| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,248,786 | T→C | 27.7% | L202L (TTG→CTG) | thiE → | thiamin phosphate synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,248,786 | 0 | T | C | 27.7% | 93.5 / 37.2 | 90 | L202L (TTG→CTG) | thiE | thiamin phosphate synthase |
| Reads supporting (aligned to +/- strand): ref base T (24/41); new base C (4/21); total (28/62) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.52e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGCAGTATCGCCGTCGTCAGCGCCATTACTCAAGCCGCAGACTGGCGTTTGGCAACGGCACAGTTGCTGGAAATTGCAGGAGTTGGCGATGAATGACCGTGACTTTATGCGTTATAGCCGCCAAA > minE/2248723‑2248847 | ggCAGTATCGCCGTCGTCAGCGCCATTACTCAAGCCGCAGACTGGCGTTTGGCAACGGCACAGTTGc > 1:1877577/1‑67 (MQ=255)ggCAGTATCGCCGTCGTCAGCGCCATTACTCAAGCCGCAGACTGGCGTTTGGCAACGGCACAGTTGc > 1:3392109/1‑67 (MQ=255)ggCAGTATCGCCGTCGTCAGCGCCATTACTCAAGCCGCAGACTGGCGTTTGGCAACGGCACAGTTGCt > 1:400941/1‑68 (MQ=255)ggCAGTATCGCCGTCGTCAGCGCCATTACTCAAGCCGAAGACTGGCGTTTGGCAACGGCACAGTTGCt > 1:2315306/1‑68 (MQ=255) aGTATCGCCGTCGTCAGCGCCATTACTCAAGCCGCAGACTGGCGTTTGGCAACGGCACAGTTGCTGGaa < 1:2140708/69‑1 (MQ=255) aGTATCGCCGTCGTCAGCGCCATTACTCAAGCCGCAGACTGGCGTTTGGCAACGGCACAGTTGCTGGaa < 1:3062059/69‑1 (MQ=255) aGTATCGCCGTCGTCAGCGCCATTACTCAAGCCGCAGACTGGCGTTTGGCAACGGCACAGTTGCTGGaa < 1:2015386/69‑1 (MQ=255) aGTATCGCCGTCGTCAGCGCCATTACTCAAGCCGCAGACTGGCGTTTGGCAACGGCACAGTTGCTGGaa < 1:1675036/69‑1 (MQ=255) aGTATCGCCGTCGTCAGCGCCATTACTCAAGCCGCAGACTGGCGTTTGGCAACGGCACAGTTGCTGGaa < 1:361792/69‑1 (MQ=255) gTATCGCCGTCGTCAGCGCCATTACTCAAGCCGCAGACTGGCGTTTGGCAACGGCACAGTTGCTGGaaa > 1:1873523/1‑69 (MQ=255) gTATCGCCGTCGTCAGCGCCATTACTCAAGCCGCAGACTGGCGTTTGGCAACGGCACAGTTGCTGGaaa > 1:3393661/1‑69 (MQ=255) gTATCGCCGTCGTCAGCGCCATTACTCAAGCCGCAGACTGGCGTTTGGCAACGGCACAGTTGCTGGaaa > 1:1740586/1‑69 (MQ=255) gTATCGCCGTCGTCAGCGCCATTACTCAAGCCGCAGACTGGCGTTTGGCAACGGCACAGTTGCTGGaaa > 1:1736800/1‑69 (MQ=255) gTATCGCCGTCGTCAGCGCCATTACTCAAGCCGCAGACTGGCGTTTGGCAACGGCACAGTTGCTGGaaa > 1:1980943/1‑69 (MQ=255) gTATCGCCGTCGTCAGCGCCATTACTCAAGCCGCAGACTGGCGTTTGGCAACGGCACAGTTGCTGGaaa > 1:1555453/1‑69 (MQ=255) gTATCGCCGTCGTCAGCGCCATTACTCAAGCCGCAGACTGGCGTTTGGCAACGGCACAGTTGCTGGaaa > 1:1493276/1‑69 (MQ=255) gTATCGCCGTCGTCAGCGCCATTACTCAAGCCGCAGACTGGCGTTTGGCAACGGCACAGTTGCTGGaaa > 1:2142856/1‑69 (MQ=255) gTATCGCCGTCGTCAGCGCCATTACTCAAGCCGCAGACTGGCGTTTGGCAACGGCACAGTTGCTGGaaa > 1:2981000/1‑69 (MQ=255) gTATCGCCGTCGTCAGCGCCATTACTCAAGCCGCAGACTGGCGTTTGGCAACGGCACAGTTGCTGGaaa > 1:2466547/1‑69 (MQ=255) tCGCCGTCGTCAGCGCCATTACTCAAGCCGCAGACTGGCGTTTGGCAACGGCACAGTTGTTGGa < 1:256141/64‑1 (MQ=255) tCGCCGTCGTCAGCGCCATTACTCAAGCCGCAGACTGGCGTTTGGCAACGGCACAGTTGTTGGa < 1:3416988/64‑1 (MQ=255) tCGCCGTCGTCAGCGCCATTACTCAAGCCGCAGACTGGCGTTTGGCAACGGCACAGTTGTTGGa < 1:570107/64‑1 (MQ=255) tCGCCGTCGTCAGCGCCATTACTCAAGCCGCAGACTGGCGTTTGGCAACGGCACAGTTGTTGGa < 1:35168/64‑1 (MQ=255) tCGCCGTCGTCAGCGCCATTACTCAAGCCGCAGACTGGCGTTTGGCAACGGCACAGTTGTTGGa < 1:477531/64‑1 (MQ=255) tCGCCGTCGTCAGCGCCATTACTCAAGCCGCAGACTGGCGTTTGGCAACGGCACAGTTGCTGGa > 1:2782446/1‑64 (MQ=255) tCGCCGTCGTCAGCGCCATTACTCAAGCCGCAGACTGGCGTTTGGCAACGGCACAGTTGCTGGa > 1:2350697/1‑64 (MQ=255) tCGCCGTCGTCAGCGCCATTACTCAAGCCGCAGACTGGCGTTTGGCAACGGCACAGTTGCTGGAAAt > 1:1827543/1‑67 (MQ=255) gCCGTCGTCAGCGCCATTACTCAAGCCGCAGACTGGCGTTTGGCAACGGCACAGTTGCTGGAAATTGc > 1:1379529/1‑68 (MQ=255) gCCGTCGTCAGCGCCATTACTCAAGCCGCAGACTGGCGTTTGGCAACGGCACAGTTGCTGGAAATTGc > 1:920288/1‑68 (MQ=255) gCCGTCGTCAGCGCCATTACTCAAGCCGCAGACTGGCGTTTGGCAACGGCACAGTTGCTGGAAATTGc > 1:3115016/1‑68 (MQ=255) gCCGTCGTCAGCGCCATTACTCAAGCCGCAGACTGGCGTTTGGCAACGGCACAGTTGCTGGAAATTGc > 1:1548273/1‑68 (MQ=255) gCCGTCGTCAGCGCCATTACTCAAGCCGCAGACTGGCGTTTGGCAACGGCACAGTTGCTGGAAATTGc > 1:346355/1‑68 (MQ=255) gCCGTCGTCAGCGCCATTACTCAAGCCGCAGACTGGCGTTTGGCAACGGCACAGTTGCTGGAAATTGc > 1:1751051/1‑68 (MQ=255) gCCGTCGTCAGCGCCATTACTCAAGCCGCAGACTGGCGTTTGGCAACGGCACAGTTGCTGGAAATTGc > 1:1876086/1‑68 (MQ=255) cgtcgtCAGCGCCATTACTCAAGCCGCAGACTGGCGTTTGGCAACGGCACAGTTGCTGGAAATTGCAgg < 1:3067175/69‑1 (MQ=255) cgtcgtCAGCGCCATTACTCAAGCCGCAGACTGGCGTTTGGCAACGGCACAGTTGCTGGAAATTGCAgg < 1:38513/69‑1 (MQ=255) cgtcgtCAGCGCCATTACTCAAGCCGCAGACTGGCGTTTGGCAACGGCACAGTTGCTGGAAATTGCAgg < 1:2842193/69‑1 (MQ=255) cgtcgtCAGCGCCATTACTCAAGCCGCAGACTGGCGTTTGGCAACGGCACAGTTGCTGGAAATTGCAgg < 1:1234047/69‑1 (MQ=255) cgtcgtCAGCGCCATTACTCAAGCCGCAGACTGGCGTTTGGCAACGGCACAGTTGCTGGAAATTGCAgg < 1:1840333/69‑1 (MQ=255) cgtcgtCAGCGCCATTACTCAAGCCGCAGACTGGCGTTTGGCAACGGCACAGTTGCTGGAAATTGCAg < 1:2150651/68‑1 (MQ=255) cgtcgtCAGCGCCATTACTCAAGCCGCAGACTGGCGTTTGGCAACGGCACAGTTGCTGGAAATTGCAg < 1:1203556/68‑1 (MQ=255) gtcgtcAGCGCCATTACTCAAGCCGCAGACTGGCGTTTGGCAACGGCACAGTTGCTGGAAATTGCAgg < 1:2478233/68‑1 (MQ=255) gcgcCATTACTCAAGCCGCAGACTGGCGTTTGGCAACGGCACAgctgct > 1:2640630/1‑49 (MQ=255) gcgcCATTACTCAAGCCGCAGACTGGCGTTTGGCAACGGCACAGCTGCTGGAAATTGCAGGAGTTGGc > 1:2685866/1‑68 (MQ=255) gcgcCATTACTCAAGCCGCAGACTGGCGTTTGGCAACGGCACAGCTGCTGGAAATTGCAGGAGTTGGCg > 1:659502/1‑69 (MQ=255) gcgcCATTACTCAAGCCGCAGACTGGCGTTTGGCAACGGCACAGCTGCTGGAAATTGCAGGAGTTGGCg > 1:299108/1‑69 (MQ=255) gcgcCATTACTCAAGCCGCAGACTGGCGTTTGGCAACGGCACAGCTGCTGGAAATTGCAGGAGTTGGCg > 1:2294825/1‑69 (MQ=255) ccATTACTCAAGCCGCAGACTTGCGTTTGGCAACGGCACAGCTGCTGGAAATTGCAGGAGTTGGCGatg < 1:2979391/69‑1 (MQ=255) ccATTACTCAAGCCGCAGACTGGCGTTTGGCAACGGCACAGCTGCTGGAAATTGCAGGAGTTGGCGatg < 1:2128101/69‑1 (MQ=255) ccATTACTCAAGCCGCAGACTGGCGTTTGGCAACGGCACAGCTGCTGGAAATTGCAGGAGTTGGCGatg < 1:2601377/69‑1 (MQ=255) ccATTACTCAAGCCGCAGACTGGCGTTTGGCAACGGCACAGCTGCTGGAAATTGCAGGAGTTGGCGatg < 1:2021909/69‑1 (MQ=255) ccATTACTCAAGCCGCAGACTGGCGTTTGGCAACGGCACAGCTGCTGGAAATTGCAGGAGTTGGCGatg < 1:3083963/69‑1 (MQ=255) ccATTACTCAAGCCGCAGACTGGCGTTTGGCAACGGCACAGCTGCTGGAAATTGCAGGAGTTGGCGatg < 1:3151286/69‑1 (MQ=255) ccATTACTCAAGCCGCAGACTGGCGTTTGGCAACGGCACAGCTGCTGGAAATTGCAGGAGTTGGCGatg < 1:3215256/69‑1 (MQ=255) ccATTACTCAAGCCGCAGACTGGCGTTTGGCAACGGCACAGCTGCTGGAAATTGCAGGAGTTGGCGatg < 1:3359082/69‑1 (MQ=255) ccATTACTCAAGCCGCAGACTGGCGTTTGGCAACGGCACAGCTGCTGGAAATTGCAGGAGTTGGCGatg < 1:2000965/69‑1 (MQ=255) ccATTACTCAAGCCGCAGACTGGCGTTTGGCAACGGCACAGCTGCTGGAAATTGCAGGAGTTGGCGatg < 1:1868440/69‑1 (MQ=255) ccATTACTCAAGCCGCAGACTGGCGTTTGGCAACGGCACAGCTGCTGGAAATTGCAGGAGTTGGCGatg < 1:1354908/69‑1 (MQ=255) ccATTACTCAAGCCGCAGACTGGCGTTTGGCAACGGCACAGCTGCTGGAAATTGCAGGAGTTGGCGatg < 1:505551/69‑1 (MQ=255) ccATTACTCAAGCCGCAGACTGGCGTTTGGCAACGGCACAGCTGCTGGAAATTGCAGGAGTTGGCGatg < 1:538817/69‑1 (MQ=255) ccATTACTCAAGCCGCAGACTGGCGTTTGGCAACGGCACAGCTGCTGGAAATTGCAGGAGTTGGCGatg < 1:557259/69‑1 (MQ=255) ccATTACTCAAGCCGCAGACTGGCGTTTGGCAACGGCACAGCTGCTGGAAATTGCAGGAGTTGGCGatg < 1:272241/69‑1 (MQ=255) ccATTACTCAAGCCGCAGACTGGCGTTTGGCAACGGCACAGCTGCTGGAAATTGCAGGAGTTGGCGatg < 1:2386423/69‑1 (MQ=255) ccATTACTCAAGCCGCAGACTGGCGTTTGGCAACGGCACAGCTGCTGGAAATTGCAGGAGTTGGCGatg < 1:2836625/69‑1 (MQ=255) ccATTACTCAAGCCGCAGACTGGCGTTTGGCAACGGCACAGCTGCTGGAAATTGCAGGAGTTGGCGatg < 1:2824849/69‑1 (MQ=255) ccATTACTCAAGCCGCAGACTGGCGTTTGGCAACGGCACAGCTGCTGGAAATTGCAGGAGTTGGCGatg < 1:2703875/69‑1 (MQ=255) cATTACTCAAGCCGCAGACTGGCGTTTGGCAACGGCACAGCTGCTGGAAATTGCAGGAGTTGGCGatg < 1:2840418/68‑1 (MQ=255) ttACTCAAGCCGCAGACTGGCGTTTGGCAACGGCACAGCTGCTGGAAATTGCAGGAGTTGGCGatg < 1:1622211/66‑1 (MQ=255) tCAAGCCGCAGACTGGCGTTTGGCAACGGCACAGTTGCTGGAAATTGCAGGAGTTGGCGATGAATGAc < 1:133692/68‑1 (MQ=255) tCAAGCCGCAGACTGGCGTTTGGCAACGGCACAGTTGCTGGAAATTGCAGGAGTTGGCGATGAATGAc < 1:1354694/68‑1 (MQ=255) tCAAGCCGCAGACTGGCGTTTGGCAACGGCACAGTTGCTGGAAATTGCAGGAGTTGGCGATGAATGAc < 1:192862/68‑1 (MQ=255) cAAGCCGCAGACTGGCGTTTGGCAACGGCACAGTTGCTGGAAATTGCAGGAGTTGGCGATGAATGAc < 1:1598311/67‑1 (MQ=255) cAAGCCGCAGACTGGCGTTTGGCAACGGCACAGTTGCTGGAAATTGCAGGAGTTGGCGATGAATGAc < 1:2822502/67‑1 (MQ=255) cAAGCCGCAGACTGGCGTTTGGCAACGGCACAGTTGCTGGAAATTGCAGGAGTTGGCGATGAATGAc < 1:458221/67‑1 (MQ=255) gCAGACTGGCGTTTGGCAACGGCACAGTTGCTGGAAATTGCAGGAGTTGGCGATGAATGACCGTGACtt < 1:2157535/69‑1 (MQ=255) gCAGACTGGCGTTTGGCAACGGCACAGTTGCTGGAAATTGCAGGAGTTGGCGATGAATGACCGTGACtt < 1:1157547/69‑1 (MQ=255) gCAGACTGGCGTTTGGCAACGGCACAGTTGCTGGAAATTGCAGGAGTTGGCGATGAATGACCGTGACtt < 1:2586000/69‑1 (MQ=255) gCAGACTGGCGTTTGGCAACGGCACAGTTGCTGGAAATTGCAGGAGTTGGCGATGAATGACCGTGACtt < 1:714135/69‑1 (MQ=255) gCAGACTGGCGTTTGGCAACGGCACAGTTGCTGGAAATTGCAGGAGTTGGCGATGAATGACCGTGACtt < 1:513834/69‑1 (MQ=255) gCAGACTGGCGTTTGGCAACGGCACAGTTGCTGGAAATTGCAGGAGTTGGCGATGAATGACCGTGACtt < 1:720494/69‑1 (MQ=255) gCAGACTGGCGTTTGGCAACGGCACAGTTGCTGGAAATTGCAGGAGTTGGCGATGAATGACCGTGACtt < 1:813494/69‑1 (MQ=255) gCAGACTGGCGTTTGGCAACGGCACAGTTGCTGGAAATTGCAGGAGTTGGCGATGAATGACCGTGACtt < 1:490997/69‑1 (MQ=255) gCAGACTGGCGTTTGGCAACGGCACAGTTGCTGGAAATTGCAGGAGTTGGCGATGAATGACCGTGACtt < 1:1448007/69‑1 (MQ=255) cAGACTGGCGTTTGGCAACGGCACAGTTGCTGGAAATTGCAGGAGTTGGCGATGAATGACCGTGACtt < 1:1697834/68‑1 (MQ=255) cAGACTGGCGTTTGGCAACGGCACAGTTGCTGGAAATTGCAGGAGTTGGCGATGAATGACCGTGACtt < 1:2799061/68‑1 (MQ=255) cAGACTGGCGTTTGGCAACGGCACAGTTGCTGGAAATTGCAGGAGTTGGCGATGAATGACCGTGACtt < 1:3136755/68‑1 (MQ=255) ggCACAGTTGCTGGAAATTGCAGGAGTTGGCGATGAATGACCGTGACTTTATGCGTTATAGCCGCCaaa < 1:2792289/69‑1 (MQ=255) ggCACAGTTGCTGGAAATTGCAGGAGTTGGCGATGAATGACCGTGACTTTATGCGTTATAGCCGCCaaa < 1:2017439/69‑1 (MQ=255) ggCACAGTTGCTGGAAATTGCAGGAGTTGGCGATGAATGACCGTGACTTTATGCGTTATAGCCGCCaaa < 1:3023119/69‑1 (MQ=255) ggCACAGTTGCTGGAAATTGCAGGAGTTGGCGATGAATGACCGTGACTTTATGCGTTATAGCCGCCaaa < 1:1163731/69‑1 (MQ=255) gCACAGTTGCTGGAAATTGCAGGAGTTGGCGATGAATGACCGTGACtt < 1:44224/48‑1 (MQ=255) | GGCAGTATCGCCGTCGTCAGCGCCATTACTCAAGCCGCAGACTGGCGTTTGGCAACGGCACAGTTGCTGGAAATTGCAGGAGTTGGCGATGAATGACCGTGACTTTATGCGTTATAGCCGCCAAA > minE/2248723‑2248847 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |