| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,245,860 | G→A | 26.9% | P94P (CCG→CCA) | rsd → | stationary phase protein, binds sigma 70 RNA polymerase subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,245,860 | 0 | G | A | 26.9% | 97.1 / 32.3 | 85 | P94P (CCG→CCA) | rsd | stationary phase protein, binds sigma 70 RNA polymerase subunit |
| Reads supporting (aligned to +/- strand): ref base G (15/47); new base A (2/21); total (17/68) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.38e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
TTTATGAGCGCATTCTTCATAAGCTGGAAGGCAACGGGCAACTCGCGCGCGCCGCGAAGATTTGGCCGCAACTCGAAGCCAATACTCAACAGATTATGGATTACTACGATTCCAGTCTGGAAACCGCCATCGAT > minE/2245793‑2245926 | tttATGAGCGCATTCTTCATAAGCTGGAAGGCAACGGGCAACTCGCGCGCGCCGCGAAGATTTGGCCGc > 1:1294454/1‑69 (MQ=255) aTTCTTCATAAGCTGGAAGGCAACGGGCAACTCGCGCGCGCCGCGAAGATTTGGCCGCAACTCGAAGcc < 1:2910045/69‑1 (MQ=255) aTTCTTCATAAGCTGGAAGGCAACGGGCAACTCGCGCGCGCCGCGAAGATTTGGCCGCAACTCGAAGcc < 1:361885/69‑1 (MQ=255) aTTCTTCATAAGCTGGAAGGCAACGGGCAACTCGCGCGCGCCGCGAAGATTTGGCCGCAACTCGAAGcc < 1:2083009/69‑1 (MQ=255) aTTCTTCATAAGCTGGAAGGCAACGGGCAACTCGCGCGCGCCGCGAAGATTTGGCCGCAACTCGAAGcc < 1:1573162/69‑1 (MQ=255) aTTCTTCATAAGCTGGAAGGCAACGGGCAACTCGCGCGCGCCGCGAAGATTTGGCCGCAACTCGAAGcc < 1:354584/69‑1 (MQ=255) aTTCTTCATAAGCTGGAAGGCAACGGGCAACTCGCGCGCGCCGCGAAGATTTGGCCGCAACTCGAAGcc < 1:2147462/69‑1 (MQ=255) aTTCTTCATAAGCTGGAAGGCAACGGGCAACTCGCGCGCGCCGCGAAGATTTGGCCGCAACTCGAAGcc < 1:620046/69‑1 (MQ=255) aTTCTTCATAAGCTGGAAGGCAACGGGCAACTCGCGCGCGCCGCGAAGATTTGGCCGCAACTCGAAGcc < 1:2045084/69‑1 (MQ=255) aTTCTTCATAAGCTGGAAGGCAACGGGCAACTCGCGCGCGCCGCGAAGATTTGGCCGCAACTCGAAGcc < 1:137377/69‑1 (MQ=255) aTTCTTCATAAGCTGGAAGGCAACGGGCAACTCGCGCGCGCCGCGAAGATTTGGCCGCAACTCGAAGcc < 1:3331863/69‑1 (MQ=255) aTTCTTCATAAGCTGGAAGGCAACGGGCAACTCGCGCGCGCCGCGAAGATTTGGCCGCAACTCGAAGcc < 1:797853/69‑1 (MQ=255) aTTCTTCATAAGCTGGAAGGCAACGGGCAACTCGCGCGCGCCGCGAAGATTTGGCCGCAACTCGAAGcc < 1:2683674/69‑1 (MQ=255) aTTCTTCATAAGCTGGAAGGCAACGGGCAACTCGCGCGCGCCGCGAAGATTTGGCCGCAACTCGAAGcc < 1:844490/69‑1 (MQ=255) aTTCTTCATAAGCTGGAAGGCAACGGGCAACTCGCGCGCGCCGCGAAGATTTGGCCGCAACTCGAAGcc < 1:2697854/69‑1 (MQ=255) tcttcATAAGCTGGAAGGCAACGGGCAACTCGCGCGCGCCGCGAAGATTTGGCCGCAACTCGAAGCCaa < 1:704327/69‑1 (MQ=255) tcttcATAAGCTGGAAGGCAACGGGCAACTCGCGCGCGCCGCGAAGATTTGGCCGCAACTCGAAGCCaa < 1:1276098/69‑1 (MQ=255) cttcATAAGCTGGAAGGCAACGGGCAACTCGCGCGCGCCGCGAAGATTTGGCCGCAACTCGAAGCCaa > 1:509588/1‑68 (MQ=255) cttcATAAGCTGGAAGGCAACGGGCAACTCGCGCGCGCCGCGAAGATTTGGCCGCAACTCGAAGCCaa > 1:1808073/1‑68 (MQ=255) cttcATAAGCTGGAAGGCAACGGGCAACTCGCGCGCGCCGCGAAGATTTGGCCGCAACTCGAAGCCaa > 1:3399408/1‑68 (MQ=255) cttcATAAGCTGGAAGGCAACGGGCAACTCGCGCGCGCCGCGAAGATTTGGCCGCAACTCGAAGCCaa > 1:339093/1‑68 (MQ=255) cttcATAAGCTGGAAGGCAACGGGCAACTCGCGCGCGCCGCGAAGATTTGGCCGCAACTCGAAGCCAAt > 1:46943/1‑69 (MQ=255) cttcATAAGCTGGAAGGCAACGGGCAACTCGCGCGCGCCGCGAAGATTTGGCCGCAACTCGAAGCCAAt > 1:1339961/1‑69 (MQ=255) cttcATAAGCTGGAAGGCAACGGGCAACTCGCGCGCGCCGCGAAGATTTGGCCGCAACTCGAAGCCAAt > 1:1602353/1‑69 (MQ=255) tcATAAGCTGGAAGGCAACGGGCAACTCGCGCGCGCCGCGAAGATTTGGCCGCAACTCGAAGCCAATa < 1:1580552/68‑1 (MQ=255) cATAAGCTGGAAGGCAACGGGCAACTCGCGCGCGCCGCGAAGATTTGGCCGCAACTCGAAGcc < 1:3044191/63‑1 (MQ=255) cATAAGCTGGAAGGCAACGGGCAACTCGCGCGCGCCGCGAAGATTTGGCCGCAACTCGAAGcc < 1:1621917/63‑1 (MQ=255) cATAAGCTGGAAGGCAACGGGCAACTCGCGCGCGCCGCGAAGATTTGGCCGCAACTCGAAGcc < 1:991781/63‑1 (MQ=255) cATAAGCTGGAAGGCAACGGGCAACTCGCGCGCGCCGCGAAGATTTGGCCGCAACTCGAAGcc < 1:3416412/63‑1 (MQ=255) cATAAGCTGGAAGGCAACGGGCAACTCGCGCGCGCCGCGAAGATTTGGCCGCAACTCGAAGcc < 1:1518470/63‑1 (MQ=255) tAAGCTGGAAGGCAACGGGCAACTCGCGCGCGCCGCGAAGATTTGGCCGCAACTCGAAGCCAATACTc < 1:1097499/68‑1 (MQ=255) ggAAGGCAACGGGCAACTCGCGCGCGCCGCGAAGATTTGGCCGCAACTCGAAGCCAATACTCAACAGAt < 1:1516066/69‑1 (MQ=255) ggAAGGCAACGGGCAACTCGCGCGCGCCGCGAAGATTTGGCCGCAACTCGAAGCCAATACTCAACAGAt < 1:701868/69‑1 (MQ=255) ggAAGGCAACGGGCAACTCGCGCGCGCCGCGAAGATTTGGCCGCAACTCGAAGCCAATACTCAACAGAt < 1:3378286/69‑1 (MQ=255) ggAAGGCAACGGGCAACTCGCGCGCGCCGCGAAGATTTGGCCGCAACTCGAAGCCAATACTCAACAGAt < 1:1977255/69‑1 (MQ=255) ggAAGGCAACGGGCAACTCGCGCGCGCCGCGAAGATTTGGCCGCAACTCGAAGCCAATACTCAACAGAt < 1:2863413/69‑1 (MQ=255) gAAGGCAATGGGCAACTCGCACGCGCCGCGAAGATTTGGCCACAACTCGAAGCCAATACTCAACAGAtt > 1:537171/1‑69 (MQ=255) gAAGGCAATGGGCAACTCGCACGCGCCGCGAAGATTTGGCCACAACTCGAAGCCAATACTCAACAGAtt > 1:528807/1‑69 (MQ=255) gCAACGGGCAACTCGCGCGCGCCGCGAAGATTTGGCCGCAACTCGAAGCCAATACTCAACAGATTATgg < 1:697308/69‑1 (MQ=255) gCAACGGGCAACTCGCGCGCGCCGCGAAGATTTGGCCGCAACTCGAAGCCAATACTCAACAGATTATgg < 1:235670/69‑1 (MQ=255) gCAACGGGCAACTCGCGCGCGCCGCGAAGATTTGGCCGCAACTCGAAGCCAATACTCAACAGATTATgg < 1:3131080/69‑1 (MQ=255) aaCGGGCAACTCGCGCGCGCCGCGAAGATTTGGCCGCAACTCGAAGCCAATACTCAACAGATTATGGAt < 1:393837/69‑1 (MQ=255) aaCGGGCAACTCGCGCGCGCCGCGAAGATTTGGCCGCAACTCGAAGCCAATACTCAACAGATTATGGAt < 1:820154/69‑1 (MQ=255) aaCGGGCAACTCGCGCGCGCCGCGAAGATTTGGCCGCAACTCGAAGCCAATACTCAACAGATTATGGAt < 1:1141875/69‑1 (MQ=255) aaCGGGCAACTCGCGCGCGCCGCGAAGATTTGGCCGCAACTCGAAGCCAATACTCAACAGATTATGGAt < 1:1310593/69‑1 (MQ=255) aaCGGGCAACTCGCGCGCGCCGCGAAGATTTGGCCGCAACTCGAAGCCAATACTCAACAGATTATGGAt < 1:184982/69‑1 (MQ=255) aaCGGGCAACTCGCGCGCGCCGCGAAGATTTGGCCGCAACTCGAAGCCAATACTCAACAGATTATGGAt < 1:2509017/69‑1 (MQ=255) aaCGGGCAACTCGCGCGCGCCGCGAAGATTTGGCCGCAACTCGAAGCCAATACTCAACAGATTATGGAt < 1:2616083/69‑1 (MQ=255) atgggCAACTCGCACGCGCCGCGAAGATTTGGCCAGAACTCGAAGCCAATACTCAACAGATTATGGAtg < 1:2091981/67‑2 (MQ=255) atgggCAACTCGCACGCGCCGCGAAGATTTGGCCACAACTCGAAGCCAATACTCAACAGATTATGGAtg < 1:1200587/67‑2 (MQ=255) atgggCAACTCGCACGCGCCGCGAAGATTTGGCCACAACTCGAAGCCAATACTCAACAGATTATGGAtg < 1:862495/67‑2 (MQ=255) atgggCAACTCGCACGCGCCGCGAAGATTTGGCCACAACTCGAAGCCAATACTCAACAGATTATGGAtg < 1:1365849/67‑2 (MQ=255) atgggCAACTCGCACGCGCCGCGAAGATTTGGCCACAACTCGAAGCCAATACTCAACAGATTATGGAtg < 1:1369644/67‑2 (MQ=255) atgggCAACTCGCACGCGCCGCGAAGATTTGGCCACAACTCGAAGCCAATACTCAACAGATTATGGAtg < 1:142217/67‑2 (MQ=255) atgggCAACTCGCACGCGCCGCGAAGATTTGGCCACAACTCGAAGCCAATACTCAACAGATTATGGAtg < 1:465063/67‑2 (MQ=255) atgggCAACTCGCACGCGCCGCGAAGATTTGGCCACAACTCGAAGCCAATACTCAACAGATTATGGAtg < 1:434454/67‑2 (MQ=255) atgggCAACTCGCACGCGCCGCGAAGATTTGGCCACAACTCGAAGCCAATACTCAACAGATTATGGAtg < 1:3377638/67‑2 (MQ=255) atgggCAACTCGCACGCGCCGCGAAGATTTGGCCACAACTCGAAGCCAATACTCAACAGATTATGGAtg < 1:3366378/67‑2 (MQ=255) atgggCAACTCGCACGCGCCGCGAAGATTTGGCCACAACTCGAAGCCAATACTCAACAGATTATGGAtg < 1:3166607/67‑2 (MQ=255) atgggCAACTCGCACGCGCCGCGAAGATTTGGCCACAACTCGAAGCCAATACTCAACAGATTATGGAtg < 1:2863901/67‑2 (MQ=255) atgggCAACTCGCACGCGCCGCGAAGATTTGGCCACAACTCGAAGCCAATACTCAACAGATTATGGAtg < 1:2698795/67‑2 (MQ=255) atgggCAACTCGCACGCGCCGCGAAGATTTGGCCACAACTCGAAGCCAATACTCAACAGATTATGGAtg < 1:2901707/67‑2 (MQ=255) atgggCAACTCGCACGCGCCGCGAAGATTTGGCCACAACTCGAAGCCAATACTCAACAGATTATGGAtg < 1:3054285/67‑2 (MQ=255) atgggCAACTCGCACGCGCCGCGAAGATTTGGCCACAACTCGAAGCCAATACTCAACAGATTATGGAtg < 1:2877851/67‑2 (MQ=255) atgggCAACTCGCACGCGCCGCGAAGATTTGGCCACAACTCGAAGCCAATACTCAACAGATTATGGAtg < 1:3292507/67‑2 (MQ=255) atgggCAACTCGCACGCGCCGCGAAGATTTGGCCACAACTCGAAGCCAATACTCAACAGATTATGGAtg < 1:3313260/67‑2 (MQ=255) tgggCAACTCGCACGCGCCGCGAAGATTTGGCCACAACTCGAAGCCAATACTCAACAGATTATGGAtg < 1:3349475/67‑2 (MQ=255) tgggCAACTCGCACGCGCCGCGAAGATTTGGCCACAACTCGAAGCCAATACTCAACAGATTATGGAtg < 1:2493562/67‑2 (MQ=255) tgggCAACTCGCACGCGCCGCGAAGATTTGGCCACAACTCGAAGCCAATACTCAACAGATTATGGAtg < 1:698077/67‑2 (MQ=255) cAACTCGCGCGCGCCGCGAAGATTTGGCCGCAACTCGAAGCCAATACTCAACAGATTATGGATtactac > 1:2046904/1‑69 (MQ=255) cAACTCGCGCGCGCCGCGAAGATTTGGCCGCAACTCGAAGCCAATACTCAACAGATTATGGATtactac > 1:1663154/1‑69 (MQ=255) cTCGCGCGCGCCGCGAAGATTTGGCCGCAACTCGAAGCCAATACTCAACAGATTATGGATTACTACGAt < 1:1420674/69‑1 (MQ=255) cTCGCGCGCGCCGCGAAGATTTGGCCGCAACTCGAAGCCAATACTCAACAGATTATGGATTACTACGAt < 1:3329908/69‑1 (MQ=255) cgcgcgcgCCGCGAAGATTTGGCCGCAACTCGAAGCCaa < 1:355347/39‑1 (MQ=255) cgcgcgcgCCGCGAAGATTTGGCCGCAACTCGAAGCCaa > 1:3405597/1‑39 (MQ=255) cgcgcgcgCCGCGAAGATTTGGCCGCAACTCGAAGCCaa > 1:268527/1‑39 (MQ=255) cgcgcgcgCCGCGAAGATTTGGCCGCAACTCGAAGCCaa > 1:2287510/1‑39 (MQ=255) cgcgcgcgCCGCGAAGATTTGGCCGCAACTCGAAGCCAATACTCAACAGATTATGGATTACTACGAt > 1:1253432/1‑67 (MQ=255) cgcgcgcgCCGCGAAGATTTGGCCGCAACTCGAAGCCAATACTCAACAGATTATGGATTACTACGAt > 1:2979544/1‑67 (MQ=255) ggCAACTCGAAGCCAATACTCAACAGATTATGGATTACTACGATTCCAGTCTGGAAACCGCCATCGAt < 1:2864566/67‑1 (MQ=255) cGCAACTCGAAGCCTATACTCAACAGATTATGGATTACTACGATTCCAGTCTGGAAACCGCCATCGAt < 1:2180774/68‑1 (MQ=255) cGCAACTCGAAGCCAATACTCAACAGATTATGGATTACTACGATTCCAGTCTGGAAACCGCCATCGAt < 1:1580766/68‑1 (MQ=255) cGCAACTCGAAGCCAATACTCAACAGATTATGGATTACTACGATTCCAGTCTGGAAACCGCCATCGAt < 1:1254776/68‑1 (MQ=255) cGCAACTCGAAGCCAATACTCAACAGATTATGGATTACTACGATTCCAGTCTGGAAACCGCCATCGAt < 1:1781155/68‑1 (MQ=255) cGCAACTCGAAGCCAATACTCAACAGATTATGGATTACTACGATTCCAGTCTGGAAACCGCCATCGAt < 1:201212/68‑1 (MQ=255) cGCAACTCGAAGCCAATACTCAACAGATTATGGATTACTACGATTCCAGTCTGGAAACCGCCATCGAt < 1:883362/68‑1 (MQ=255) | TTTATGAGCGCATTCTTCATAAGCTGGAAGGCAACGGGCAACTCGCGCGCGCCGCGAAGATTTGGCCGCAACTCGAAGCCAATACTCAACAGATTATGGATTACTACGATTCCAGTCTGGAAACCGCCATCGAT > minE/2245793‑2245926 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |