| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,246,542 | T→C | 20.9% | I85I (ATT→ATC) | thiC → | thiamin (pyrimidine moiety) biosynthesis protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,246,542 | 0 | T | C | 20.9% | 133.5 / 25.8 | 95 | I85I (ATT→ATC) | thiC | thiamin (pyrimidine moiety) biosynthesis protein |
| Reads supporting (aligned to +/- strand): ref base T (54/21); new base C (8/12); total (62/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.56e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 | |||||||||||
GCAGTACGAAGAAAACGAAGCGATTCCGGTCTACGACACCTCCGGCCCGTATGGTGATCCGCAGATTGCCATTAACGTGCAGCAAGGGCTGGCAAAACTACGCCAGCCGTGGATCGATGCGCGCGGCG > minE/2246476‑2246603 | gCAGTACGAAGAAAACGAAGCGATTCCGGTCTACGACACCTCCGGCCCGTATGGTGATCCGCAGATTg > 1:3337803/1‑68 (MQ=255)gCAGTACGAAGAAAACGAAGCGATTCCGGTCTACGACACCTCCGGCCCGTATGGTGATCCGCAGATTg > 1:2683070/1‑68 (MQ=255)gCAGTACGAAGAAAACGAAGCGATTCCGGTCTACGACACCTCCGGCCCGTATGGTGATCCGCAGATTg > 1:2248704/1‑68 (MQ=255)gCAGTACGAAGAAAACGAAGCGATTCCGGTCTACGACACCTCCGGCCCGTATGGTGATCCGCAGATTg > 1:1569090/1‑68 (MQ=255) aCGAAGAAAACGAAGCGATTCCGGTCTACGACACCTCCGGTCCGTATGGCGATCCGCAGATCGCCATTa < 1:1079652/69‑1 (MQ=255) aCGAAGAAAACGAAGCGATTCCGGTCTACGACACCTCCGGTCCGTATGGCGATCCGCAGATCGCCATTa < 1:3398720/69‑1 (MQ=255) aCGAAGAAAACGAAGCGATTCCGGTCTACGACACCTCCGGTCCGTATGGCGATCCGCAGATCGCCATTa < 1:3140486/69‑1 (MQ=255) aCGAAGAAAACGAAGCGATTCCGGTCTACGACACCTCCGGTCCGTATGGCGATCCGCAGATCGCCATTa < 1:2627979/69‑1 (MQ=255) aCGAAGAAAACGAAGCGATTCCGGTCTACGACACCTCCGGTCCGTATGGCGATCCGCAGATCGCCATTa < 1:2141788/69‑1 (MQ=255) aCGAAGAAAACGAAGCGATTCCGGTCTACGACACCTCCGGTCCGTATGGCGATCCGCAGATCGCCATTa < 1:207332/69‑1 (MQ=255) aCGAAGAAAACGAAGCGATTCCGGTCTACGACACCTCCGGTCCGTATGGCGATCCGCAGATCGCCATTa < 1:1928998/69‑1 (MQ=255) aCGAAGAAAACGAAGCGATTCCGGTCTACGACACCTCCGGTCCGTATGGCGATCCGCAGATCGCCATTa < 1:171432/69‑1 (MQ=255) aCGAAGAAAACGAAGCGATTCCGGTCTACGACACCTCCGGTCCGTATGGCGATCCGCAGATCGCCATTa < 1:1291207/69‑1 (MQ=255) aCGAAGAAAACGAAGCGATTCCGGTCTACGACACCTCCGGTCCGTATGGCGATCCGCAGATCGCCATTa < 1:1330964/69‑1 (MQ=255) cGAAGAAAACGAAGCGATTCCGGTCTACGACACCTCCGGTCCGTATGGCGATCCGCAGATCGCCATTaa > 1:2671465/1‑69 (MQ=255) cGAAGAAAACGAAGCGATTCCGGTCTACGACACCTCCGGTCCGTATGGCGATCCGCAGATCGCCATTaa > 1:232537/1‑69 (MQ=255) cGAAGAAAACGAAGCGATTCCGGTCTACGACACCTCCGGTCCGTATGGCGATCCGCAGATCGCCATTaa > 1:1773763/1‑69 (MQ=255) cGAAGAAAACGAAGCGATTCCGGTCTACGACACCTCCGGTCCGTATGGCGATCCGCAGATCGCCATTaa > 1:1771395/1‑69 (MQ=255) cGAAGAAAACGAAGCGATTCCGGTCTACGACACCTCCGGTCCGTATGGCGATCCGCAGATCGCCATTaa > 1:3079204/1‑69 (MQ=255) cGAAGAAAACGAAGCGATTCCGGTCTACGACACCTCCGGTCCGTATGGCGATCCGCAGATCGCCATTaa > 1:1211756/1‑69 (MQ=255) cGAAGAAAACGAAGCGATTCCGGTCTACGACACCTCCGGTCCGTATGGCGATCCGCAGATCGCCATTaa > 1:1510760/1‑69 (MQ=255) cGAAGAAAACGAAGCGATTCCGGTCTACGACACCTCCGGTCCGTATGGCGATCCGCAGATCGCCATTaa > 1:1535707/1‑69 (MQ=255) cGAAGAAAACGAAGCGATTCCGGTCTACGACACCTCCGGTCCGTATGGCGATCCGCAGATCGCCATTa < 1:534856/68‑1 (MQ=255) cGAAGAAAACGAAGCGATTCCGGTCTACGACACCTCCGGTCCGTATGGCGATCCGCAGATCGCCATTa < 1:1177248/68‑1 (MQ=255) cGAAGAAAACGAAGCGATTCCGGTCTACGACACCTCCGGCCCGTATGGTGATCCGCAGATTGCCATTaa > 1:837904/1‑69 (MQ=255) cGAAGAAAACGAAGCGATTCCGGTCTACGACACCTCCGGCCCGTATGGTGATCCGCAGATTGCCATTaa > 1:280636/1‑69 (MQ=255) cGAAGAAAACGAAGCGATTCCGGTCTACGACACCTCCGGCCCGTATGGTGATCCGCAGATTGCCATTaa > 1:2810329/1‑69 (MQ=255) cGAAGAAAACGAAGCGATTCCGGTCTACGACACCTCCGGCCCGTATGGTGATCCGCAGATTGCCATTaa > 1:298021/1‑69 (MQ=255) cGAAGAAAACGAAGCGATTCCGGTCTACGACACCTCCGGCCCGTATGGTGATCCGCAGATTGCCATTaa > 1:1939589/1‑69 (MQ=255) gCGATTCCGGTCTACGACACCTCCGGCCCGTATGGTGATCCGCAGATTGCCATTAACGTGCAGCAAggg > 1:1319477/1‑69 (MQ=255) gCGATTCCGGTCTACGACACCTCCGGCCCGTATGGTGATCCGCAGATTGCCATTAACGTGCAGCAAggg > 1:2567655/1‑69 (MQ=255) gCGATTCCGGTCTACGACACCTCCGGCCCGTATGGTGATCCGCAGATTGCCATTAACGTGCAGCAAggg > 1:2448481/1‑69 (MQ=255) gCGATTCCGGTCTACGACACCTCCGGCCCGTATGGTGATCCGCAGATTGCCATTAACGTGCAGCAAggg > 1:1391099/1‑69 (MQ=255) gCGATTCCGGTCTACGACACCTCCGGCCCGTATGGTGATCCGCAGATTGCCATTAACGTGCAGCAAggg > 1:1841543/1‑69 (MQ=255) gCGATTCCGGTCTACGACACCTCCGGCCCGTATGGTGATCCGCAGATTGCCATTAACGTGCAGCAAggg > 1:858596/1‑69 (MQ=255) tACGACACCTCCGGCCCGTATGGTGATCCGCAGATTGCCATTAACGTGCAGCAAGGGCTGGCAAAACTa > 1:2690262/1‑69 (MQ=255) tACGACACCTCCGGCCCGTATGGTGATCCGCAGATTGCCATTAACGTGCAGCAAGGGCTGGCAAAACTa > 1:262835/1‑69 (MQ=255) tACGACACCTCCGGCCCGTATGGTGATCCGCAGATTGCCATTAACGTGCAGCAAGGGCTGGCAAAACTa > 1:3349629/1‑69 (MQ=255) tACGACACCTCCGGCCCGTATGGTGATCCGCAGATTGCCATTAACGTGCAGCAAGGGCTGGCAAAACTa > 1:2982035/1‑69 (MQ=255) tACGACACCTCCGGCCCGTATGGTGATCCGCAGATTGCCATTAACGTGCAGCAAGGGCTGGCAAAACTa > 1:1633548/1‑69 (MQ=255) tACGACACCTCCGGCCCGTATGGTGATCCGCAGATTGCCATTAACGTGCAGCAAGGGCTGGCAAAACTa > 1:3097816/1‑69 (MQ=255) tACGACACCTCCGGCCCGTATGGTGATCCGCAGATTGCCATTAACGTGCAGCAAGGGCTGGCAAAACTa > 1:3099152/1‑69 (MQ=255) aTGGTGATCCGCAGATTGCCATTAACGTGCAGCAAGGGCTGGCAAAACTACGCCAGCCGTGGATCg > 1:2693108/1‑66 (MQ=255) aTGGTGATCCGCAGATTGCCATTAACGTGCAGCAAGGGCTGGCAAAACTACGCCAGCCGTGGATCGATg > 1:2080443/1‑69 (MQ=255) aTGGTGATCCGCAGATTGCCATTAACGTGCAGCAAGGGCTGGCAAAACTACGCCAGCCGTGGATCGATg > 1:492246/1‑69 (MQ=255) aTGGTGATCCGCAGATTGCCATTAACGTGCAGCAAGGGCTGGCAAAACTACGCCAGCCGTGGATCGATg > 1:488356/1‑69 (MQ=255) tGGTGATCCGCAGATTGCCATTAACGTGCAGCAAGGGCTGGCAAAACTACGCCAGCCGTGGATCGATgc > 1:623070/1‑69 (MQ=255) gTGATCCGCAGATTGCCATTAACGTGCAGCAAGGGCTGGCAAAACTACGCCAGCCGTGGATCGATgcgc > 1:2819381/1‑69 (MQ=255) gTGATCCGCAGATTGCCATTAACGTGCAGCAAGGGCTGGCAAAACTACGCCAGCCGTGGATCGATgcgc > 1:3030886/1‑69 (MQ=255) gTGATCCGCAGATTGCCATTAACGTGCAGCAAGGGCTGGCAAAACTACGCCAGCCGTGGATCGATgcgc > 1:998535/1‑69 (MQ=255) gTGATCCGCAGATTGCCATTAACGTGCAGCAAGGGCTGGCAAAACTACGCCAGCCGTGGATCGATgcgc > 1:687174/1‑69 (MQ=255) gTGATCCGCAGATTGCCATTAACGTGCAGCAAGGGCTGGCAAAACTACGCCAGCCGTGGATCGATgcgc > 1:671020/1‑69 (MQ=255) gTGATCCGCAGATTGCCATTAACGTGCAGCAAGGGCTGGCAAAACTACGCCAGCCGTGGATCGATgcgc > 1:2043356/1‑69 (MQ=255) gTGATCCGCAGATTGCCATTAACGTGCAGCAACGGCTGGCAAAACTACGCCAGCCGTGGATCGATgcgc > 1:255138/1‑69 (MQ=255) ccGCAGATTGCCATTAACGTGCAGCAAGGGCTGGCAAAACTAc > 1:2637647/1‑43 (MQ=255) ccGCAGATTGCCATTAACGTGCAGCAAGGGCTGGCAAAACTAc > 1:1038308/1‑43 (MQ=255) ccGCAGATTGCCATTAACGTGCAGCAAGGGCTGGCAAAACTAc > 1:3151838/1‑43 (MQ=255) ccGCAGATTGCCATTAACGTGCAGCAAGGGCTGGCAAAACTAc > 1:1478604/1‑43 (MQ=255) ccGCAGATTGCCATTAACGTGCAGCAAGGGCTGGCAAAACTAc > 1:1619353/1‑43 (MQ=255) ccGCAGATTGCCATTAACGTGCAGCAAGGGCTGGCAAAACTAc > 1:1907748/1‑43 (MQ=255) ccGCAGATTGCCATTAACGTGCAGCAAGGGCTGGCAAAACTAc > 1:2058120/1‑43 (MQ=255) ccGCAGATTGCCATTAACGTGCAGCAAGGGCTGGCAAAACTAc > 1:2384555/1‑43 (MQ=255) ccGCAGATTGCCATTAACGTGCAGCAAGGGCTGGCAAAACTAc > 1:2567266/1‑43 (MQ=255) ccGCAGATTGCCATTAACGTGCAGCAAGGGCTGGCAAAACTAc > 1:264654/1‑43 (MQ=255) ccGCAGATTGCCATTAACGTGCAGCAAGGGCTGGCAAAACTACGCCAGCCGTGGATCGATGCGCgcggc < 1:631664/69‑1 (MQ=255) ccGCAGATTGCCATTAACGTGCAGCAAGGGCTGGCAAAACTACGCCAGCCGTGGATCGATGCGCgcggc > 1:59691/1‑69 (MQ=255) ccGCAGATTGCCATTAACGTGCAGCAAGGGCTGGCAAAACTACGCCAGCCGTGGATCGATGCGCgcggc < 1:51168/69‑1 (MQ=255) ccGCAGATTGCCATTAACGTGCAGCAAGGGCTGGCAAAACTACGCCAGCCGTGGATCGATGCGCgcggc < 1:3418263/69‑1 (MQ=255) ccGCAGATTGCCATTAACGTGCAGCAAGGGCTGGCAAAACTACGCCAGCCGTGGATCGATGCGCgcggc < 1:694235/69‑1 (MQ=255) ccGCAGATTGCCATTAACGTGCAGCAAGGGCTGGCAAAACTACGCCAGCCGTGGATCGATGCGCgcggc < 1:582385/69‑1 (MQ=255) ccGCAGATTGCCATTAACGTGCAGCAAGGGCTGGCAAAACTACGCCAGCCGTGGATCGATGCGCgcggc > 1:937645/1‑69 (MQ=255) ccGCAGATTGCCATTAACGTGCAGCAAGGGCTGGCAAAACTACGCCAGCCGTGGATCGATGCGCgcggc < 1:574381/69‑1 (MQ=255) ccGCAGATTGCCATTAACGTGCAGCAAGGGCTGGCAAAACTACGCCAGCCGTGGATCGATGCGCgcggc < 1:2688427/69‑1 (MQ=255) ccGCAGATTGCCATTAACGTGCAGCAAGGGCTGGCAAAACTACGCCAGCCGTGGATCGATGCGCgcggc > 1:337963/1‑69 (MQ=255) ccGCAGATTGCCATTAACGTGCAGCAAGGGCTGGCAAAACTACGCCAGCCGTGGATCGATGCGCgcggc > 1:2323508/1‑69 (MQ=255) ccGCAGATTGCCATTAACGTGCAGCAAGGGCTGGCAAAACTACGCCAGCCGTGGATCGATGCGCgcggc < 1:1491478/69‑1 (MQ=255) ccGCAGATTGCCATTAACGTGCAGCAAGGGCTGGCAAAACTACGCCAGCCGTGGATCGATGCGCgcggc > 1:1608338/1‑69 (MQ=255) ccGCAGATTGCCATTAACGTGCAGCAAGGGCTGGCAAAACTACGCCAGCCGTGGATCGATGCGCgcggc < 1:1645268/69‑1 (MQ=255) ccGCAGATTGCCATTAACGTGCAGCAAGGGCTGGCAAAACTACGCCAGCCGTGGATCGATGCGCgcggc > 1:1724805/1‑69 (MQ=255) ccGCAGATTGCCATTAACGTGCAGCAAGGGCTGGCAAAACTACGCCAGCCGTGGATCGATGCGCgcggc < 1:1753777/69‑1 (MQ=255) ccGCAGATTGCCATTAACGTGCAGCAAGGGCTGGCAAAACTACGCCAGCCGTGGATCGATGCGCgcggc < 1:1974448/69‑1 (MQ=255) ccGCAGATTGCCATTAACGTGCAGCAAGGGCTGGCAAAACTACGCCAGCCGTGGATCGATGCGCgcggc > 1:2093444/1‑69 (MQ=255) ccGCAGATTGCCATTAACGTGCAGCAAGGGCTGGCAAAACTACGCCAGCCGTGGATCGATGCGCgcggc > 1:3362761/1‑69 (MQ=255) ccGCAGATTGCCATTAACGTGCAGCAAGGGCTGGCAAAACTACGCCAGCCGTGGATCGATGCGCgcggc > 1:1488098/1‑69 (MQ=255) ccGCAGATTGCCATTAACGTGCAGCAAGGGCTGGCAAAACTACGCCAGCCGTGGATCGATGCGCgcggc < 1:2715934/69‑1 (MQ=255) ccGCAGATTGCCATTAACGTGCAGCAAGGGCTGGCAAAACTACGCCAGCCGTGGATCGATGCGCgcggc < 1:2758134/69‑1 (MQ=255) ccGCAGATTGCCATTAACGTGCAGCAAGGGCTGGCAAAACTACGCCAGCCGTGGATCGATGCGCgcggc > 1:2974527/1‑69 (MQ=255) ccGCAGATTGCCATTAACGTGCAGCAAGGGCTGGCAAAACTACGCCAGCCGTGGATCGATGCGCgcggc < 1:303544/69‑1 (MQ=255) cGCAGATTGCCATTAACGTGCAGCAAGGGCTGTCAAAACTACGCCAGCCGTGGATCGATGCGCgcggc < 1:599599/68‑1 (MQ=255) cGCAGATTGCCATTAACGTGCAGCAAGGGCTGGCAAAACTACGCCAGCCGTGGATCGATGCGCgcggcg < 1:2393621/69‑1 (MQ=255) cGCAGATTGCCATTAACGTGCAGCAAGGGCTGGCAAAACTACGCCAGCCGTGGATCGATGCGCgcggcg < 1:1266866/69‑1 (MQ=255) cGCAGATTGCCATTAACGTGCAGCAAGGGCTGGCAAAACTACGCCAGCCGTGGATCGATGCGCgcggcg < 1:525374/69‑1 (MQ=255) cGCAGATTGCCATTAACGTGCAGCAAGGGCTGGCAAAACTACGCCAGCCGTGGATCGATGCGCgcggcg < 1:1898773/69‑1 (MQ=255) cGCAGATTGCCATTAACGTGCAGCAAGGGCTGGCAAAACTACGCCAGCCGTGGATCGATGCGCgcggcg < 1:1087837/69‑1 (MQ=255) aTTGCCATTAACGTGCAGCAAGGGCTGGCAAAACTACGCCAGCCGTGGATCGATGCGCgcggc < 1:1435735/63‑1 (MQ=255) | GCAGTACGAAGAAAACGAAGCGATTCCGGTCTACGACACCTCCGGCCCGTATGGTGATCCGCAGATTGCCATTAACGTGCAGCAAGGGCTGGCAAAACTACGCCAGCCGTGGATCGATGCGCGCGGCG > minE/2246476‑2246603 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |