| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,243,898 | G→C | 46.1% | A258A (GCC→GCG) | hemE ← | uroporphyrinogen decarboxylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,243,898 | 0 | G | C | 46.1% | 8.1 / 56.5 | 52 | A258A (GCC→GCG) | hemE | uroporphyrinogen decarboxylase |
| Reads supporting (aligned to +/- strand): ref base G (21/7); new base C (20/4); total (41/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.16e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGGCGGCGCGCATCGGCGATGTCCGTTGTCCAGTCGAGGCCCAGCGCATCGCAACCGGTTTCTGCCATGGCTTCCAGCCACTGTCCGCCGCCTTTGGTAAACAGCGTGACCGGTACGCGGCGACCGTC > minE/2243830‑2243957 | cggcggCGCGCATCGGCGATGTCCGTTGTCCAGTCGAGGCCCAGCGCATCGCAACCGGTTTCTGCCATg < 1:1274920/69‑1 (MQ=255)cggcggCGCGCATCGGCGATGTCCGTTGTCCAGTCGAGGCCCAGCGCATCGCAACCGGTTTCTGCCATg < 1:541888/69‑1 (MQ=255)cggcggCGCGCATCGGCGATGTCCGTTGTCCAGTCGAGGCCCAGCGCATCGCAACCGGTTTCTGCCATg < 1:896928/69‑1 (MQ=255)cggcggCGCGCATCGGCGATGTCCGTTGTCCAGTCGAGGCCCAGCGCATCGCAACCGGTTTCTGCCATg < 1:618229/69‑1 (MQ=255) gcgcATCGGCGATGTCCGTTGTCCAGTCGAGGCCCAGCGCATCGCAACCGGTTTCTGCCATGGCTTCCa > 1:2802401/1‑69 (MQ=255) gcgcATCGGCGATGTCCGTTGTCCAGTCGAGGCCCAGCGCATCGCAACCGGTTTCTGCCATGGCTTCCa > 1:3100350/1‑69 (MQ=255) gcgcATCGGCGATGTCCGTTGTCCAGTCGAGGCCCAGCGCATCGCAACCGGTTTCTGCCATGGCTTCCa > 1:2054095/1‑69 (MQ=255) gcgcATCGGCGATGTCCGTTGTCCAGTCGAGGCCCAGCGCATCGCAACCGGTTTCTGCCATGGCTTCCa > 1:1911690/1‑69 (MQ=255) gcgcATCGGCGATGTCCGTTGTCCAGTCGAGGCCCAGCGCATCGCAACCGGTTTCTGCCATGGCTTCCa > 1:563596/1‑69 (MQ=255) gcgcATCGGCGATGTCCGTTGTCCAGTCGAGGCCCAGCGCATCGCAACCGGTTTCTGCCATGGCTTCCa > 1:938848/1‑69 (MQ=255) gcgcATCGGCGATGTCCGTTGTCCAGTCGAGGCCCAGCGCATCGCAACCGGTTTCTGCCATGGCTTCCa > 1:1252535/1‑69 (MQ=255) tCCGTTGTCCAGTCGAGGCCCAGCGCATCGCAACCGGTTTCTGCCATGGCTTCCAGCCACTGTccgccg > 1:1306961/1‑69 (MQ=255) gTCCAGTCGAGGCCCAGCGCATCGCAACCGGTTTCTGCCATGGCTTCCAGCCACTGTCCGCCGCCttt > 1:3007127/1‑68 (MQ=255) gTCGAGGCCCAGCGCATCGCAACCGGTTTCTGCCATGGCTTCCAGCCACTGTCCGCCGCCTTTGGTa > 1:886902/1‑67 (MQ=255) aGCGCATCGCAACCGGTTTCTGCCATCGCTTCCAGCCACTGTCCACCGCCTTTAGTAAACAGCGTGAct > 1:3327745/1‑68 (MQ=255) aGCGCATCGCAACCGGTTTCTGCCATCGCTTCCAGCCACTGTCCACCGCCTTTAGTAAACAGCGTGAct > 1:2809101/1‑68 (MQ=255) aGCGCATCGCAACCGGTTTCTGCCATCGCTTCCAGCCACTGTCCACCGCCTTTAGTAAACAGCGTGAct > 1:2805154/1‑68 (MQ=255) aGCGCATCGCAACCGGTTTCTGCCATCGCTTCCAGCCACTGTCCACCGCCTTTAGTAAACAGCGTGAct > 1:100809/1‑68 (MQ=255) aGCGCATCGCAACCGGTTTCTGCCATCGCTTCCAGCCACTGTCCACCGCCTTTAGTAAACAGCGTGAct > 1:2838181/1‑68 (MQ=255) aGCGCATCGCAACCGGTTTCTGCCATCGCTTCCAGCCACTGTCCACCGCCTTTAGTAAACAGCGTGAct > 1:2906510/1‑68 (MQ=255) aGCGCATCGCAACCGGTTTCTGCCATCGCTTCCAGCCACTGTCCACCGCCTTTAGTAAACAGCGTGAct > 1:988620/1‑68 (MQ=255) aGCGCATCGCAACCGGTTTCTGCCATCGCTTCCAGCCACTGTCCACCGCCTTTAGTAAACAGCGTGAct > 1:3271092/1‑68 (MQ=255) aGCGCATCGCAACCGGTTTCTGCCATCGCTTCCAGCCACTGTCCACCGCCTTTAGTAAACAGCGTGAct > 1:787252/1‑68 (MQ=255) aGCGCATCGCAACCGGTTTCTGCCATCGCTTCCAGCCACTGTCCACCGCCTTTAGTAAACAGCGTGAct > 1:353163/1‑68 (MQ=255) aGCGCATCGCAACCGGTTTCTGCCATCGCTTCCAGCCACTGTCCACCGCCTTTAGTAAACAGCGTGAct > 1:429377/1‑68 (MQ=255) aGCGCATCGCAACCGGTTTCTGCCATCGCTTCCAGCCACTGTCCACCGCCTTTAGTAAACAGCGTGAct > 1:937933/1‑68 (MQ=255) aGCGCATCGCAACCGGTTTCTGCCATCGCTTCCAGCCACTGTCCACCGCCTTTAGTAAACAGCGTGAct > 1:649829/1‑68 (MQ=255) aGCGCATCGCAACCGGTTTCTGCCATCGCTTCCAGCCACTGTCCACCGCCTTTAGTAAACAGCGTGAct > 1:2802292/1‑68 (MQ=255) aGCGCATCGCAACCGGTTTCTGCCATCGCTTCCAGCCACTGTCCACCGCCTTTAGTAAACAGCGTGAct > 1:1765819/1‑68 (MQ=255) aGCGCATCGCAACCGGTTTCTGCCATCGCTTCCAGCCACTGTCCACCGCCTTTAGTAAACAGCGTGAct > 1:2608584/1‑68 (MQ=255) aGCGCATCGCAACCGGTTTCTGCCATCGCTTCCAGCCACTGTCCACCGCCTTTAGTAAACAGCGTGAct > 1:1500235/1‑68 (MQ=255) aGCGCATCGCAACCGGTTTCTGCCATCGCTTCCAGCCACTGTCCACCGCCTTTAGTAAACAGCGTGAct > 1:2488820/1‑68 (MQ=255) aGCGCATCGCAACCGGTTTCTGCCATCGCTTCCAGCCACTGTCCACCGCCTTAAGTAAAc > 1:1499339/1‑60 (MQ=255) aGCGCATCGCAACCGGTTTCTGCCATCGCTTCCAGCCACTATCCACCGCCTTTAGTAAACAGCCTGAct > 1:901439/1‑68 (MQ=255) cgcATCGCAACCGGTTTCTGCCATGGCTTCCAGCCACTGTCCGCCGCCTTTGGTAAACAGCGTGACCgg < 1:563501/69‑1 (MQ=255) cgcATCGCAACCGGTTTCTGCCATGGCTTCCAGCCACTGTCCGCCGCCTTTGGTAAACAGCGTGACCgg < 1:2108998/69‑1 (MQ=255) cgcATCGCAACCGGTTTCTGCCATGGCTTCCAGCCACTGTCCGCCGCCTTTGGTAAACAGCGTGACCgg < 1:3248299/69‑1 (MQ=255) cgcATCGCAACCGGTTTCTGCCATGGCTTCCAGCCACTGTCCGCCGCCTTTGGTAAACAGCGTGACCgg < 1:3018755/69‑1 (MQ=255) cgcATCGCAACCGGTTTCTGCCATGGCTTCCAGCCACTGTCCGCCGCCTTTGGTAAACAGCGTGACCgg < 1:965490/69‑1 (MQ=255) cGCAACCGGTTTCTGCCATGGCTTCCAGCCACTGTCCGCCGCCTTTGGTAAACAGCGTGACCGGTAcgc > 1:2576907/1‑69 (MQ=255) cGCAACCGGTTTCTGCCATGGCTTCCAGCCACTGTCCGCCGCCTTTGGTAAACAGCGTGACCGGTAcgc > 1:1858506/1‑69 (MQ=255) cGCAACCGGTTTCTGCCATGGCTTCCAGCCACTGTCCGCCGCCTTTGGTAAACAGCGTGACCGGTAcgc > 1:575299/1‑69 (MQ=255) cGCAACCGGTTTCTGCCATGGCTTCCAGCCACTGTCCGCCGCCTTTGGTAAACAGCGTGACCGGTAcg > 1:2444350/1‑68 (MQ=255) cGCAACAGGTTTCTGCCATGGCTTCCAGCCACTGTCCGCCGCCTTTGGTAAACAGCGTGACCGGTAcgc > 1:2723/1‑69 (MQ=255) ggTTTCTGCCATGGCTTCCAGCCACTGTCCGCCGCCTTTGGTAAACAGCGt > 1:2068401/1‑51 (MQ=255) ggTTTCTGCCATGGCTTCCAGCCACTGTCCGCCGCCTTTGGTAAACAGCGt > 1:144936/1‑51 (MQ=255) ggTTTCTGCCATCGCTTCCAGCCACTGTCCACCGCCTTTAGTAAACAGCGTGACTGGTACGCGGCGAcc < 1:2249812/69‑1 (MQ=255) ggTTTCTGCCATCGCTTCCAGCCACTGTCCACCGCCTTTAGTAAACAGCGTGACTGGTACGCGGCGAcc < 1:1497121/69‑1 (MQ=255) ggTTTCTGCCATCGCTTCCAGCCACTGTCCACCGCCTTTAGTAAACAGCGTGACTGGTACGCGGCGAcc < 1:2828527/69‑1 (MQ=255) ggTTTCTGCCATCGCTTCCAGCCACTGTCCACCGCCTTTAGTAAACAGCGTGACTGGTACGCGGCGAcc < 1:1221147/69‑1 (MQ=255) gTTTCTGCCATGGCTTCCAGCCACTGTCCGCCGCCTTTGGTAAACAGCGTGACCGGTACgcggcg > 1:1851053/1‑65 (MQ=255) gTTTCTGCCATGGCTTCCAGCCACTGTCCGCCGCCTTTGGTAAACAGCGTGACCGGTACgcggcg > 1:542826/1‑65 (MQ=255) gTTTCTGCCATGGCTTCCAGCCACTGTCCGCCGCCTTTGGTAAACAGCGTGACCGGTACgcggcg > 1:495401/1‑65 (MQ=255) gTTTCTGCCATGGCTTCCAGCCACTGTCCGCCGCCTTTGGTAAACAGCGTGACCGGTACgcggcg > 1:1602105/1‑65 (MQ=255) ttCTGCCATGGCTTCCAGCCACTGTCCGCCGCCTTTGGTAAACAGCGTGACCGGTACGCGGCGACcgtc < 1:1789735/69‑1 (MQ=255) ccATGGCTTCCAGCCACTGTCCGCCGCCTTTGGTAAACAGCGTGACCgg < 1:2438057/49‑1 (MQ=255) | CGGCGGCGCGCATCGGCGATGTCCGTTGTCCAGTCGAGGCCCAGCGCATCGCAACCGGTTTCTGCCATGGCTTCCAGCCACTGTCCGCCGCCTTTGGTAAACAGCGTGACCGGTACGCGGCGACCGTC > minE/2243830‑2243957 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |