| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,251,648 | T→C | 27.3% | L377P (CTA→CCA) | thiH → | thiamin biosynthesis ThiGH complex subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,251,648 | 0 | T | C | 27.3% | 75.8 / 28.9 | 73 | L377P (CTA→CCA) | thiH | thiamin biosynthesis ThiGH complex subunit |
| Reads supporting (aligned to +/- strand): ref base T (34/19); new base C (9/11); total (43/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.84e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAGGGTTTGCAGCCGGTATGGAAAGACTGGGACAGCTATCTGGGACGCGCCTCGCAAAGACTATGAGACGGAATGTAAGAACGTAAATTTATTCCGAGCCGCTACGGAAGATGAAAAACGCAAGGTTG > minE/2251587‑2251714 | cAGGGTTTGCAGCCGGTATGGAAAGACTGGGACAGCTATCTGGGACGCGCCTCGCAAAGACTATgaga > 1:947673/1‑68 (MQ=255)cAGGGTTTGCAGCCGGTATGGAAAGACTGGGACAGCTATCTGGGACGCGCCTCGCAAAGACTATgaga > 1:679384/1‑68 (MQ=255) ggTTTGCAGCCGGTATGGAAAGACTGGGACAGCTATCTGGGACGCGCCTCGCAAAGACTATGAGACgg < 1:1217639/68‑1 (MQ=255) ggTTTGCAGCCGGTATGGAAAGACTGGGACAGCTATCTGGGACGCGCCTCGCAAAGACTATGAGACgg < 1:1599763/68‑1 (MQ=255) ggTTTGCAGCCGGTATGGAAAGACTGGGACAGCTATCTGGGACGCGCCTCGCAAAGACTATGAGACgg < 1:1398720/68‑1 (MQ=255) ggTTTGCAGCCGGTATGGAAAGACTGGGACAGCTATCTGGGACGCGCCTCGCAAAGACTATGAGACgg < 1:1829690/68‑1 (MQ=255) ggTTTGCAGCCGGTATGGAAAGACTGGGACAGCTATCTGGGACGCGCCTCGCAAAGACTATGAGACgg < 1:2070815/68‑1 (MQ=255) ggTTTGCAGCCGGTATGGAAAGACTGGGACAGCTATCTGGGACGCGCCTCGCAAAGACTATGAGACgg < 1:1128472/68‑1 (MQ=255) ggTTTGCAGCCGGTATGGAAAGACTGGGACAGCTATCTGGGACGCGCCTCGCAAAGACTATGAGACgg < 1:2603209/68‑1 (MQ=255) ggTTTGCAGCCGGTATGGAAAGACTGGGACAGCTATCTGGGACGCGCCTCGCAAAGACTATGAGACgg < 1:276196/68‑1 (MQ=255) ggTTTGCAGCCGGTATGGAAAGACTGGGACAGCTATCTGGGACGCGCCTCGCAAAGACTATGAGACgg < 1:3102125/68‑1 (MQ=255) gTTTGCAGCCGGTATGGAAAGACTGGGACAGCTATCTGGGACGCGCCTCGCAAAGACTATGAGACgg < 1:57069/67‑1 (MQ=255) tGGAAAGACTGGGACAGCTATCTGGGACGCGCCTCGCAAAGACTATGAGACGGAATGTAAGAACGTaaa > 1:3173031/1‑69 (MQ=255) tGGAAAGACTGGGACAGCTATCTGGGACGCGCCTCGCAAAGACTATGAGACGGAATGTAAGAACGTaa > 1:1242295/1‑68 (MQ=255) gAAAGACTGGGACAGCTATCTGGGACGCGCCTCGCAAAGACTATGAGACGGAATGTAAGAACGTAAAtt > 1:1742236/1‑69 (MQ=255) aGACTGGGACAGCTATCTGGGTCGCCCATGGCAAAGGCCATGAGACAGTATGTAAGAACGTAAATTTAt < 1:2339711/69‑1 (MQ=255) aGACTGGGACAGCTATCTGGGACGCCCATCGCAAAGGCCATGAGACAGAATGTAAGAACGTAAATTTAt < 1:3193288/69‑1 (MQ=255) aGACTGGGACAGCTATCTGGGACGCCCATCGCAAAGGCCATGAGACAGAATGTAAGAACGTAAATTTAt < 1:2544968/69‑1 (MQ=255) aGACTGGGACAGCTATCTGGGACGCCCATCGCAAAGGCCATGAGACAGAATGTAAGAACGTAAATTTAt < 1:969695/69‑1 (MQ=255) aGACTGGGACAGCTATCTGGGACGCCCATCGCAAAGGCCATGAGACAGAATGTAAGAACGTAAATTTAt < 1:2147340/69‑1 (MQ=255) aGACTGGGACAGCTATCTGGGACGCCCATCGCAAAGGCCATGAGACAGAATGTAAGAACGTAAATTTAt < 1:2661622/69‑1 (MQ=255) aGACTGGGACAGCTATCTGGGACGCCCATCGCAAAGGCCATGAGACAGAATGTAAGAACGTAAATTTAt < 1:361069/69‑1 (MQ=255) aGACTGGGACAGCTATCTGGGACGCCCATCGCAAAGGCCATGAGACAGAATGTAAGAACGTAAATTTAt < 1:1677703/69‑1 (MQ=255) aGACTGGGACAGCTATCTGGGACGCCCATCGCAAAGGCCATGAGACAGAATGTAAGAACGTAAATTTAt < 1:907724/69‑1 (MQ=255) aGACTGGGACAGCTATCTGGGACGCCCATCGCAAAGGCCATGAGACAGAATGTAAGAACGTAAATTTAt < 1:1385935/69‑1 (MQ=255) aGACTGGGACAGCTATCTGGGACGCCCATCGCAAAGGCCATGAGACAGAATGTAAGAACGTAAATTTAt < 1:1348890/69‑1 (MQ=255) aCAGCTATCTGGGACGCGCCTCGCAAAGACTATGAGACGGAATGTAAGAACGTa < 1:219332/54‑1 (MQ=255) cAGCTATCTGGGACGCGCCTCGCAAAGACTATGAGACGGAATGTAAGAACGTa < 1:1475545/53‑1 (MQ=255) cgcCTCGCAAAGACTATGAGACGGAATGTAAGAACGTAAATTTATTCCGAGCCGCTACGGAAGATGaaa > 1:308216/1‑69 (MQ=255) cgcCTCGCAAAGACTATGAGACGGAATGTAAGAACGTAAATTTATTCCGAGCCGCTACGGAAGATGaaa > 1:2945229/1‑69 (MQ=255) cgcCTCGCAAAGACTATGAGACGGAATGTAAGAACGTAAATTTATTCCGAGCCGCTACGGAAGATGaaa > 1:825059/1‑69 (MQ=255) cgcCTCGCAAAGACTATGAGACGGAATGTAAGAACGTAAATTTATTCCGAGCCGCTACGGAAGATGaaa > 1:1344070/1‑69 (MQ=255) cgcCTCGCAAAGACTATGAGACGGAATGTAAGAACGTAAATTTATTCCGAGCCGCTACGGAAGATGaaa > 1:207015/1‑69 (MQ=255) cgcCTCGCAAAGACTATGAGACGGAATGTAAGAACGTAAATTTATTCCGAGCCGCTACGGAAGATGaaa > 1:3380532/1‑69 (MQ=255) cgcCTCGCAAAGACTATGAGACGGAATGTAAGAACGTAAATTTATTCCGAGCCGCTACGGAAGATGaaa > 1:594730/1‑69 (MQ=255) cgcCTCGCAAAGACTATGAGACGGAATGTAAGAACGTAAATTTATTCCGAGCCGCTACGGAAGATGaaa > 1:173149/1‑69 (MQ=255) ccTAGCAAAGACTATGAGACGGAATGTAAGAACGTAAATTTATTCCGAGCCGCTACGGAAGATGaaaaa < 1:3315495/69‑1 (MQ=255) cTCGCAAAGACTATGAGACGGAATGTAAGAACGTAAATTTATTCCGAGCCGCTACGGAAGATGaaaaa < 1:2522185/68‑1 (MQ=255) tCGCAAAGGCCATGAGACAGATTGTAAGAACGTAAATTTATTCCGAGCAGCTCCGGAAGATGAAAAAc > 1:109259/1‑68 (MQ=255) tCGCAAAGGCCATGAGACAGAATGTAAGAACGTAAATTTATTCCGAGCAGCTCCGGAAGATGaaaaa > 1:1633103/1‑67 (MQ=255) tCGCAAAGGCCATGAGACAGAATGTAAGAACGTAAATTTATTCCGAGCAGCTCCGGAAGATGAAAAAc > 1:1179340/1‑68 (MQ=255) tCGCAAAGGCCATGAGACAGAATGTAAGAACGTAAATTTATTCCGAGCAGCTCCGGAAGATGAAAAAc > 1:115722/1‑68 (MQ=255) tCGCAAAGGCCATGAGACAGAATGTAAGAACGTAAATTTATTCCGAGCAGCTCCGGAAGATGAAAAAc > 1:664937/1‑68 (MQ=255) tCGCAAAGGCCATGAGACAGAATGTAAGAACGTAAATTTATTCCGAGCAGCTCCGGAAGATGAAAAAc > 1:771131/1‑68 (MQ=255) tCGCAAAGGCCATGAGACAGAATGTAAGAACGTAAATTTATTCCGAGCAGCTCCGGAAGATGAAAAAc > 1:2677497/1‑68 (MQ=255) tCGCAAAGGCCATGAGACAGAATGTAAGAACGTAAATTTATTCCGAGCAGCTCCGGAAGATGAAAAAc > 1:2000144/1‑68 (MQ=255) gcGCAAAGGCCATGAGACAGAATGTAAGAACGTAAATTTATTCCGAGCAGCTCCGGAAGATGAAAAAc > 1:3118939/2‑68 (MQ=255) cGCAAAGACTATGAGACGGAATGTAAGAACGTAAATTTATTCCGAGCCGCTACGGAAGATGAAAAAc > 1:3134089/1‑67 (MQ=255) aaaGACTATGAGACGGAATGTAAGAACGTAAATTTATTCCGAGcc < 1:876554/45‑1 (MQ=255) aaaGACTATGAGACGGAATGTAAGAACGTAAATTTATTCCGAGcc < 1:920200/45‑1 (MQ=255) aaaGACTATGAGACGGAATGTAAGAACGTAAATTTATTCCGAGcc < 1:101701/45‑1 (MQ=255) aaaGACTATGAGACGGAATGTAAGAACGTAAATTTATTCCGAGcc < 1:574854/45‑1 (MQ=255) aaaGACTATGAGACGGAATGTAAGAACGTAAATTTATTCCGAGc < 1:1194116/44‑1 (MQ=255) aaGACTATGAGACGGAATGTAAGAACGTAAATTTATTcc > 1:1735797/1‑39 (MQ=255) aaGACTATGAGACGGAATGTAAGAACGTAAATTTATTCCg > 1:1758718/1‑40 (MQ=255) aaGACTATGAGACGGAATGTAAGAACGTAAATTTATTCCGAGCCGCTACTGAAGATGAAAAACGCAAgg > 1:97365/1‑69 (MQ=255) aaGACTATGAGACGGAATGTAAGAACGTAAATTTATTCCGAGCCGCTACGGAAGATGAAAAACGCAAgg > 1:1698339/1‑69 (MQ=255) aaGACTATGAGACGGAATGTAAGAACGTAAATTTATTCCGAGCCGCTACGGAAGATGAAAAACGCAAgg > 1:355004/1‑69 (MQ=255) aaGACTATGAGACGGAATGTAAGAACGTAAATTTATTCCGAGCCGCTACGGAAGATGAAAAACGCAAgg > 1:3349693/1‑69 (MQ=255) aaGACTATGAGACGGAATGTAAGAACGTAAATTTATTCCGAGCCGCTACGGAAGATGAAAAACGCAAgg > 1:1871742/1‑69 (MQ=255) aaGACTATGAGACGGAATGTAAGAACGTAAATTTATTCCGAGCCGCTACGGAAGATGAAAAACGCAAgg > 1:1641012/1‑69 (MQ=255) aaGACTATGAGACGGAATGTAAGAACGTAAATTTATTCCGAGCCGCTACGGAAGATGAAAAACGCAAgg > 1:579835/1‑69 (MQ=255) aaGACTATGAGACGGAATGTAAGAACGTAAATTTATTCCGAGCCGCTACGGAAGATGAAAAACGCAAgg > 1:1305405/1‑69 (MQ=255) aaGACTATGAGACGGAATGTAAGAACGTAAATTTATTCCGAGCCGCTACGGAAGATGAAAAACGCAAgg > 1:887197/1‑69 (MQ=255) aaGACTATGAGACGGAATGTAAGAACGTAAATTTATTCCGAGCCGCTACGGAAGATGAAAAACGCAAgg > 1:1023693/1‑69 (MQ=255) aaGACTATGAGACGGAATGTAAGAACGTAAATTTATTCCGAGCCGCTACGGAAGATGAAAAACGCAAgg > 1:102148/1‑69 (MQ=255) aaGACTATGAGACGGAATGTAAGAACGTAAATTTATTCCGAGCCGCTACGGAAGATGAAAAACGCAAgg > 1:3069304/1‑69 (MQ=255) aaGACTATGAGACGGAATGTAAGAACGTAAATTTATTCCGAGCCGCTACGGAAGATGAAAAACGCAAg > 1:106152/1‑68 (MQ=255) aaGACTATGAGACGGAATGTAAGAACGTAAATTTATTCCGAGCCGCTACGGAAGATGAAAAACGCAAg > 1:3067248/1‑68 (MQ=255) aaGACTATGAGACGGAATGTAAGAACGTAAATTTATTCCGAGCCGCTACGGAAGATGAAAAACGCAAg > 1:303876/1‑68 (MQ=255) gACTATGAGACGGAATGTAAGAACGTAAATTTATTCCGAGCCGCTACGGAAGATGAAAAACGCAAg > 1:1008803/1‑66 (MQ=255) aCTATGAGACGGAATGTAAGAACGTAAATTTATTCCGAGCCGCTACGGAAGATGAAAAACGCAAg > 1:279425/1‑65 (MQ=255) aCTATGAGACGGAATGTAAGAACGTAAATTTATTCCGAGCCGCTACGGAAGATGAAAAACGCAAGgttg > 1:275504/1‑69 (MQ=255) tATGAGACGGAATGTAAGAACGTAAATTTATTCCGAGCCGCTACGGa > 1:3229292/1‑47 (MQ=255) tATGAGACGGAATGTAAGAACGTAAATTTATTCCGAGCCGCTACGGa < 1:260070/47‑1 (MQ=255) tATGAGACGGAATGTAAGAACGTAAATTTATTCCGAGCCGCTACGGa > 1:1566909/1‑47 (MQ=255) tATGAGACGGAATGTAAGAACGTAAATTTATTCCGAGCCGCTACGGa < 1:1494157/47‑1 (MQ=255) tATGAGACGGAATGTAAGAACGTAAATTTATTCCGAGCCGCTACGGa < 1:2649076/47‑1 (MQ=255) tATGAGACGGAATGTAAGAACGTAAATTTATTCCGAGCCGCTACGGa > 1:1281199/1‑47 (MQ=255) tATGAGACGGAATGTAAGAACGTAAATTTATTCCGAGCCGCTACGGa < 1:1101626/47‑1 (MQ=255) tATGAGACGGAATGTAAGAACGTAAATTTATTCCGAGCCGCTACGGa < 1:3111556/47‑1 (MQ=255) tATGAGACGGAATGTAAGAACGTAAATTTATTCCGAGCCGCTACGGa > 1:2924156/1‑47 (MQ=255) tATGAGACGGAATGTAAGAACGTAAATTTATTCCGAGCCGCTACGGa > 1:2997378/1‑47 (MQ=255) tATGAGACGGAATGTAAGAACGTAAATTTATTCCGAGCCGCTACGGa < 1:952811/47‑1 (MQ=255) tATGAGACGGAATGTAAGAACGTAAATTTATTCCGAGCCGCTACGGa < 1:993033/47‑1 (MQ=255) | CAGGGTTTGCAGCCGGTATGGAAAGACTGGGACAGCTATCTGGGACGCGCCTCGCAAAGACTATGAGACGGAATGTAAGAACGTAAATTTATTCCGAGCCGCTACGGAAGATGAAAAACGCAAGGTTG > minE/2251587‑2251714 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |