| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,247,184 | G→A | 78.7% | A299A (GCG→GCA) | thiC → | thiamin (pyrimidine moiety) biosynthesis protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,247,184 | 0 | G | A | 78.7% | 76.8 / 13.6 | 56 | A299A (GCG→GCA) | thiC | thiamin (pyrimidine moiety) biosynthesis protein |
| Reads supporting (aligned to +/- strand): ref base G (6/6); new base A (23/21); total (29/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01 | |||||||||||
CACGAAACCCGCGAGTGGATTTTGCGTAACAGCCCGGTGCCGATCGGTACAGTGCCGATCTACCAGGCGCTGGAGAAGGTTAACGGGATCGCCGAAGATCTTACCTGGGAAGCGTTCCGCGACACGCTGCTGG > minE/2247116‑2247248 | cACGAAACCCGCGAGTGGATTTTGCGTAACAGCCCGGTGCCGATCGGTACAGTGCCGATCTACCAGgcg > 1:1038318/1‑69 (MQ=255)cACGAAACCCGCGAGTGGATTTTGCGTAACAGCCCGGTGCCGATCGGTACAGTGCCGATCTACCAGgcg > 1:2833228/1‑69 (MQ=255)cACGAAACCCGCGAGTGGATTTTGCGTAACAGCCCGGTGCCGATCGGTACAGTGCCGATCTACCAGgcg > 1:2075464/1‑69 (MQ=255)cACGAAACCCGCGAGTGGATTTTGCGTAACAGCCCGGTGCCGATCGGTACAGTGCCGATCTACCAGgcg > 1:505848/1‑69 (MQ=255)cACGAAACCCGCGAGTGGATTTTGCGTAACAGCCCGGTGCCGATCGGTACAGTGCCGATCTACCAGgcg > 1:715165/1‑69 (MQ=255)cACGAAACCCGCGAGTGGATTTTGCGTAACAGCCCGGTGCCGATCGGTACAGTGCCGATCTACCAGgcg > 1:877486/1‑69 (MQ=255) ccGCGAGTGGATTTTGCGTAACAGCCCGGTGCCGATCGGTACAGTGCCGATCTACCAGGCGCTGGAGaa < 1:592883/69‑1 (MQ=255) gATTTTGCGTAACAGCCCGGTGCCGATCGGTACAGTGCCGATCTACCAGGCGCTGGAGAAGGTTAACgg < 1:1449836/69‑1 (MQ=255) gATTTTGCGTAACAGCCCGGTGCCGATCGGTACAGTGCCGATCTACCAGGCGCTGGAGAAGGTTAACgg < 1:2026559/69‑1 (MQ=255) ccGGTGCCGATCGGTACAGTGCCGATCTACAAGGCGCTGGAGAAGGTTAACGGGATCGCCGAAGATCtt < 1:919551/69‑1 (MQ=255) gCCGATCGGTACAGTGCCGATCTACCAGGCGCTGGAGAAGGTTAACGGGATCGCCGaa > 1:3325659/1‑58 (MQ=255) gATCGGTACAGTGCCGATCTACCAGGCACTTGAGAAGGTGAACGGGATCGCTGAAGATCTTACCTggg < 1:429437/68‑1 (MQ=255) gATCGGTACAGTGCCGATCTACCAGGCACTGGAGAATGTGAACGGGATCGCTGAAGATCTTACCTggg < 1:3398121/68‑1 (MQ=255) gATCGGTACAGTGCCGATCTACCAGGCACTGGAGAATGTGAACGGGATCGCTGAAGATCTTACCTggg < 1:3394317/68‑1 (MQ=255) gATCGGTACAGTGCCGATCTACCAGGCACTGGAGAAGGTGAACGGGATCGCTGAAGATCTTACCTggg < 1:3392697/68‑1 (MQ=255) gATCGGTACAGTGCCGATCTACCAGGCACTGGAGAAGGTGAACGGGATCGCTGAAGATCTTACCTggg < 1:1054879/68‑1 (MQ=255) gATCGGTACAGTGCCGATCTACCAGGCACTGGAGAAGGTGAACGGGATCGCTGAAGATCTTACCTggg < 1:2850322/68‑1 (MQ=255) gATCGGTACAGTGCCGATCTACCAGGCACTGGAGAAGGTGAACGGGATCGCTGAAGATCTTACCTggg < 1:3029456/68‑1 (MQ=255) gATCGGTACAGTGCCGATCTACCAGGCACTGGAGAAGGTGAACGGGATCGCTGAAGATCTTACCTggg < 1:3059565/68‑1 (MQ=255) gATCGGTACAGTGCCGATCTACCAGGCACTGGAGAAGGTGAACGGGATCGCTGAAGATCTTACCTggg < 1:3134693/68‑1 (MQ=255) gATCGGTACAGTGCCGATCTACCAGGCACTGGAGAAGGTGAACGGGATCGCTGAAGATCTTACCTggg < 1:3149009/68‑1 (MQ=255) gATCGGTACAGTGCCGATCTACCAGGCACTGGAGAAGGTGAACGGGATCGCTGAAGATCTTACCTggg < 1:854296/68‑1 (MQ=255) gATCGGTACAGTGCCGATCTACCAGGCACTGGAGAAGGTGAACGGGATCGCTGAAGATCTTACCTggg < 1:344877/68‑1 (MQ=255) gATCGGTACAGTGCCGATCTACCAGGCACTGGAGAAGGTGAACGGGATCGCTGAAGATCTTACCTggg < 1:462792/68‑1 (MQ=255) gATCGGTACAGTGCCGATCTACCAGGCACTGGAGAAGGTGAACGGGATCGCTGAAGATCTTACCTggg < 1:636367/68‑1 (MQ=255) gATCGGTACAGTGCCGATCTACCAGGCACTGGAGAAGGTGAACGGGATCGCTGAAGATCTTACCTggg < 1:2634740/68‑1 (MQ=255) gATCGGTACAGTGCCGATCTACCAGGCACTGGAGAAGGTGAACGGGATCGCTGAAGATCTTACCTggg < 1:1808094/68‑1 (MQ=255) gATCGGTACAGTGCCGATCTACCAGGCACTGGAGAAGGTGAACGGGATCGCTGAAGATCTTACCTggg < 1:1808382/68‑1 (MQ=255) gATCGGTACAGTGCCGATCTACCAGGCACTGGAGAAGGTGAACGGGATCGCTGAAGATCTTACCTggg < 1:1558868/68‑1 (MQ=255) gATCGGTACAGTGCCGATCTACCAGGCACTGGAGAAGGTGAACGGGATCGCTGAAGATCTTACCTggg < 1:224455/68‑1 (MQ=255) gATCGGTACAGTGCCGATCTACCAGGCACTGGAGAAGGTGAACGGGATCGCTGAAGATCTTACCTggg < 1:2293447/68‑1 (MQ=255) cGGTACAGTGCCGATCTACCAGGCGCTGGAGAAGGTTAACGGGATCGCCGAAGATCTTACCTGGGAAGc > 1:80103/1‑69 (MQ=255) cGGTACAGTGCCGATCTACCAGGCGCTGGAGAAGGTTAACGGGATCGCCGAAGATCTTACCTGGGAAGc > 1:2986796/1‑69 (MQ=255) gTGCCGATCTACCAGGCGCTGGAGAAGGTTAACGGGATCGCCGAAGATCTTACCTGGGAAGCGTTCcgc > 1:1277055/1‑69 (MQ=255) gTGCCGATCTACAAGGCGCTGGAGAAGGTTAACGGGATCGCCGAAg > 1:609717/1‑46 (MQ=255) gTGCCGATCTAACAGGCGCTGGAGAAGGTTAACGGGATCGCCGAAGATCTTACCTGGGAAGCGTTCcgc > 1:3306537/1‑69 (MQ=255) cGATCTACCAGGCGCTGGAGAAGGTTAACGGGATCGCCGAAGATCtt < 1:1271413/47‑1 (MQ=255) cTACCAGGCACTGGAGAAGGTGAACGGGATCGCTGAAGATCTTACCTggg < 1:1457261/50‑1 (MQ=255) aGGCGCTGGAGAAGGTTAACGGGATCGCCGAAGATCTTACCTGGGAAGCGTTCCGCGACACGCTGCTgg < 1:1854687/69‑1 (MQ=255) aGGCACTGGAGAAGGTGAACGGGATCGCTGAAGATCTTACCTGGGAAGTCTTcc > 1:3276893/1‑54 (MQ=255) aGGCACTGGAGAAGGTGAACGGGATCGCTGAAGATCTTACCTGGGAAGTCTTTCGCGACACCCTGCTgg > 1:1388572/1‑69 (MQ=255) aGGCACTGGAGAAGGTGAACGGGATCGCTGAAGATCTTACCTGGGAAGTCTTCCGCGACACcctgctcg > 1:1174307/1‑67 (MQ=255) aGGCACTGGAGAAGGTGAACGGGATCGCTGAAGATCTTACCTGGGAAGTCTTCCGCGACACCCTGCTgg > 1:137709/1‑69 (MQ=255) aGGCACTGGAGAAGGTGAACGGGATCGCTGAAGATCTTACCTGGGAAGTCTTCCGCGACACCCTGCTgg > 1:568201/1‑69 (MQ=255) aGGCACTGGAGAAGGTGAACGGGATCGCTGAAGATCTTACCTGGGAAGTCTTCCGCGACACCCTGCTgg > 1:1465871/1‑69 (MQ=255) aGGCACTGGAGAAGGTGAACGGGATCGCTGAAGATCTTACCTGGGAAGTCTTCCGCGACACCCTGCTgg > 1:437203/1‑69 (MQ=255) aGGCACTGGAGAAGGTGAACGGGATCGCTGAAGATCTTACCTGGGAAGTCTTCCGCGACACCCTGCTgg > 1:2414550/1‑69 (MQ=255) aGGCACTGGAGAAGGTGAACGGGATCGCTGAAGATCTTACCTGGGAAGTCTTCCGCGACACCCTGCTgg > 1:1781720/1‑69 (MQ=255) aGGCACTGGAGAAGGTGAACGGGATCGCTGAAGATCTTACCTGGGAAGTCTTCCGCGACACCCTGCTgg > 1:1827147/1‑69 (MQ=255) aGGCACTGGAGAAGGTGAACGGGATCGCTGAAGATCTTACCTGGGAAGTCTTCCGCGACACCCTGCTgg > 1:3385221/1‑69 (MQ=255) aGGCACTGGAGAAGGTGAACGGGATCGCTGAAGATCTTACCTGGGAAGTCTTCCGCGACACCCTGCTgg > 1:1899298/1‑69 (MQ=255) aGGCACTGGAGAAGGTGAACGGGATCGCTGAAGATCTTACCTGGGAAGTCTTCCGCGACACCCTGCTgg > 1:3245168/1‑69 (MQ=255) aGGCACTGGAGAAGGTGAACGGGATCGCTGAAGATCTTACCTGGGAAGTCTTCCGCGACACCCTGCTgg > 1:3196795/1‑69 (MQ=255) aGGCACTGGAGAAGGTGAACGGGATCGCTGAAGATCTTACCTGGGAAGTCTTCCGCGACACCCTGCTgg > 1:3173541/1‑69 (MQ=255) aGGCACTGGAGAAGGTGAACGGGATCGCTGAAGATCTTACCTGGGAAGTCTTCCGCGACACCCTGCTgg > 1:1990573/1‑69 (MQ=255) aGGCACTGGAGAAGGTGAACGGGATCGCTGAAGATCTTACCTGGGAAGTCTTCCGCGACACCCTGCTgg > 1:2221891/1‑69 (MQ=255) aGGCACTGGAGAAGGTGAACGGGATCGCTGAAGATCTTACCTGGGAAGTCTTCCGCGACACCCTGCTgg > 1:3045386/1‑69 (MQ=255) aGGCACTGGAGAAGGTGAACGGGATCGCTGAAGATCTTACCTGGGAAGTCTTCCGCGACACCCTGCTgg > 1:3012144/1‑69 (MQ=255) aGGCACTGGAGAAGGTGAACGGGATCGCTGAAGATCTTACCTGGGAAGTCTTCCGCGACACCCTGCTgg > 1:2897907/1‑69 (MQ=255) aGGCACTGGAGAAGGTGAACGGGATCGCTGAAGATCTTACCTGGGAAGTCTTCCGCGACACCCTGCTgg > 1:2872015/1‑69 (MQ=255) aGGCACTGGAGAAGGTGAACGGGATCGCTGAAGATATTACCTGGGAAGTCTTCCGCGACACCctgctg > 1:970993/1‑68 (MQ=255) aGGCACAGGAGAAGGTGAACGGGATCGCTGAAGATCTTACCTGGGAAGTCTTCCGCGACACCCTGCTgg > 1:435419/1‑69 (MQ=255) | CACGAAACCCGCGAGTGGATTTTGCGTAACAGCCCGGTGCCGATCGGTACAGTGCCGATCTACCAGGCGCTGGAGAAGGTTAACGGGATCGCCGAAGATCTTACCTGGGAAGCGTTCCGCGACACGCTGCTGG > minE/2247116‑2247248 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |