| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 26,138 | G→A | 100% | E29E (GAG→GAA) | dapB → | dihydrodipicolinate reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 26,138 | 0 | G | A | 81.4% | 18.9 / ‑0.9 | 16 | E29E (GAG→GAA) | dapB | dihydrodipicolinate reductase |
| Reads supporting (aligned to +/- strand): ref base G (2/1); new base A (0/13); total (2/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.50e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.71e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGTGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTT > minE/26095‑26181 | gccGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAAGGCGTGCAGCTGGGCGCGGCGCTgg < 1:1279149/67‑1 (MQ=255)gccGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAAGGCGTGCAGCTGGGCGCGGCGCTgg < 1:1741985/67‑1 (MQ=255)gccGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAAGGCGTGCAGCTGGGCGCGGCGCTgg < 1:1774790/67‑1 (MQ=255)gccGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAAGGCGTGCAGCTGGGCGCGGCGCTgg < 1:2168348/67‑1 (MQ=255)gccGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAAGGCGTGCAGCTGGGCGCGGCGCTgg < 1:2502928/67‑1 (MQ=255)gccGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAAGGCGTGCAGCTGGGCGCGGCGCTgg < 1:2893762/67‑1 (MQ=255)gccGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAAGGCGTGCAGCTGGGCGCGGCGCTgg < 1:3046238/67‑1 (MQ=255)gccGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAAGGCGTGCAGCTGGGCGCGGCGCTgg < 1:3324599/67‑1 (MQ=255)gccGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAAGGCGTGCAGCTGGGCGCGGCGCTgg < 1:3330485/67‑1 (MQ=255)gccGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAAGGCGTGCAGCTGGGCGCGGCGCTgg < 1:357367/67‑1 (MQ=255)gccGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAAGGCGTGCAGCTGGGCGCGGCGCTgg < 1:620110/67‑1 (MQ=255)gccGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAAGGCGTGCAGCTGGGCGCGGCGCTgg < 1:869592/67‑1 (MQ=255) ccGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAAGGCGTGCAGCTGGGCGCGGCGCTgg < 1:467465/67‑1 (MQ=255) tGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGTGCAGTTGGGCGCTGCGCTGGAGCg > 1:513164/1‑67 (MQ=255) ggCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGTGCAGTTGGGCGCTGCGCTGGAGCGTGa > 1:3416713/1‑68 (MQ=255) tGATTCAGGCGGCGCTGGCATTAGAGGGCGTGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATcttctt < 1:56225/69‑1 (MQ=255) | GGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGTGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTT > minE/26095‑26181 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |