCTGCAGGCGCGCGCTCGGCAAAGTATTTCGCCAGTCCTTTAATCGAGGAAAGTg > 1:1257739/1‑69 (MQ=255) gATGCGCTTCTCCCCCTGCAGGCGCGCGCTCGGCAAAGTATTTCGCCAGTCCTTTAATCGAGGAAAGTg < 1:2618169/69‑1 (MQ=255) gATGCGCTTCTCCCCCTGCAGGCGCGCGCTCGGCAAAGTATTTCGCCAGTCCTTTAATCGAGGAAAGTg < 1:1789626/69‑1 (MQ=255) gATGCGCTTCTCCCCCTGCAGGCGCGCGCTCGGCAAAGTATTTCGCCAGTCCTTTAATCGAGGAAAGTg < 1:2127210/69‑1 (MQ=255) tGCGCTTCTCCCCCTGCTGGCGCGCGTTCGGCAAAGTAt > 1:1204145/1‑39 (MQ=38) tGCGCTTCTCCCCCTGCTGGCGCGCGTTCGGCAAAGTAt > 1:1829508/1‑39 (MQ=38) tGCGCTTCTCCCCCTGCTGGCGCGCGTTCGGCAAAGTAt > 1:2549596/1‑39 (MQ=38) tGCGCTTCTCCCCCTGCTGGCGCGCGTTCGGCAAAGTAt > 1:1171044/1‑39 (MQ=38) agcgcTTCTCCCCCTGCTGGCGCGCGTTCGGCAAAGTAt > 1:1274620/2‑39 (MQ=37) cTGCAGGCGCGCGCTCGGCAAAGTATTTCGCCAGTCCTTTAATCGAGGaa < 1:2936556/50‑1 (MQ=255) | TAAACGGTCGGCCTCTTTCGCCATCACCTGCGCCAGTTGATGCGCTTCTCCCCCTGCAGGCGCGCGCTCGGCAAAGTATTTCGCCAGTCCTTTAATCGAGGAAAGTG > minE/2239580‑2239686 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |