| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,247,796 | T→C | 51.0% | G503G (GGT→GGC) | thiC → | thiamin (pyrimidine moiety) biosynthesis protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,247,796 | 0 | T | C | 51.0% | 5.1 / 92.5 | 106 | G503G (GGT→GGC) | thiC | thiamin (pyrimidine moiety) biosynthesis protein |
| Reads supporting (aligned to +/- strand): ref base T (27/25); new base C (31/23); total (58/48) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.97e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 112 total aligned reads displayed.
GGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAGGGGCTTATCACCTATAAGATTGCTGCCCACGCCGCTGA > minE/2247728‑2247861 | ggTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGCATCTGGGt > 1:2283559/1‑69 (MQ=255)ggTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGCATCTGGGt > 1:263650/1‑69 (MQ=255)ggTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGCATCTGGGt > 1:3188762/1‑69 (MQ=255)ggTGCGGCGATGATTGGCTGCTTTGGCTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGCATCTGGGt > 1:1261034/1‑69 (MQ=255) gTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGCATCTGGGTc > 1:600658/1‑69 (MQ=255) gTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGCATCTGGGTc > 1:872281/1‑69 (MQ=255) gTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGCATCTGGGTc > 1:2752563/1‑69 (MQ=255) gTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGCATCTGGGTc > 1:2952108/1‑69 (MQ=255) gTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGCATCTGGGTc > 1:687796/1‑69 (MQ=255) ggcgATGATTGGCTGGTTTGGTTGTGCAATGCTCTGCTACGTAACGCCGAAGGAGCATCTGGGCCTGc > 1:3009780/1‑68 (MQ=255) ggcgATGATTGGCTGGTTTGGTTGTGCAATGCTCTGCTACGTAACGCCGAAAGAGCATCTGGGCCTGc > 1:816384/1‑68 (MQ=255) ggcgATGATTGGCTGGTTTGGTTGTGCAATGCTCTGCTACGTAACGCCGAAAGAGCATCTGGGCCTGc > 1:708739/1‑68 (MQ=255) ggcgATGATTGGCTGGTTTGGTTGTGCAATGCTCTGCTACGTAACGCCGAAAGAGCATCTGGGCCTGc > 1:2849470/1‑68 (MQ=255) ggcgATGATTGGCTGGTTTGGTTGTGCAATGCTCTGCTACGTAACGCCGAAAGAGCATCTGGGCCTGc > 1:3320165/1‑68 (MQ=255) ggcgATGATTGGCTGGTTTGGTTGTGCAATGCTCTGCTACGTAACGCCGAAAGAGCATCTGGGCCTGc > 1:3434621/1‑68 (MQ=255) ggcgATGATTGGCTGGTTTGGTTGTGCAATGCTCTGCTACGTAACGCCGAAAGAGCATCTGGGCCTGc > 1:261194/1‑68 (MQ=255) ggcgATGATTGGCTGGTTTGGTTGTGCAATGCTCTGCTACGTAACGCCGAAAGAGCATCTGGGCCTGc > 1:102224/1‑68 (MQ=255) ggcgATGATTGGCTGGTTTGGTTGTGCAATGCTCTGCTACGTAACGCCGAAAGAGCATCTGGGCCTGc > 1:2216756/1‑68 (MQ=255) ggcgATGATTGGCTGGTTTGGTTGTGCAATGCTCTGCTACGTAACGCCGAAAGAGCATCTGGGCCTGc > 1:2114421/1‑68 (MQ=255) ggcgATGATTGGCTGGTTTGGTTGTGCAATGCTCTGCTACGTAACGCCGAAAGAGCATCTGGGCCTGc > 1:2015281/1‑68 (MQ=255) ggcgATGATTGGCTGGTTTGGTTGTGCAATGCTCTGCTACGTAACGCCGAAAGAGCATCTGGGCCTGc > 1:875263/1‑68 (MQ=255) ggcgATGATTGGCTGGTTTGGTTGTGCAATGCTCTGCTACGTAACGCCGAAAGAGCATCTGGGCCTGc > 1:1767631/1‑68 (MQ=255) gcgATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGCATCTGGGTCTGccc > 1:2228674/1‑69 (MQ=255) gcgATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGCATCTGGGTCTGccc > 1:2644679/1‑69 (MQ=255) gcgATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGCATCTGGGTCTGccc > 1:2776149/1‑69 (MQ=255) gcgATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGCATCTGGGTCTGccc > 1:2151587/1‑69 (MQ=255) gcgATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGCATCTGGGTCTGccc > 1:148201/1‑69 (MQ=255) gcgATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGCATCTGGGTCTGcc > 1:314709/1‑68 (MQ=255) cgATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGCATCTGGGTCTGCCCa < 1:832852/69‑1 (MQ=255) cgATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGCATCTGGGTCTGCCCa < 1:592876/69‑1 (MQ=255) gatgatTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGCATCTGGGTCTGCCCa < 1:183725/68‑1 (MQ=255) tgatTGGCTGGTTTGGTTGTGCAATGCTCTGCTACGTAGCGCCGAAAGAGCATCTGGGCCTGCCaaa > 1:87443/1‑67 (MQ=255) tgatTGGCTGGTTTGGTTGTGCAATGCTCTGCTACGTAACGCCGAAAGAGCATCTGGGCCTGCCaaa > 1:2794868/1‑67 (MQ=255) tgatTGGCTGGTTTGGTTGTGCAATGCTCTGCTACGTAACGCCGAAAGAGCATCTGGGCCTGCCaaa > 1:817453/1‑67 (MQ=255) tgatTGGCTGGTTTGGTTGTGCAATGCTCTGCTACGTAACGCCGAAAGAGCATCTGGGCCTGCCaaa > 1:848249/1‑67 (MQ=255) tgatTGGCTGGTTTGGTTGTGCAATGCTCTGCTACGTAACGCCGAAAGAGCATCTGGGCCTGCCaaa > 1:2930824/1‑67 (MQ=255) tgatTGGCTGGTTTGGTTGTGCAATGCTCTGCTACGTAACGCCGAAAGAGCATCTGGGCCTGCCaaa > 1:864697/1‑67 (MQ=255) tgatTGGCTGGTTTGGTTGTGCAATGCTCTGCTACGTAACGCCGAAAGAGCATCTGGGCCTGCCaaa > 1:275909/1‑67 (MQ=255) tgatTGGCTGGTTTGGTTGTGCAATGCTCTGCTACGTAACGCCGAAAGAGCATCTGGGCCTGCCaaa > 1:2709129/1‑67 (MQ=255) tgatTGGCTGGTTTGGTTGTGCAATGCTCTGCTACGTAACGCCGAAAGAGCATCTGGGCCTGCCaaa > 1:889290/1‑67 (MQ=255) tgatTGGCTGGTTTGGTTGTGCAATGCTCTGCTACGTAACGCCGAAAGAGCATCTGGGCCTGCCaaa > 1:3183121/1‑67 (MQ=255) tgatTGGCTGGTTTGGTTGTGCAATGCTCTGCTACGTAACGCCGAAAGAGCATCTGGGCCTGCCaaa > 1:2585097/1‑67 (MQ=255) tgatTGGCTGGTTTGGTTGTGCAATGCTCTGCTACGTAACGCCGAAAGAGCATCTGGGCCTGCCaaa > 1:1252773/1‑67 (MQ=255) tgatTGGCTGGTTTGGTTGTGCAATGCTCTGCTACGTAACGCCGAAAGAGCATCTGGGCCTGCCaaa > 1:2218583/1‑67 (MQ=255) tgatTGGCTGGTTTGGTTGTGCAATGCTCTGCTACGTAACGCCGAAAGAGCATCTGGGCCTGCCaaa > 1:2102769/1‑67 (MQ=255) tgatTGGCTGGTTTGGTTGTGCAATGCTCTGCTACGTAACGCCGAAAGAGCATCTGGGCCTGCCaaa > 1:2074205/1‑67 (MQ=255) ggCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGCATCTGGGTCTGCCCAATAaagaa < 1:3137992/69‑1 (MQ=255) ggCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGCATCTGGGTCTGCCCAATAaagaa < 1:1751914/69‑1 (MQ=255) ggCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGCATCTGGGTCTGCCCAATAaagaa < 1:874174/69‑1 (MQ=255) tGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCAATAGAGCATCTGGGTCTGCCCAATAAAGAAGAt < 1:2570713/69‑1 (MQ=255) tGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGAt < 1:298364/69‑1 (MQ=255) tGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGAt < 1:1185692/69‑1 (MQ=255) tGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGAt < 1:455748/69‑1 (MQ=255) tGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGAt < 1:542468/69‑1 (MQ=255) tGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGAt < 1:848231/69‑1 (MQ=255) tGGCTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTa > 1:845570/1‑68 (MQ=255) tGGCTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTa > 1:1002472/1‑68 (MQ=255) tGGCTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTa > 1:1233943/1‑68 (MQ=255) tGGCTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTa > 1:2274385/1‑68 (MQ=255) tGGCTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTa > 1:1438205/1‑68 (MQ=255) ctctGTTACGTAACGCCAAAAGAGCATCTGGGTCTGCCCAATAaagaa < 1:1602954/48‑1 (MQ=255) ctctGTTACGTAACGCCAAAAGAGCATCTGGGTCTGCCCAATAaagaa < 1:786592/48‑1 (MQ=255) ctctGTTACGTAACGCCAAAAGAGCATCTGGGTCTGCCCAATAaagaa < 1:670474/48‑1 (MQ=255) ctctGTTACGTAACGCCAAAAGAGCATCTGGGTCTGCCCAATAaagaa < 1:3377719/48‑1 (MQ=255) ctctGTTACGTAACGCCAAAAGAGCATCTGGGTCTGCCCAATAaagaa < 1:3424629/48‑1 (MQ=255) ctGTTACGTAACGCCAAAAGAGGATCTGGGTCTGCCCAATAaagaa < 1:1177201/46‑1 (MQ=255) ctGTTACGTAACGCCAAAAGAGCATCTGGGTCTGCCCAATAaagaa < 1:2616169/46‑1 (MQ=255) tcGCCGAAAGAGCATCTGGGCCTGCCAAATAAAGAAGATGTTAAACAGGGGCTTATCACCTATAAGAt < 1:2651775/67‑1 (MQ=255) aCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAGGGGCTTATCACCTATAAGAtt > 1:2027847/1‑69 (MQ=255) aCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAGGGGCTTATCACCTATAAGAtt > 1:2075410/1‑69 (MQ=255) aCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAGGGGCTTATCACCTATAAGAtt > 1:1923813/1‑69 (MQ=255) aCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAGGGGCTTATCACCTATAAGAtt > 1:3424539/1‑69 (MQ=255) aCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAGGGGCTTATCACCTATAAGAtt > 1:189717/1‑69 (MQ=255) aCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAGGGGCTTATCACCTATAAGAtt > 1:1731092/1‑69 (MQ=255) cgaaaGAGCATCTGGGCCTGCCAAATAAAGAAGATGTTAAACAGGGGCTTATCACCTATAAGAt < 1:98066/62‑1 (MQ=255) cgaaaGAGCATCTGGGCCTGCCAAATAAAGAAGATGTTAAACAGGGGCTTATCACCTATAAGAt < 1:898183/62‑1 (MQ=255) cgaaaGAGCATCTGGGCCTGCCAAATAAAGAAGATGTTAAACAGGGGCTTATCACCTATAAGAt < 1:821246/62‑1 (MQ=255) cgaaaGAGCATCTGGGCCTGCCAAATAAAGAAGATGTTAAACAGGGGCTTATCACCTATAAGAt < 1:401369/62‑1 (MQ=255) cgaaaGAGCATCTGGGCCTGCCAAATAAAGAAGATGTTAAACAGGGGCTTATCACCTATAAGAt < 1:1055898/62‑1 (MQ=255) cgaaaGAGCATCTGGGCCTGCCAAATAAAGAAGATGTTAAACAGGGGCTTATCACCTATAAGAt < 1:1187109/62‑1 (MQ=255) cgaaaGAGCATCTGGGCCTGCCAAATAAAGAAGATGTTAAACAGGGGCTTATCACCTATAAGAt < 1:1736096/62‑1 (MQ=255) cgaaaGAGCATCTGGGCCTGCCAAATAAAGAAGATGTTAAACAGGGGCTTATCACCTATAAGAt < 1:2392021/62‑1 (MQ=255) cgaaaGAGCATCTGGGCCTGCCAAATAAAGAAGATGTTAAACAGGGGCTTATCACCTATAAGAt < 1:2647695/62‑1 (MQ=255) cgaaaGAGCATCTGGGCCTGCCAAATAAAGAAGATGTTAAACAGGGGCTTATCACCTATAAGAt < 1:2783251/62‑1 (MQ=255) cgaaaGAGCATCTGGGCCTGCCAAATAAAGAAGATGTTAAACAGGGGCTTATCACCTATAAGAt < 1:27962/62‑1 (MQ=255) cgaaaGAGCATCTGGGCCTGCCAAATAAAGAAGATGTTAAACAGGGGCTTATCACCTATAAGAt < 1:2845445/62‑1 (MQ=255) cgaaaGAGCATCTGGGCCTGCCAAATAAAGAAGATGTTAAACAGGGGCTTATCACCTATAAGAt < 1:2860474/62‑1 (MQ=255) cgaaaGAGCATCTGGGCCTGCCAAATAAAGAAGATGTTAAACAGGGGCTTATCACCTATAAGAt < 1:2917107/62‑1 (MQ=255) cgaaaGAGCATCTGGGCCTGCCAAATAAAGAAGATGTTAAACAGGGGCTTATCACCTATAAGAt < 1:319057/62‑1 (MQ=255) cgaaaGAGCATCTGGGCCTGCCAAATAAAGAAGATGTTAAACAGGGGCTTATCACCTATAAGAt < 1:3295808/62‑1 (MQ=255) cgaaaGAGCATCTGGGCCTGCCAAATAAAGAAGATGTTAAACAGGGGCTTATCACCTATAAGAt < 1:329633/62‑1 (MQ=255) cgaaaGAGCATCTGGGCCTGCCAAATAAAGAAGATGTTAAACAGGGGCTTATCACCTATAAGAt < 1:538773/62‑1 (MQ=255) aaGAGCATCTGGGCCTGCCAAATAAAGAAGATGTTAAACAGGGGCTTATCACCTATAAGAt < 1:2779373/61‑1 (MQ=255) gagCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAGGGGCTTATCACCTATAAGATTGCTGCCCAc < 1:2846067/69‑1 (MQ=255) gagCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAGGGGCTTATCACCTATAAGATTGCTGCCCAc < 1:696927/69‑1 (MQ=255) gagCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAGGGGCTTATCACCTATAAGATTGCTGCCCAc < 1:211815/69‑1 (MQ=255) gagCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAGGGGCTTATCACCTATAAGATTGCTGCCCAc < 1:159443/69‑1 (MQ=255) gagCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAGGGGCTTATCACCTATAAGATTGCTGCCCAc < 1:635565/69‑1 (MQ=255) cTGGGCCTGCCAAATAAAGAAGATGTTAAACAGGGGCTTATCACCTATAAGAt < 1:3189139/53‑1 (MQ=255) tCTGCCCAATAAAGAAGATGTTAAGCAGGGGCTTATCACCTATAAGATTGCTGCCCACGCCGCTGa < 1:3359972/66‑1 (MQ=255) | GGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAGGGGCTTATCACCTATAAGATTGCTGCCCACGCCGCTGA > minE/2247728‑2247861 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |