| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,246,389 | T→C | 21.5% | Y34Y (TAT→TAC) | thiC → | thiamin (pyrimidine moiety) biosynthesis protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,246,389 | 0 | T | C | 21.5% | 78.9 / 15.2 | 60 | Y34Y (TAT→TAC) | thiC | thiamin (pyrimidine moiety) biosynthesis protein |
| Reads supporting (aligned to +/- strand): ref base T (19/28); new base C (5/8); total (24/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.18e-01 | |||||||||||
CGCCCGGGCCCAACATTTTATCGACACCCTGGAAGGCACCGCCTTTCCCAACTCAAAACGCATTTATATCACTGGCACACACCCCGGCGTGCGCGTGCCGATGCGTGAGATCCAGCTTAGCCCGACGCTAATT > minE/2246323‑2246455 | cgcCCGGGCCCAACATTTTATCGACACCCTGGATGGCACCGCCTTTCCCAACTCAAAACGCATTTatat < 1:354788/69‑1 (MQ=255)cgcCCGGGCCCAACATTTTATCGACACCCTGGAAGGCACCGCCTTTCCCAACTCAAAACGCATTTatat < 1:2689821/69‑1 (MQ=255)cgcCCGGGCCCAACATTTTATCGACACCCTGGAAGGCACCGCCTTTCCCAACTCAAAACGCATTTatat < 1:480319/69‑1 (MQ=255)cgcCCGGGCCCAACATTTTATCGACACCCTGGAAGGCACCGCCTTTCCCAACTCAAAACGCATTTatat < 1:2390374/69‑1 (MQ=255)cgcCCGGGCCCAACATTTTATCGACACCCTGGAAGGCACCGCCTTTCCCAACTCAAAACGCATTTatat < 1:173963/69‑1 (MQ=255) gcCCGGGCCCAACATTTTATCGACACCCTGGAAGGCACCGCCTTTCCCAACTCAAAACGCATTTatat < 1:2872772/68‑1 (MQ=255) gcCCGGGCCCAACATTTTATCGACACCCTGGAAGGCACCGCCTTTCCCAACTCAAAACGCATTTatat < 1:1223675/68‑1 (MQ=255) gcCCGGGCCCAACATTTTATCGACACCCTGGAAGGCACCGCCTTTCCCAACTCAAAACGCATTTATATc < 1:3094855/69‑1 (MQ=255) gcCCGGGCCCAACATTTTATCGACACCCTGGAAGGCACCGCCTTTCCCAACTCAAAACGCATTTATATc < 1:2872593/69‑1 (MQ=255) gcCCGGGCCCAACATTTTATCGACACCCTGGAAGGCACCGCCTTTCCCAACTCAAAACGCATTTATATc < 1:832325/69‑1 (MQ=255) gcCCGGGCCCAACATTTTATCGACACCCTGGAAGGCACCGCCTTTCCCAACTCAAAACCCATTTatat < 1:1453618/68‑1 (MQ=255) cccGGGCCCAACATTTTATCGACACCCTGGAAGGCACCGCCTTTCCCAACTCAAAACGCATTTATATc < 1:2390640/68‑1 (MQ=255) cccGGGCCCAACATTTTATCGACACCCTGGAAGGCACCGCCTTTCCCAACTCAAAACGCATTTATATc < 1:80887/68‑1 (MQ=255) ccaggCCCAACATTTTATCGATACCCTGGAAGGCTCCGCCTTTCGCAACTCAAAACGCATTTACATCAc > 1:684427/4‑69 (MQ=255) ccaggCCCAACATTTTATCGATACCCTGGAAGGCTCCGCCTTTCCCAACTCAAAACGCATTTACATCAc > 1:1687685/4‑69 (MQ=255) ccaggCCCAACATTTTATCGATACCCTGGAAGGCTCCGCCTTTCCCAACTCAAAACGCATTTACATCAc > 1:3256711/4‑69 (MQ=255) ccaggCCCAACATTTTATCGATACCCTGGAAGGCTCCGCCTTTCCCAACTCAAAACGCATTTACATCAc > 1:428138/4‑69 (MQ=255) ccaggCCCAACATTTTATCGATACCCTGGAAGGCTCCGCCTTTCCCAACTCAAAACGCATTTACATCAc > 1:1354648/4‑69 (MQ=255) cccAACATTTTATCGACACCCTGGAAGGCACCGCCTTTCCCAACTCAAAACGCATTTATATCACTGGca < 1:997673/69‑1 (MQ=255) cccAACATTTTATCGACACCCTGGAAGGCACCGCCTTTCCCAACTCAAAACGCATTTATATCACTGGca < 1:1174987/69‑1 (MQ=255) cccAACATTTTATCGACACCCTGGAAGGCACCGCCTTTCCCAACTCAAAACGCATTTATATCACTGGca < 1:862267/69‑1 (MQ=255) cccAACATTTTATCGACACCCTGGAAGGCACCGCCTTTCCCAACTCAAAACGCATTTATATCACTGGca < 1:428978/69‑1 (MQ=255) cccAACATTTTATCGACACCCTGGAAGGCACCGCCTTTCCCAACTCAAAACGCATTTATATCACTGGca < 1:2691386/69‑1 (MQ=255) cccAACATTTTATCGACACCCTGGAAGGCACCGCCTTTCCCAACTCAAAACGCATTTATATCACTGGca < 1:645550/69‑1 (MQ=255) cccAACATTTTATCGACACCCTGGAAGGCACCGCCTTTCCCAACTCAAAACGCATTTATATCACTGGca < 1:162897/69‑1 (MQ=255) cccAACATTTTATCGACACCCTGGAAGGCACCGCCTTTCCCAACTCAAAACGCATTTATATCACTGGca < 1:2048011/69‑1 (MQ=255) cccAACATTTTATCGACACCCTGGAAGGCACCGCCTTTCCCAACTCAAAACGCATTTATATCACTGGca < 1:1883746/69‑1 (MQ=255) cccAACATTTTATCGACACCCTGGAAGGCACCGCCTTTCCCAACTCAAAACGCATTTATATCACTGGca < 1:1914041/69‑1 (MQ=255) cccAACATTTTATCGACACCCTGGAAGGCACCGCCTTTCCCAACTCAAAACGCATTTATATCACTGGca < 1:2003605/69‑1 (MQ=255) cccAACATTTTATCGACACCCTGGAAGGCACCGCCTTTCCCAACTCAAAACGCATTTATATCACTGGca < 1:1522036/69‑1 (MQ=255) ttATCGACACCCTGGAAGGCACCGCCTTTCCCAACTCAAAACGCATTTATATCACTGGCacacac > 1:729691/1‑65 (MQ=255) ttATCGACACCCTGGAAGGCACCGCCTTTCCCAACTCAAAACGCATTTATATCACTGGCacacac > 1:2923148/1‑65 (MQ=255) ttATCGACACCCTGGAAGGCACCGCCTTTCCCAACTCAAAACGCATTTATATCACTGGCacacac > 1:2004622/1‑65 (MQ=255) ttATCGACACCCTGGAAGGCACCGCCTTTCCCAACTCAAAACGCATTTATATCACTGGCacacac > 1:1968290/1‑65 (MQ=255) aaGGCACCGCCTTTCCCAACTCAAAACGCATTTATATc < 1:1680674/38‑1 (MQ=255) aGGCACCGCCTTTCCCAACTCAAAACGCATTTATATCACTGGCACACACCCCGGCGTGCGCGTGCCGa < 1:164012/68‑1 (MQ=255) cACCGCCTTTCCCAACTCAAAACGCATTTATATCACTGGCACACACCCCGGCGTGCGCGTGCCGATGc > 1:2704637/1‑68 (MQ=255) cACCGCCTTTCCCAACTCAAAACGCATTTATATCACTGGCACACACCCCGGCGTGCGCGTGCCGATGc > 1:3154831/1‑68 (MQ=255) cACCGCCTTTCCCAACTCAAAACGCATTTATATCACTGGCACACACCCCGGCGTGCGCGTGCCGATGc > 1:3058529/1‑68 (MQ=255) cACCGCCTTTCCCAACTCAAAACGCATTTATATCACTGGCACACACCCCGGCGTGCGCGTGCCGATGc > 1:2871024/1‑68 (MQ=255) cACCGCCTTTCCCAACTCAAAACGCATTTATATCACTGGCACACACCCCGGCGTGCGCGTGCCGATGc > 1:1269771/1‑68 (MQ=255) cACCGCCTTTCCCAACTCAAAACGCATTTATATCACTGGCACACACCCCGGCGTGCGCGTGCCGATGc > 1:130638/1‑68 (MQ=255) cACCGCCTTTCCCAACTCAAAACGCATTTATATCACTGGCACACACCCCGGCGTGCGCGTGCCGATGc > 1:2392635/1‑68 (MQ=255) cACCGCCTTTCCCAACTCAAAACGCATTTATATCACTGGCACACACCCCGGCGTGCGCGTGCCGATGc > 1:2277425/1‑68 (MQ=255) cACCGCCTTTCCCAACTCAAAACGCATTTATATCACTGGCACACACCCCGGCGTGCCCGTGCCGATg > 1:2328580/1‑67 (MQ=255) cACCGACTTTCCCAACTCAAAACGCATTTATATCACTGGcacaca > 1:1159919/1‑45 (MQ=255) aCCGCCTTTCCCAACTCAAAACGCATTTATATCACTGGCACACACCCCGGCGTGCGCGTGCCGATGc > 1:467066/1‑67 (MQ=255) aCCGCCTTTCCCAACTCAAAACGCATTTATATCACTGGCACACACCCCGGCGTGCGCGTGCCGATGc > 1:3042947/1‑67 (MQ=255) aCCGCCTTTCCCAACTCAAAACGCATTTATATCACTGGCACACACCCCGGCGTGCGCGTGCCGATGc > 1:2307237/1‑67 (MQ=255) aCCGCCTTTCCCAACTCAAAACGCATTTATATCACTGGCACACACCCCGGCGTGCGCGTGCCGATGc > 1:2307088/1‑67 (MQ=255) aCCGCCTTTCCCAACTCAAAACGCATTTATATCACTGGCACACACCCCGGCGTGCGCGTGCCGATGc > 1:3201951/1‑67 (MQ=255) cGCCTTTCCCAACTCAAAACGCATTTATATCACTGGca < 1:1476919/38‑1 (MQ=255) gCCTTTCCCAACTCAAAACGCATTTATATCACTGGCACACACCCCGGCGTGCGCGTGCCGATGCGTgag < 1:403163/69‑1 (MQ=255) gCCTTTCCCAACTCAAAACGCATTTATATCACTGGCACACACCCCGGCGTGCGCGTGCCGATGCGTgag < 1:26578/69‑1 (MQ=255) gCCTTTCCCAACTCAAAACGCATTTATATCACTGGCACACACCCCGGCGTGCGCGTGCCGATGCGTgag < 1:1810265/69‑1 (MQ=255) gCCTTTCCCAACTCAAAACGCATTTATATCACTGGCACACACCCCGGCGTGCGCGTGCCGATGCGTgag < 1:2959396/69‑1 (MQ=255) gCCTTTCCCAACTCAAAACGCATTTATATCACTGGCACACACCCCGGCGTGCGCGTGCCGATGCGTgag < 1:254446/69‑1 (MQ=255) ccTTTCCCAACTCAAAACGCATTTACATCACTGCCACACAACCCGGCGTGCGCGTGCCGATGCGTga < 1:3430351/67‑1 (MQ=255) ccTTTCCCAACTCAAAACGCATTTACATCACTGCCACACAACCCGGCGTGCGCGTGCCGATGCGTga < 1:814857/67‑1 (MQ=255) ccTTTCCCAACTCAAAACGCATTTACATCACTGCCACACAACCCGGCGTGCGCGTGCCGATGCGTga < 1:2290563/67‑1 (MQ=255) ccTTTCCCAACTCAAAACGCATTTACATCACTGCCACACAACCCGGCGTGCGCGTGCCGATGCGTga < 1:718876/67‑1 (MQ=255) ccTTTCCCAACTCAAAACGCATTTACATCACTGCCACACAACCCGGCGTGCGCGTGCCGATGCGTga < 1:2414002/67‑1 (MQ=255) ccTTTCCCAACTCAAAACGCATTTACATCACTGCCACACAACCCGGCGTGCGCGTGCCGATGCGTga < 1:1088649/67‑1 (MQ=255) ccTTTCCCAACTCAAAACGCATTTACATCACTGCCACACAACCCGGCGTGCGCGTGCCGATGCGTga < 1:3314064/67‑1 (MQ=255) ccTTTCCCAACTCAAAACGCATTTACATCACTGCCACACAACCCGGCGTGCGCGTGCCGATGCGTga < 1:350576/67‑1 (MQ=255) aCTCAAAACGCATTTATATCACTGGCACACACCCCGGCGTGCGCGTGCCGATGCGTGAGATCCAGCTTa < 1:2855071/69‑1 (MQ=255) aCTCAAAACGCATTTATATCACTGGCACACACCCCGGCGTGCGCGTGCCGATGCGTGAGATCCAGCTTa < 1:2048770/69‑1 (MQ=255) aCTCAAAACGCATTTATATCACTGGCACACACCCCGGCGTGCGCGTGCCGATGCGTGAGATCCAGCTTa < 1:118788/69‑1 (MQ=255) tataTCACTGGCACACACCCCGGCGTGCGCGTGCCGATGCGTGAGATCCAGCTTAGCCCGACGCTAAtt > 1:3345218/1‑69 (MQ=255) tataTCACTGGCACACACCCCGGCGTGCGCGTGCCGATGCGTGAGATCCAGCTTAGCCCGACGCTAAtt > 1:1091433/1‑69 (MQ=255) tataTCACTGGCACACACCCCGGCGTGCGCGTGCCGATGCGTGAGATCCAGCTTAGCCCGACGCTAAt > 1:2302154/1‑68 (MQ=255) tataTCACTGGCACACACCCCGGCGTGCGCGTGCCGATGCGTGAGATCCAGCTTAGCCCGACGCTAAt > 1:3174156/1‑68 (MQ=255) | CGCCCGGGCCCAACATTTTATCGACACCCTGGAAGGCACCGCCTTTCCCAACTCAAAACGCATTTATATCACTGGCACACACCCCGGCGTGCGCGTGCCGATGCGTGAGATCCAGCTTAGCCCGACGCTAATT > minE/2246323‑2246455 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |