| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,250,566 | C→T | 54.5% | I16I (ATC→ATT) | thiH → | thiamin biosynthesis ThiGH complex subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,250,566 | 0 | C | T | 54.5% | 3.8 / 54.7 | 66 | I16I (ATC→ATT) | thiH | thiamin biosynthesis ThiGH complex subunit |
| Reads supporting (aligned to +/- strand): ref base C (23/7); new base T (24/12); total (47/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.23e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.59e-01 | |||||||||||
GGCATCGGCATGAAAACCTTCAGCGATCGCTGGCGACAACTGGACTGGGACGACATCCGCCTGCGTATCAACGGCAAAACGGCTGCTGACGTAGAGCGGGCGCTAAATGCCTCGCAACT > minE/2250510‑2250628 | ggCATCGGCATGAAAACCTTCGGCGATCGCTGGCGACAACTGGACTGGGACGACATTCGCCTGCGTATc < 1:232378/69‑1 (MQ=255)ggCATCGGCATGAAAACCTTCAGCGATCGCTGGCGACAACTGGACTGGGACGACATTCGCCTGCGTATc < 1:991789/69‑1 (MQ=255)ggCATCGGCATGAAAACCTTCAGCGATCGCTGGCGACAACTGGACTGGGACGACATTCGCCTGCGTATc < 1:2460125/69‑1 (MQ=255)ggCATCGGCATGAAAACCTTCAGCGATCGCTGGCGACAACTGGACTGGGACGACATTCGCCTGCGTATc < 1:1749148/69‑1 (MQ=255)ggCATCGGCATGAAAACCTTCAGCGATCGCTGGCGACAACTGGACTGGGACGACATTCGCCTGCGTATc < 1:1700952/69‑1 (MQ=255)ggCATCGGCATGAAAACCTTCAGCGATCGCTGGCGACAACTGGACTGGGACGACATTCGCCTGCGTATc < 1:3388591/69‑1 (MQ=255)ggCATCGGCATGAAAACCTTCAGCGATCGCTGGCGACAACTGGACTGGGACGACATTCGCCTGCGTATc < 1:47745/69‑1 (MQ=255)ggCATCGGCATGAAAACCTTCAGCGATCGCTGGCGACAACTGGACTGGGACGACATTCGCCTGCGTATc < 1:560113/69‑1 (MQ=255)ggCATCGGCATGAAAACCTTCAGCGATCGCTGGCGACAACTGGACTGGGACGACATTCGCCTGCGTATc < 1:139545/69‑1 (MQ=255)ggCATCGGCATGAAAACCTTCAGCGATCGCTGGCGACAACTGGACTGGGACGACATTCGCCTGCGTATc < 1:946597/69‑1 (MQ=255) cATCGGCATGAAAACCTTCAGCGATCGCTGGCGACAACTGGACTGGGACGACATTCGCCTGCGTATc < 1:2919074/67‑1 (MQ=255) ggCATGAAAACCTTCAGCGATCGCTGGCGACAACTGGACTGGGACGACATCCGCCTGCGTATCAACgg > 1:1437042/1‑68 (MQ=255) ggCATGAAAACCTTCAGCGATCGCTGGCGACAACTGGACTGGGACGACATCCGCCTGCGTATCAACgg > 1:2793335/1‑68 (MQ=255) ggCATGAAAACCTTCAGCGATCGCTGGCGACAACTGGACTGGGACGACATCCGCCTGCGTATCAACgg > 1:2714004/1‑68 (MQ=255) ggCATGAAAACCTTCAGCGATCGCTGGCGACAACTGGACTGGGACGACATCCGCCTGCGTATCAACg > 1:3339256/1‑67 (MQ=255) ggCATGAAAACCTTCAGCGATCGCTGGCGACAACTGGACTGGGACGACATCCGCCTGCGTATCAACg > 1:3335022/1‑67 (MQ=255) aaaaCCTTCAGCGATCGCTGGCGACAACTGGACTGGGACGACATcc < 1:3404456/46‑1 (MQ=255) aaaaCCTTCAGCGATCGCTGGCGACAACTGGACTGGGACGACATcc < 1:2980032/46‑1 (MQ=255) aaaaCCTTCAGCGATCGCTGGCGACAACTGGACTGGGACGACATCCGCCTGCGTATc < 1:2733630/57‑1 (MQ=255) aaaaCCTTCAGCGATCGCTGGCGACAACTGGACTGGGACGACATCCGCCTGCGTATc < 1:2675802/57‑1 (MQ=255) aaaaCCTTCAGCGATCGCTGGCGACAACTGGACTGGGACGACATCCGCCTGCGTATc < 1:2826217/57‑1 (MQ=255) cgatGGCGACAACTGGACTGGGACGACATCCGCCTGCGTATCAACGGCAAAACGGCTGCTGAGGTagag > 1:1683500/4‑69 (MQ=255) cGCTGGCGACAACTGGACTGGGACGACATCCGCCTGCGTATCAACGGCAAAACGGCTGCTGCCGTagc > 1:369539/1‑67 (MQ=255) cGCTGGCGACAACTGGACTGGGACGACATCCGCCTGCGTATCAACGGCAAAACGGCTGCTGACGTagag > 1:1591575/1‑69 (MQ=255) cGCTGGCGACAACTGGACTGGGACGACATCCGCCTGCGTATCAACGGCAAAACGGCTGCTGACGTagag > 1:3309131/1‑69 (MQ=255) cGCTGGCGACAACTGGACTGGGACGACATCCGCCTGCGTATCAACGGCAAAACGGCTGCTGACGTagag > 1:1942964/1‑69 (MQ=255) cGCTGGCGACAACTGGACTGGGACGACATCCGCCTGCGTATCAACGGCAAAACGGCTGCTGACGTagag > 1:724567/1‑69 (MQ=255) cGCTGGCGACAACTGGACTGGGACGACATCCGCCTGCGTATCAACGGCAAAACGGCTGCTGACGTagag > 1:1687910/1‑69 (MQ=255) cGCTGGCGACAACTGGACTGGGACGACATCCGCCTGCGTATCAACGGCAAAACGGCTGCTGACGTagag > 1:927874/1‑69 (MQ=255) ggCTACAACTGGACTGGGACGACATCCGCCTGCGTATc < 1:3300125/38‑1 (MQ=255) ggCGACAACTGGACTGGGACGACATCCGCCTGCGTATCAACGGCAAAACGGCTGCTGACGTAGAGCggg < 1:3104559/69‑1 (MQ=255) gACAACTGGACTGGCACGACATTCGCCTGCGTATc < 1:2183571/35‑1 (MQ=38) cAACTGGACTGGGACGACATCCGCCTGCGTATCAACGGCAAAACGGCTGCTGACGTAGAGCGGGCGCTa < 1:1745478/69‑1 (MQ=255) aaCTGGACTGGGACGACATCCGCCTGCGTATCAACGGCAAAACGGCTGCTGACGTAGAGCGGGCGCTa < 1:1511196/68‑1 (MQ=255) tggactggGACGACATCCGCCTGCGTATCAACGGCAAAACGGCTGCTGACGTAGAGCGGGCGCTAAATg > 1:149274/1‑69 (MQ=255) ggACGACATCCGCCTGCGTTTCAACGGCAAAACGGCTGCTGACGTAGAGCGGGCGCTAAATGCCTCGCa > 1:3208241/1‑69 (MQ=255) ggACGACATCCGCCTGCGTATCAACGGCAAAACGGCTGCTGACGTAGAGCGGGCGCTAAATGCCTCGCa > 1:1722271/1‑69 (MQ=255) ggACGACATCCGCCTGCGTATCAACGGCAAAACGGCTGCTGACGTAGAGCGGGCGCTAAATGCCTCGCa > 1:922033/1‑69 (MQ=255) ggACGACATCCGCCTGCGTATCAACGGCAAAACGGCTGCTGACGTAGAGCGGGCGCTAAATGCCTCGCa > 1:1937857/1‑69 (MQ=255) ggACGACATCCGCCTGCGTATCAACGGCAAAACGGCTGCTGACGTAGAGCGGGCGCTAAATGCCTCGCa > 1:2706997/1‑69 (MQ=255) ggACGACATCCGCCTGCGTATCAACGGCAAAACGGCTGCTGACGTAGAGCGGGCGCTAAATGCCTCGCa > 1:38993/1‑69 (MQ=255) ggACGACATCCGCCTGCGTATCAACGGCAAAACGGCTGCTGACGTAGAGCGGGCGCTAAATGCCTCGCa > 1:2449932/1‑69 (MQ=255) ggACGACATCCGCCTGCGTATCAACGGCAAAACGGCTGCTGACGTAGAGCGGGCGCTAAATGCCTCGCa > 1:3328447/1‑69 (MQ=255) ggACGACATCCGCCTGCGTATCAACGGCAAAACGGCTGCTGACGTAGAGCGGGCGCTAAATGCCTCGCa > 1:2385118/1‑69 (MQ=255) cgacATTCGCCTGCGTATCAACAGCAAAACGGCTGCTGACGTAGAGCGGgcg > 1:2282388/1‑52 (MQ=255) cgacATTCGCCTGCGTATCAACAGCAAAACGGCTGCTGACGTAGAGCGGGCGCTAAATGCCTCGCAAc > 1:3037849/1‑68 (MQ=255) cgacATTCGCCTGCGTATCAACAGCAAAACGGCTGCTGACGTAGAGCGGGCGCTAAATGCCTCGCAACt > 1:1096384/1‑69 (MQ=255) cgacATTCGCCTGCGTATCAACAGCAAAACGGCTGCTGACGTAGAGCGGGCGCTAAATGCCTCGCAACt > 1:553437/1‑69 (MQ=255) cgacATTCGCCTGCGTATCAACAGCAAAACGGCTGCTGACGTAGAGCGGGCGCTAAATGCCTCGCAACt > 1:976883/1‑69 (MQ=255) cgacATTCGCCTGCGTATCAACAGCAAAACGGCTGCTGACGTAGAGCGGGCGCTAAATGCCTCGCAACt > 1:1172123/1‑69 (MQ=255) cgacATTCGCCTGCGTATCAACAGCAAAACGGCTGCTGACGTAGAGCGGGCGCTAAATGCCTCGCAACt > 1:577495/1‑69 (MQ=255) cgacATTCGCCTGCGTATCAACAGCAAAACGGCTGCTGACGTAGAGCGGGCGCTAAATGCCTCGCAACt > 1:642352/1‑69 (MQ=255) cgacATTCGCCTGCGTATCAACAGCAAAACGGCTGCTGACGTAGAGCGGGCGCTAAATGCCTCGCAACt > 1:648636/1‑69 (MQ=255) cgacATTCGCCTGCGTATCAACAGCAAAACGGCTGCTGACGTAGAGCGGGCGCTAAATGCCTCGCAACt > 1:93379/1‑69 (MQ=255) cgacATTCGCCTGCGTATCAACAGCAAAACGGCTGCTGACGTAGAGCGGGCGCTAAATGCCTCGCAACt > 1:910262/1‑69 (MQ=255) cgacATTCGCCTGCGTATCAACAGCAAAACGGCTGCTGACGTAGAGCGGGCGCTAAATGCCTCGCAACt > 1:1544848/1‑69 (MQ=255) cgacATTCGCCTGCGTATCAACAGCAAAACGGCTGCTGACGTAGAGCGGGCGCTAAATGCCTCGCAACt > 1:165974/1‑69 (MQ=255) cgacATTCGCCTGCGTATCAACAGCAAAACGGCTGCTGACGTAGAGCGGGCGCTAAATGCCTCGCAACt > 1:3282443/1‑69 (MQ=255) cgacATTCGCCTGCGTATCAACAGCAAAACGGCTGCTGACGTAGAGCGGGCGCTAAATGCCTCGCAACt > 1:3242929/1‑69 (MQ=255) cgacATTCGCCTGCGTATCAACAGCAAAACGGCTGCTGACGTAGAGCGGGCGCTAAATGCCTCGCAACt > 1:3202005/1‑69 (MQ=255) cgacATTCGCCTGCGTATCAACAGCAAAACGGCTGCTGACGTAGAGCGGGCGCTAAATGCCTCGCAACt > 1:2943952/1‑69 (MQ=255) cgacATTCGCCTGCGTATCAACAGCAAAACGGCTGCTGACGTAGAGCGGGCGCTAAATGCCTCGCAACt > 1:1004737/1‑69 (MQ=255) cgacATTCGCCTGCGTATCAACAGCAAAACGGCTGCTGACGTAGAGCGGGCGCTAAATGCCTCGCAACt > 1:1827721/1‑69 (MQ=255) cgacATTCGCCTGCGTATCAACAGCAAAACGGCTGCTGACGTAGAGCGGGCGCTAAATGCCTCGCAACt > 1:2135487/1‑69 (MQ=255) cgacATTCGCCTGCGTATCAACAGCAAAACGGCTGCTGACGTAGAGCGGGCGCTAAATGCCTCGCAACt > 1:2418421/1‑69 (MQ=255) cgacATTCGCCTGCGTATCAACAGCAAAACGGCTGCTGACGTAGAGCGGGCGCTAAATGCCTCGCAACt > 1:2152931/1‑69 (MQ=255) cgacATTCGCCTGCGTATCAACAGCAAAACGGCTGCTGACGTAGAGCGGGCGCTAAATGCCTCGCAACt > 1:2322237/1‑69 (MQ=255) cgacATTCGCCTGCGTATCAACAGCAAAACGGCTGCTGACGTAGAGCCGGCGCTAAATGCCTCGCAACt > 1:3032552/1‑69 (MQ=255) | GGCATCGGCATGAAAACCTTCAGCGATCGCTGGCGACAACTGGACTGGGACGACATCCGCCTGCGTATCAACGGCAAAACGGCTGCTGACGTAGAGCGGGCGCTAAATGCCTCGCAACT > minE/2250510‑2250628 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |