| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,251,037 | T→A | 51.8% | L173L (CTT→CTA) | thiH → | thiamin biosynthesis ThiGH complex subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,251,037 | 0 | T | A | 51.8% | 6.6 / 139.0 | 114 | L173L (CTT→CTA) | thiH | thiamin biosynthesis ThiGH complex subunit |
| Reads supporting (aligned to +/- strand): ref base T (14/41); new base A (54/5); total (68/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.36e-13 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01 | |||||||||||
Only 100 of 114 total aligned reads displayed.
TCTCTTCACTACAGATGGAAGTGCAACCGCTGGCGGAGACGGAATACGCCGAGTTAAAGCAACTTGGTCTGGATGGCGTGATGGTTTATCAGGAGACATATCACGAGGCGACTTATGCCCGCC > minE/2250973‑2251095 | tctcTTCACTACAGATGGAAGTGCAACCGCTGGCGGAGACGGAATACGCCGAGTTAAAGCAACTTGGTc > 1:1030489/1‑69 (MQ=255) cACTACAGATGGAAGTGCAACCGCTGGCGGAGACGGAATACGCCGAGTTAAAGCAACTTGGTCTGGATg > 1:1140820/1‑69 (MQ=255) cACTACAGATGGAAGTGCAACCGCTGGCGGAGACGGAATACGCCGAGTTAAAGCAACTTGGTCTGGATg > 1:241661/1‑69 (MQ=255) cACTACAGATGGAAGTGCAACCGCTGGCGGAGACGGAATACGCCGAGTTAAAGCAACTTGGTCTGGATg > 1:3112248/1‑69 (MQ=255) aGTGCAACCGCTGGCGGAGACGGAATACGCCGAGTTAAAGCAACTTGGTCTGGATGGCGTGATGGttt > 1:2711151/1‑68 (MQ=255) aGTGCAACCGCTGGCGGAGACGGAATACGCCGAGTTAAAGCAACTTGGTCTGGATGGCGTGATGGttt > 1:164356/1‑68 (MQ=255) aGTGCAACCGCTGGCGGAGACGGAATACGCCGAGTTAAAGCAACTTGGTCTGGATGGCGTGATGGttt > 1:1495427/1‑68 (MQ=255) cAACCGCTGGCGGAGACGGAATACGCCGAGTTAAAGCAACTTGGTCTGGATGGCGTGATGGTTTATc > 1:2866419/1‑67 (MQ=255) cAACCGCTGGCGGAGACGGAATACGCCGAGTTAAAGCAACTTGGTCTGGATGGCGTGATGGTTTATc > 1:2151478/1‑67 (MQ=255) cAACCGCTGGCGGAGACGGAATACGCCGAGTTAAAGCAACTTGGTCTGGATGGCGTGATGGTTTATc > 1:1362540/1‑67 (MQ=255) cAACCGCTGGCGGAGACGGAATACGCCGAGTTAAAGCAACTAGGTCTGGATGGCGTGATGGTTTATc < 1:1901006/67‑1 (MQ=255) cAACCGCTGGCGGAGACGGAATACGCCGAGTTAAAGCAACTAGGTCTGGATGGCGTGATGGTTTATc < 1:3429530/67‑1 (MQ=255) cAACCGCTGGCGGAGACGGAATACGCCGAGTTAAAGCAACTAGGTCTGGATGGCGTGATGGTTTATc < 1:443998/67‑1 (MQ=255) cAACCGCTGGCGGAGACGGAATACGCCGAGTTAAAGCAACTAGGTCTGGATGGCGTGATGGTTTATc < 1:1123393/67‑1 (MQ=255) cAACCGCTGGCGGAGACGGAATACGCCGAGTTAAAGCAACTAGGTCTGGATGGCGTGATGGTTTATc < 1:742598/67‑1 (MQ=255) cGCTGGCGGAGACGGAATACGCCGAGTTAAAGCAACTTGGTCTGGATGGCGTGATGGTTTATCAGgaga < 1:2600950/69‑1 (MQ=255) cGCTGGCGGAGACGGAATACGCCGAGTTAAAGCAACTTGGTCTGGATGGCGTGATGGTTTATCAGgaga < 1:3140529/69‑1 (MQ=255) cGCTGGCGGAGACGGAATACGCCGAGTTAAAGCAACTTGGTCTGGATGGCGTGATGGTTTATCAGgaga < 1:2273343/69‑1 (MQ=255) cGCTGGCGGAGACGGAATACGCCGAGTTAAAGCAACTTGGTCTGGATGGCGTGATGGTTTATCAGgaga < 1:1202534/69‑1 (MQ=255) cGCTGGCGGAGACGGAATACGCCGAGTTAAAGCAACTTGGTCTGGATGGCGTGATGGTTTATCAGgaga < 1:2478656/69‑1 (MQ=255) cGCTGGCGGAGACGGAATACGCCGAGTTAAAGCAACTTGGTCTGGATGGCGTGATGGTTTATCAGgaga < 1:3090842/69‑1 (MQ=255) cGCTGGCGGAGACGGAATACGCCGAGTTAAAGCAACTTGGTCTGGATGGCGTGATGGTTTATCAGgaga < 1:210043/69‑1 (MQ=255) cGCTGGCGGAGACGGAATACGCCGAGTTAAAGCAACTTGGTCTGGATGGCGTGATGGTTTATCAGgaga < 1:367527/69‑1 (MQ=255) cGCTGGCGGAGACGGAATACGCCGAGTTAAAGCAACTTGGTCTGGATGGCGTGATGGTTTATCAGgaga < 1:2738640/69‑1 (MQ=255) cGCTGGCGGAGACGGAATACGCCGAGTTAAAGCAACTTGGTCTGGATGGCGTGATGGTTTATCAGgaga < 1:372879/69‑1 (MQ=255) cGCTGGCGGAGACGGAATACGCCGAGTTAAAGCAACTTGGTCTGGATGGCGTGATGGTTTATCAGgaga < 1:820901/69‑1 (MQ=255) cGCTGGCGGAGACGGAATACGCCGAGTTAAAGCAACTTGGTCTGGATGGCGTGATGGTTTATCAGgaga < 1:51257/69‑1 (MQ=255) cGCTGGCGGAGACGGAATACGCCGAGTTAAAGCAACTTGGTCTGGATGGCGTGATGGTTTATCAGgaga < 1:1057415/69‑1 (MQ=255) cGCTGGCGGAGACGGAATACGCCGAGTTAAAGCAACTTGGTCTGGATGGCGTGATGGTTTATCAGgaga < 1:1416267/69‑1 (MQ=255) tGGCGGAGACGGAATACGCCGAGTTAAAGCAACTTGGTCTGGATGGCGTGATGGTTTATCAGGAGACat < 1:2899815/69‑1 (MQ=255) tGGCGGAGACGGAATACGCCGAGTTAAAGCAACTTGGTCTGGATGGCGTGATGGTTTATCAGGAGACat < 1:180665/69‑1 (MQ=255) tGGCGGAGACGGAATACGCCGAGTTAAAGCAACTTGGTCTGGATGGCGTGATGGTTTATCAGGAGACat < 1:1711817/69‑1 (MQ=255) tGGCGGAGACGGAATACGCCGAGTTAAAGCAACTTGGTCTGGATGGCGTGATGGTTTATCAGGAGACat < 1:2651343/69‑1 (MQ=255) tGGCGGAGACGGAATACGCCGAGTTAAAGCAACTTGGTCTGGATGGCGTGATGGTTTATCAGGAGACat < 1:45597/69‑1 (MQ=255) tGGCGGAGACGGAATACGCCGAGTTAAAGCAACTTGGTCTGGATGGCGTGATGGTTTATCAGGAGACat < 1:3311270/69‑1 (MQ=255) tGGCGGAGACGGAATACGCCGAGTTAAAGCAACTTGGTCTGGATGGCGTGATGGTTTATCAGGAGACat < 1:2268931/69‑1 (MQ=255) tGGCGGAGACGGAATACGCCGAGTTAAAGCAACTTGGTCTGGATGGCGTGATGGTTTATCAGGAGACat < 1:2311831/69‑1 (MQ=255) tGGCGGAGACGGAATACGCCGAGTTAAAGCAACTTGGTCTGGATGGCGTGATGGTTTATCAGGAGACat < 1:919348/69‑1 (MQ=255) ggCGGAGACGGAATACGCCGAGTTAAAGCAACTTGGTCTGGATGGCGTGATGGTTTATCAGGAGACat < 1:357679/68‑1 (MQ=255) ggCGGAGACGGAATACGCCGAGTTAAAGCAACTTGGTCTGGATGGCGTGATGGTTTATCAGGAGACat < 1:918374/68‑1 (MQ=255) ggCGGAGACGGAATACGCCGAGTTAAAGCAACTTGGTCTGGATGGCGTGATGGTTTATCAGGAGACat < 1:1914470/68‑1 (MQ=255) ggAGACGGAATACGCCGAGTTAAAGCACCTAGGTCTGGATGGCGTGATGGTTTATCAGGAGACATATc > 1:3362588/1‑68 (MQ=255) ggAGACGGAATACGCCGAGTTAAAGCAACTAGGTCTGGATGGCGTGATGGTTTATCAGGAGACATATc > 1:2687371/1‑68 (MQ=255) ggAGACGGAATACGCCGAGTTAAAGCAACTAGGTCTGGATGGCGTGATGGTTTATCAGGAGACATATc > 1:727829/1‑68 (MQ=255) ggAGACGGAATACGCCGAGTTAAAGCAACTAGGTCTGGATGGCGTGATGGTTTATCAGGAGACATATc > 1:3164850/1‑68 (MQ=255) ggAGACGGAATACGCCGAGTTAAAGCAACTAGGTCTGGATGGCGTGATGGTTTATCAGGAGACATATc > 1:901205/1‑68 (MQ=255) ggAGACGGAATACGCCGAGTTAAAGCAACTAGGTCTGGATGGCGTGATGGTTTATCAGGAGACATATc > 1:2689701/1‑68 (MQ=255) ggAGACGGAATACGCCGAGTTAAAGCAACTAGGTCTGGATGGCGTGATGGTTTATCAGGAGACATATc > 1:52144/1‑68 (MQ=255) ggAGACGGAATACGCCGAGTTAAAGCAACTAGGTCTGGATGGCGTGATGGTTTATCAGGAGACATATc > 1:527641/1‑68 (MQ=255) ggAGACGGAATACGCCGAGTTAAAGCAACTAGGTCTGGATGGCGTGATGGTTTATCAGGAGACATATc > 1:2143123/1‑68 (MQ=255) ggAGACGGAATACGCCGAGTTAAAGCAACTAGGTCTGGATGGCGTGATGGTTTATCAGGAGACATATc > 1:1640974/1‑68 (MQ=255) ggAGACGGAATACGCCGAGTTAAAGCAACTAGGTCTGGATGGCGTGATGGTTTATCAGGAGACATATc > 1:2350928/1‑68 (MQ=255) ggAGACGGAATACGCCGAGTTAAAGCAACTAGGTCTGGATGGCGTGATGGTTTATCAGGAGACATATc > 1:1626731/1‑68 (MQ=255) ggAGACGGAATACGCCGAGTTAAAGCAACTAGGTCTGGATGGCGTGATGGTTTATCAGGAGACATATc > 1:1270174/1‑68 (MQ=255) ggAGACGGAATACGCCGAGTTAAAGCAACTAGGTCTGGATGGCGTGATGGTTTATCAGGAGACATATc > 1:2296121/1‑68 (MQ=255) ggAGACGGAATACGCCGAGTTAAAGAAACTTGGTCTGGATGGCGTGATGGTTTATCAGGAGACATATc > 1:1212011/1‑68 (MQ=255) ggAATACGCCGAGTTAAAGCAACTTGGTCTGGATGGCGTGATgg < 1:704109/44‑1 (MQ=255) ggAATACGCCGAGTTAAAGCAACTTGGTCTGGATGGCGTGATgg < 1:2637695/44‑1 (MQ=255) ggAATACGCCGAGTTAAAGCAACTTGGTCTGGATGGCGTGATgg < 1:449162/44‑1 (MQ=255) tACGCCGAGTTAAAGCAACTTGGTCTGGATGGCGTGATGGTTTATCAGGAGACATATCACGAGGCGACt < 1:92088/69‑1 (MQ=255) tACGCCGAGTTAAAGCAACTTGGTCTGGATGGCGTGATGGTTTATCAGGAGACATATCACGAGGCGACt < 1:2891762/69‑1 (MQ=255) tACGCCGAGTTAAAGCAACTTGGTCTGGATGGCGTGATGGTTTATCAGGAGACATATCACGAGGCGACt < 1:3056579/69‑1 (MQ=255) tACGCCGAGTTAAAGCAACTTGGTCTGGATGGCGTGACGGTTTATCAGGAGACATATCACGAGGCGACt < 1:412738/69‑1 (MQ=255) aCGCCGAGTTAAAGCAACTTGGTCTGGATGGCGTGATGGTTTATCAGGAGACATATCACGAGGCGACt < 1:2654145/68‑1 (MQ=255) aCGCCGAGTTAAAGCAACTTGGTCTGGATGGCGTGATGGTTTATCAGGAGACATATCACGAGGCGACt < 1:214375/68‑1 (MQ=255) gAGTTAAAGCAACTTGGTCTGGATGGCGTGATGGTTTATCAGGAGACATATCACGAGGCGACTTAt < 1:1873096/66‑1 (MQ=255) tAAAGCAACTTGGTCTGGATGGCGTGATGGTTTATCAGGAGACATATCACGAGGCGACTTATGCCGGcc < 1:2076189/69‑1 (MQ=255) tAAAGCAACTTGGTCTGGATGGCGTGATGGTTTATCAGGAGACATATCACGAGGCGACTTATGCCCGcc < 1:480671/69‑1 (MQ=255) tAAAGCAACTAGGTCTGTATGGCGTGATGGTTTATCAGGAGACATATCACGAGGCGACtt > 1:3401432/1‑60 (MQ=255) tAAAGCAACTAGGTCTGGATGGCGTGATGGTTTATCAGGAGACATATCACGAGGCGACtt > 1:3432822/1‑60 (MQ=255) tAAAGCAACTAGGTCTGGATGGCGTGATGGTTTATCAGGAGACATATCACGAGGCGACtt > 1:175704/1‑60 (MQ=255) tAAAGCAACTAGGTCTGGATGGCGTGATGGTTTATCAGGAGACATATCACGAGGCGACtt > 1:478669/1‑60 (MQ=255) tAAAGCAACTAGGTCTGGATGGCGTGATGGTTTATCAGGAGACATATCACGAGGCGACtt > 1:899120/1‑60 (MQ=255) tAAAGCAACTAGGTCTGGATGGCGTGATGGTTTATCAGGAGACATATCACGAGGCGACtt > 1:928183/1‑60 (MQ=255) tAAAGCAACTAGGTCTGGATGGCGTGATGGTTTATAAGGAGACATATCACGAGGCGACtt > 1:1023044/1‑60 (MQ=255) aaaGCAACTAGGTCTGGATGGCGTGATGGTTTATCAGGGGACATATCACGAGGCGAc > 1:2829095/1‑57 (MQ=255) aaaGCAACTAGGTCTGGATGGCGTGATGGTTTATCAGGAGACATATCACGAGGCGAc > 1:1441233/1‑57 (MQ=255) aaaGCAACTAGGTCTGGATGGCGTGATGGTTTATCAGGAGACATATCACGAGGCGAc > 1:985736/1‑57 (MQ=255) aaaGCAACTAGGTCTGGATGGCGTGATGGTTTATCAGGAGACATATCACGAGGCGAc > 1:2665798/1‑57 (MQ=255) aaaGCAACTAGGTCTGGATGGCGTGATGGTTTATCAGGAGACATATCACGAGGCGAc > 1:2661699/1‑57 (MQ=255) aaaGCAACTAGGTCTGGATGGCGTGATGGTTTATCAGGAGACATATCACGAGGCGAc > 1:1229040/1‑57 (MQ=255) aaaGCAACTAGGTCTGGATGGCGTGATGGTTTATCAGGAGACATATCACGAGGCGAc > 1:1259503/1‑57 (MQ=255) aaaGCAACTAGGTCTGGATGGCGTGATGGTTTATCAGGAGACATATCACGAGGCGAc > 1:2788227/1‑57 (MQ=255) aaaGCAACTAGGTCTGGATGGCGTGATGGTTTATCAGGAGACATATCACGAGGCGAc > 1:751337/1‑57 (MQ=255) aaaGCAACTAGGTCTGGATGGCGTGATGGTTTATCAGGAGACATATCACGAGGCGAc > 1:1435337/1‑57 (MQ=255) aaaGCAACTAGGTCTGGATGGCGTGATGGTTTATCAGGAGACATATCACGAGGCGAc > 1:2126027/1‑57 (MQ=255) aaaGCAACTAGGTCTGGATGGCGTGATGGTTTATCAGGAGACATATCACGAGGCGAc > 1:1621257/1‑57 (MQ=255) aaaGCAACTAGGTCTGGATGGCGTGATGGTTTATCAGGAGACATATCACGAGGCGAc > 1:2831993/1‑57 (MQ=255) aaaGCAACTAGGTCTGGATGGCGTGATGGTTTATCAGGAGACATATCACGAGGCGAc > 1:2870720/1‑57 (MQ=255) aaaGCAACTAGGTCTGGATGGCGTGATGGTTTATCAGGAGACATATCACGAGGCGAc > 1:2877407/1‑57 (MQ=255) aaaGCAACTAGGTCTGGATGGCGTGATGGTTTATCAGGAGACATATCACGAGGCGAc > 1:2513469/1‑57 (MQ=255) aaaGCAACTAGGTCTGGATGGCGTGATGGTTTATCAGGAGACATATCACGAGGCGAc > 1:2935064/1‑57 (MQ=255) aaaGCAACTAGGTCTGGATGGCGTGATGGTTTATCAGGAGACATATCACGAGGCGAc > 1:2498581/1‑57 (MQ=255) aaaGCAACTAGGTCTGGATGGCGTGATGGTTTATCAGGAGACATATCACGAGGCGAc > 1:2400375/1‑57 (MQ=255) aaaGCAACTAGGTCTGGATGGCGTGATGGTTTATCAGGAGACATATCACGAGGCGAc > 1:380713/1‑57 (MQ=255) aaaGCAACTAGGTCTGGATGGCGTGATGGTTTATCAGGAGACATATCACGAGGCGAc > 1:2215432/1‑57 (MQ=255) aaaGCAACTAGGTCTGGATGGCGTGATGGTTTATCAGGAGACATATCACGAGGCGAc > 1:3348051/1‑57 (MQ=255) aaaGCAACTAGGTCTGGATGGCGTGATCGTTTATCAg > 1:276693/1‑37 (MQ=37) aaaGCAACTAGGTCTGCATGGCGTGATGGTTTATCAGGAGACATATCACGAGGCGAc > 1:2767072/1‑57 (MQ=255) aaaGCAACTAGGTCAGGATGGCGTGATGGTTTATCAGGAGACATATCa > 1:1363314/1‑48 (MQ=39) | TCTCTTCACTACAGATGGAAGTGCAACCGCTGGCGGAGACGGAATACGCCGAGTTAAAGCAACTTGGTCTGGATGGCGTGATGGTTTATCAGGAGACATATCACGAGGCGACTTATGCCCGCC > minE/2250973‑2251095 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |