| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,245,543 | T→A | 15.3% | intergenic (‑59/‑36) | nudC ← / → rsd | NADH pyrophosphatase/stationary phase protein, binds sigma 70 RNA polymerase subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,245,543 | 0 | T | A | 15.3% | 215.3 / 27.6 | 104 | intergenic (‑59/‑36) | nudC/rsd | NADH pyrophosphatase/stationary phase protein, binds sigma 70 RNA polymerase subunit |
| Reads supporting (aligned to +/- strand): ref base T (35/53); new base A (3/13); total (38/66) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.59e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.43e-01 | |||||||||||
Only 100 of 104 total aligned reads displayed.
GCTCTTGCACTACCTTTGCATCACTGGCATGTTTAACATGGTTTTTACATTTCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCAATCATGCTTAACCAGCTCGATAACCTGACGG > minE/2245486‑2245606 | gCTCTTGCACTACCTTTGCATCACTGGCATGTTTAACATGGTTTTTACATTTCTCACTTAGCAGtttt > 1:910216/1‑68 (MQ=255)gCTCTTGCACTACCTTTGCATCACTGGCATGTTTAACATGGTTTTTACATTTCTCACTGAGCAGtttt > 1:1128930/1‑68 (MQ=255)gCTCTTGCACTACCTTTGCATCACTGGCATGTTTAACATGGTTTTTACATTTCTCACTGAGCAGtttt > 1:1440277/1‑68 (MQ=255)gCTCTTGCACTACCTTTGCATCACTGGCATGTTTAACATGGTTTTTACATTTCTCACTGAGCAGtttt > 1:19528/1‑68 (MQ=255)gCTCTTGCACTACCTTTGCATCACTGGCATGTTTAACATGGTTTTTACATTTCTCACTGAGCAGtttt > 1:820616/1‑68 (MQ=255)gCTCTTGCACTACCTTTGCATCACTGGCATGTTTAACATGGTTTTTACATTTCTCACTGAGCAGtttt > 1:642375/1‑68 (MQ=255)gCTCTTGCACTACCTTTGCATCACTGGCATGTTTAACATGGTTTTTACATTTCTCACTGAGCAGtttt > 1:1109361/1‑68 (MQ=255)gCTCTTGCACTACCTTTGCATCACTGGCATGTTTAACATGGTTTTTACATTTCTCACTGAGCAGtttt > 1:1108156/1‑68 (MQ=255)gCTCTTGCACTACCTTTGCATCACTGGCATGTTTAACATGGTTTTTACATTTCTCACTGAGCAGtttt > 1:1080087/1‑68 (MQ=255) tctTGCACTACCTTTGCATCACTGGCATGTTTAACATGGTTTTTACATTTCTCACTGAGCAGTTTTTg < 1:1092909/68‑1 (MQ=255) tctTGCACTACCTTTGCATCACTGGCATGTTTAACATGGTTTTTACATTTCTCACTGAGCAGTTTTTg < 1:1857942/68‑1 (MQ=255) tctTGCACTACCTTTGCATCACTGGCATGTTTAACATGGTTTTTACATTTCTCACTGAGCAGTTTTTg < 1:2827938/68‑1 (MQ=255) tctTGCACTACCTTTGCATCACTGGCATGTTTAACATGGTTTTTACATTTCTCACTGAGCAGTTTTTg < 1:3087882/68‑1 (MQ=255) tctTGCACTACCTTTGCATCACTGGCATGTTTAACATGGTTTTTACATTTCTCACTGAGCAGTTTTTg < 1:3127431/68‑1 (MQ=255) tctTGCACTACCTTTGCATCACTGGCATGTTTAACATGGTTTTTACATTTCTCACTGAGCAGTTTTTg < 1:1583139/68‑1 (MQ=255) gCACTACCTTTGCATCACTGGCATGTTTAACATGGTTTTTACATTTCTCACTGAGCAGtttt > 1:2065381/1‑62 (MQ=255) gCACTACCTTTGCATCACTGGCATGTTTAACATGGTTTTTACATTTCTCACTGAGCAGTTTTTGAATa > 1:2725833/1‑68 (MQ=255) gCACTACCTTTGCATCACTGGCATGTTTAACATGGTTTTTACATTTCTCACTGAGCAGTTTTTGAATa > 1:77835/1‑68 (MQ=255) gCACTACCTTTGCATCACTGGCATGTTTAACATGGTTTTTACATTTCTCACTGAGCAGTTTTTGAATa > 1:2043370/1‑68 (MQ=255) cTACCTTTGCATCACTGGCATGTTTAACATGGTTTTTACATTTCTCACTGAGCAGTTTTTGAATACaa < 1:1325424/68‑1 (MQ=255) cTACCTTTGCATCACTGGCATGTTTAACATGGTTTTTACATTTCTCACTGAGCAGTTTTTGAATACaa < 1:982603/68‑1 (MQ=255) cTACCTTTGCATCACTGGCATGTTTAACATGGTTTTTACATTTCTCACTGAGCAGTTTTTGAATACaa < 1:414266/68‑1 (MQ=255) cTACCTTTGCATCACTGGCATGTTTAACATGGTTTTTACATTTCTCACTGAGCAGTTTTTGAATACaa < 1:44812/68‑1 (MQ=255) cTACCTTTGCATCACTGGCATGTTTAACATGGTTTTTACATTTCTCACTGAGCAGTTTTTGAATACaa < 1:100790/68‑1 (MQ=255) aCCTTTGCATCACTGGCATGTTTAACATGGTTTTTACATTTCTCACTGAGCAGTTTTTGAATACAAACt < 1:1542423/69‑1 (MQ=255) aCCTTTGCATCACTGGCATGTTTAACATGGTTTTTACATTTCTCACTGAGCAGTTTTTGAATACAAACt < 1:1542327/69‑1 (MQ=255) aCCTTTGCATCACTGGCATGTTTAACATGGTTTTTACATTTCTCACTGAGCAGTTTTTGAATACAAACt < 1:717619/69‑1 (MQ=255) cATCACTGGCATGTTTAACATGGTTTTTACATTTCTCACTGAGCAGTTTTTGAATACAAACTTGCGGa > 1:518660/1‑68 (MQ=255) cATCACTGGCATGTTTAACATGGTTTTTACATTTCTCACAGAGCAGTTTTTGAATACAAACTTGCGGa < 1:206747/68‑1 (MQ=255) cATCACTGGCATGTTTAACATGGTTTTTACATTTCTCACAGAGCAGTTTTTGAATACAAACTTGCGGa < 1:3111142/68‑1 (MQ=255) cATCACTGGCATGTTTAACATGGTTTTTACATTTCTCACAGAGCAGTTTTTGAATACAAACTTGCGGa < 1:772938/68‑1 (MQ=255) cATCACTGGCATGTTTAACATGGTTTTTACATTTCTCACAGAGCAGTTTTTGAATACAAACTTGCGGa < 1:2051754/68‑1 (MQ=255) cATCACTGGCATGTTTAACATGGTTTTTACATTTCTCACAGAGCAGTTTTTGAATACAAACTTGCGGa < 1:1291451/68‑1 (MQ=255) cATCACTGGCATGTTTAACATGGTTTTTACATTTCTCACAGAGCAGTTTTTGAATACAAACTTGCGGa < 1:128544/68‑1 (MQ=255) cATCACTGGCATGTTTAACATGGTTTTTACATTTCTCACAGAGCAGTTTTTGAATACAAACTTGCGGa < 1:2588786/68‑1 (MQ=255) cATCACTGGCATGTTTAACATGGTTTTTACATTTCTCACAGAGCAGTTTTTGAATACAAACTTGCGGa < 1:2676885/68‑1 (MQ=255) cATCACTGGCATGTTTAACATGGTTTTTACATTTCTCACAGAGCAGTTTTTGAATACAAACTTGCGGa < 1:890068/68‑1 (MQ=255) cATCACTGGCATGTTTAACATGGTTTTTACATTTCTCACAGAGCAGTTTTTGAATACAAACTTGCGGa < 1:2025842/68‑1 (MQ=255) cATCACTGGCATGTTTAACATGGTTTTTACATTTCTAACAGAGCAGTTTTTGAATACAAACTTGCGGa < 1:944529/68‑1 (MQ=255) aTCACTGGCATGTTTAACATGGTTTTTACATTTCTCACAGAGCAGTTTTTGAATACAAACTTGCGGa < 1:496832/67‑1 (MQ=255) cTGGCATGTTTAACATGGTTTTTACATTTCTCACAGAGCAGTTTTTTAATACAAACTTGCGGa < 1:61593/63‑1 (MQ=255) tGGCATGTTTAACATGGTTTTTACATTTCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCAAt < 1:2601830/68‑1 (MQ=255) tGGCATGTTTAACATGGTTTTTACATTTCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCAAt < 1:269562/68‑1 (MQ=255) tGGCATGTTTAACATGGTTTTTACATTTCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCAAt < 1:804200/68‑1 (MQ=255) tGGCATGTTTAACATGGTTTTTACATTTCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCAAt < 1:1022667/68‑1 (MQ=255) tGGCATGTTTAACATGGTTTTTACATTTCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCAAt < 1:2282546/68‑1 (MQ=255) tGGCATGTTTAACATGGTTTTTACATTTCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCAAt < 1:1897111/68‑1 (MQ=255) tGGCATGTTTAACATGGTTTTTACATTTCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCAAt < 1:1968239/68‑1 (MQ=255) tGGCATGTTTAACATGGTTTTTACATTTCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCAAt < 1:1832928/68‑1 (MQ=255) tGGCATGTTTAACATGGTTTTTACATTTCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCAAt < 1:1998254/68‑1 (MQ=255) tGGCATGTTTAACATGGTTTTTACATTTCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCAAt < 1:1837027/68‑1 (MQ=255) tGGCATGTTTAACATGGTTTTTACATTTCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCAAt < 1:1679693/68‑1 (MQ=255) gCATGTTTAACATGGTTTTTACATTTCTCACAGAGCAGTTTTTGAATACAAACTTGCGGAGTCAATCAt > 1:1502926/1‑69 (MQ=255) gCATGTTTAACATGGTTTTTACATTTCTCACAGAGCAGTTTTTGAATACAAACTTGCGGAGTCAATCAt > 1:825972/1‑69 (MQ=255) gCATGTTTAACATGGTTTTTACATTTCTCACAGAGCAGTTTTTGAATACAAACTTGCGGAGTCAATCAt > 1:2954008/1‑69 (MQ=255) tttAACATGGTTTTTACATTTCTCACTGAGCAGTTTTTGAATACa > 1:2489201/1‑45 (MQ=255) tttAACATGGTTTTTACATTTCTCACTGAGCAGTTTTTGAATACa > 1:1738804/1‑45 (MQ=255) tttAACATGGTTTTTACATTTCTCACTGAGCAGTTTTTGAATACa > 1:2352486/1‑45 (MQ=255) tttAACATGGTTTTTACATTTCTCACTGAGCAGTTTTTGAATACa > 1:2705388/1‑45 (MQ=255) tttAACATGGTTTTTACATTTCTCACTGAGCAGTTTTTGAATACa > 1:382509/1‑45 (MQ=255) tttAACATGGTTTTTACATTTCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCAATCAt > 1:1451141/1‑64 (MQ=255) tttAACATGGTTTTTACATTTCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCAATCAt > 1:2013063/1‑64 (MQ=255) tttAACATGGTTTTTACATTTCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCAATCAt > 1:863035/1‑64 (MQ=255) tttAACATGGTTTTTACATTTCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCAATCATGCTTa > 1:2000167/1‑69 (MQ=255) tAACATGGTTTTTACATTTCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCa > 1:2722448/1‑57 (MQ=255) tAACATGGTTTTTACATTTCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCAATCATGCTTa > 1:401235/1‑67 (MQ=255) tAACATGGTTTTTACATTTCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCAATCATGCTTa > 1:2201160/1‑67 (MQ=255) tAACATGGTTTTTACATTTCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCAATCATGCTTa > 1:1038481/1‑67 (MQ=255) tAACATGGTTTTTACATTTCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCAATCATGCTTa > 1:2523421/1‑67 (MQ=255) tAACATGGTTTTTACATTTCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCAATCATGCTTa > 1:2887560/1‑67 (MQ=255) tAACATGGTTTTTACATTTCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCAATCATGCTTa > 1:2328161/1‑67 (MQ=255) aCATGGTTTTTACATTTCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCAATCATGCTTAAcc < 1:1124976/68‑1 (MQ=255) aCATGGTTTTTACATTTCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCAATCATGCTTAAcc < 1:3315141/68‑1 (MQ=255) gTTTTTACATTTCTCACTGAGCAGTTTTTGAATACaa < 1:2971245/37‑1 (MQ=255) gTTTTTACATTTCTCACTGAGCAGTTTTTGAATACaa < 1:3402938/37‑1 (MQ=255) gTTTTTACATTTCTCACTGAGCAGTTTTTGAATACaa < 1:1494704/37‑1 (MQ=255) gTTTTTACATTTCTCACTGAGCAGTTTTTGAATACaa < 1:1769202/37‑1 (MQ=255) gTTTTTACATTTCTCACTGAGCAGTTTTTGAATACaa < 1:1518669/37‑1 (MQ=255) gTTTTTACATTTCTCACTGAGCAGTTTTTGAATACaa < 1:463469/37‑1 (MQ=255) gTTTTTACATTTCTCACTGAGCAGTTTTTGAATACaa < 1:1704828/37‑1 (MQ=255) gTTTTTACATTTCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCAAt < 1:413065/52‑1 (MQ=255) gTTTTTACATTTCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCAATCATGCTTAACCAGCTc > 1:3106948/1‑68 (MQ=255) gTTTTTACATTTCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCAATCATGCTTAACCAGCTc > 1:776863/1‑68 (MQ=255) gTTTTTACATTTCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCAATCATGCTTAACCAGCTc > 1:2048131/1‑68 (MQ=255) aTTTCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCAATCATGCTTAACCAGCTCGATAACCt < 1:1341441/68‑1 (MQ=255) aTTTCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCAATCATGCTTAACCAGCTCGATAACCt < 1:2436984/68‑1 (MQ=255) aTTTCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCAATCATGCTTAACCAGCTCGATAACCt < 1:1212632/68‑1 (MQ=255) aTTTCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCAATCATGCTTAACCAGCTCGATAACCt < 1:1171312/68‑1 (MQ=255) aTTTCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCAATCATGCTTAACCAGCTCGATAACCt < 1:2231849/68‑1 (MQ=255) aTTTCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCAATCATGCTTAACCAGCTCGATAACCt < 1:3047724/68‑1 (MQ=255) aTTTCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCAATCATGCTTAACCAGCTCGATAACCt < 1:428876/68‑1 (MQ=255) tcACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCAATCATGCTTAACCAGCTCGATAACCTGACgg < 1:744703/68‑1 (MQ=255) tcACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCAATCATGCTTAACCAGCTCGATAACCTGACgg < 1:1382957/68‑1 (MQ=255) tcACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCAATCATGCTTAACCAGCTCGATAACCTGACgg < 1:2639243/68‑1 (MQ=255) tcACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCAATCATGCTTAACCAGCTCGATAACCTGACgg < 1:1266334/68‑1 (MQ=255) tcACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCAATCATGCTTAACCAGCTCGATAACCTGACgg < 1:2645747/68‑1 (MQ=255) tcACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCAATCATGCTTAACCAGCTCGATAACCTGACgg < 1:1977462/68‑1 (MQ=255) tcACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCAATCATGCTTAACCAGCTCGATAACCTGACgg < 1:3251802/68‑1 (MQ=255) tcACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCAATCATGCTTAACCAGCTCGATAACCTGACgg < 1:3225724/68‑1 (MQ=255) cACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCAATCATGCTTAACCAGCTCGATAACCTGACgg < 1:4300/67‑1 (MQ=255) | GCTCTTGCACTACCTTTGCATCACTGGCATGTTTAACATGGTTTTTACATTTCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCAATCATGCTTAACCAGCTCGATAACCTGACGG > minE/2245486‑2245606 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |